##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_2A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2758150 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.781357069049907 12.0 12.0 12.0 2.0 27.0 2 14.613440168228705 12.0 12.0 12.0 12.0 22.0 3 13.707240360386491 12.0 12.0 12.0 12.0 22.0 4 13.72482098508058 12.0 12.0 12.0 12.0 22.0 5 22.448052136395773 22.0 22.0 22.0 22.0 27.0 6 26.80555879846999 27.0 22.0 32.0 22.0 32.0 7 34.986991280387215 37.0 37.0 37.0 27.0 41.0 8 37.60250240197234 41.0 37.0 41.0 27.0 41.0 9 38.55520910755398 41.0 41.0 41.0 32.0 41.0 10-11 38.924878451135726 41.0 41.0 41.0 37.0 41.0 12-13 39.10009734785998 41.0 41.0 41.0 37.0 41.0 14-15 39.052034515889275 41.0 41.0 41.0 37.0 41.0 16-17 38.9679250584631 41.0 41.0 41.0 37.0 41.0 18-19 38.890317422910286 41.0 41.0 41.0 37.0 41.0 20-21 38.909697260845135 41.0 41.0 41.0 37.0 41.0 22-23 38.893324148432825 41.0 41.0 41.0 37.0 41.0 24-25 38.91386436560738 41.0 41.0 41.0 37.0 41.0 26-27 38.87809763790946 41.0 41.0 41.0 37.0 41.0 28-29 38.828171419248406 41.0 41.0 41.0 37.0 41.0 30-31 38.707132679513435 41.0 41.0 41.0 32.0 41.0 32-33 38.75828490111125 41.0 41.0 41.0 32.0 41.0 34-35 38.73033029385638 41.0 41.0 41.0 32.0 41.0 36-37 38.6517089353371 41.0 41.0 41.0 32.0 41.0 38-39 38.5689453075431 41.0 41.0 41.0 32.0 41.0 40-41 38.61266555481029 41.0 41.0 41.0 32.0 41.0 42-43 38.351113971321354 41.0 41.0 41.0 32.0 41.0 44-45 38.4596071642224 41.0 41.0 41.0 32.0 41.0 46-47 38.46839330710802 41.0 41.0 41.0 32.0 41.0 48-49 38.418276562188424 41.0 41.0 41.0 32.0 41.0 50-51 38.28768558635317 41.0 41.0 41.0 32.0 41.0 52-53 38.27766836466472 41.0 41.0 41.0 32.0 41.0 54-55 38.14019306419158 41.0 39.0 41.0 32.0 41.0 56-57 37.932665736091224 41.0 37.0 41.0 29.5 41.0 58-59 37.92890705726664 41.0 37.0 41.0 29.5 41.0 60-61 37.94195438971775 41.0 37.0 41.0 32.0 41.0 62-63 37.93980294762794 41.0 37.0 41.0 32.0 41.0 64-65 37.92213712089625 41.0 37.0 41.0 32.0 41.0 66-67 37.79332904301796 41.0 37.0 41.0 27.0 41.0 68-69 37.7996258361583 41.0 37.0 41.0 27.0 41.0 70-71 37.78699690009608 41.0 37.0 41.0 27.0 41.0 72-73 37.66022841397314 41.0 37.0 41.0 27.0 41.0 74-75 37.55825408335297 41.0 37.0 41.0 27.0 41.0 76-77 36.51068632960499 39.0 34.5 41.0 24.5 41.0 78-79 37.029766147598934 41.0 37.0 41.0 27.0 41.0 80-81 37.68462284502293 41.0 37.0 41.0 27.0 41.0 82-83 37.8742082555336 41.0 37.0 41.0 32.0 41.0 84-85 37.7917877925421 41.0 37.0 41.0 27.0 41.0 86-87 37.64900567409314 41.0 37.0 41.0 27.0 41.0 88-89 37.55707956420064 41.0 37.0 41.0 27.0 41.0 90-91 37.406830484201365 41.0 37.0 41.0 27.0 41.0 92-93 37.296961912876384 41.0 37.0 41.0 27.0 41.0 94-95 37.14356924025162 41.0 37.0 41.0 27.0 41.0 96-97 37.08833475336729 41.0 37.0 41.0 27.0 41.0 98-99 36.96114932110291 41.0 37.0 41.0 27.0 41.0 100 36.84181063393942 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.350815475660605 1101 2 -0.46222427577168723 1101 3 0.02848289224547962 1101 4 0.18748526770768237 1101 5 -1.1132691548577043 1101 6 -0.7889703842469231 1101 7 -0.30725488673937207 1101 8 -0.1320184627449521 1101 9 -0.2001497478510217 1101 10-11 -0.08693342043961394 1101 12-13 -0.02826799333890051 1101 14-15 0.09749024652500538 1101 16-17 0.014907227675301726 1101 18-19 0.036461453115840925 1101 20-21 -0.053555157346963256 1101 22-23 0.06239879570745899 1101 24-25 0.09997805146434047 1101 26-27 0.1664831300923808 1101 28-29 -0.021412541405034347 1101 30-31 -0.05277300409960617 1101 32-33 -0.21138158597208445 1101 34-35 -0.08726638841365997 1101 36-37 0.03880895115904792 1101 38-39 -0.021365071543613112 1101 40-41 0.022348696840730042 1101 42-43 -0.10481190147343256 1101 44-45 -0.08005329006009987 1101 46-47 0.14686383536536596 1101 48-49 0.053219291618241016 1101 50-51 0.06427076959819544 1101 52-53 -0.032166246720272795 1101 54-55 -0.11531182193585465 1101 56-57 0.06622319488854345 1101 58-59 0.10174688744849192 1101 60-61 0.0831089083219183 1101 62-63 -0.05082315220737854 1101 64-65 0.03149667792896338 1101 66-67 -0.007374486127758928 1101 68-69 0.16535101639454552 1101 70-71 0.00679483346750942 1101 72-73 0.13153914944309975 1101 74-75 0.18032238477076845 1101 76-77 -0.583349706058037 1101 78-79 -0.15721921925147342 1101 80-81 -0.024299383567566224 1101 82-83 -0.06788847939605347 1101 84-85 0.16725080324340524 1101 86-87 -0.0773896003245298 1101 88-89 -0.1729136030977898 1101 90-91 -0.045132691731893715 1101 92-93 -0.021751132002108875 1101 94-95 0.003552031588895943 1101 96-97 -0.10810895891160754 1101 98-99 -0.3618209024829184 1101 100 -0.5547721505718286 1102 1 1.705668538323092 1102 2 0.881532557816767 1102 3 0.008536406835951027 1102 4 1.9179393651391763E-4 1102 5 -0.782014750989589 1102 6 -0.07054004432905003 1102 7 -0.06547341578667698 1102 8 -0.21351583864699109 1102 9 -0.2755242284982984 1102 10-11 -0.1523635113111581 1102 12-13 -0.20024435823430053 1102 14-15 -0.18956830366087019 1102 16-17 -0.23913218238860168 1102 18-19 -0.11325259917174435 1102 20-21 -0.0489128729238999 1102 22-23 -0.1673487259777957 1102 24-25 -0.06780476798314083 1102 26-27 -0.08373630402574861 1102 28-29 -0.24667495120166194 1102 30-31 -0.21199637436468777 1102 32-33 -0.2405815094358914 1102 34-35 -0.11072533828853892 1102 36-37 -0.16705366030437574 1102 38-39 -0.15730488737081316 1102 40-41 -0.15048110732380593 1102 42-43 -0.08043876932094207 1102 44-45 -0.15774207933045403 1102 46-47 -0.31024704492230626 1102 48-49 -0.21050710972296116 1102 50-51 -0.14775649793831747 1102 52-53 -0.2099002895319444 1102 54-55 -0.127963831314581 1102 56-57 -0.22240480277892516 1102 58-59 -0.11976160442409878 1102 60-61 -0.037017983822792644 1102 62-63 -0.31909920488371313 1102 64-65 -0.2325091412512421 1102 66-67 -0.11749548620064587 1102 68-69 -0.39207542382219174 1102 70-71 -0.35088733481492085 1102 72-73 -0.18861902535097386 1102 74-75 -0.3068152831836457 1102 76-77 -0.14309045489785177 1102 78-79 -0.3487897601072234 1102 80-81 -0.3368350700850513 1102 82-83 -0.2806909795782886 1102 84-85 -0.18347856843091392 1102 86-87 -0.12146093083938325 1102 88-89 -0.3557644850862758 1102 90-91 -0.38774072317248454 1102 92-93 -0.21646056782104495 1102 94-95 -0.23938507510012386 1102 96-97 -0.12493325368528474 1102 98-99 -0.40272448510555847 1102 100 -0.3215812595933798 1103 1 1.5076328322328632 1103 2 0.705732051075783 1103 3 0.4377074058056927 1103 4 1.061926519304107 1103 5 2.8794420739505497 1103 6 -0.15075293251527455 1103 7 -0.3616209498951548 1103 8 -0.20458219449563586 1103 9 -0.304491708134492 1103 10-11 -0.1983239057525097 1103 12-13 -0.40486833015307155 1103 14-15 -0.31256456403267663 1103 16-17 -0.4012022557093715 1103 18-19 -0.26289074306276206 1103 20-21 -0.18777841880991275 1103 22-23 -0.34073817747262325 1103 24-25 -0.2873817136422119 1103 26-27 -0.23412617173818262 1103 28-29 -0.2530661862601633 1103 30-31 -0.15250020340845083 1103 32-33 -0.4196437062735896 1103 34-35 -0.40353363605346004 1103 36-37 -0.4042628816609266 1103 38-39 -0.18266963168184702 1103 40-41 -0.43651756039013634 1103 42-43 -0.09827282351785271 1103 44-45 -0.35363497688553025 1103 46-47 -0.46684694268786586 1103 48-49 -0.3631703485290032 1103 50-51 -0.24876997990723027 1103 52-53 -0.3311921150497241 1103 54-55 -0.32205733942765846 1103 56-57 -0.30889418922985357 1103 58-59 -0.5622935052092117 1103 60-61 -0.29068125269778733 1103 62-63 -0.22571453276982112 1103 64-65 -0.45264394511463024 1103 66-67 -0.48844903599879075 1103 68-69 -0.450205039999922 1103 70-71 -0.6109054394896916 1103 72-73 -0.16711735019379148 1103 74-75 -0.3548646785603111 1103 76-77 -0.447328119901222 1103 78-79 -0.4722774104717615 1103 80-81 -0.198222736433614 1103 82-83 -0.5127789834904135 1103 84-85 -0.537485098141417 1103 86-87 -0.3142511872325926 1103 88-89 -0.34986859646168256 1103 90-91 -0.6372987886943093 1103 92-93 -0.32334145203816433 1103 94-95 -0.4231768017068802 1103 96-97 -0.26087578083833307 1103 98-99 -0.38485244907345617 1103 100 -0.26839402971939563 1104 1 1.8262457320212668 1104 2 -0.39549474795562745 1104 3 0.15217781822244625 1104 4 0.15815762008472056 1104 5 -1.1769840674565302 1104 6 -0.39596681002644374 1104 7 -0.2899116568045699 1104 8 -0.015268484907537072 1104 9 0.05150992752756167 1104 10-11 -0.08259635371814511 1104 12-13 -0.07072923903734107 1104 14-15 -0.11120700652335813 1104 16-17 -0.08755644583356315 1104 18-19 0.060264320954296124 1104 20-21 0.025734007969795414 1104 22-23 0.14309050005051205 1104 24-25 -0.04565884077714344 1104 26-27 0.03564757042667566 1104 28-29 -0.054284691498111215 1104 30-31 0.004454063669548702 1104 32-33 -0.006603080858575083 1104 34-35 -0.13183736106906707 1104 36-37 -0.16923173531694147 1104 38-39 -0.0849606084020067 1104 40-41 -0.09274034638033868 1104 42-43 -0.05749780526362969 1104 44-45 -0.01963052868263304 1104 46-47 -0.1683541034336571 1104 48-49 -0.24560740099880007 1104 50-51 -0.17387098833803094 1104 52-53 -0.08694409703014117 1104 54-55 -0.0687827345508012 1104 56-57 -0.019009572694443477 1104 58-59 -0.1074991414192894 1104 60-61 -0.0975841559979358 1104 62-63 -0.05018295572599385 1104 64-65 -0.1105680086114944 1104 66-67 -0.5575646814002724 1104 68-69 -0.36025281330010017 1104 70-71 -0.21742504355029268 1104 72-73 -0.20677270633085243 1104 74-75 -0.1369037864210938 1104 76-77 -0.1457857367794091 1104 78-79 -0.28415265292086644 1104 80-81 -0.3963021458315339 1104 82-83 -0.3654166460264605 1104 84-85 -0.17857643537913503 1104 86-87 -0.3032417013375621 1104 88-89 -0.1590491434216048 1104 90-91 -0.30723572961782963 1104 92-93 -0.2694086979055754 1104 94-95 -0.12914896290672573 1104 96-97 -0.16200483908858132 1104 98-99 -0.25468923456708836 1104 100 -0.3286326915000757 1105 1 1.3597560176893833 1105 2 0.26654586476315956 1105 3 -0.07553414191861307 1105 4 -0.17767040994799466 1105 5 -1.1108226634406257 1105 6 0.4096024209435285 1105 7 0.14609568425826325 1105 8 0.06691046780161969 1105 9 0.14369526878811456 1105 10-11 0.013379461123271597 1105 12-13 0.014176014178637786 1105 14-15 0.02754977033452377 1105 16-17 0.05669659299407925 1105 18-19 0.1484486625295176 1105 20-21 -0.11918247665075654 1105 22-23 -0.04203496497630255 1105 24-25 -0.0795495287985375 1105 26-27 -0.0497994299401725 1105 28-29 0.028765222008026115 1105 30-31 0.08078821953514392 1105 32-33 -0.10065128958002134 1105 34-35 -0.07352413937729807 1105 36-37 -0.17962906507308674 1105 38-39 -0.09115067809393196 1105 40-41 -0.10125448374774493 1105 42-43 0.03146440392052341 1105 44-45 0.004275099415345096 1105 46-47 -0.20109906774459319 1105 48-49 -0.08258567139260009 1105 50-51 0.008487024476210081 1105 52-53 -0.13238411164402208 1105 54-55 -0.20266353612581156 1105 56-57 0.029968735839972283 1105 58-59 0.014346542695200526 1105 60-61 -0.14592009644238146 1105 62-63 -0.16003542686082994 1105 64-65 -0.03590827550539899 1105 66-67 5.08154107826897E-4 1105 68-69 -0.15407247949953984 1105 70-71 -0.2401257296295256 1105 72-73 -0.19553954528264939 1105 74-75 -0.2393818216981387 1105 76-77 -0.2736044404304252 1105 78-79 -0.200525109983829 1105 80-81 -0.21780787670841306 1105 82-83 -0.14003678316201018 1105 84-85 -0.3219479745583129 1105 86-87 -0.3301780020099869 1105 88-89 -0.1645454053913582 1105 90-91 -0.15285836747227677 1105 92-93 -0.09699308775876858 1105 94-95 0.0032333143584182267 1105 96-97 -0.17828510629364303 1105 98-99 -0.24378704364023918 1105 100 -0.27455985197717325 1106 1 1.2645411322504643 1106 2 -0.6435269394033316 1106 3 -0.05587958662250969 1106 4 -0.2558418424982456 1106 5 -1.1235847373024939 1106 6 -0.5804501906976789 1106 7 -0.07008917184513308 1106 8 -0.1655383235066381 1106 9 -0.20400625651014792 1106 10-11 -0.26162084972225585 1106 12-13 -0.1478428073106599 1106 14-15 -0.1699415819429717 1106 16-17 -0.21945641594916054 1106 18-19 -0.11832161931204865 1106 20-21 -0.0802455517667866 1106 22-23 -0.1979612970737179 1106 24-25 -0.28009413015779927 1106 26-27 -0.31103865085353277 1106 28-29 -0.16992997281751343 1106 30-31 -0.0566598796237443 1106 32-33 -0.20744628985770674 1106 34-35 -0.1646434899671121 1106 36-37 -0.42160813149207144 1106 38-39 -0.1879069428746334 1106 40-41 -0.19269321775266945 1106 42-43 -0.12219971878636926 1106 44-45 -0.19698635316992608 1106 46-47 -0.3385678899384601 1106 48-49 -0.34260372530067684 1106 50-51 -0.43710622213536965 1106 52-53 -0.23328175062719936 1106 54-55 -0.314128845350794 1106 56-57 -0.2936790654045751 1106 58-59 -0.29120167690597043 1106 60-61 -0.25789900536629773 1106 62-63 -0.5121647187049874 1106 64-65 -0.7400714503406718 1106 66-67 -0.5379436859733389 1106 68-69 -0.5215584520771657 1106 70-71 -0.6857051332537409 1106 72-73 -0.37418279989331893 1106 74-75 -0.6829042568702661 1106 76-77 -0.4980449392090023 1106 78-79 -0.22102632559706592 1106 80-81 -0.24084849302805367 1106 82-83 -0.50848040863211 1106 84-85 -0.61160096319297 1106 86-87 -0.6354211785362551 1106 88-89 -0.5886671838919568 1106 90-91 -0.7583607477200758 1106 92-93 -0.742053752371362 1106 94-95 -0.37628420365197 1106 96-97 -0.48629223833336255 1106 98-99 -0.8338252320491009 1106 100 -0.77715860345576 1107 1 1.3894021270900936 1107 2 -0.6272669799699297 1107 3 -0.2127482116480195 1107 4 -0.08011016514848635 1107 5 -1.2224731125143862 1107 6 -0.15132803691944474 1107 7 -0.352637620610011 1107 8 -0.540030512145492 1107 9 -0.2500239408820164 1107 10-11 -0.06075329092359283 1107 12-13 -0.38849770383250615 1107 14-15 -0.4354553908301426 1107 16-17 -0.4464665242794723 1107 18-19 -0.28203242193929867 1107 20-21 -0.3567935590471123 1107 22-23 -0.18857683483106058 1107 24-25 -0.17015156479934745 1107 26-27 -0.24593592239568807 1107 28-29 -0.056829959906174565 1107 30-31 -0.2642467500396961 1107 32-33 -0.2779888577151368 1107 34-35 -0.30263989978139705 1107 36-37 -0.18263587421486704 1107 38-39 -0.32068842051510416 1107 40-41 -0.40898149883672374 1107 42-43 -0.09020125148746416 1107 44-45 -0.2691732427446496 1107 46-47 -0.302087104928944 1107 48-49 -0.35561877326006197 1107 50-51 -0.3608743344557013 1107 52-53 -0.3175465747205948 1107 54-55 -0.3455608796366505 1107 56-57 -0.33622026715885056 1107 58-59 -0.4129439582149317 1107 60-61 -0.3673717645198167 1107 62-63 -0.2346221216929223 1107 64-65 -0.26160298524919057 1107 66-67 -0.39413217488832686 1107 68-69 -0.4199491159283255 1107 70-71 -0.20756320013448004 1107 72-73 -0.3002083143685894 1107 74-75 -0.4455517481134663 1107 76-77 -0.5200292040331007 1107 78-79 -0.8334424539441159 1107 80-81 -0.5453695447608808 1107 82-83 -0.5877564572620528 1107 84-85 -0.43445861774380745 1107 86-87 -0.4055285092623322 1107 88-89 -0.32520544519250194 1107 90-91 -0.2880526664702643 1107 92-93 -0.42776756209152467 1107 94-95 -0.31242846081256204 1107 96-97 -0.32386590586216357 1107 98-99 -0.5426674179807307 1107 100 -0.5499298353226152 1108 1 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0.06323357056088241 2223 30-31 0.09249492701650297 2223 32-33 0.11023167200716699 2223 34-35 0.21350687863964168 2223 36-37 0.2547051453174589 2223 38-39 0.1142870766396058 2223 40-41 0.2427090123284259 2223 42-43 0.2473339803534813 2223 44-45 0.2718228359771686 2223 46-47 0.27970713700527483 2223 48-49 0.3054708730019726 2223 50-51 0.2004547854580565 2223 52-53 0.33636914865853385 2223 54-55 0.26858905061973104 2223 56-57 0.3996970773992956 2223 58-59 0.407985298305789 2223 60-61 0.3459169658172172 2223 62-63 0.297784999973679 2223 64-65 0.2695598609486538 2223 66-67 0.3637233426751294 2223 68-69 0.24833914122194045 2223 70-71 0.4089946335753467 2223 72-73 0.42738546919166254 2223 74-75 0.2617715372017031 2223 76-77 0.3479327599800186 2223 78-79 0.4818196709524969 2223 80-81 0.2761180721471064 2223 82-83 0.3066060367202468 2223 84-85 0.24721629883130447 2223 86-87 0.21879905119241982 2223 88-89 0.2869900179750502 2223 90-91 0.29487475490873294 2223 92-93 0.34998278456922094 2223 94-95 0.4533407968602603 2223 96-97 0.39986585073055636 2223 98-99 0.3960972765257722 2223 100 0.6269770044892624 2224 1 1.5397076351662733 2224 2 -0.7306923585163094 2224 3 0.13836983462183738 2224 4 -0.363563278817006 2224 5 -1.009469193708668 2224 6 0.5962717669335369 2224 7 0.3838112418282478 2224 8 0.20815359812684164 2224 9 0.27465279279052623 2224 10-11 0.46890154159480346 2224 12-13 0.4674916850438464 2224 14-15 0.34486888021327644 2224 16-17 0.3946040315889334 2224 18-19 0.2735470593626488 2224 20-21 0.25921574222461885 2224 22-23 0.39969296158785284 2224 24-25 0.37706024503727775 2224 26-27 0.2497308162136065 2224 28-29 0.2486898586330284 2224 30-31 0.35089766857706906 2224 32-33 0.4015540502719048 2224 34-35 0.3007516574621931 2224 36-37 0.26496004308253873 2224 38-39 0.260362884499294 2224 40-41 0.2787927203997427 2224 42-43 0.32926600924335503 2224 44-45 0.5227849776098239 2224 46-47 0.3237240895088149 2224 48-49 0.15852010246690895 2224 50-51 0.30689032550790074 2224 52-53 0.2601066403291412 2224 54-55 0.40780094959140456 2224 56-57 0.6320483219470532 2224 58-59 0.46277639832279505 2224 60-61 0.46579271037758474 2224 62-63 0.4816221921522228 2224 64-65 0.33035325048115993 2224 66-67 0.5333403980197318 2224 68-69 0.4419341931347063 2224 70-71 0.5015562304107064 2224 72-73 0.4025411342780103 2224 74-75 0.4420903282333697 2224 76-77 0.5777952344629895 2224 78-79 0.42084321781393896 2224 80-81 0.35175043486364643 2224 82-83 0.39018270904697516 2224 84-85 0.1967680370812701 2224 86-87 0.3447010869410789 2224 88-89 0.420675674168578 2224 90-91 0.3119886798656708 2224 92-93 0.2930155871265754 2224 94-95 0.5065588516507873 2224 96-97 0.5357302892826112 2224 98-99 0.4726909265781316 2224 100 0.7383958613424682 2225 1 1.1504989113913364 2225 2 1.6310143167873488 2225 3 0.8765292280612549 2225 4 0.9125421293930405 2225 5 3.1784576189486984 2225 6 -0.1072618644655492 2225 7 0.24040825364176754 2225 8 0.5165695327829383 2225 9 0.22842823313194316 2225 10-11 0.2692079230725213 2225 12-13 0.3150600975921023 2225 14-15 0.23051140357585354 2225 16-17 0.3190712118745722 2225 18-19 0.22327850022923457 2225 20-21 0.2539627390157406 2225 22-23 0.30838892091576753 2225 24-25 0.29202930447466 2225 26-27 0.21619628822416814 2225 28-29 0.2411622696300455 2225 30-31 0.15564651591756018 2225 32-33 0.19379890434321823 2225 34-35 0.25440047695429513 2225 36-37 0.26394330634687435 2225 38-39 0.2202328444772732 2225 40-41 0.2183281915301123 2225 42-43 0.4731859710981112 2225 44-45 0.4055658697778668 2225 46-47 0.23847522995698966 2225 48-49 0.2863732308050473 2225 50-51 0.2708125624401774 2225 52-53 0.39802469197110213 2225 54-55 0.4822722850078236 2225 56-57 0.4913699357900114 2225 58-59 0.4736511213755463 2225 60-61 0.46394658659009025 2225 62-63 0.3780217508396433 2225 64-65 0.3731322478440049 2225 66-67 0.5908998594164387 2225 68-69 0.5751088392642671 2225 70-71 0.3721511976048433 2225 72-73 0.31777458132697944 2225 74-75 0.39792579554462293 2225 76-77 0.5354783007560897 2225 78-79 0.38830846182501944 2225 80-81 0.4430448048171485 2225 82-83 0.38292450692534175 2225 84-85 0.23885074270574336 2225 86-87 0.3359590032028237 2225 88-89 0.4392627952956403 2225 90-91 0.5315930786329943 2225 92-93 0.4697355940894923 2225 94-95 0.609263931941527 2225 96-97 0.5926056702720501 2225 98-99 0.48654078883595275 2225 100 0.6554764975970784 2226 1 1.409301289956355 2226 2 1.4450731752888348 2226 3 0.7872609811952671 2226 4 0.8891238955058949 2226 5 3.1937729815718576 2226 6 -0.4505920244572934 2226 7 0.19445322056022007 2226 8 0.3230163471195979 2226 9 0.36395018377127286 2226 10-11 0.21765865375554228 2226 12-13 0.23494387940910855 2226 14-15 0.16743274981701717 2226 16-17 0.15486652156857872 2226 18-19 0.1516749765445482 2226 20-21 0.2230532213266727 2226 22-23 0.22400683048927306 2226 24-25 0.2681476589068197 2226 26-27 0.18019180965465864 2226 28-29 0.19080710329797057 2226 30-31 0.23989103229549613 2226 32-33 0.18013799182225654 2226 34-35 0.18639002960920692 2226 36-37 0.2609949478843987 2226 38-39 0.39487991030691205 2226 40-41 0.28292595764560957 2226 42-43 0.35646354318202356 2226 44-45 0.1765332397977275 2226 46-47 0.3767800526355529 2226 48-49 0.33926900301622 2226 50-51 0.3921580084741265 2226 52-53 0.2856712547153961 2226 54-55 0.5195527815134753 2226 56-57 0.5104441096541237 2226 58-59 0.5735601121253637 2226 60-61 0.6313414045624768 2226 62-63 0.5042935239413495 2226 64-65 0.5778624759850075 2226 66-67 0.6106445100553799 2226 68-69 0.584223264926159 2226 70-71 0.6007050102612865 2226 72-73 0.615516461931918 2226 74-75 0.5296259871543825 2226 76-77 0.6234094040701379 2226 78-79 0.37215473404869215 2226 80-81 0.38095650199057474 2226 82-83 0.40220044501719343 2226 84-85 0.3975083247917297 2226 86-87 0.4867978901600267 2226 88-89 0.4739528246475331 2226 90-91 0.3177007998584429 2226 92-93 0.2333625893480331 2226 94-95 0.574513439582141 2226 96-97 0.39617850392804144 2226 98-99 0.5548566986079138 2226 100 0.7514312904831115 2227 1 1.7909995997009514 2227 2 2.049465929817421 2227 3 0.5963973727776555 2227 4 -0.11956920997128861 2227 5 3.222771748973514 2227 6 0.26628102782423824 2227 7 0.530791514790792 2227 8 0.3497015561707215 2227 9 0.2330896015130861 2227 10-11 0.33575981139367883 2227 12-13 0.4128937819343932 2227 14-15 0.31702058425966584 2227 16-17 0.2893655154366499 2227 18-19 0.1667707548208952 2227 20-21 0.18881417653587818 2227 22-23 0.2566888567871288 2227 24-25 0.32823724115475983 2227 26-27 0.3432520827556331 2227 28-29 0.26826480892071203 2227 30-31 0.32581578994670934 2227 32-33 0.18584615112113312 2227 34-35 0.20413675327954905 2227 36-37 0.3130945276909216 2227 38-39 0.24547476867703466 2227 40-41 0.24306555741950575 2227 42-43 0.3653714696384185 2227 44-45 0.22328211712891743 2227 46-47 0.2753078227690082 2227 48-49 0.2902626470903087 2227 50-51 0.24764105586953633 2227 52-53 0.35912012500977397 2227 54-55 0.4045524461912251 2227 56-57 0.48125026068656496 2227 58-59 0.41472143053977817 2227 60-61 0.37245899893752465 2227 62-63 0.38141889713190125 2227 64-65 0.511130952819201 2227 66-67 0.4844991698288368 2227 68-69 0.2942959654331716 2227 70-71 0.4717288399453352 2227 72-73 0.40958903822104276 2227 74-75 0.36488431247907016 2227 76-77 0.6139307690530487 2227 78-79 0.3818552929301191 2227 80-81 0.4218647429561244 2227 82-83 0.31893547007054934 2227 84-85 0.3503777086808668 2227 86-87 0.37603136992290587 2227 88-89 0.4867727889745268 2227 90-91 0.5481605976938084 2227 92-93 0.40899050917066404 2227 94-95 0.6944547400179957 2227 96-97 0.49768046680208755 2227 98-99 0.5575960996992535 2227 100 0.5308077496717516 2228 1 1.2563530972132906 2228 2 1.783352003520518 2228 3 -0.09174467032426392 2228 4 -0.1998792309373041 2228 5 3.294460400379023 2228 6 0.017069389386104916 2228 7 0.5130065511691271 2228 8 0.40471236779864483 2228 9 0.3501631008657071 2228 10-11 0.16487891018369538 2228 12-13 0.2859785792738805 2228 14-15 0.2577375364979062 2228 16-17 0.251791493576782 2228 18-19 0.2738070214244601 2228 20-21 0.24895361038776542 2228 22-23 0.4000173593930967 2228 24-25 0.30053400555328835 2228 26-27 0.29390521681325055 2228 28-29 0.335246951660487 2228 30-31 0.3306470102475956 2228 32-33 0.33777252689257153 2228 34-35 0.3766982218206749 2228 36-37 0.36308959837728594 2228 38-39 0.2783838524162121 2228 40-41 0.37231183250150224 2228 42-43 0.5797847827287015 2228 44-45 0.45656993646628763 2228 46-47 0.4199289557224546 2228 48-49 0.43815273654092124 2228 50-51 0.46037610197504364 2228 52-53 0.4993457889883217 2228 54-55 0.4021010985168729 2228 56-57 0.6334577716460785 2228 58-59 0.5321031763054904 2228 60-61 0.49457272148946174 2228 62-63 0.5951337264790553 2228 64-65 0.47215306243398913 2228 66-67 0.5365239675626654 2228 68-69 0.5225604855480626 2228 70-71 0.4831857150233816 2228 72-73 0.6636489852473488 2228 74-75 0.538472392741447 2228 76-77 0.598652525728987 2228 78-79 0.5610585186729651 2228 80-81 0.5147519076806404 2228 82-83 0.5204484407378445 2228 84-85 0.5746565500949856 2228 86-87 0.5714715904932675 2228 88-89 0.5144498992137017 2228 90-91 0.6362727362929661 2228 92-93 0.5952572770525748 2228 94-95 0.8246045582906376 2228 96-97 0.5949816675828927 2228 98-99 0.688455081894567 2228 100 0.7284157180715667 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 15.0 13 30.0 14 129.0 15 441.0 16 1739.0 17 4239.0 18 7220.0 19 9230.0 20 10896.0 21 12662.0 22 14726.0 23 16553.0 24 18961.0 25 21598.0 26 24456.0 27 27646.0 28 30986.0 29 35188.0 30 40788.0 31 47062.0 32 55116.0 33 65267.0 34 80066.0 35 104444.0 36 146026.0 37 241095.0 38 597437.0 39 1142866.0 40 1268.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.050802389440136 19.684785947010113 19.29378442120037 35.97062724234938 2 18.524510573526282 51.97041450369528 12.200083177028926 17.304991745749504 3 41.31486684915614 15.351268060112758 23.506698330402624 19.82716676032848 4 17.875895074597103 16.189764878632417 22.01410365643638 43.9202363903341 5 17.664376484237625 35.59251672316589 26.269310951181048 20.47379584141544 6 17.525442778674112 27.884596559287928 30.645468883128185 23.944491778909775 7 20.355020575385673 26.368326595725396 36.4604173087033 16.816235520185632 8 21.248373003643746 27.85109584322825 33.957761543063285 16.94276961006472 9 20.891648387506116 28.082845385493897 27.812265467795445 23.213240759204538 10-11 19.68518753512318 30.414063774631543 28.62538295596686 21.275365734278413 12-13 24.4022080017403 26.578630603846783 27.35090549825064 21.668255896162282 14-15 21.52112829251491 27.389572720845496 28.82267099323822 22.266627993401375 16-17 20.954208436814532 29.662418650182186 28.16037923970778 21.222993673295505 18-19 21.34102568750793 29.468448053949203 29.81456773561989 19.37595852292297 20-21 22.86463752877835 28.87930315610101 29.861320087739973 18.394739227380672 22-23 21.915849391802475 30.44644054891866 28.711165092543915 18.926544966734948 24-25 22.226111705309716 28.374490147381398 26.675652158149482 22.7237459891594 26-27 20.267915088011893 26.45682794626833 30.06725522542284 23.20800174029694 28-29 19.764903500324856 28.05484412706516 30.696946932429924 21.483305440180064 30-31 20.230282957705008 29.981311650883946 28.287394465579936 21.501010925831114 32-33 20.611025506226998 29.915359933288617 28.997081377009952 20.47653318347443 34-35 21.339376031035297 29.40276997262658 29.788735202944 19.46911879339412 36-37 21.712270906223374 28.044885158530175 28.96263800010877 21.280205935137683 38-39 21.531863687056855 29.36780579843401 28.920073934311873 20.180256580197266 40-41 21.04496708973477 29.571923631651575 29.985740214480515 19.39736906413314 42-43 20.365625841257582 29.950972527756402 29.44637659878596 20.237025032200055 44-45 20.533926726247667 30.182187335714154 28.916665155992245 20.367220782045937 46-47 19.820531404556853 28.1613125329259 29.0193347780545 22.998821284462746 48-49 20.88568424487428 28.01379547885358 30.334046371662165 20.766473904609974 50-51 21.674401319725177 30.59452894150064 28.24958758588184 19.481482152892337 52-53 20.28218256863038 30.53691153500878 28.169364651420466 21.01154124494037 54-55 22.46508896360317 28.161366890573795 28.345293843433268 21.028250302389765 56-57 21.712011562730936 28.49748316246203 29.406840750649245 20.383664524157798 58-59 21.25074652735599 28.04627581207853 28.93092206054967 21.77205560001581 60-61 19.50953294177811 30.39849406113852 29.586717021739055 20.505255975344312 62-63 20.49499658606789 31.121408708409586 27.490729080283426 20.892865625239093 64-65 19.808596237657255 30.303970217702204 28.7795961675015 21.10783737713905 66-67 20.248699309319655 30.308975218896723 29.367021373021775 20.07530409876185 68-69 20.80969127857441 30.037869586498196 29.331780360023927 19.820658774903468 70-71 19.69156862389645 30.97665101607962 28.972572195130798 20.359208164893136 72-73 20.000072512372423 30.806138172325653 28.56458133168972 20.629207983612204 74-75 19.938600148650362 29.23290974022443 30.45706361147871 20.371426499646503 76-77 19.36011456954843 30.69412468502438 28.621612312600835 21.32414843282635 78-79 19.513786414807026 29.427949893950654 28.58078784692638 22.477475844315936 80-81 20.763564193892705 29.523168478513085 28.41060872818823 21.302658599405977 82-83 20.266718633866905 28.04452259666806 29.895962873665322 21.79279589579972 84-85 21.06168990083933 28.899860413683086 28.20191070101336 21.836538984464223 86-87 21.161702590504504 29.80880300201222 28.645976469735146 20.383517937748127 88-89 20.510849663723874 29.709515436071282 28.201838188640938 21.57779671156391 90-91 20.8706560556895 29.959411199535925 27.537642985334372 21.632289759440205 92-93 20.67915088011892 29.562550985261858 28.876021971248843 20.882276163370374 94-95 21.16735855555354 29.692692565669017 28.368906694704783 20.77104218407266 96-97 20.324220945198775 29.790892446023605 28.784982687671086 21.099903921106538 98-99 22.187172561318274 30.065605568950204 27.61427043489295 20.13295143483857 100 20.674727625401086 31.973496727879198 27.288798651269875 20.062976995449848 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 10.0 2 16.5 3 18.0 4 21.0 5 22.5 6 30.0 7 43.5 8 54.0 9 64.0 10 61.5 11 67.5 12 84.0 13 104.0 14 126.5 15 162.0 16 230.5 17 310.0 18 421.0 19 668.0 20 1119.0 21 1738.5 22 2724.0 23 4206.0 24 6522.5 25 9846.5 26 13975.0 27 18986.0 28 24778.0 29 31709.0 30 39657.0 31 48149.5 32 57545.0 33 68616.5 34 81216.0 35 95274.5 36 109270.5 37 120110.0 38 127446.5 39 132649.0 40 135894.0 41 136544.5 42 134711.0 43 132273.0 44 130223.5 45 125700.0 46 116224.0 47 105934.0 48 100154.0 49 97002.5 50 92822.0 51 85501.0 52 74452.5 53 62386.5 54 51759.5 55 42202.0 56 33193.5 57 24782.5 58 17607.5 59 12569.0 60 9636.0 61 7949.5 62 6420.5 63 4747.0 64 3535.5 65 2931.0 66 2284.5 67 1601.0 68 1306.5 69 1422.0 70 1713.5 71 1630.0 72 1061.5 73 542.5 74 260.5 75 151.0 76 121.0 77 88.5 78 68.5 79 63.5 80 54.5 81 36.5 82 26.5 83 27.0 84 22.5 85 19.5 86 15.5 87 17.5 88 22.0 89 18.5 90 13.5 91 17.0 92 15.0 93 12.5 94 16.5 95 17.0 96 14.5 97 18.5 98 32.0 99 83.0 100 94.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.344234359987674 2 0.006417344959483712 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.175371172706343E-4 30-31 0.010224244511719813 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.42660841506082E-4 40-41 0.001595272193317985 42-43 9.064046552943096E-5 44-45 0.0 46-47 0.00228413973134166 48-49 0.0 50-51 0.0 52-53 0.0015227598208944401 54-55 0.019342675343980566 56-57 0.011747004332614252 58-59 0.013813606946685278 60-61 1.2689665174120334E-4 62-63 0.012073310008520204 64-65 5.4384279317658575E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.175371172706343E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2758150.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.70502084978301 #Duplication Level Percentage of deduplicated Percentage of total 1 85.64749408381964 63.12650337179593 2 8.847257455701723 13.041745904717871 3 2.5371822125796433 5.610092036336435 4 1.056563612410921 3.1149617232747593 5 0.5286801557865921 1.9483190952558647 6 0.3497368578515836 1.5466417439933136 7 0.22308997043037548 1.1509995645363809 8 0.15599873629198144 0.9198312088752233 9 0.11309195462933418 0.7501900386508009 >10 0.5089094398566175 6.22612153117683 >50 0.022547801181852546 1.1254642477809145 >100 0.008947666327794122 1.1723258620954171 >500 4.000425055406167E-4 0.19062143792491942 >1k 1.0001062635838794E-4 0.07618223358526494 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.625618621177238E-5 0.0 2 0.0 0.0 0.0 1.0876855863531715E-4 0.0 3 0.0 0.0 0.0 1.0876855863531715E-4 0.0 4 0.0 0.0 0.0 1.0876855863531715E-4 0.0 5 0.0 0.0 0.0 1.0876855863531715E-4 0.0 6 0.0 0.0 0.0 1.0876855863531715E-4 0.0 7 0.0 0.0 0.0 1.0876855863531715E-4 0.0 8 0.0 0.0 0.0 1.0876855863531715E-4 0.0 9 0.0 0.0 0.0 1.4502474484708952E-4 0.0 10-11 1.812809310588619E-5 0.0 0.0 1.4502474484708952E-4 0.0 12-13 3.625618621177238E-5 0.0 0.0 1.4502474484708952E-4 0.0 14-15 3.625618621177238E-5 0.0 0.0 1.4502474484708952E-4 0.0 16-17 3.625618621177238E-5 0.0 0.0 1.4502474484708952E-4 0.0 18-19 3.625618621177238E-5 0.0 0.0 1.4502474484708952E-4 0.0 20-21 3.625618621177238E-5 0.0 0.0 2.175371172706343E-4 0.0 22-23 3.625618621177238E-5 1.812809310588619E-5 0.0 2.175371172706343E-4 0.0 24-25 3.625618621177238E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 26-27 3.625618621177238E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 28-29 3.625618621177238E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 30-31 3.625618621177238E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 32-33 3.625618621177238E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 34-35 3.625618621177238E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 36-37 3.625618621177238E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 38-39 3.625618621177238E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 40-41 3.625618621177238E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 42-43 5.4384279317658575E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 44-45 7.251237242354476E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 46-47 7.251237242354476E-5 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 48-49 1.2689665174120334E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 50-51 1.4502474484708952E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 52-53 1.6315283795297574E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 54-55 1.8128093105886192E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 56-57 1.994090241647481E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 58-59 2.5379330348240667E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 60-61 3.6256186211772385E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 62-63 3.6256186211772385E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 64-65 3.988180483294962E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 66-67 4.7133042075304097E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 68-69 5.075866069648133E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 70-71 5.800989793883582E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 72-73 6.707394449177891E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 74-75 7.432518173413338E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 76-77 8.701484690825372E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 78-79 9.970451208237406E-4 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 80-81 0.0013958631691532368 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 82-83 0.0016315283795297572 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 84-85 0.001794681217482733 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 86-87 0.0018128093105886191 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 88 0.0018128093105886191 3.625618621177238E-5 0.0 2.175371172706343E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 10665 0.0 80.366035 8 CACCGGC 10390 0.0 79.19523 9 CTTCACC 12105 0.0 74.25687 6 GTGTGGC 4665 0.0 71.6409 7 CCTTCAC 13390 0.0 71.05683 5 CGTAGTG 2590 0.0 70.68015 4 GGCAGCC 30 7.612456E-5 70.58072 1 AGGCAGC 40 9.838732E-8 70.413414 2 TTCACCG 13395 0.0 66.159454 7 GCGTAGT 1870 0.0 63.254528 3 TGTGGCA 5430 0.0 61.893612 8 CAGCGTA 1335 0.0 61.857258 1 CGGGGCA 1125 0.0 60.49899 94 ACCTTCA 5355 0.0 60.13084 4 AGCTGCC 45 7.982308E-6 58.817265 1 GCCTTCA 4025 0.0 58.07592 4 AGCGTAG 1630 0.0 57.3099 2 GGGCCCG 805 0.0 57.14298 8 TGTGTGG 5350 0.0 57.11627 6 GGCCCGA 800 0.0 56.91338 9 >>END_MODULE