##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_31A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2453753 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.804576499753642 12.0 12.0 12.0 2.0 32.0 2 14.547067695892782 12.0 12.0 12.0 12.0 22.0 3 13.69939476385765 12.0 12.0 12.0 12.0 22.0 4 13.759773701754007 12.0 12.0 12.0 12.0 22.0 5 22.441963799942375 22.0 22.0 22.0 22.0 27.0 6 26.812096409051765 27.0 22.0 32.0 22.0 32.0 7 34.96695144132274 37.0 37.0 37.0 27.0 41.0 8 37.62211110898285 41.0 37.0 41.0 32.0 41.0 9 38.56604597121226 41.0 41.0 41.0 32.0 41.0 10-11 38.9222148684077 41.0 41.0 41.0 37.0 41.0 12-13 39.107991920947214 41.0 41.0 41.0 37.0 41.0 14-15 39.06307093664277 41.0 41.0 41.0 37.0 41.0 16-17 38.96882999226084 41.0 41.0 41.0 37.0 41.0 18-19 38.89218576604899 41.0 41.0 41.0 37.0 41.0 20-21 38.90878462502134 41.0 41.0 41.0 37.0 41.0 22-23 38.89893481536242 41.0 41.0 41.0 37.0 41.0 24-25 38.91942322638016 41.0 41.0 41.0 37.0 41.0 26-27 38.89107481478372 41.0 41.0 41.0 37.0 41.0 28-29 38.83759367792928 41.0 41.0 41.0 37.0 41.0 30-31 38.71163377079927 41.0 41.0 41.0 32.0 41.0 32-33 38.75091095150979 41.0 41.0 41.0 32.0 41.0 34-35 38.721658618451 41.0 41.0 41.0 32.0 41.0 36-37 38.649694569909855 41.0 41.0 41.0 32.0 41.0 38-39 38.551193416778304 41.0 41.0 41.0 32.0 41.0 40-41 38.60576125632857 41.0 41.0 41.0 32.0 41.0 42-43 38.343913384925045 41.0 41.0 41.0 32.0 41.0 44-45 38.452631540338416 41.0 41.0 41.0 32.0 41.0 46-47 38.47010986843419 41.0 41.0 41.0 32.0 41.0 48-49 38.411813250966986 41.0 41.0 41.0 32.0 41.0 50-51 38.29013311445773 41.0 41.0 41.0 32.0 41.0 52-53 38.255004476815714 41.0 41.0 41.0 32.0 41.0 54-55 38.12954298986084 41.0 37.0 41.0 32.0 41.0 56-57 37.9297125668313 41.0 37.0 41.0 29.5 41.0 58-59 37.93327649522996 41.0 37.0 41.0 29.5 41.0 60-61 37.92982056466156 41.0 37.0 41.0 29.5 41.0 62-63 37.93082249924911 41.0 37.0 41.0 32.0 41.0 64-65 37.91396526056208 41.0 37.0 41.0 32.0 41.0 66-67 37.76649850249801 41.0 37.0 41.0 27.0 41.0 68-69 37.799672786951255 41.0 37.0 41.0 27.0 41.0 70-71 37.77212294799028 41.0 37.0 41.0 27.0 41.0 72-73 37.621407696699706 41.0 37.0 41.0 27.0 41.0 74-75 37.54985607760847 41.0 37.0 41.0 27.0 41.0 76-77 36.47866064758759 39.0 34.5 41.0 24.5 41.0 78-79 37.02092101364725 41.0 37.0 41.0 27.0 41.0 80-81 37.66696872097558 41.0 37.0 41.0 27.0 41.0 82-83 37.84864674643292 41.0 37.0 41.0 32.0 41.0 84-85 37.77249401223351 41.0 37.0 41.0 27.0 41.0 86-87 37.63073239238016 41.0 37.0 41.0 27.0 41.0 88-89 37.54888124436323 41.0 37.0 41.0 27.0 41.0 90-91 37.38341980631302 41.0 37.0 41.0 27.0 41.0 92-93 37.27426945580912 41.0 37.0 41.0 27.0 41.0 94-95 37.11077765365951 41.0 37.0 41.0 27.0 41.0 96-97 37.05359280253555 41.0 37.0 41.0 27.0 41.0 98-99 36.93290278198336 41.0 37.0 41.0 27.0 41.0 100 36.793381403914736 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4139565933866827 1101 2 -0.519336403710227 1101 3 -0.02303172695281397 1101 4 0.17955688579712437 1101 5 -1.2203262395037626 1101 6 -0.7431023075646799 1101 7 -0.25557518607217133 1101 8 -0.1269987787775051 1101 9 -0.19705762039280472 1101 10-11 -0.06164395753904017 1101 12-13 -0.10888938024726968 1101 14-15 -0.07131608822236757 1101 16-17 -0.08485955804469825 1101 18-19 -0.03239298045529182 1101 20-21 -0.08409081606486524 1101 22-23 0.08871781261643719 1101 24-25 -0.20656815900133552 1101 26-27 0.025517726839943577 1101 28-29 -0.09095909246946121 1101 30-31 0.08179570247794032 1101 32-33 -0.11868548377911026 1101 34-35 -0.019624890972977482 1101 36-37 0.054044216068440676 1101 38-39 0.00895308231006453 1101 40-41 -0.012539965147865928 1101 42-43 0.1355392583585413 1101 44-45 -0.18250960566796692 1101 46-47 -0.17546802055330346 1101 48-49 -0.11600361855837349 1101 50-51 -0.07644960295895231 1101 52-53 -0.14315424792731335 1101 54-55 0.019914431837491975 1101 56-57 0.20723919157935455 1101 58-59 -0.12121142964598874 1101 60-61 0.00738600438989323 1101 62-63 -0.07915053072635914 1101 64-65 -0.028777860587098303 1101 66-67 0.015422725034312634 1101 68-69 -0.04609314693168898 1101 70-71 -0.04578203669460379 1101 72-73 -0.18172441956122043 1101 74-75 -0.10212315370731062 1101 76-77 -0.5171701641216657 1101 78-79 -0.3333441842087197 1101 80-81 -0.04785087568286173 1101 82-83 -0.08641099171332911 1101 84-85 0.002048950877529876 1101 86-87 -0.17822601223414836 1101 88-89 -0.32706755588798586 1101 90-91 -0.11483842930780241 1101 92-93 0.11018981142247242 1101 94-95 -0.12848181380531543 1101 96-97 -0.39808438594317863 1101 98-99 -0.4893258571827559 1101 100 -0.6757175048349282 1102 1 1.4936216991054287 1102 2 0.6320440103351501 1102 3 0.09887042210982955 1102 4 -0.06038446040220258 1102 5 -0.7878332953037095 1102 6 -0.23332635877638452 1102 7 -0.06591023802493368 1102 8 -0.21981810299742222 1102 9 -0.24551827346818555 1102 10-11 -0.44929985524176885 1102 12-13 -0.3256949555117643 1102 14-15 -0.2490159762756008 1102 16-17 -0.41424479683819726 1102 18-19 -0.12080286330536438 1102 20-21 -0.23217337286602202 1102 22-23 -0.32994197957251714 1102 24-25 -0.1778227511839816 1102 26-27 -0.1298599752555134 1102 28-29 -0.2908878249482143 1102 30-31 -0.4425125137843011 1102 32-33 -0.46596454657967 1102 34-35 -0.2807859839518727 1102 36-37 -0.39370786125366664 1102 38-39 -0.5125744392515088 1102 40-41 -0.3092149884044204 1102 42-43 -0.33135477062148055 1102 44-45 -0.25424730334861323 1102 46-47 -0.5768066516492709 1102 48-49 -0.527445839834563 1102 50-51 -0.23110201612136905 1102 52-53 -0.2497017149345453 1102 54-55 -0.29788384323622097 1102 56-57 -0.1372055682081026 1102 58-59 -0.30751923596231023 1102 60-61 -0.18253784027213982 1102 62-63 -0.27776112940507147 1102 64-65 -0.45126150216822225 1102 66-67 -0.19413122227187074 1102 68-69 -0.37566065398748805 1102 70-71 -0.4192181718506163 1102 72-73 -0.3459011377864698 1102 74-75 -0.3720053730914401 1102 76-77 -0.3240613550145639 1102 78-79 -0.30057563021411937 1102 80-81 -0.5096555837540677 1102 82-83 -0.34090194925239103 1102 84-85 -0.4989863202386289 1102 86-87 -0.49821939003084736 1102 88-89 -0.3550684388484342 1102 90-91 -0.49359377043010966 1102 92-93 -0.501029462342558 1102 94-95 -0.280434575421765 1102 96-97 -0.10956602509617142 1102 98-99 -0.30474297715500853 1102 100 -0.512727422372734 1103 1 1.4628637290338595 1103 2 0.6648188964769854 1103 3 0.39085155418529993 1103 4 1.0730139347398477 1103 5 2.9154848569409246 1103 6 -0.3005276166380213 1103 7 -0.49586903221274525 1103 8 -0.31560318215596084 1103 9 -0.2005139360142678 1103 10-11 -0.3077389140142728 1103 12-13 -0.325771945318742 1103 14-15 -0.33145037228513985 1103 16-17 -0.26994854873583307 1103 18-19 -0.2986677516159233 1103 20-21 -0.13650160969100966 1103 22-23 -0.1004212682300718 1103 24-25 -0.1690318164626632 1103 26-27 -0.2071637054658737 1103 28-29 -0.1251027457896825 1103 30-31 -0.15369230124905897 1103 32-33 -0.2451886845107012 1103 34-35 -0.3911736438954847 1103 36-37 -0.21518422212982813 1103 38-39 -0.07746979817581945 1103 40-41 -0.16519199989866706 1103 42-43 -0.09109723429530447 1103 44-45 -0.44200158658435384 1103 46-47 -0.43323421616889846 1103 48-49 -0.35035572922949143 1103 50-51 -0.27258650972362375 1103 52-53 -0.1322906327397959 1103 54-55 -0.2093368902975925 1103 56-57 -0.28769841140914565 1103 58-59 -0.45606618890092676 1103 60-61 -0.2316529996735639 1103 62-63 -0.452992098826023 1103 64-65 -0.556998327151959 1103 66-67 -0.5136947659717208 1103 68-69 -0.5610390968415331 1103 70-71 -0.38587562142771503 1103 72-73 -0.29057474437939845 1103 74-75 -0.3496501356158461 1103 76-77 -0.4793053367972533 1103 78-79 -0.5325502561087134 1103 80-81 -0.32672238054435354 1103 82-83 -0.2828022962487111 1103 84-85 -0.29033960585996965 1103 86-87 -0.09436339350080658 1103 88-89 -0.3506496576729816 1103 90-91 -0.372665555292393 1103 92-93 -0.32369103250087505 1103 94-95 -0.3348425129624317 1103 96-97 -0.37104672342624667 1103 98-99 -0.23294456032568434 1103 100 -0.12741837878132145 1104 1 1.5595334398538583 1104 2 -0.5811293936992854 1104 3 -0.019795626986974568 1104 4 0.11886412743912445 1104 5 -1.1887213181750909 1104 6 -0.5046680293028629 1104 7 -0.2718406123468 1104 8 -0.2588768413447866 1104 9 0.015878392719336887 1104 10-11 0.02689069700411295 1104 12-13 -0.05012218608359831 1104 14-15 -0.09864058962894973 1104 16-17 -6.987213349560761E-4 1104 18-19 -0.182082723606797 1104 20-21 -0.12570837470276075 1104 22-23 0.041381802723464034 1104 24-25 -0.030383376846138788 1104 26-27 -0.14850196042318942 1104 28-29 -0.04956878090376904 1104 30-31 -0.10603702758113087 1104 32-33 -0.059448758619090825 1104 34-35 -0.13019732274749884 1104 36-37 -0.09386768407500057 1104 38-39 -0.07414966831137093 1104 40-41 -0.08161163862030207 1104 42-43 -0.14606666895670628 1104 44-45 -0.06759628888363522 1104 46-47 -0.31702748611392906 1104 48-49 -0.27652667227614813 1104 50-51 -0.15836761907718255 1104 52-53 -0.13739312166286766 1104 54-55 -0.07750393129316535 1104 56-57 -0.15761667807697677 1104 58-59 -0.08910340913741521 1104 60-61 -0.020341421112561875 1104 62-63 -0.30268710087973005 1104 64-65 -0.38995023098584625 1104 66-67 -0.640816096601732 1104 68-69 -0.5218018538831402 1104 70-71 -0.5365285486214759 1104 72-73 -0.28471817206691696 1104 74-75 -0.397006091814994 1104 76-77 -0.41718844130930677 1104 78-79 -0.19100957952014852 1104 80-81 -0.23003038477412474 1104 82-83 -0.44655069042141093 1104 84-85 -0.3659087460104615 1104 86-87 -0.2973242331627759 1104 88-89 -0.11212444072243954 1104 90-91 -0.19497584919156452 1104 92-93 -0.3065638466094356 1104 94-95 -0.08053998820865615 1104 96-97 -0.2782461824699993 1104 98-99 -0.21245780894707167 1104 100 -0.11596391436295761 1105 1 1.743843540780528 1105 2 0.24518326463576479 1105 3 -0.012641993240057658 1105 4 -0.2556414594421863 1105 5 -1.1111772666588884 1105 6 0.5084644613891527 1105 7 0.09255892468490856 1105 8 -0.29732621124578174 1105 9 -0.20641601872647897 1105 10-11 -0.1867664492585348 1105 12-13 -0.18874417582259895 1105 14-15 -0.12210904599356098 1105 16-17 -0.14567366479699473 1105 18-19 -0.032085912525673166 1105 20-21 -0.1864148300155577 1105 22-23 -0.1951655077632779 1105 24-25 -0.2421812265738268 1105 26-27 -0.19018949446881095 1105 28-29 -0.2003774593635157 1105 30-31 -0.13331451684597795 1105 32-33 -0.18003631430754297 1105 34-35 -0.07324751842165256 1105 36-37 -0.3056208889384706 1105 38-39 -0.08589168582009421 1105 40-41 -0.08133195475194555 1105 42-43 0.017474787511417844 1105 44-45 0.08764937810749984 1105 46-47 -0.23342427962986534 1105 48-49 -0.1597646691332315 1105 50-51 -0.1263994915371569 1105 52-53 -0.27269748567221086 1105 54-55 -0.19483669863696207 1105 56-57 -0.18272747993846394 1105 58-59 -0.14234967627262307 1105 60-61 -0.28138893391267317 1105 62-63 -0.43936928695158173 1105 64-65 -0.3381362589207697 1105 66-67 -0.35666774182247707 1105 68-69 -0.24674547275192538 1105 70-71 -0.39453069617536585 1105 72-73 -0.4167747528976449 1105 74-75 -0.3878331928158971 1105 76-77 -0.2642855318651911 1105 78-79 -0.3179041894108181 1105 80-81 -0.6698257921036159 1105 82-83 -0.32025225152789005 1105 84-85 -0.31581982222353133 1105 86-87 -0.39825623391138976 1105 88-89 -0.1325132378936047 1105 90-91 -0.11437942726309558 1105 92-93 -0.1707261489879528 1105 94-95 -0.14293165645235462 1105 96-97 -0.19322239104411665 1105 98-99 -0.263483020226019 1105 100 -0.298971681732894 1106 1 1.2950333667766518 1106 2 -0.7326420918759347 1106 3 -0.040595999233318025 1106 4 -0.28499929190089723 1106 5 -1.1973755343038057 1106 6 -0.7174232615669389 1106 7 -0.1887714293244045 1106 8 -0.12257016762747952 1106 9 -0.2789429686642819 1106 10-11 -0.17631240692360706 1106 12-13 -0.2676090076853015 1106 14-15 -0.2854361562500074 1106 16-17 -0.2463271917033225 1106 18-19 -0.14230134589479349 1106 20-21 -0.24428072305477855 1106 22-23 -0.17420405276774886 1106 24-25 -0.3780348714348065 1106 26-27 -0.41699624612651576 1106 28-29 -0.41413392039175534 1106 30-31 -0.2422925685591153 1106 32-33 -0.30253689058246636 1106 34-35 -0.41006608631099084 1106 36-37 -0.46970961658575305 1106 38-39 -0.2508288355124435 1106 40-41 -0.3302333982427399 1106 42-43 -0.2444028096194586 1106 44-45 -0.25506723376978613 1106 46-47 -0.4227598709917615 1106 48-49 -0.25357755318672304 1106 50-51 -0.34491774854954116 1106 52-53 -0.39804652137218 1106 54-55 -0.46976458639729657 1106 56-57 -0.40684297581525186 1106 58-59 -0.3773772232520045 1106 60-61 -0.3383513732480523 1106 62-63 -0.48078019484935197 1106 64-65 -0.6108931996589462 1106 66-67 -0.6559065397649206 1106 68-69 -0.5081646457818394 1106 70-71 -0.7960084519635515 1106 72-73 -0.6304976757640333 1106 74-75 -0.6166286640218743 1106 76-77 -0.5659415694611596 1106 78-79 -0.460317088831637 1106 80-81 -0.496238184817841 1106 82-83 -0.4377308255226282 1106 84-85 -0.6501962304629103 1106 86-87 -0.460436712832184 1106 88-89 -0.625910892506603 1106 90-91 -0.664068238807694 1106 92-93 -0.634069041908802 1106 94-95 -0.45230749360848677 1106 96-97 -0.5260989269984861 1106 98-99 -0.8315873976310613 1106 100 -0.7844439468152018 1107 1 1.2246561818640487 1107 2 -0.45703644943496435 1107 3 -0.16177772603831997 1107 4 -0.25237041187091336 1107 5 -1.2462464498375532 1107 6 -0.23165969210971937 1107 7 -0.31521739000449855 1107 8 -0.3146073750280749 1107 9 -0.3408790652944944 1107 10-11 -0.19160509151252114 1107 12-13 -0.3158881228170003 1107 14-15 -0.37918360079667224 1107 16-17 -0.3145720637602949 1107 18-19 -0.21481467684304079 1107 20-21 -0.26403480326193574 1107 22-23 -0.21507448276536678 1107 24-25 -0.293525177748478 1107 26-27 -0.38056080539600146 1107 28-29 -0.2756355899546037 1107 30-31 -0.36048824813934033 1107 32-33 -0.3185683141403288 1107 34-35 -0.35431567743845704 1107 36-37 -0.19667880633667778 1107 38-39 -0.24335030132047564 1107 40-41 -0.3334790369357066 1107 42-43 -0.3130661371604333 1107 44-45 -0.21477412327198664 1107 46-47 -0.41594298625974346 1107 48-49 -0.5101565678039179 1107 50-51 -0.5283386061596786 1107 52-53 -0.4779536306433627 1107 54-55 -0.5597037666078677 1107 56-57 -0.4040462450918838 1107 58-59 -0.5383382014795473 1107 60-61 -0.2631301135799262 1107 62-63 -0.4988035942837499 1107 64-65 -0.4195900462295299 1107 66-67 -0.3519983997942262 1107 68-69 -0.5351306412160994 1107 70-71 -0.3322417989259634 1107 72-73 -0.41450905604955324 1107 74-75 -0.526248668110604 1107 76-77 -0.5216140370068985 1107 78-79 -0.7953297809165392 1107 80-81 -0.7089419822214964 1107 82-83 -0.558304567387772 1107 84-85 -0.321650751911676 1107 86-87 -0.26854665695294244 1107 88-89 -0.33084270449338504 1107 90-91 -0.273997665704691 1107 92-93 -0.25353904088662205 1107 94-95 -0.18287710415739156 1107 96-97 -0.4096298820565778 1107 98-99 -0.3468247140643257 1107 100 -0.4616514325898464 1108 1 1.6480376443698699 1108 2 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20-21 0.2237075305602758 2223 22-23 0.27991984873044373 2223 24-25 0.2176006841170306 2223 26-27 0.3019350011752522 2223 28-29 0.26059795241799577 2223 30-31 0.252471764672741 2223 32-33 0.20316702782429985 2223 34-35 0.24821207505230802 2223 36-37 0.20620654658565485 2223 38-39 0.17369377393365681 2223 40-41 0.22868601557697588 2223 42-43 0.3485549286026455 2223 44-45 0.4825372356517974 2223 46-47 0.39303885345434253 2223 48-49 0.32049571055546267 2223 50-51 0.2905542654328954 2223 52-53 0.5190900020881415 2223 54-55 0.5561044061529685 2223 56-57 0.5761389611658103 2223 58-59 0.6789021641705801 2223 60-61 0.4564014656779847 2223 62-63 0.5073985711270055 2223 64-65 0.4512714188996867 2223 66-67 0.4605296266391292 2223 68-69 0.5897016216339139 2223 70-71 0.44929594614728074 2223 72-73 0.5840866721319742 2223 74-75 0.32455482655383605 2223 76-77 0.5094147016771586 2223 78-79 0.6236913168767444 2223 80-81 0.39829737194833115 2223 82-83 0.32324529437659066 2223 84-85 0.567271115449536 2223 86-87 0.6207747451273349 2223 88-89 0.43093651042931924 2223 90-91 0.4988745379737196 2223 92-93 0.6621553403403055 2223 94-95 0.7103883335607009 2223 96-97 0.6967314861391145 2223 98-99 0.6615360135271686 2223 100 0.7920781902965786 2224 1 1.2365120870168074 2224 2 -0.921582733064696 2224 3 -0.059542710585109404 2224 4 -0.3501119632718357 2224 5 -0.9993212585724152 2224 6 0.5649773347940652 2224 7 0.21261422067595248 2224 8 0.35496246841317713 2224 9 0.31397792918288303 2224 10-11 0.26932995434044216 2224 12-13 0.1558283867842576 2224 14-15 0.020338553026100215 2224 16-17 0.19704032667391402 2224 18-19 0.10641037002325504 2224 20-21 0.1336655818016439 2224 22-23 0.10310108849672872 2224 24-25 0.17247396092508183 2224 26-27 0.15090452621468842 2224 28-29 0.24803885398318215 2224 30-31 0.09624403133575044 2224 32-33 0.14541209068309513 2224 34-35 0.25705668158266803 2224 36-37 -0.03000485639494599 2224 38-39 0.13852954364076453 2224 40-41 0.18622158879261974 2224 42-43 0.31337658463112916 2224 44-45 0.3937625084349605 2224 46-47 0.2514383913559044 2224 48-49 0.10227883271287652 2224 50-51 0.21022823530558554 2224 52-53 0.24803151147550295 2224 54-55 0.13076970960432277 2224 56-57 0.4266115929787446 2224 58-59 0.4258062353649592 2224 60-61 0.33560861317692314 2224 62-63 0.23342996451444975 2224 64-65 0.19634797626629563 2224 66-67 0.32396805914076765 2224 68-69 0.38546392736068213 2224 70-71 0.41049329252663114 2224 72-73 0.34782057864669724 2224 74-75 0.3359739341085799 2224 76-77 0.46082535347797204 2224 78-79 0.2094575474514997 2224 80-81 0.1857162105129646 2224 82-83 0.15595513520127469 2224 84-85 0.11439544305208926 2224 86-87 0.2906489528155092 2224 88-89 0.2950031054067992 2224 90-91 0.32059086092042577 2224 92-93 0.34648755890057714 2224 94-95 0.4581568675708283 2224 96-97 0.24372422444390907 2224 98-99 0.3525647574865687 2224 100 0.4479707237662325 2225 1 0.9647563184138885 2225 2 1.8269492886927843 2225 3 0.8500766123692731 2225 4 0.8376028681911247 2225 5 3.196047545341056 2225 6 -0.2062955592012088 2225 7 0.3156123188577524 2225 8 0.47468610598138383 2225 9 0.38107996132429633 2225 10-11 0.17999296307370116 2225 12-13 0.20200886212879965 2225 14-15 0.12872838777359874 2225 16-17 0.13415610512320342 2225 18-19 -0.011686147198240349 2225 20-21 0.05506847730583786 2225 22-23 0.15088024927663923 2225 24-25 0.08833350159541453 2225 26-27 0.20202603440237965 2225 28-29 0.1609165687129348 2225 30-31 0.10181177377606332 2225 32-33 0.16720890310877934 2225 34-35 0.13715153795627089 2225 36-37 0.08285652382735265 2225 38-39 0.06308840297325702 2225 40-41 0.09828091771625935 2225 42-43 0.3283000355666843 2225 44-45 0.2110306357428371 2225 46-47 0.2507994360669983 2225 48-49 0.24675879658186517 2225 50-51 0.1986107060337119 2225 52-53 0.1663362469675178 2225 54-55 0.3039423670086805 2225 56-57 0.35788679912666055 2225 58-59 0.41354030709274525 2225 60-61 0.28184525266023286 2225 62-63 0.2700294313910021 2225 64-65 0.32905931148047785 2225 66-67 0.4302610786191039 2225 68-69 0.50026147598075 2225 70-71 0.24720218192334187 2225 72-73 0.37448266666997654 2225 74-75 0.4317464514969416 2225 76-77 0.6095512515178569 2225 78-79 0.5451751584789335 2225 80-81 0.2831403396315295 2225 82-83 0.13958575864604938 2225 84-85 0.11347047821694645 2225 86-87 0.3515439758965684 2225 88-89 0.4102264512577207 2225 90-91 0.32227007734559265 2225 92-93 0.34308387925700856 2225 94-95 0.4905430900468204 2225 96-97 0.3954115043457591 2225 98-99 0.4205255447060523 2225 100 0.765678554429492 2226 1 1.3576543537104833 2226 2 1.3810196139410245 2226 3 0.7851592703730823 2226 4 0.9442153420731589 2226 5 3.121893742457118 2226 6 -0.4350874800439044 2226 7 0.23907284630127634 2226 8 0.2288011157295955 2226 9 0.1802809658518285 2226 10-11 0.1424865870032832 2226 12-13 0.22406507306627077 2226 14-15 0.18188307602640208 2226 16-17 -4.8216261600941834E-4 2226 18-19 0.12101163164094686 2226 20-21 0.12439789360311693 2226 22-23 0.3051287300452543 2226 24-25 0.3321840781253087 2226 26-27 0.07034353816145256 2226 28-29 0.20553866016388866 2226 30-31 0.31703223395152236 2226 32-33 0.24985244894450886 2226 34-35 0.13910170017681622 2226 36-37 0.3039436968795215 2226 38-39 0.17643184371303988 2226 40-41 0.21570379534813355 2226 42-43 0.2653379524868029 2226 44-45 0.18298892771318975 2226 46-47 0.20899311300222223 2226 48-49 0.30936151060355854 2226 50-51 0.10009552952993772 2226 52-53 0.32480510386811545 2226 54-55 0.3898188821835191 2226 56-57 0.3911273448600383 2226 58-59 0.4163502506837702 2226 60-61 0.27134793164994875 2226 62-63 0.342314203129753 2226 64-65 0.333021420355891 2226 66-67 0.3804547114523018 2226 68-69 0.37928807365965866 2226 70-71 0.3457941209061133 2226 72-73 0.24997059727836302 2226 74-75 0.18697243690666454 2226 76-77 0.5626016343317133 2226 78-79 0.30208118123390904 2226 80-81 0.2637893299865084 2226 82-83 0.29807323408829944 2226 84-85 0.19205320570223705 2226 86-87 0.2941416597915918 2226 88-89 0.26309857453701824 2226 90-91 0.4572514879279126 2226 92-93 0.14086477792304208 2226 94-95 0.4254486118694203 2226 96-97 0.344297262638392 2226 98-99 0.35530136082225994 2226 100 0.426144795997061 2227 1 1.1956048881736265 2227 2 1.7946279475993805 2227 3 0.5821205355914447 2227 4 -0.1499196292680658 2227 5 3.07719401854731 2227 6 0.12752097062291767 2227 7 0.39292155352368496 2227 8 0.231985654678887 2227 9 0.27224298208197695 2227 10-11 0.1999094257282792 2227 12-13 0.1909605977828548 2227 14-15 0.16404312286298506 2227 16-17 0.2296587620496311 2227 18-19 0.09079751444493667 2227 20-21 0.15525223797812515 2227 22-23 0.25808385771310327 2227 24-25 0.32844696461631173 2227 26-27 0.2521459623113387 2227 28-29 0.1574718309024803 2227 30-31 0.04895888623970279 2227 32-33 0.14176660373259864 2227 34-35 0.20788328105248866 2227 36-37 0.2712394154484272 2227 38-39 0.18602819769601808 2227 40-41 0.35257922116352347 2227 42-43 0.28737433963047465 2227 44-45 0.33763231744017475 2227 46-47 0.2286242915264367 2227 48-49 0.1425903177561807 2227 50-51 0.2442458115535402 2227 52-53 0.39285164751268553 2227 54-55 0.34724761282421923 2227 56-57 0.4183406305490749 2227 58-59 0.3735499434743019 2227 60-61 0.4382889319976542 2227 62-63 0.400485149180561 2227 64-65 0.3967188381200941 2227 66-67 0.19406084601536122 2227 68-69 0.28152635849809116 2227 70-71 0.5465003624364826 2227 72-73 0.46524750486905475 2227 74-75 0.3954521691315449 2227 76-77 0.46156388955787975 2227 78-79 0.3266518182495801 2227 80-81 0.23830451245747497 2227 82-83 0.1993465102672829 2227 84-85 0.252693051151482 2227 86-87 0.4670973932479612 2227 88-89 0.3772156460723366 2227 90-91 0.4022854120848507 2227 92-93 0.36932246110561096 2227 94-95 0.4922609651420444 2227 96-97 0.24844160295544526 2227 98-99 0.48035623447386655 2227 100 0.39690865382430474 2228 1 1.3930395226611818 2228 2 1.572930709842538 2228 3 -0.03073101093987951 2228 4 -0.054950813589245584 2228 5 3.110509740970361 2228 6 -0.005943167255264115 2228 7 -0.00822401620077784 2228 8 0.06357344798033182 2228 9 0.09688507031447102 2228 10-11 0.13756385575987196 2228 12-13 0.11824891670796944 2228 14-15 0.02921659575007851 2228 16-17 0.1056536954740892 2228 18-19 -0.0024478849503779543 2228 20-21 0.1492148940394813 2228 22-23 0.13521401385933984 2228 24-25 0.09564194503933265 2228 26-27 0.13574086069014868 2228 28-29 0.21486527252901766 2228 30-31 0.08340180178519319 2228 32-33 0.16730011978844317 2228 34-35 0.08517311442102482 2228 36-37 0.13370518546972932 2228 38-39 0.15669261553278346 2228 40-41 0.12215685813984578 2228 42-43 0.42773763122954733 2228 44-45 0.2768259911729558 2228 46-47 0.10184687872839504 2228 48-49 0.15164059097600813 2228 50-51 0.11563033199028894 2228 52-53 0.12024603846148096 2228 54-55 0.20291627618458818 2228 56-57 0.3554590085944085 2228 58-59 0.32773975791737797 2228 60-61 0.3176144212594352 2228 62-63 0.33042516705708636 2228 64-65 0.2727779066987708 2228 66-67 0.3370704496194534 2228 68-69 0.34430949081023954 2228 70-71 0.3498100413155214 2228 72-73 0.4057095503759456 2228 74-75 0.24982176528742173 2228 76-77 0.3374523037404771 2228 78-79 0.4222725560823477 2228 80-81 0.386302021858711 2228 82-83 0.26091026152533203 2228 84-85 0.37600696234086683 2228 86-87 0.42135368057670775 2228 88-89 0.567039338431762 2228 90-91 0.5316935858917233 2228 92-93 0.4249565057123732 2228 94-95 0.6222971755858495 2228 96-97 0.4224387517361947 2228 98-99 0.7592367213074525 2228 100 0.705938050085841 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 13.0 13 26.0 14 114.0 15 417.0 16 1465.0 17 3854.0 18 6270.0 19 8032.0 20 9532.0 21 11158.0 22 13042.0 23 14918.0 24 16868.0 25 19362.0 26 21569.0 27 24502.0 28 27519.0 29 31286.0 30 36300.0 31 41964.0 32 49136.0 33 58993.0 34 71906.0 35 93274.0 36 131369.0 37 218513.0 38 540345.0 39 1000981.0 40 1025.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.859382326710367 18.45903478204904 19.105118468596856 34.57646442264373 2 20.557851281773303 50.15359108050927 12.700150350934326 16.588407286783095 3 39.1174254295359 16.765420154351315 23.820123704382635 20.297030711730155 4 17.558001966783127 15.71311374861284 22.991189414745495 43.73769486985854 5 17.819478977712915 35.192865785594556 26.398908121559096 20.58874711513343 6 17.644970785568066 27.583012634115985 31.12436337316755 23.647653207148398 7 20.437468644969563 26.974027133130352 35.879161431488825 16.70934279041126 8 20.921421186240018 27.854026057227436 33.9183691268029 17.306183629729645 9 20.78481411943256 28.179975735129005 27.997846564018463 23.037363581419974 10-11 19.550072888346953 30.05730405627624 28.952893791673407 21.4397292637034 12-13 24.227051378031938 26.656432004362294 27.356869252936217 21.759647364669547 14-15 21.690997423131016 27.280496447686463 28.724875731175874 22.303630398006646 16-17 20.965190872919973 29.31364729864823 28.528829103825853 21.19233272460594 18-19 21.434757288121503 29.469204928124388 29.710610644184644 19.385427139569465 20-21 22.82760326732153 28.96675011706557 29.772597323365474 18.43304929224743 22-23 21.857945767157496 30.3037021248675 28.64662824660836 19.19172386136665 24-25 22.093747822213565 28.35234434761771 26.772743629860056 22.78116420030867 26-27 20.28400984125134 26.599070892628557 30.02940801294996 23.087511253170142 28-29 19.905014521689 27.839197536335806 30.566642356872265 21.689145585102924 30-31 20.193824752751677 29.898858979296968 28.23369016977752 21.673626098173838 32-33 20.90463058017657 29.833055731363345 28.65997514827287 20.602338540187215 34-35 21.475164778198945 29.040229395542255 29.78380464537384 19.700801180884955 36-37 21.88231659828842 28.051091532032768 28.86659741220897 21.199994457469842 38-39 21.383946919962824 29.357567473337003 28.920742453780036 20.337743152920137 40-41 20.84700138361627 29.684865601750815 29.82387930138586 19.644253713247057 42-43 20.488374936321957 29.810433010697913 29.38098828323994 20.320203769740193 44-45 20.535461393220917 30.130783334752927 28.70007698411372 20.633678287912435 46-47 20.005314432862594 27.846629706090265 29.03858689882261 23.10946896222453 48-49 20.939678932639104 28.260627699691042 30.19574504850325 20.603948319166598 50-51 21.889224384035394 30.61070124010037 28.231570170265712 19.26850420559853 52-53 20.32743331454554 30.117360531209535 28.482476206545083 21.072729947699838 54-55 22.377472015600297 28.20944475910172 28.145245163987166 21.26783806131082 56-57 21.407826871466632 28.85085634364364 29.087784776217163 20.653532008672567 58-59 21.118700639730896 27.869062935164656 29.04611805359262 21.96611837151183 60-61 19.617548572093444 30.37738774883092 29.47442271511416 20.53064096396148 62-63 20.35353359349669 30.951267364008807 27.48772803778829 21.207471004706214 64-65 19.969638339645094 30.177595336122938 28.7639849577331 21.088781366498864 66-67 20.404111579282837 30.169112376021545 29.284467507528262 20.142308537167352 68-69 20.900188405271436 29.99387061370888 29.12098324484983 19.984957736169857 70-71 19.78899261661626 30.60501607129976 29.310855656620692 20.295135655463284 72-73 20.129735959568873 30.66743066641182 28.478762939872105 20.7240704341472 74-75 20.178396114034296 29.09940405574644 30.310304256377883 20.411895573841377 76-77 19.642910268474456 30.392097330089868 28.606852441953208 21.358139959482475 78-79 19.670887819597162 29.42539448754622 28.615084729392077 22.28863296346454 80-81 21.068833418237393 29.156332144676515 28.23922567498726 21.53560876209883 82-83 20.453220026628596 27.893944500526334 29.8505391536964 21.802296319148667 84-85 20.812975063097223 29.061788207696537 28.265212513239923 21.860024215966316 86-87 21.194146273076385 28.789878198824415 28.38871720177214 21.62725832632706 88-89 20.532567866447845 29.33253673046961 28.3433173591637 21.791578043918847 90-91 19.956328122675753 29.423153023144543 27.997602040629193 22.62291681355051 92-93 19.690307052095303 29.242674384911606 28.793790573052792 22.27322798994031 94-95 20.074636689185912 29.365282487683153 28.448156762314706 22.111924060816225 96-97 19.434718979457184 29.251864388958467 28.34559957746358 22.967817054120772 98-99 21.33780376427456 30.090579614166547 27.51745998884158 21.054156632717312 100 20.441503280892576 31.41522394470837 26.454577946517027 21.688694827882024 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 3.0 2 10.0 3 14.5 4 20.5 5 28.5 6 33.0 7 39.5 8 46.5 9 57.0 10 63.5 11 77.0 12 81.0 13 88.5 14 124.5 15 165.0 16 223.5 17 287.0 18 386.5 19 648.0 20 1063.5 21 1609.5 22 2484.5 23 3882.0 24 5978.0 25 8805.5 26 12591.0 27 17367.0 28 22545.5 29 28416.5 30 35255.5 31 42726.0 32 51419.5 33 61398.0 34 72031.5 35 83198.0 36 94431.5 37 103390.5 38 109465.0 39 114752.0 40 118600.0 41 119549.5 42 118160.0 43 116464.0 44 115491.0 45 111564.5 46 103790.0 47 95825.5 48 89192.0 49 85702.5 50 82873.5 51 76160.5 52 66676.0 53 57177.0 54 48042.5 55 38886.0 56 30191.5 57 22132.0 58 15410.0 59 11032.0 60 8733.0 61 7376.0 62 6104.5 63 4740.0 64 3623.5 65 3456.0 66 4225.5 67 3965.0 68 2513.0 69 2040.0 70 2591.5 71 2562.5 72 1581.0 73 669.0 74 307.0 75 167.0 76 118.0 77 95.5 78 74.0 79 69.5 80 59.0 81 44.0 82 32.5 83 21.5 84 21.0 85 24.0 86 22.5 87 20.5 88 17.0 89 14.5 90 13.0 91 13.5 92 14.0 93 14.5 94 17.5 95 13.0 96 7.0 97 15.0 98 38.0 99 80.0 100 82.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.349675374823791 2 0.0065206237139598 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.871620330163631E-4 30-31 0.01010696675663769 32-33 0.0 34-35 0.0 36-37 0.0 38-39 9.984705062000942E-4 40-41 0.0015486481320654524 42-43 1.2226169463674624E-4 44-45 0.0 46-47 0.0023433491472043028 48-49 0.0 50-51 0.0 52-53 0.00130412474279196 54-55 0.01901169351601404 56-57 0.010983175568201038 58-59 0.012205792514568499 60-61 1.426386437428706E-4 62-63 0.012002023023507256 64-65 2.0376949106124374E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2226169463674624E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2453753.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 75.54649030443517 #Duplication Level Percentage of deduplicated Percentage of total 1 86.67186834164364 65.47755461339263 2 8.403565619532687 12.697197771974217 3 2.291926357721537 5.194409770862684 4 0.9152732654985481 2.765827315115791 5 0.49566877242553065 1.8723018055128315 6 0.3102971366372725 1.406511577467702 7 0.20866446945296893 1.1034707822886178 8 0.13802163415545923 0.8341640037222159 9 0.095740531382494 0.6509575013246148 >10 0.43917066369371405 5.666549188553451 >50 0.02166245801600342 1.1006263169736166 >100 0.0076471113975669705 1.0151678792684582 >500 4.936384425905591E-4 0.21526147354311806 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.075389821224875E-5 0.0 3 0.0 0.0 0.0 4.075389821224875E-5 0.0 4 0.0 0.0 0.0 4.075389821224875E-5 0.0 5 0.0 0.0 0.0 4.075389821224875E-5 0.0 6 0.0 0.0 0.0 4.075389821224875E-5 0.0 7 0.0 0.0 0.0 4.075389821224875E-5 0.0 8 0.0 0.0 0.0 4.075389821224875E-5 0.0 9 0.0 0.0 0.0 4.075389821224875E-5 0.0 10-11 2.0376949106124374E-5 0.0 0.0 4.075389821224875E-5 0.0 12-13 4.075389821224875E-5 0.0 0.0 4.075389821224875E-5 0.0 14-15 4.075389821224875E-5 0.0 0.0 4.075389821224875E-5 0.0 16-17 4.075389821224875E-5 0.0 0.0 4.075389821224875E-5 0.0 18-19 4.075389821224875E-5 0.0 0.0 4.075389821224875E-5 0.0 20-21 4.075389821224875E-5 0.0 0.0 4.075389821224875E-5 0.0 22-23 4.075389821224875E-5 2.0376949106124374E-5 0.0 4.075389821224875E-5 0.0 24-25 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 26-27 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 28-29 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 30-31 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 32-33 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 34-35 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 36-37 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 38-39 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 40-41 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 42-43 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 44-45 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 46-47 4.075389821224875E-5 4.075389821224875E-5 0.0 4.075389821224875E-5 0.0 48-49 4.075389821224875E-5 4.075389821224875E-5 0.0 6.113084731837312E-5 0.0 50-51 4.075389821224875E-5 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 52-53 1.0188474553062186E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 54-55 1.2226169463674624E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 56-57 1.8339254195511937E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 58-59 2.0376949106124374E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 60-61 2.0376949106124374E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 62-63 2.0376949106124374E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 64-65 2.0376949106124374E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 66-67 2.0376949106124374E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 68-69 2.4452338927349247E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 70-71 2.4452338927349247E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 72-73 3.2603118569799E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 74-75 4.4829288033473624E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 76-77 5.50177625865358E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 78-79 6.928162696082287E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 80-81 9.373396588817211E-4 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 82-83 0.0011411091499429651 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 84-85 0.0012837477936858354 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 86-87 0.00130412474279196 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 88 0.00130412474279196 4.075389821224875E-5 0.0 8.15077964244975E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGCAGC 65 0.0 81.42898 1 TCACCGG 9140 0.0 77.852745 8 CACCGGC 9260 0.0 74.30943 9 CTTCACC 10000 0.0 72.98797 6 TGCAGCG 30 7.617044E-5 70.571785 1 CGTAGTG 1915 0.0 69.364716 4 CCTTCAC 11380 0.0 68.674095 5 GTGTGGC 3690 0.0 68.56198 7 CGGGGCA 1065 0.0 66.55003 94 TTCACCG 11015 0.0 66.34757 7 GGCGCGC 445 0.0 65.39621 6 CAGCGTA 990 0.0 62.552258 1 AGGCGCG 470 0.0 61.917694 5 ACCTTCA 4175 0.0 60.260017 4 CGGGCCC 595 0.0 59.953915 7 GCGCGCA 495 0.0 58.790535 7 GCCTTCA 3440 0.0 58.53556 4 TGTGGCA 4265 0.0 57.117508 8 GCGTAGT 1400 0.0 56.995667 3 TGTGTGG 4135 0.0 56.642963 6 >>END_MODULE