FastQCFastQC Report
Tue 7 Mar 2017
1HL_29A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_29A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3071205
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA153700.5004550331221784No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG94390.30733865046455705No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG70410.22925854835479884No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA68700.2236907012068553No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG61490.20021457375850846No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT58300.18982777118427457No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA56320.1833807902761294No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA56000.1823388539677423No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG55410.18041778389915358No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG54770.17833391128237938No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC53500.1741987265584681No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT52390.17058450998875035No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC45870.1493550577053632No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA45440.14795495579096804No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT45160.14704326152112931No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA44070.14349416597068576No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA42000.13675414047580672No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC38290.1246741914004438No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA38220.12444626783298413No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA36730.11959475189705669No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG34740.11311521047927442No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA34440.11213839519016151No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA32920.10718919772532279No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCTC159.82987E-485.9998863
GCAGCCG357.2759576E-1085.9998863
GCAGCCA700.085.9998863
GCAGCAA1250.085.9998863
TGCAGCT402.1827873E-1185.9998862
AGCAGCC1500.085.9998862
CAGCAGC6650.084.706661
AGCAGCA2950.084.542262
CAGCTGC5700.083.7367251
AGCTGCC2500.082.559892
GCTGCCC1950.081.589643
GCTGCAA800.080.624893
CAGCCCT179850.080.285681
AGCTGCT1450.080.0688552
CTGCTGC2150.079.999891
AGCTGCA1400.079.857042
GCAGCAC650.079.384513
CAGCGTA70100.079.375081
GCAGCTA600.078.833233
GCAGCCC600.078.833233
Produced by FastQC (version 0.11.5)