FastQCFastQC Report
Tue 7 Mar 2017
1HL_31A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_31A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2453753
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC650.081.428981
TCACCGG91400.077.8527458
CACCGGC92600.074.309439
CTTCACC100000.072.987976
TGCAGCG307.617044E-570.5717851
CGTAGTG19150.069.3647164
CCTTCAC113800.068.6740955
GTGTGGC36900.068.561987
CGGGGCA10650.066.5500394
TTCACCG110150.066.347577
GGCGCGC4450.065.396216
CAGCGTA9900.062.5522581
AGGCGCG4700.061.9176945
ACCTTCA41750.060.2600174
CGGGCCC5950.059.9539157
GCGCGCA4950.058.7905357
GCCTTCA34400.058.535564
TGTGGCA42650.057.1175088
GCGTAGT14000.056.9956673
TGTGTGG41350.056.6429636
Produced by FastQC (version 0.11.5)