FastQCFastQC Report
Tue 7 Mar 2017
1HL_29A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_29A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3071205
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG118450.080.285098
GGCGCGC5100.078.2310646
CACCGGC119100.076.22119
CCGCTGC351.7878483E-675.6798251
CTTCACC134750.075.3456656
CCTTCAC148900.070.7704855
GCAGCCT900.070.63451
GTGTGGC47900.068.889077
CGTAGTG25700.067.577034
CGGGGCA10700.067.1178794
TTCACCG148800.066.7172247
GGCCCGA8850.064.1759959
CGGGCCC8800.064.007237
GGGCCCG8850.063.6456158
CGCTGCA850.062.3245621
CAGCGTA10450.061.8474351
GGCTGCC609.944415E-961.8051831
GCCTTCA47550.061.5977064
ACCTTCA58850.060.4579284
AACGGGC9350.060.2421045
Produced by FastQC (version 0.11.5)