FastQCFastQC Report
Mon 6 Mar 2017
1HL_33A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_33A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2708943
Sequences flagged as poor quality0
Sequence length92
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA134810.4976479756126282No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG85170.31440307160394293No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT60920.2248847613257274No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA59150.2183508475445958No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA55900.20635354822895868No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG50300.18568127863893777No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG48970.1807716146113078No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG48440.17881513195368082No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT46150.17036165028204728No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC41370.15271639159627942No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA37710.13920558682851578No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC34550.12754052041700398No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT33660.12425510614287566No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA33310.12296308929349935No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32000.11812725480011944No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA31420.1159861983068673No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA30750.11351290890948978No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC29480.10882473348461005No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT27380.10107263238835222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTA500.085.999493
AGCAGCG550.085.999492
GCTGCTC159.829928E-485.999483
AGCTGCT1050.085.999482
GCAGCCT202.8586232E-585.999483
GCAGCAC159.829928E-485.999483
TGCAGCA402.1827873E-1185.999482
CAGCTGC3900.084.896931
CAGCAGC3850.083.7657241
CTGCTGC1250.082.55951
AGCAGCC1050.081.904272
AGCAGCT950.081.47322
CTGCAGC850.080.940691
CAGCCCT157200.080.7476041
GCTGCCC750.080.266183
AGCTGCC1500.080.266182
CAGCGTA58300.079.140181
GCAGCTA600.078.8328553
AGCCCTT132900.078.557842
GCTGCAT501.8189894E-1277.3995363
Produced by FastQC (version 0.11.5)