FastQCFastQC Report
Tue 7 Mar 2017
1HL_28A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_28A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3770238
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTCA134400.081.4447564
TGCGCCT32300.079.8312151
TCACCGG143950.078.5523158
CACCGGC141350.076.90899
TGTGCCT50450.076.4040761
GTGCAGC2400.076.278912
GCGCCTT31200.074.924842
CTTCACC155300.074.564416
GTGCCTT47500.073.8206942
GTGCTGC2250.073.019132
TGCCTTC81400.072.311433
TGCGACT18300.070.307471
CCTTCAC174500.069.722575
TGCTGCG1600.069.493731
TGCGAAC16200.067.655271
CGGGGCA15100.066.8336894
CGCCTTC53000.066.7773743
TTCACCG176450.065.9460457
TGCGATG28500.065.023371
CTGCTGC1900.064.234882
Produced by FastQC (version 0.11.5)