FastQCFastQC Report
Tue 7 Mar 2017
1HL_2A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_2A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2758150
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA148510.5384406214310317No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG94980.34436125663941414No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA66450.24092235737722748No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT61180.22181534724362342No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG52360.1898373910048402No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA52150.18907601109439298No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG50780.18410891358338016No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG49730.18030201403114407No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT46000.16677845657415297No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA44050.15970850026285735No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC42570.15434258470351503No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA42410.15376248572412668No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT39340.14263183655711256No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA37560.13617823541141708No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC35240.12776680021028586No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA30520.11065388031832932No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA29430.10670195602124613No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT28100.10187988325508039No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA302.2700988E-887.03
GCTGCCG202.6991976E-587.03
TGCTGCT302.2700988E-887.02
AGCTGCG402.0008883E-1187.02
GCAGCTC159.3885115E-487.03
GCAGCAT450.087.03
AGCAGCG1450.087.02
GCTGCTC257.8035737E-786.999993
GCTGCAT500.086.999993
CAGCAGC5600.086.223211
CAGCTGC5100.086.147061
AGCTGCC1750.084.514292
AGCTGCT1600.084.281252
AGCTGCA1450.084.02
CTGCTGC1400.083.892851
AGCAGCA2750.083.8363652
GCTGCCC1100.083.0454563
CAGCCCT165550.081.324371
CAGCGTA65450.080.8854141
AGCCCTT141350.079.552522
Produced by FastQC (version 0.11.5)