##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_29A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 3071205 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.814566269591252 12.0 12.0 12.0 2.0 32.0 2 14.565234167045183 12.0 12.0 12.0 12.0 22.0 3 13.738394213346227 12.0 12.0 12.0 12.0 22.0 4 13.717850159790702 12.0 12.0 12.0 12.0 22.0 5 22.440319027873425 22.0 22.0 22.0 22.0 27.0 6 26.77964479740037 27.0 22.0 32.0 22.0 32.0 7 34.94098212265219 37.0 37.0 37.0 27.0 41.0 8 37.58587720455001 41.0 37.0 41.0 27.0 41.0 9 38.51860654042957 41.0 37.0 41.0 32.0 41.0 10-11 38.87987434899331 41.0 41.0 41.0 37.0 41.0 12-13 39.061397073787 41.0 41.0 41.0 37.0 41.0 14-15 39.015664047173665 41.0 41.0 41.0 37.0 41.0 16-17 38.91266848028705 41.0 41.0 41.0 37.0 41.0 18-19 38.8415055328446 41.0 41.0 41.0 37.0 41.0 20-21 38.85139464802903 41.0 41.0 41.0 37.0 41.0 22-23 38.850479697708224 41.0 41.0 41.0 34.5 41.0 24-25 38.871392336232844 41.0 41.0 41.0 37.0 41.0 26-27 38.82967809052147 41.0 41.0 41.0 37.0 41.0 28-29 38.78673615079423 41.0 41.0 41.0 34.5 41.0 30-31 38.6646584972348 41.0 41.0 41.0 32.0 41.0 32-33 38.713546800034514 41.0 41.0 41.0 32.0 41.0 34-35 38.680587749759454 41.0 41.0 41.0 32.0 41.0 36-37 38.60378108918161 41.0 41.0 41.0 32.0 41.0 38-39 38.498140306492076 41.0 41.0 41.0 32.0 41.0 40-41 38.553448239371846 41.0 41.0 41.0 32.0 41.0 42-43 38.29146214596551 41.0 41.0 41.0 32.0 41.0 44-45 38.400995700384705 41.0 41.0 41.0 32.0 41.0 46-47 38.4201516994144 41.0 41.0 41.0 32.0 41.0 48-49 38.353616740009215 41.0 41.0 41.0 32.0 41.0 50-51 38.23185948186461 41.0 41.0 41.0 32.0 41.0 52-53 38.19569419820559 41.0 39.0 41.0 32.0 41.0 54-55 38.0739988050293 41.0 37.0 41.0 32.0 41.0 56-57 37.87513142235703 41.0 37.0 41.0 27.0 41.0 58-59 37.873123741332805 41.0 37.0 41.0 29.5 41.0 60-61 37.87313220706531 41.0 37.0 41.0 29.5 41.0 62-63 37.86741360475774 41.0 37.0 41.0 29.5 41.0 64-65 37.84708379935563 41.0 37.0 41.0 27.0 41.0 66-67 37.70788273658059 41.0 37.0 41.0 27.0 41.0 68-69 37.73376345766564 41.0 37.0 41.0 27.0 41.0 70-71 37.70377164663381 41.0 37.0 41.0 27.0 41.0 72-73 37.56516285952908 41.0 37.0 41.0 27.0 41.0 74-75 37.482423999700444 41.0 37.0 41.0 27.0 41.0 76-77 36.395156135783836 39.0 34.5 41.0 24.5 41.0 78-79 36.935004827095554 41.0 37.0 41.0 27.0 41.0 80-81 37.60734809301235 41.0 37.0 41.0 27.0 41.0 82-83 37.79497216890439 41.0 37.0 41.0 27.0 41.0 84-85 37.72786821459329 41.0 37.0 41.0 27.0 41.0 86-87 37.58055974772117 41.0 37.0 41.0 27.0 41.0 88-89 37.50772612052924 41.0 37.0 41.0 27.0 41.0 90-91 37.34862635349968 41.0 37.0 41.0 27.0 41.0 92-93 37.23552025996311 41.0 37.0 41.0 27.0 41.0 94-95 37.07487842719714 41.0 37.0 41.0 27.0 41.0 96-97 37.01598330297065 41.0 37.0 41.0 27.0 41.0 98-99 36.89680711642499 41.0 37.0 41.0 27.0 41.0 100 36.76748670310187 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3485961525465093 1101 2 -0.5223029493730671 1101 3 -0.02510297941595141 1101 4 0.0920212139019867 1101 5 -1.0906358780386398 1101 6 -0.7568989355192208 1101 7 -0.10775193655879178 1101 8 -0.04142988361854805 1101 9 -0.08714082944030821 1101 10-11 -0.05025945259406939 1101 12-13 -0.005378003742244175 1101 14-15 0.001961943723600257 1101 16-17 -0.01634298099533993 1101 18-19 0.15800820764714985 1101 20-21 0.15666079827774837 1101 22-23 0.14260756789062157 1101 24-25 0.13453820155524454 1101 26-27 0.1982138939776874 1101 28-29 0.06170841692338769 1101 30-31 0.0023415742059000877 1101 32-33 -0.12166807050039807 1101 34-35 0.04062268245638734 1101 36-37 0.002733200248357548 1101 38-39 -0.052389010200442954 1101 40-41 0.02797471995325651 1101 42-43 0.11227390613741761 1101 44-45 0.055325850748310756 1101 46-47 -0.023300393376608497 1101 48-49 0.04415663401184844 1101 50-51 0.037719465054500745 1101 52-53 0.05378020934498551 1101 54-55 -0.06748056567826666 1101 56-57 0.1582442863469069 1101 58-59 0.23125364758757883 1101 60-61 0.1787846439405456 1101 62-63 -0.10128475216094301 1101 64-65 -0.05268457066917165 1101 66-67 -0.14133042106652738 1101 68-69 0.060045982312033175 1101 70-71 -0.08157083142095445 1101 72-73 -0.07341390120036806 1101 74-75 -0.12168985219740591 1101 76-77 -0.5439604928090063 1101 78-79 -0.10117423837250072 1101 80-81 -0.05679970066997697 1101 82-83 -0.24010795847419075 1101 84-85 -0.06772064468144379 1101 86-87 -0.07785787431211588 1101 88-89 -0.27632705624588993 1101 90-91 0.03575943360135625 1101 92-93 -0.017054501748916095 1101 94-95 0.009429915484020057 1101 96-97 -0.1152656569766819 1101 98-99 -0.3350116740033684 1101 100 -0.5949815484331964 1102 1 1.9774419591415047 1102 2 0.8615450101791176 1102 3 -0.09494386893452322 1102 4 0.02091285765007811 1102 5 -0.7866695752911887 1102 6 -0.16375749317680288 1102 7 -0.049918992146757546 1102 8 -0.22147266496143914 1102 9 -0.30719562567333014 1102 10-11 -0.21809722379722984 1102 12-13 -0.3565149040394928 1102 14-15 -0.2501337170259035 1102 16-17 -0.2841335260407547 1102 18-19 -0.21893813510364168 1102 20-21 -0.22997558808778962 1102 22-23 -0.2304686606839823 1102 24-25 -0.09839527206755605 1102 26-27 -0.1949716492620297 1102 28-29 -0.3280471529852207 1102 30-31 -0.25597769545546356 1102 32-33 -0.2930122102898238 1102 34-35 -0.0896390407427674 1102 36-37 -0.3287301855893574 1102 38-39 -0.3844531405446361 1102 40-41 -0.25512873311227935 1102 42-43 -0.25358904771985635 1102 44-45 -0.1158707072981926 1102 46-47 -0.529756660565532 1102 48-49 -0.44259052547366196 1102 50-51 -0.20945825114407768 1102 52-53 -0.23251448187928503 1102 54-55 -0.297337394025071 1102 56-57 -0.27680412046477443 1102 58-59 -0.18573075389031857 1102 60-61 -0.18797942667645628 1102 62-63 -0.4238559039235881 1102 64-65 -0.39754490625269057 1102 66-67 -0.34649161505491577 1102 68-69 -0.38162971288478786 1102 70-71 -0.4105973822446387 1102 72-73 -0.19306362895546414 1102 74-75 -0.40843770618523934 1102 76-77 -0.2839162530112418 1102 78-79 -0.4980823140454689 1102 80-81 -0.4559805351839685 1102 82-83 -0.3832839523070035 1102 84-85 -0.4141878850070242 1102 86-87 -0.3768281009976704 1102 88-89 -0.40240093045763103 1102 90-91 -0.6094123862725169 1102 92-93 -0.3369153582091755 1102 94-95 -0.3214900222885717 1102 96-97 -0.3192279663357951 1102 98-99 -0.4603146506403135 1102 100 -0.6316686334680668 1103 1 1.7220194317168414 1103 2 0.8474794702075759 1103 3 0.3430361045287249 1103 4 1.0158924022365134 1103 5 2.892179035661698 1103 6 -0.2671202950379481 1103 7 -0.4646608786686315 1103 8 -0.30382371403432984 1103 9 -0.29748656386369277 1103 10-11 -0.35358146089909326 1103 12-13 -0.4801110026007436 1103 14-15 -0.3577819364053312 1103 16-17 -0.42972205615483716 1103 18-19 -0.49522261557076064 1103 20-21 -0.2598869535466761 1103 22-23 -0.24375462770068168 1103 24-25 -0.2643481911103578 1103 26-27 -0.35220190920158245 1103 28-29 -0.33191116273769694 1103 30-31 -0.2260867826179691 1103 32-33 -0.4084105072914852 1103 34-35 -0.43338680700937005 1103 36-37 -0.3340890516122599 1103 38-39 -0.28912954517203815 1103 40-41 -0.38832988527196477 1103 42-43 -0.28538100728481197 1103 44-45 -0.5353897119019209 1103 46-47 -0.6753167105788265 1103 48-49 -0.43551475612004253 1103 50-51 -0.36178818864421913 1103 52-53 -0.4205752885697649 1103 54-55 -0.37454240998509647 1103 56-57 -0.42282843800337844 1103 58-59 -0.5937636221603597 1103 60-61 -0.518416654859017 1103 62-63 -0.3968557213158874 1103 64-65 -0.5513068762715179 1103 66-67 -0.739325044438786 1103 68-69 -0.5490368621718957 1103 70-71 -0.5451421948455746 1103 72-73 -0.4642809550202287 1103 74-75 -0.4962808076347045 1103 76-77 -0.7172673724467344 1103 78-79 -0.7117165759417787 1103 80-81 -0.4180024603940993 1103 82-83 -0.6271349728997961 1103 84-85 -0.6197239558923258 1103 86-87 -0.48751817663188035 1103 88-89 -0.437888462316252 1103 90-91 -0.8424114414032857 1103 92-93 -0.30677747822320356 1103 94-95 -0.3820350867429454 1103 96-97 -0.36933360796454906 1103 98-99 -0.4294351711671993 1103 100 -0.40436811144456897 1104 1 1.469111888279535 1104 2 -0.6536489278401056 1104 3 0.004664879868631289 1104 4 0.035413361470167715 1104 5 -1.0987858486801088 1104 6 -0.364861453508059 1104 7 -0.20322220350909959 1104 8 -0.12609799511627529 1104 9 0.008412900663813616 1104 10-11 -0.07397243944749476 1104 12-13 -0.14803616808265474 1104 14-15 -0.06393634277918636 1104 16-17 -0.1468452095414179 1104 18-19 -0.07721378903376319 1104 20-21 0.034666819605178034 1104 22-23 0.14198552113181506 1104 24-25 -0.06498386206923357 1104 26-27 -0.05551163098491685 1104 28-29 0.11535412608530748 1104 30-31 -0.05108629213111726 1104 32-33 0.010594738065343279 1104 34-35 0.09215093007492214 1104 36-37 -0.1140549805171247 1104 38-39 -0.06613467281202645 1104 40-41 -0.09594802963984961 1104 42-43 -0.03284748616110278 1104 44-45 -0.11236400496426313 1104 46-47 -0.08590854105766965 1104 48-49 -0.22145453657951464 1104 50-51 -0.1042530235199095 1104 52-53 -0.11658406139670774 1104 54-55 -0.13839462831450078 1104 56-57 -7.637374306384004E-4 1104 58-59 -0.043480232076653635 1104 60-61 0.08567237747930534 1104 62-63 -0.12339490036195144 1104 64-65 -0.1627338006184118 1104 66-67 -0.49454615521548817 1104 68-69 -0.3991805365543044 1104 70-71 -0.22134239529815858 1104 72-73 -0.260435593766573 1104 74-75 -0.29882562039186666 1104 76-77 -0.27171892674738984 1104 78-79 -0.2282484874337385 1104 80-81 -0.13618986588841864 1104 82-83 -0.11609129619366598 1104 84-85 -0.16051576446970728 1104 86-87 -0.14597019734726757 1104 88-89 -0.14597524773778758 1104 90-91 -0.18663699188607552 1104 92-93 -0.3034000762116946 1104 94-95 -0.27940005223423725 1104 96-97 -0.04158785909409346 1104 98-99 -0.14878228238053737 1104 100 -0.1986295405187093 1105 1 1.6986654428017047 1105 2 0.5486319177870485 1105 3 -0.11353437876219985 1105 4 -0.19990632281788656 1105 5 -1.097045969393502 1105 6 0.38020162216995246 1105 7 -0.0331917926833043 1105 8 -0.03799527889651699 1105 9 0.03867574911978977 1105 10-11 0.07344560529745792 1105 12-13 -0.06219489062581829 1105 14-15 -0.03608708829252549 1105 16-17 0.058984868537521606 1105 18-19 0.1585935601031352 1105 20-21 -0.04552625173997882 1105 22-23 0.030206295775670355 1105 24-25 -0.008490749821419286 1105 26-27 0.14563391308449525 1105 28-29 0.09847642100091747 1105 30-31 0.11038353524886446 1105 32-33 -0.03657283683808288 1105 34-35 0.03402871939050556 1105 36-37 0.03218099751807557 1105 38-39 0.0043560445748482834 1105 40-41 0.06165268895320963 1105 42-43 0.21535377380260456 1105 44-45 0.05321010466160914 1105 46-47 -0.10325012410523016 1105 48-49 -0.18016110950922837 1105 50-51 -0.002609774244795915 1105 52-53 0.044556253600951834 1105 54-55 0.03780635671862598 1105 56-57 0.08294905176028067 1105 58-59 0.06566862194127054 1105 60-61 -0.009533857514917088 1105 62-63 -0.17733257333068053 1105 64-65 -0.0442108247105466 1105 66-67 -0.05012074327446214 1105 68-69 -0.28884388810627826 1105 70-71 -0.3586001379968309 1105 72-73 -0.06648164059507167 1105 74-75 -0.1982526462023273 1105 76-77 -0.15581014979088081 1105 78-79 -0.16831045462939898 1105 80-81 -0.34257998024740743 1105 82-83 -0.19404145963584796 1105 84-85 -0.27747911581953133 1105 86-87 -0.19631450072506595 1105 88-89 -0.19154701236756466 1105 90-91 -0.24869010335223152 1105 92-93 -0.09607701728558027 1105 94-95 -0.21964591294550928 1105 96-97 -0.2934767601871471 1105 98-99 -0.4161218101872777 1105 100 -0.23057633875672678 1106 1 1.5606607456873842 1106 2 -0.5976392421467267 1106 3 -0.2030269448103148 1106 4 -0.17780400014608588 1106 5 -1.2170273103630151 1106 6 -0.6327742235533016 1106 7 -0.2468336471536503 1106 8 -0.30402708014008795 1106 9 -0.433876736144434 1106 10-11 -0.29657213695985263 1106 12-13 -0.10197448756918703 1106 14-15 -0.1323556192444073 1106 16-17 -0.2835515192683147 1106 18-19 -0.1355644376642502 1106 20-21 -0.29797383388555687 1106 22-23 -0.15171392608343837 1106 24-25 -0.38216857415688565 1106 26-27 -0.2746242124918581 1106 28-29 -0.3704889195803176 1106 30-31 -0.25403312737321926 1106 32-33 -0.2712166839418799 1106 34-35 -0.26541872254270515 1106 36-37 -0.3290247344713819 1106 38-39 -0.09474140571717271 1106 40-41 -0.3555524471336824 1106 42-43 -0.14448650543572228 1106 44-45 -0.23431099460335503 1106 46-47 -0.44014343597527983 1106 48-49 -0.2586534760243637 1106 50-51 -0.36859155742255467 1106 52-53 -0.3125434131250344 1106 54-55 -0.4319369602745766 1106 56-57 -0.4191475950070185 1106 58-59 -0.30626888378856165 1106 60-61 -0.1936604018732524 1106 62-63 -0.522647053493337 1106 64-65 -0.6390706258247008 1106 66-67 -0.5425902605664277 1106 68-69 -0.4163897537289003 1106 70-71 -0.5329306239405369 1106 72-73 -0.5903407972716082 1106 74-75 -0.725013389975004 1106 76-77 -0.5082184994373833 1106 78-79 -0.3981571512799462 1106 80-81 -0.4938682487864554 1106 82-83 -0.530668030958104 1106 84-85 -0.7198581691703367 1106 86-87 -0.5010890014881539 1106 88-89 -0.6766363033783378 1106 90-91 -0.7743412145352409 1106 92-93 -0.6634920674654339 1106 94-95 -0.5786999243674273 1106 96-97 -0.4608454324299487 1106 98-99 -0.7499123841412114 1106 100 -0.6685548808716675 1107 1 1.318509814320178 1107 2 -0.48771346743882305 1107 3 -0.2270812082225646 1107 4 -0.27826580236392573 1107 5 -1.2174154488508293 1107 6 -0.2299979597909001 1107 7 -0.08352231384013464 1107 8 -0.0449054127632138 1107 9 -0.16502600594857597 1107 10-11 -0.0763886052629772 1107 12-13 -0.3082618355758129 1107 14-15 -0.23687573968295084 1107 16-17 -0.3265448763193888 1107 18-19 -0.11076982073216612 1107 20-21 -0.22294026403549339 1107 22-23 -0.15978328395372898 1107 24-25 -0.15138473260988405 1107 26-27 -0.136860237028813 1107 28-29 -0.15400591674278274 1107 30-31 -0.31417766353946774 1107 32-33 -0.16009118968468528 1107 34-35 -0.29951854680680867 1107 36-37 -0.14799715021229076 1107 38-39 -0.3553357283246754 1107 40-41 -0.30539339293633105 1107 42-43 -0.22415420108229966 1107 44-45 -0.1080668868759318 1107 46-47 -0.31651368871124674 1107 48-49 -0.25431747361955104 1107 50-51 -0.383695925384437 1107 52-53 -0.13245561950239448 1107 54-55 -0.2410267094545233 1107 56-57 -0.1742757715489205 1107 58-59 -0.34068665128204856 1107 60-61 -0.22646533007588943 1107 62-63 -0.3761916662962861 1107 64-65 -0.2892981823360117 1107 66-67 -0.13118328332289764 1107 68-69 -0.47806351424580384 1107 70-71 -0.329937911034186 1107 72-73 -0.28783350522299145 1107 74-75 -0.26389536944826375 1107 76-77 -0.3852510650823362 1107 78-79 -0.7114280603828647 1107 80-81 -0.348511004470609 1107 82-83 -0.1845091980822744 1107 84-85 -0.14216729587525379 1107 86-87 -0.19404585700483779 1107 88-89 -0.16135211168086272 1107 90-91 -0.09706288290311704 1107 92-93 -0.30110904579904485 1107 94-95 -0.27579520328209384 1107 96-97 -0.2783935037945753 1107 98-99 -0.2568466433912917 1107 100 -0.25084147902776266 1108 1 1.953381646939219 1108 2 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0.17832927065044402 2223 22-23 0.27622981418892323 2223 24-25 0.3181785049138597 2223 26-27 0.3439343706840816 2223 28-29 0.30620101352865703 2223 30-31 0.2697666374056098 2223 32-33 0.28967176337464196 2223 34-35 0.3362167597406014 2223 36-37 0.20786140537656195 2223 38-39 0.21955031807691228 2223 40-41 0.3423481399604782 2223 42-43 0.4920138844689461 2223 44-45 0.4294681403835554 2223 46-47 0.3376602422319195 2223 48-49 0.30381716127800473 2223 50-51 0.37917847552758843 2223 52-53 0.43279790526408846 2223 54-55 0.3579437752390291 2223 56-57 0.48579936038085236 2223 58-59 0.2832579812820555 2223 60-61 0.28875214123198845 2223 62-63 0.4052157895508657 2223 64-65 0.2750510739678873 2223 66-67 0.36932324451671406 2223 68-69 0.3850730679025034 2223 70-71 0.5397726138661554 2223 72-73 0.5044120287382299 2223 74-75 0.46733145513375973 2223 76-77 0.5172529416438678 2223 78-79 0.6413320815986054 2223 80-81 0.3935433834759223 2223 82-83 0.34313508955759175 2223 84-85 0.42993193747818026 2223 86-87 0.587183656926598 2223 88-89 0.4710546700090745 2223 90-91 0.5382874220448457 2223 92-93 0.49666886408712685 2223 94-95 0.7676458779253394 2223 96-97 0.57018035241299 2223 98-99 0.7124783946134556 2223 100 0.6133969275509088 2224 1 1.2016163785415799 2224 2 -0.8972922508257728 2224 3 -0.09493318042338927 2224 4 -0.33904413178112236 2224 5 -0.957204770420649 2224 6 0.49130501323630327 2224 7 0.4015765443309931 2224 8 0.48405457791573525 2224 9 0.4054223754895858 2224 10-11 0.3785098152286679 2224 12-13 0.3172599788144268 2224 14-15 0.31031195113430954 2224 16-17 0.33296445767850713 2224 18-19 0.31967304251376305 2224 20-21 0.36366966450311367 2224 22-23 0.3839326116763857 2224 24-25 0.4909307531744318 2224 26-27 0.3878888955955162 2224 28-29 0.2993673816797653 2224 30-31 0.29954591332792546 2224 32-33 0.4800642852466481 2224 34-35 0.4420748406404087 2224 36-37 0.3349654312660064 2224 38-39 0.431871208738805 2224 40-41 0.2966079361766347 2224 42-43 0.5385311681025655 2224 44-45 0.5323661159052833 2224 46-47 0.534278209633186 2224 48-49 0.35751721471855546 2224 50-51 0.4679058337483539 2224 52-53 0.4682414096280496 2224 54-55 0.49374924277076815 2224 56-57 0.6969010732965586 2224 58-59 0.6917276036703015 2224 60-61 0.5120731607983302 2224 62-63 0.4712406347089626 2224 64-65 0.5388199738649746 2224 66-67 0.508979406253701 2224 68-69 0.5984447646607904 2224 70-71 0.6989972666591555 2224 72-73 0.7163202425087718 2224 74-75 0.5186121364798879 2224 76-77 0.6752739426312644 2224 78-79 0.5362299443941723 2224 80-81 0.49145016050984935 2224 82-83 0.5259844832369964 2224 84-85 0.46882422293838033 2224 86-87 0.4723538003601959 2224 88-89 0.648787418210155 2224 90-91 0.5145775494253968 2224 92-93 0.5211380073892045 2224 94-95 0.6214557741337785 2224 96-97 0.6197053630071565 2224 98-99 0.5707140971792342 2224 100 0.6550784678077193 2225 1 1.1427423783185304 2225 2 1.7054551499119714 2225 3 0.8404303585713304 2225 4 0.9510837218311199 2225 5 3.2214939834485214 2225 6 0.1443124454113942 2225 7 0.27669188297683434 2225 8 0.5788474260253906 2225 9 0.3785049395479021 2225 10-11 0.33300714126589526 2225 12-13 0.19297022551550214 2225 14-15 0.31695358962168996 2225 16-17 0.3969900315573085 2225 18-19 0.2822316455931073 2225 20-21 0.3909206882157932 2225 22-23 0.3607473081082162 2225 24-25 0.32488433311766585 2225 26-27 0.22804603759593078 2225 28-29 0.31652469570178 2225 30-31 0.24062167437887183 2225 32-33 0.20599612155361768 2225 34-35 0.23058650654604662 2225 36-37 0.3149333968448431 2225 38-39 0.33531040462459316 2225 40-41 0.21570254490375618 2225 42-43 0.5443315052017823 2225 44-45 0.2982614120875553 2225 46-47 0.33247194331217855 2225 48-49 0.34663421781810655 2225 50-51 0.30824334855694246 2225 52-53 0.2947810293077424 2225 54-55 0.4296619226043603 2225 56-57 0.37886448950888507 2225 58-59 0.40636177631476045 2225 60-61 0.4395918964237424 2225 62-63 0.38561221137378965 2225 64-65 0.44995858329912153 2225 66-67 0.5063824455125001 2225 68-69 0.5450595425267082 2225 70-71 0.4812158087997105 2225 72-73 0.47414722559700095 2225 74-75 0.4912806255935962 2225 76-77 0.6043832205403206 2225 78-79 0.5760609686750016 2225 80-81 0.3961393406138427 2225 82-83 0.38747315927033554 2225 84-85 0.28445855392689623 2225 86-87 0.36360195125944017 2225 88-89 0.3699310567774958 2225 90-91 0.5672876393606145 2225 92-93 0.5238159156064697 2225 94-95 0.45407013693838394 2225 96-97 0.666967773287638 2225 98-99 0.583290678357649 2225 100 0.6393303368987517 2226 1 1.5086973033970708 2226 2 1.606320465937232 2226 3 0.9527546808482867 2226 4 0.8097997988547156 2226 5 3.114327021486865 2226 6 -0.44836261682149825 2226 7 0.0543944634061404 2226 8 0.1610776993030214 2226 9 0.25874607491154933 2226 10-11 0.03566299441941112 2226 12-13 0.15270073025951802 2226 14-15 0.10023247136551561 2226 16-17 0.14401937097330375 2226 18-19 0.1590877823981529 2226 20-21 0.06182717895397616 2226 22-23 0.09076064635846137 2226 24-25 0.07131370115131119 2226 26-27 0.15938456033539694 2226 28-29 0.14763327763352407 2226 30-31 0.12541296535104607 2226 32-33 0.015649545362258266 2226 34-35 0.04738553428202863 2226 36-37 0.12542785901167264 2226 38-39 -0.02784699935368451 2226 40-41 -0.029801023360938927 2226 42-43 0.21103839353609288 2226 44-45 0.09685311012493258 2226 46-47 0.07347618651180454 2226 48-49 0.15665016269543486 2226 50-51 0.16841161409517014 2226 52-53 0.20722262350088272 2226 54-55 0.2683157167592114 2226 56-57 0.3311135010422248 2226 58-59 0.1646322279477559 2226 60-61 0.20878558854398932 2226 62-63 0.3565368774835278 2226 64-65 0.20193413571468 2226 66-67 0.29926119816121854 2226 68-69 0.4192540132156992 2226 70-71 0.3460348389134822 2226 72-73 0.29959336389606506 2226 74-75 0.43082311913883586 2226 76-77 0.4626655611283681 2226 78-79 0.301222718960652 2226 80-81 0.15077096975840476 2226 82-83 0.20409141965317446 2226 84-85 0.23797162178231446 2226 86-87 0.2660676731925662 2226 88-89 0.21742903670726577 2226 90-91 0.22808911011904343 2226 92-93 0.1882261576782227 2226 94-95 0.3394832307811768 2226 96-97 0.23350594104184097 2226 98-99 0.20817797001160443 2226 100 0.4802229764782382 2227 1 1.5698790347729297 2227 2 1.5723460085907366 2227 3 0.6432119932427831 2227 4 -0.01187479660626245 2227 5 3.1155685598434744 2227 6 0.05638506848209701 2227 7 0.3541988012239301 2227 8 0.32244784611359734 2227 9 0.38212621370807653 2227 10-11 0.22315500168321023 2227 12-13 0.24500191361075707 2227 14-15 0.21683317131606827 2227 16-17 0.28945733028920273 2227 18-19 0.0919429369670226 2227 20-21 0.17750551714689777 2227 22-23 0.32505598542098824 2227 24-25 0.3097562046041702 2227 26-27 0.2117327196615406 2227 28-29 0.300786891629123 2227 30-31 0.24098899652058492 2227 32-33 0.2820650269725675 2227 34-35 0.2533865871918124 2227 36-37 0.21983219768201678 2227 38-39 0.22882054118548467 2227 40-41 0.41952129917900294 2227 42-43 0.557209659756019 2227 44-45 0.49485567206330927 2227 46-47 0.46361762597161515 2227 48-49 0.4067226301703215 2227 50-51 0.5191296815155013 2227 52-53 0.4743297432171474 2227 54-55 0.31861613707056335 2227 56-57 0.5657344108926878 2227 58-59 0.5183992578043544 2227 60-61 0.5113543473892861 2227 62-63 0.48362138308484504 2227 64-65 0.44205031812810347 2227 66-67 0.49852330875511086 2227 68-69 0.4047754380115123 2227 70-71 0.660284715292498 2227 72-73 0.6091429785396087 2227 74-75 0.6081355775766113 2227 76-77 0.6578950698841481 2227 78-79 0.4432882764486763 2227 80-81 0.397909037349244 2227 82-83 0.4851874262690359 2227 84-85 0.34245746504795704 2227 86-87 0.6261233878923775 2227 88-89 0.47196251668066225 2227 90-91 0.570607163045878 2227 92-93 0.5035433307646571 2227 94-95 0.6624505181104468 2227 96-97 0.5502165144727087 2227 98-99 0.7698147545018585 2227 100 0.6426545586563535 2228 1 1.4626577851276075 2228 2 1.5805698601023348 2228 3 -0.09082346923091755 2228 4 -0.1492289769916173 2228 5 3.2326752952508038 2228 6 0.29624060066011637 2228 7 0.505495719136654 2228 8 0.6426259615272443 2228 9 0.43475002988569145 2228 10-11 0.29662499991651714 2228 12-13 0.4089113247645102 2228 14-15 0.3709926105764083 2228 16-17 0.4667284758063417 2228 18-19 0.318928599934317 2228 20-21 0.3612007561598958 2228 22-23 0.4185887282484515 2228 24-25 0.3297919802654832 2228 26-27 0.4262667660989621 2228 28-29 0.43457963370708086 2228 30-31 0.40878896065123627 2228 32-33 0.3637519839132821 2228 34-35 0.41034102508697856 2228 36-37 0.5118669267078175 2228 38-39 0.4550708824796672 2228 40-41 0.41402713196359286 2228 42-43 0.7310838573800709 2228 44-45 0.5690334207530086 2228 46-47 0.4435910853295084 2228 48-49 0.4990928313063705 2228 50-51 0.38239538506335435 2228 52-53 0.40938835212880775 2228 54-55 0.5611138216237208 2228 56-57 0.7315756140684613 2228 58-59 0.6803595445450128 2228 60-61 0.5860792375059347 2228 62-63 0.5814752449289529 2228 64-65 0.5547506778923292 2228 66-67 0.5967118396643158 2228 68-69 0.6665903066600904 2228 70-71 0.7716324124266976 2228 72-73 0.6555434625081489 2228 74-75 0.6567067594984621 2228 76-77 0.8296412647885631 2228 78-79 0.7331948689139622 2228 80-81 0.5529203067494777 2228 82-83 0.6425106626480499 2228 84-85 0.542328977824404 2228 86-87 0.716625906777324 2228 88-89 0.7343938563205725 2228 90-91 0.5448917836605816 2228 92-93 0.6009259389482224 2228 94-95 0.7975337537399554 2228 96-97 0.6439346938087454 2228 98-99 0.7917342733172035 2228 100 0.9300005747493785 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 18.0 13 43.0 14 163.0 15 561.0 16 2053.0 17 4942.0 18 8240.0 19 10630.0 20 12382.0 21 14473.0 22 16904.0 23 19329.0 24 21666.0 25 24728.0 26 27673.0 27 31153.0 28 35521.0 29 40470.0 30 45516.0 31 53018.0 32 62494.0 33 74627.0 34 91531.0 35 118171.0 36 165421.0 37 273721.0 38 673673.0 39 1240781.0 40 1303.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.347501660465635 18.716643743424264 19.589049449548295 34.346805146561806 2 19.18642173657924 50.898787571319815 12.444440392233735 17.47035029986721 3 39.417883208707984 15.253035860517288 25.465932752779448 19.86314817799528 4 19.769927438904276 15.889203097806886 21.28158165931613 43.05928780397271 5 17.204126718991404 35.225164064267936 26.249794461782916 21.320914754957744 6 17.71334052920596 28.281863307724493 29.93281138836385 24.071984774705694 7 20.19380015336 26.429398232941143 36.06561593902068 17.311185674678182 8 21.32153340464085 28.036454746589694 33.49467065858514 17.14734119018431 9 21.11148555697194 27.932391357789534 27.403869165360177 23.552253919878353 10-11 19.84397003781903 30.006674904475606 28.60105398369695 21.54830107400841 12-13 24.31451173073761 26.675474284523503 27.290119024942978 21.71989495979591 14-15 21.763428361180708 27.472522999929993 28.596088505977296 22.167960132912 16-17 20.931377097914336 29.54092937462657 28.287773040223623 21.239920487235466 18-19 21.320784512919197 29.574173654966046 29.6698038717702 19.435237960344555 20-21 22.732624491038532 28.949370035539797 29.783554012187395 18.534451461234273 22-23 21.86029587735107 30.3261586250348 28.63665890098512 19.17688659662901 24-25 22.185868413212404 28.370769779288587 26.632885137918176 22.810476669580833 26-27 20.24505039552879 26.520860704511744 30.041009961887923 23.193078938071537 28-29 19.789114557581605 27.85039431738834 30.63506092989582 21.725430195134237 30-31 20.13342360014797 30.154831033027495 28.192472164475774 21.51927320234876 32-33 20.862169734680688 29.88441019078831 28.6985238692956 20.554896205235405 34-35 21.49897190190821 29.05151235427137 30.00662606371115 19.442889680109275 36-37 21.785716030027302 28.18779599538292 28.74148746176175 21.285000512828027 38-39 21.555370103794015 29.35970192554716 28.878632603309672 20.20629536734915 40-41 20.95454501793215 29.622916035458267 29.92517972446563 19.49735922214395 42-43 20.66590225356428 29.80935962684311 29.210099044299113 20.3146390752935 44-45 20.651145071722663 30.02948354147639 28.700510060383465 20.61886132641748 46-47 19.973521155561258 28.06194200117156 28.881151601804028 23.083385241463162 48-49 20.99689226165174 28.14729530384577 30.124939579894466 20.73087285460802 50-51 21.991872896794582 30.518754039538226 28.19141021195264 19.297962851714555 52-53 20.32668689133479 30.239075244035902 28.25370616446452 21.18053170016479 54-55 22.335597528518196 28.393203017949915 28.10627469466746 21.164924758864434 56-57 21.311028050287184 28.749981275493823 29.31691047601928 20.622080198199715 58-59 21.156376830519662 27.907200155269944 29.094617948984315 21.841805065226076 60-61 19.599094555284292 30.451862758441493 29.505945237005232 20.443097449268983 62-63 20.30945268739085 31.176345502746646 27.350829951794964 21.163371858067546 64-65 19.990980731488726 30.233255540785738 28.69242584948404 21.083337878241505 66-67 20.217276280808345 30.267321784120565 29.330034953707095 20.18536698136399 68-69 20.940835925963913 29.99615785986282 29.14263294049079 19.92037327368248 70-71 19.69223806291016 30.658406065371736 29.296285985468245 20.35306988624986 72-73 20.04231238227341 30.678593581346735 28.414921830356487 20.86417220602337 74-75 20.093253299600644 29.27655431662816 30.15420657364129 20.475985810129902 76-77 19.644308992724355 30.443995109411453 28.622902085663444 21.288793812200748 78-79 19.48103431714913 29.442987361638185 28.57541258235774 22.500565738854945 80-81 21.20795050276732 29.330519451341853 28.042701196469654 21.41882884942117 82-83 20.375455236625363 28.077139103381242 29.85025421617899 21.6971514438144 84-85 20.677567925293168 28.72637938529014 28.733038009510924 21.86301467990577 86-87 21.262110474553147 28.115511663988563 29.167183564757153 21.455194296701134 88-89 20.59216170851506 28.329255129501284 28.726721270641324 22.351861891342324 90-91 20.621628969736637 28.618213372275704 28.051432581022755 22.708725076964907 92-93 20.493421963040564 28.793177270810645 28.58579938493197 22.12760138121682 94-95 20.80704479186508 28.724735079553465 28.14717675961064 22.321043368970813 96-97 20.395968357696734 28.920456302982057 28.367367206031506 22.316208133289702 98-99 21.83128446326442 29.539578113476633 27.47061821011623 21.158519213142725 100 21.009278117221093 30.934535467349132 26.493998284061142 21.562188131368632 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 10.5 2 18.5 3 17.0 4 23.5 5 30.0 6 35.0 7 44.5 8 51.0 9 66.0 10 82.5 11 99.5 12 106.0 13 111.5 14 136.0 15 179.0 16 255.0 17 328.5 18 465.5 19 785.5 20 1264.5 21 2019.5 22 3159.0 23 4787.0 24 7302.0 25 11004.5 26 15923.0 27 21589.5 28 27746.5 29 34763.5 30 43107.5 31 52722.0 32 63558.5 33 75351.0 34 87932.0 35 102213.5 36 116521.5 37 127484.0 38 135145.5 39 141896.5 40 147330.5 41 148091.5 42 146317.5 43 145058.0 44 144774.5 45 141670.5 46 131466.0 47 120947.0 48 114249.5 49 109567.5 50 105643.0 51 99052.5 52 87212.5 53 73442.0 54 61162.5 55 49344.5 56 38177.5 57 28234.5 58 19919.0 59 14114.0 60 10699.0 61 8801.5 62 7343.0 63 5875.0 64 4851.0 65 4824.5 66 4540.0 67 3239.5 68 2312.5 69 2653.0 70 3747.5 71 3744.5 72 2151.0 73 774.0 74 309.5 75 176.0 76 126.0 77 90.5 78 67.5 79 62.5 80 54.5 81 37.5 82 30.5 83 30.0 84 28.5 85 22.5 86 23.0 87 27.0 88 18.0 89 14.5 90 15.0 91 10.5 92 13.0 93 14.0 94 12.0 95 15.0 96 18.5 97 21.0 98 50.0 99 111.0 100 107.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.366125022588854 2 0.005828331225040334 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.7444586082661366E-4 30-31 0.010061197477862924 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.465732505645178E-4 40-41 0.0014815031884879062 42-43 1.1396178372983895E-4 44-45 0.0 46-47 0.0024094777131451663 48-49 1.628025481854842E-5 50-51 0.0 52-53 0.0012372993662096798 54-55 0.018803694315423426 56-57 0.011819464998266152 58-59 0.01380565608612906 60-61 2.279235674596779E-4 62-63 0.01185202550790325 64-65 8.14012740927421E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.768152891129053E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3071205.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.20557738456252 #Duplication Level Percentage of deduplicated Percentage of total 1 85.61512523540425 62.675046757093966 2 8.863361072027399 12.976949296912412 3 2.5382003992140962 5.57431277226585 4 1.0671580425369576 3.1248768265799005 5 0.5525048044967406 2.022321661046436 6 0.33087287162083956 1.4533043764735072 7 0.2178575660577778 1.1163872237598564 8 0.15642737048948746 0.9161084780345443 9 0.11051996270636484 0.7281609914195779 >10 0.5093595105656026 6.361822490319086 >50 0.026610813153513053 1.3205112784760762 >100 0.011414529906034991 1.4163302576883905 >500 4.973876947295565E-4 0.24299746660282354 >1k 9.043412617640421E-5 0.07087012332763898 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 3.256050963709684E-5 0.0 0.0 0.0 0.0 42-43 3.256050963709684E-5 0.0 0.0 0.0 0.0 44-45 3.256050963709684E-5 0.0 0.0 0.0 0.0 46-47 3.256050963709684E-5 0.0 0.0 0.0 0.0 48-49 3.256050963709684E-5 0.0 0.0 0.0 0.0 50-51 3.256050963709684E-5 0.0 0.0 0.0 0.0 52-53 3.256050963709684E-5 0.0 0.0 0.0 0.0 54-55 9.768152891129051E-5 0.0 0.0 0.0 0.0 56-57 9.768152891129051E-5 0.0 4.884076445564526E-4 0.0 0.0 58-59 1.4652229336693578E-4 0.0 9.768152891129053E-4 0.0 0.0 60-61 2.2792356745967787E-4 0.0 9.768152891129053E-4 0.0 0.0 62-63 2.767643319153231E-4 0.0 9.768152891129053E-4 0.0 0.0 64-65 3.5816560600806526E-4 0.0 9.768152891129053E-4 0.0 0.0 66-67 4.884076445564526E-4 0.0 9.768152891129053E-4 1.628025481854842E-5 0.0 68-69 5.860891734677431E-4 0.0 0.001009375798750002 4.8840764455645256E-5 0.0 70-71 6.023694282862916E-4 0.0 0.001009375798750002 6.512101927419368E-5 0.0 72-73 6.674904475604852E-4 0.0 0.001009375798750002 6.512101927419368E-5 0.0 74-75 7.814522312903241E-4 0.0 0.001009375798750002 6.512101927419368E-5 0.0 76-77 9.116942698387115E-4 0.0 0.001009375798750002 6.512101927419368E-5 0.0 78-79 0.0010419363083870989 0.0 0.001009375798750002 6.512101927419368E-5 0.0 80-81 0.001514063698125003 0.0 0.001009375798750002 6.512101927419368E-5 0.0 82-83 0.0018559490493145199 0.0 0.001009375798750002 6.512101927419368E-5 0.0 84-85 0.0020024713426814557 0.0 0.001009375798750002 6.512101927419368E-5 0.0 86-87 0.002018751597500004 0.0 0.001009375798750002 1.3024203854838736E-4 0.0 88 0.002018751597500004 0.0 0.001009375798750002 1.3024203854838736E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11845 0.0 80.28509 8 GGCGCGC 510 0.0 78.231064 6 CACCGGC 11910 0.0 76.2211 9 CCGCTGC 35 1.7878483E-6 75.679825 1 CTTCACC 13475 0.0 75.345665 6 CCTTCAC 14890 0.0 70.770485 5 GCAGCCT 90 0.0 70.6345 1 GTGTGGC 4790 0.0 68.88907 7 CGTAGTG 2570 0.0 67.57703 4 CGGGGCA 1070 0.0 67.11787 94 TTCACCG 14880 0.0 66.717224 7 GGCCCGA 885 0.0 64.175995 9 CGGGCCC 880 0.0 64.00723 7 GGGCCCG 885 0.0 63.645615 8 CGCTGCA 85 0.0 62.324562 1 CAGCGTA 1045 0.0 61.847435 1 GGCTGCC 60 9.944415E-9 61.805183 1 GCCTTCA 4755 0.0 61.597706 4 ACCTTCA 5885 0.0 60.457928 4 AACGGGC 935 0.0 60.242104 5 >>END_MODULE