FastQCFastQC Report
Tue 7 Mar 2017
1HL_2A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_2A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2758150
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG106650.080.3660358
CACCGGC103900.079.195239
CTTCACC121050.074.256876
GTGTGGC46650.071.64097
CCTTCAC133900.071.056835
CGTAGTG25900.070.680154
GGCAGCC307.612456E-570.580721
AGGCAGC409.838732E-870.4134142
TTCACCG133950.066.1594547
GCGTAGT18700.063.2545283
TGTGGCA54300.061.8936128
CAGCGTA13350.061.8572581
CGGGGCA11250.060.4989994
ACCTTCA53550.060.130844
AGCTGCC457.982308E-658.8172651
GCCTTCA40250.058.075924
AGCGTAG16300.057.30992
GGGCCCG8050.057.142988
TGTGTGG53500.057.116276
GGCCCGA8000.056.913389
Produced by FastQC (version 0.11.5)