##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_33A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2708943 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.809434159375078 12.0 12.0 12.0 2.0 27.0 2 14.645426647958264 12.0 12.0 12.0 12.0 22.0 3 13.72613266502839 12.0 12.0 12.0 12.0 22.0 4 13.700668858665539 12.0 12.0 12.0 12.0 22.0 5 22.443135938999085 22.0 22.0 22.0 22.0 27.0 6 26.783796484459067 27.0 22.0 32.0 22.0 32.0 7 34.9691580812147 37.0 37.0 37.0 27.0 41.0 8 37.60392743590397 41.0 37.0 41.0 32.0 41.0 9 38.57094187659172 41.0 41.0 41.0 32.0 41.0 10-11 38.93104210756742 41.0 41.0 41.0 37.0 41.0 12-13 39.10633612445888 41.0 41.0 41.0 37.0 41.0 14-15 39.0600761994623 41.0 41.0 41.0 37.0 41.0 16-17 38.965140647108484 41.0 41.0 41.0 37.0 41.0 18-19 38.8909080036014 41.0 41.0 41.0 37.0 41.0 20-21 38.90461464120877 41.0 41.0 41.0 37.0 41.0 22-23 38.89351861593249 41.0 41.0 41.0 37.0 41.0 24-25 38.923157851604856 41.0 41.0 41.0 37.0 41.0 26-27 38.889178362187764 41.0 41.0 41.0 37.0 41.0 28-29 38.83453527815092 41.0 41.0 41.0 37.0 41.0 30-31 38.699753187867 41.0 41.0 41.0 32.0 41.0 32-33 38.74400291921978 41.0 41.0 41.0 32.0 41.0 34-35 38.7260529291314 41.0 41.0 41.0 32.0 41.0 36-37 38.64880767147925 41.0 41.0 41.0 32.0 41.0 38-39 38.544079185128666 41.0 41.0 41.0 32.0 41.0 40-41 38.59001832079892 41.0 41.0 41.0 32.0 41.0 42-43 38.328035325955554 41.0 41.0 41.0 32.0 41.0 44-45 38.440965904413645 41.0 41.0 41.0 32.0 41.0 46-47 38.46747292209544 41.0 41.0 41.0 32.0 41.0 48-49 38.39410629902512 41.0 41.0 41.0 32.0 41.0 50-51 38.279663691705586 41.0 41.0 41.0 32.0 41.0 52-53 38.24574012816069 41.0 41.0 41.0 32.0 41.0 54-55 38.11734779949228 41.0 37.0 41.0 32.0 41.0 56-57 37.91732181149622 41.0 37.0 41.0 29.5 41.0 58-59 37.91819466116489 41.0 37.0 41.0 29.5 41.0 60-61 37.908010799784265 41.0 37.0 41.0 29.5 41.0 62-63 37.916772150613724 41.0 37.0 41.0 32.0 41.0 64-65 37.905169470158654 41.0 37.0 41.0 32.0 41.0 66-67 37.75071716163832 41.0 37.0 41.0 27.0 41.0 68-69 37.7853022378101 41.0 37.0 41.0 27.0 41.0 70-71 37.76470748923104 41.0 37.0 41.0 27.0 41.0 72-73 37.60754545222989 41.0 37.0 41.0 27.0 41.0 74-75 37.544711719663354 41.0 37.0 41.0 27.0 41.0 76-77 36.48249907805369 39.0 34.5 41.0 24.5 41.0 78-79 37.0059275887311 41.0 37.0 41.0 27.0 41.0 80-81 37.662626160830996 41.0 37.0 41.0 27.0 41.0 82-83 37.85061535071059 41.0 37.0 41.0 32.0 41.0 84-85 37.76770349173091 41.0 37.0 41.0 27.0 41.0 86-87 37.62128568227534 41.0 37.0 41.0 27.0 41.0 88-89 37.54133826366963 41.0 37.0 41.0 27.0 41.0 90-91 37.376790320062106 41.0 37.0 41.0 27.0 41.0 92-93 37.26148667579938 41.0 37.0 41.0 27.0 41.0 94-95 37.09061449428799 41.0 37.0 41.0 27.0 41.0 96-97 37.027021609535524 41.0 37.0 41.0 27.0 41.0 98-99 36.91886244930218 41.0 37.0 41.0 27.0 41.0 100 36.78261336617271 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.611657057436533 1101 2 -0.6113951515282157 1101 3 0.011174224595368543 1101 4 0.05997106848142053 1101 5 -1.1247782804871527 1101 6 -0.9435570450931401 1101 7 -0.43159578734501736 1101 8 -0.1680642143216673 1101 9 -0.23354458906813846 1101 10-11 -0.1641218699115825 1101 12-13 -0.09447502883362091 1101 14-15 -0.17762358860242955 1101 16-17 -0.17365493404925303 1101 18-19 0.020008726761602702 1101 20-21 0.0034972989104602448 1101 22-23 -0.06278098849732316 1101 24-25 -0.16304686929390044 1101 26-27 -0.19905129003807787 1101 28-29 -0.14144431965922877 1101 30-31 -0.1275154927901596 1101 32-33 -0.2648953658473161 1101 34-35 -0.12058382172181581 1101 36-37 -0.11642957459052639 1101 38-39 -0.029060663762699335 1101 40-41 -0.07336031123949738 1101 42-43 0.008944422424761456 1101 44-45 -0.0912093119065176 1101 46-47 -0.2478029524657046 1101 48-49 0.0024020209181472296 1101 50-51 0.16617988820281226 1101 52-53 -0.019399054521926473 1101 54-55 -0.04884910433609946 1101 56-57 0.05755108532039088 1101 58-59 -0.0728706812792268 1101 60-61 -0.14347331345812364 1101 62-63 -0.28583996427677505 1101 64-65 0.01576534633289839 1101 66-67 -0.12184009233108384 1101 68-69 -0.12596329653688798 1101 70-71 -0.22433843686689414 1101 72-73 -0.17615854017982002 1101 74-75 -0.064556688128377 1101 76-77 -0.2999800198006852 1101 78-79 -0.2816430935730807 1101 80-81 -0.11008763293513368 1101 82-83 -0.09650746518035902 1101 84-85 -0.08885547152128481 1101 86-87 -0.11606044992294073 1101 88-89 -0.3878435771676436 1101 90-91 -0.04099813542702435 1101 92-93 -0.029524614064499133 1101 94-95 -0.08573851324785409 1101 96-97 -0.28187462847049005 1101 98-99 -0.3893494990149833 1101 100 -0.5556502012227469 1102 1 1.713673896285096 1102 2 0.7726745748783248 1102 3 -1.9122419680073222E-4 1102 4 0.080291297762761 1102 5 -0.7393226807012248 1102 6 -0.23861310530627478 1102 7 -0.07086466956543092 1102 8 -0.2508348409719332 1102 9 -0.43404505493935375 1102 10-11 -0.3234267799698216 1102 12-13 -0.2225409997459309 1102 14-15 -0.30803022103621913 1102 16-17 -0.49486891947358913 1102 18-19 -0.21397837424974853 1102 20-21 -0.161094992899379 1102 22-23 -0.13998768486773372 1102 24-25 -0.23174152371520762 1102 26-27 -0.15672327669835084 1102 28-29 -0.16676190259726553 1102 30-31 -0.31873749464320866 1102 32-33 -0.2598281793389887 1102 34-35 -0.22270058329332443 1102 36-37 -0.45439239985898894 1102 38-39 -0.2729515937188651 1102 40-41 -0.19659668387594564 1102 42-43 -0.2515387235078066 1102 44-45 -0.20431948623122764 1102 46-47 -0.562066351546278 1102 48-49 -0.3947567995101977 1102 50-51 -0.3751621165792187 1102 52-53 -0.3549920850120145 1102 54-55 -0.37600044751838624 1102 56-57 -0.1798989598405072 1102 58-59 -0.23356233436631868 1102 60-61 -0.12963962556870712 1102 62-63 -0.5122169367939406 1102 64-65 -0.3971789162678405 1102 66-67 -0.33964258206790987 1102 68-69 -0.5417480082037329 1102 70-71 -0.6248546260003494 1102 72-73 -0.17461386880904683 1102 74-75 -0.5203144023973962 1102 76-77 -0.364791776348234 1102 78-79 -0.3524497786728418 1102 80-81 -0.3916033380216959 1102 82-83 -0.3240368101969082 1102 84-85 -0.4175033074942718 1102 86-87 -0.3522316755529644 1102 88-89 -0.5474766929320722 1102 90-91 -0.4598754075384264 1102 92-93 -0.36736038354686684 1102 94-95 -0.2251888043456134 1102 96-97 -0.2637701790481728 1102 98-99 -0.5078587180819341 1102 100 -0.6881833874809615 1103 1 1.4127934957587005 1103 2 0.6881794333489637 1103 3 0.3834523293256904 1103 4 1.004833251542948 1103 5 2.8513809113134982 1103 6 -0.2546833690055763 1103 7 -0.5267904174364588 1103 8 -0.13743653378838872 1103 9 -0.23966090635740045 1103 10-11 -0.19724494059799724 1103 12-13 -0.34176332070768467 1103 14-15 -0.25712756364782763 1103 16-17 -0.3937628748335982 1103 18-19 -0.36202616418381695 1103 20-21 -0.11579297132882971 1103 22-23 -0.07266755673295222 1103 24-25 -0.2104453488199738 1103 26-27 -0.21434103168510887 1103 28-29 -0.1792993952929649 1103 30-31 0.014310141516183705 1103 32-33 -0.1933513839386265 1103 34-35 -0.23498960847514638 1103 36-37 -0.1910848197383359 1103 38-39 -0.20032219232323456 1103 40-41 -0.2598984367454591 1103 42-43 -0.18349909172533074 1103 44-45 -0.5084599100342544 1103 46-47 -0.4847597717703991 1103 48-49 -0.4479337035240434 1103 50-51 -0.25690695743860203 1103 52-53 -0.23229607489849968 1103 54-55 -0.3781476276953768 1103 56-57 -0.28195193279072583 1103 58-59 -0.382759387719652 1103 60-61 -0.4699502828016975 1103 62-63 -0.43242082583660135 1103 64-65 -0.36540663876767354 1103 66-67 -0.49756675334377576 1103 68-69 -0.4634095193718295 1103 70-71 -0.6021364399588762 1103 72-73 -0.27420644683351014 1103 74-75 -0.3165807530975542 1103 76-77 -0.3979747342285407 1103 78-79 -0.43584017050692125 1103 80-81 -0.21180431343879036 1103 82-83 -0.2856473652911333 1103 84-85 -0.5131592536375109 1103 86-87 -0.3600927992088572 1103 88-89 -0.18032992551870564 1103 90-91 -0.6690251630366788 1103 92-93 -0.37939608517218204 1103 94-95 -0.40117340745316454 1103 96-97 -0.4207377099285523 1103 98-99 -0.32561548538199503 1103 100 -0.42093593811734564 1104 1 1.5194651041197904 1104 2 -0.6455726604533822 1104 3 0.036921356025816365 1104 4 0.08781200225692665 1104 5 -1.1303011259481615 1104 6 -0.4848272771371249 1104 7 -0.3090146505646416 1104 8 -0.1764775241809673 1104 9 -0.0622038039017454 1104 10-11 -0.1505699949887287 1104 12-13 -0.10700689703975286 1104 14-15 -0.10507044964015222 1104 16-17 -0.03440595827977688 1104 18-19 -0.09893441766920574 1104 20-21 -0.08201215793876315 1104 22-23 -0.13764575765668496 1104 24-25 -0.22739151002189573 1104 26-27 -0.17068832797926348 1104 28-29 -0.005982031547894451 1104 30-31 -0.07751766503466939 1104 32-33 -0.04671943748862617 1104 34-35 -0.2296751477081287 1104 36-37 -0.1686279205125345 1104 38-39 -0.250948889025139 1104 40-41 -0.08814674512430543 1104 42-43 -0.08555433387904543 1104 44-45 -0.29051567630708064 1104 46-47 -0.3199636220135318 1104 48-49 -0.3384762416724314 1104 50-51 -0.23403771552875696 1104 52-53 -0.17321448131970385 1104 54-55 -0.23789282023961533 1104 56-57 -0.3193422154085894 1104 58-59 -0.25476228214067476 1104 60-61 -0.33104748871205203 1104 62-63 -0.2769494502271854 1104 64-65 -0.3857280200461375 1104 66-67 -0.610493562784761 1104 68-69 -0.3448342569379079 1104 70-71 -0.3224087251823562 1104 72-73 -0.29936255945280266 1104 74-75 -0.5440436315596813 1104 76-77 -0.506917168836658 1104 78-79 -0.36273117084706286 1104 80-81 -0.3181761577604405 1104 82-83 -0.301126260405276 1104 84-85 -0.30039898571638446 1104 86-87 -0.20582181679571931 1104 88-89 -0.1979857867747512 1104 90-91 -0.3051399643741419 1104 92-93 -0.534208300784087 1104 94-95 -0.3366687855783539 1104 96-97 -0.2979328597945283 1104 98-99 -0.6907960069767682 1104 100 -0.5253099794607223 1105 1 1.9679116748731005 1105 2 0.2606161290733304 1105 3 0.01527149192740751 1105 4 -0.131033366137558 1105 5 -1.066144206022674 1105 6 0.5169207740361372 1105 7 0.14731535980268262 1105 8 -0.18685370979980576 1105 9 0.0013695631410257647 1105 10-11 -0.13203449891140906 1105 12-13 -0.1491533390881017 1105 14-15 -0.07418386645440478 1105 16-17 -0.07823500388138882 1105 18-19 -0.11914652010751325 1105 20-21 -0.06429149776784016 1105 22-23 -0.00583033976189995 1105 24-25 -0.021788985151800944 1105 26-27 -0.023347222284741065 1105 28-29 -0.17677241976961255 1105 30-31 0.022302732094196642 1105 32-33 -0.2015851942508533 1105 34-35 -0.026067341136879918 1105 36-37 0.030170137857354007 1105 38-39 -0.0014344008516005147 1105 40-41 -0.007116855118020737 1105 42-43 0.1516333167026218 1105 44-45 -0.06782011047459946 1105 46-47 -0.18544425264697395 1105 48-49 -0.10052851215727543 1105 50-51 -0.0405792442600017 1105 52-53 -0.14300792758798764 1105 54-55 -0.09907972195450299 1105 56-57 -0.18383461305476345 1105 58-59 -0.24321403655408602 1105 60-61 -0.2692925746648527 1105 62-63 -0.2525085449953366 1105 64-65 -0.2349998044559385 1105 66-67 -0.264335103406097 1105 68-69 -0.28896258850858914 1105 70-71 -0.399042412506482 1105 72-73 -0.17930450663160968 1105 74-75 -0.23112004726210955 1105 76-77 -0.2239826748640965 1105 78-79 -0.34709649059649905 1105 80-81 -0.3568635236058597 1105 82-83 -0.18002652136136277 1105 84-85 -0.33000216975613483 1105 86-87 -0.15889274428496947 1105 88-89 -0.32428509151765184 1105 90-91 -0.2454642508125673 1105 92-93 -0.16286293760455095 1105 94-95 -0.09128610213206656 1105 96-97 -0.15321018039504253 1105 98-99 -0.4243005992615707 1105 100 -0.4881487686745345 1106 1 1.2683111709632602 1106 2 -0.772742963778903 1106 3 -0.05462828669205422 1106 4 -0.14874776426416325 1106 5 -1.1663329142739371 1106 6 -0.7298170700675328 1106 7 -0.29284362698498967 1106 8 -0.19890428099736113 1106 9 -0.15849026243023445 1106 10-11 -0.041721763219555896 1106 12-13 -0.26195226042114683 1106 14-15 -0.17627117849848162 1106 16-17 -0.12564335209781063 1106 18-19 0.002902785468819502 1106 20-21 -0.12299499951477344 1106 22-23 -0.2191519145111016 1106 24-25 -0.309497705178849 1106 26-27 -0.27493019458930235 1106 28-29 -0.328303907428257 1106 30-31 -0.11796326503133514 1106 32-33 -0.20373726620692167 1106 34-35 -0.10519747072893182 1106 36-37 -0.4250484517797446 1106 38-39 -0.14550070083203792 1106 40-41 -0.2173390335479226 1106 42-43 -0.02878210626764144 1106 44-45 0.03203362085533712 1106 46-47 -0.3360210216879551 1106 48-49 -0.28300383684242547 1106 50-51 -0.3113727474961081 1106 52-53 -0.28015105593539147 1106 54-55 -0.3688073348174754 1106 56-57 -0.21580169452190745 1106 58-59 -0.3221013594659965 1106 60-61 -0.28119035993088914 1106 62-63 -0.29387785086056084 1106 64-65 -0.532575555153322 1106 66-67 -0.276543017459403 1106 68-69 -0.37451299445314845 1106 70-71 -0.590544488228467 1106 72-73 -0.29849034419257237 1106 74-75 -0.4242038915728088 1106 76-77 -0.4125801842728549 1106 78-79 -0.30204819923673654 1106 80-81 -0.3645355784485247 1106 82-83 -0.44345156408467545 1106 84-85 -0.5626818166065419 1106 86-87 -0.3547772054484568 1106 88-89 -0.49106052250810706 1106 90-91 -0.5092227228225639 1106 92-93 -0.4041762362915051 1106 94-95 -0.5372290294933109 1106 96-97 -0.4079249633667956 1106 98-99 -0.5837976764675545 1106 100 -0.6950627060874979 1107 1 1.3673166264045253 1107 2 -0.5562834680906832 1107 3 -0.19164395113011956 1107 4 -0.16984046272579434 1107 5 -1.1494618520593392 1107 6 -0.27737946220420895 1107 7 -0.29657635165035856 1107 8 -0.16093260690355038 1107 9 -0.17779143196453617 1107 10-11 -0.07335633811075581 1107 12-13 -0.1832233892460735 1107 14-15 -0.21185506485354466 1107 16-17 -0.1497097752605896 1107 18-19 -0.16845109017904036 1107 20-21 -0.1887341677664196 1107 22-23 0.0495648663381445 1107 24-25 -0.07311549261481076 1107 26-27 -0.03316272933264486 1107 28-29 -0.07018628927061599 1107 30-31 -0.20871038110671947 1107 32-33 -0.07739955457461178 1107 34-35 -0.18026002113395379 1107 36-37 -0.19899118478517153 1107 38-39 -0.08405266927745458 1107 40-41 -0.26870519907734547 1107 42-43 -0.16958571726330263 1107 44-45 -0.041495102786093696 1107 46-47 -0.2249797742030495 1107 48-49 -0.16518835984913238 1107 50-51 -0.2445912445247842 1107 52-53 -0.13050265251899873 1107 54-55 -0.23154463412632964 1107 56-57 -0.02156978459449732 1107 58-59 -0.2028540699835375 1107 60-61 -0.07861805193793003 1107 62-63 -0.13179712436753732 1107 64-65 -0.07296925111843677 1107 66-67 -0.295483782432278 1107 68-69 -0.3632965851904615 1107 70-71 -0.06293061759013341 1107 72-73 -0.06034980464887951 1107 74-75 -0.21645190771343437 1107 76-77 -0.28783031708064755 1107 78-79 -0.6549658074854037 1107 80-81 -0.61774223287577 1107 82-83 -0.4843530950213264 1107 84-85 -0.183640984748088 1107 86-87 -0.2006398298955503 1107 88-89 -0.19877878832736684 1107 90-91 -0.07057114853414959 1107 92-93 0.0066163234444758245 1107 94-95 0.22100677383554768 1107 96-97 -0.09865191743043766 1107 98-99 -0.299921368001165 1107 100 -0.4041379509905738 1108 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24-25 -0.045363290525287425 2223 26-27 0.07600394644109087 2223 28-29 0.05280560179425464 2223 30-31 0.08765234620574347 2223 32-33 0.0712776890431499 2223 34-35 0.021756420770223883 2223 36-37 0.0646564426409526 2223 38-39 0.016752350175245567 2223 40-41 0.029194346297721552 2223 42-43 0.16639276455170204 2223 44-45 0.17971927035605262 2223 46-47 0.31872323331575103 2223 48-49 0.1678189403781758 2223 50-51 0.14178120913843628 2223 52-53 0.12833575891656324 2223 54-55 0.11450444569532436 2223 56-57 0.26543542829237055 2223 58-59 0.23877757999897398 2223 60-61 0.1773284094189549 2223 62-63 0.10872446485259957 2223 64-65 0.23728631147991308 2223 66-67 0.21187313639094185 2223 68-69 0.20915175040142486 2223 70-71 0.30604983681623565 2223 72-73 0.23303627683390005 2223 74-75 0.10774498531856835 2223 76-77 0.2947828746923875 2223 78-79 0.3371281173703409 2223 80-81 0.15753056940609156 2223 82-83 0.0910766676120005 2223 84-85 0.17243702823495255 2223 86-87 0.29839368230064167 2223 88-89 0.3130335368168886 2223 90-91 0.3294619101299574 2223 92-93 0.11758961422957981 2223 94-95 0.2503961994344266 2223 96-97 0.26490049557882855 2223 98-99 0.35155493358128354 2223 100 0.5193121167660308 2224 1 1.1736502293071318 2224 2 -0.9099565315633171 2224 3 -0.0547100466013557 2224 4 -0.3698112290310931 2224 5 -0.9964630760495474 2224 6 0.5586659565752967 2224 7 0.2371585123993043 2224 8 0.26725222654635417 2224 9 0.1558319615483157 2224 10-11 0.20376679311847568 2224 12-13 0.17750605973976263 2224 14-15 0.07745332603647626 2224 16-17 0.09633684565216072 2224 18-19 0.15374998846232302 2224 20-21 0.1284029246011258 2224 22-23 0.018837384479425623 2224 24-25 0.1256893410536577 2224 26-27 0.082994078020036 2224 28-29 0.09310165442583695 2224 30-31 0.11951912252153107 2224 32-33 0.11959183377999949 2224 34-35 0.08302286813864157 2224 36-37 -0.06239947841167748 2224 38-39 0.10371781070156061 2224 40-41 0.14062526735035163 2224 42-43 0.20010986981485246 2224 44-45 0.18280315193500485 2224 46-47 0.23586961489469616 2224 48-49 -0.04394092804287908 2224 50-51 0.0616002880858062 2224 52-53 0.12895253523235795 2224 54-55 0.21503898516900932 2224 56-57 0.30449792829237055 2224 58-59 0.23692725105160406 2224 60-61 0.21968038310316018 2224 62-63 0.28265538590522965 2224 64-65 0.1388076930588653 2224 66-67 0.270672478496202 2224 68-69 0.3754757635593151 2224 70-71 0.46743963944781086 2224 72-73 0.52785535578127 2224 74-75 0.37398676163435596 2224 76-77 0.3926447167976477 2224 78-79 0.24748995947560104 2224 80-81 0.2938381351955712 2224 82-83 0.33552568076989786 2224 84-85 0.2754386729717879 2224 86-87 0.39892822177432663 2224 88-89 0.30501544471162845 2224 90-91 0.5163451338141627 2224 92-93 0.18687415370326477 2224 94-95 0.3143353441712762 2224 96-97 0.5058544429472462 2224 98-99 0.29213881516022866 2224 100 0.40664764308181844 2225 1 1.2970140197194961 2225 2 1.950449584047151 2225 3 0.978151945583587 2225 4 1.1161234058114058 2225 5 3.2831953971762395 2225 6 0.036767284916891185 2225 7 0.48360008910911745 2225 8 0.4469762231954846 2225 9 0.43265921574509036 2225 10-11 0.3349526340558455 2225 12-13 0.3125145835833294 2225 14-15 0.3016039773822783 2225 16-17 0.3467846970569042 2225 18-19 0.30845647013077127 2225 20-21 0.36553674723968754 2225 22-23 0.39554530827715695 2225 24-25 0.47849656438859256 2225 26-27 0.48900096599510334 2225 28-29 0.27975233988065895 2225 30-31 0.26973498144118224 2225 32-33 0.34898384735812016 2225 34-35 0.33170617477598796 2225 36-37 0.3427545135713359 2225 38-39 0.3581216259998641 2225 40-41 0.5585444740018843 2225 42-43 0.6254992924719787 2225 44-45 0.4652444946569645 2225 46-47 0.4955607543269025 2225 48-49 0.5302893337506589 2225 50-51 0.4892929221751743 2225 52-53 0.4693000754264176 2225 54-55 0.5400538396462906 2225 56-57 0.600085995047813 2225 58-59 0.5828441969574314 2225 60-61 0.6125869353090607 2225 62-63 0.38546482632632717 2225 64-65 0.5543659587653806 2225 66-67 0.5071340123923846 2225 68-69 0.5038588664976018 2225 70-71 0.5218558022105881 2225 72-73 0.6529701326264217 2225 74-75 0.46023550642249944 2225 76-77 0.6154989696464384 2225 78-79 0.6614885903396228 2225 80-81 0.5783881826753259 2225 82-83 0.36212892399175445 2225 84-85 0.3137657825463265 2225 86-87 0.45166468595166975 2225 88-89 0.5855289457430786 2225 90-91 0.5827044839145756 2225 92-93 0.4944526487861012 2225 94-95 0.4618028689753473 2225 96-97 0.5088584241518177 2225 98-99 0.5769393933762998 2225 100 0.887796441625774 2226 1 1.1883916929869915 2226 2 1.2122841165445557 2226 3 0.8131129199058211 2226 4 1.0540585241224516 2226 5 3.1061358800183108 2226 6 -0.2638954831811162 2226 7 -0.024253378640281653 2226 8 0.010832995358882158 2226 9 0.19955817403200626 2226 10-11 0.06702867328376527 2226 12-13 0.20495719338482132 2226 14-15 0.10252468858540453 2226 16-17 0.12433679128069741 2226 18-19 0.1413821790886871 2226 20-21 0.10977356129099292 2226 22-23 0.21883568537111842 2226 24-25 0.20322610975744482 2226 26-27 0.2347077986594428 2226 28-29 0.22064352045454427 2226 30-31 0.1659289066668137 2226 32-33 0.07757628354075763 2226 34-35 0.1752779926493062 2226 36-37 0.20724663130994259 2226 38-39 0.18157910909216923 2226 40-41 0.1947228369458216 2226 42-43 0.23033156946687683 2226 44-45 0.1233795574373957 2226 46-47 0.1516288444510252 2226 48-49 0.06381448191798 2226 50-51 0.07217689748119938 2226 52-53 0.20234755752466071 2226 54-55 0.32085027540389177 2226 56-57 0.4109708513023804 2226 58-59 0.2894079491812249 2226 60-61 0.2424484344298321 2226 62-63 0.11984512872820119 2226 64-65 0.3332376762037086 2226 66-67 0.28011374372021436 2226 68-69 0.27816919004909124 2226 70-71 0.35553228842562845 2226 72-73 0.30649212720362584 2226 74-75 0.32609909308281715 2226 76-77 0.4744455677111006 2226 78-79 0.28651609855781146 2226 80-81 0.16551467934519337 2226 82-83 0.167701694797735 2226 84-85 0.13660317384173482 2226 86-87 0.23627122416667135 2226 88-89 0.00880955356330304 2226 90-91 0.15905290077806455 2226 92-93 0.040787200136492174 2226 94-95 0.43308840256622716 2226 96-97 0.4087157413378506 2226 98-99 0.4707171372567913 2226 100 0.6100832400805132 2227 1 1.4628204727633651 2227 2 1.914698012808719 2227 3 0.5753506821435845 2227 4 -0.06898287298311523 2227 5 3.1415216172757034 2227 6 -0.0039145550183725675 2227 7 0.16772149140945913 2227 8 0.4527199853742161 2227 9 0.41775495051754774 2227 10-11 0.388107727918964 2227 12-13 0.24940485801965195 2227 14-15 0.2784567778864755 2227 16-17 0.302039569785606 2227 18-19 0.2987715811304881 2227 20-21 0.21151059431144859 2227 22-23 0.3059276041475343 2227 24-25 0.2544648442849464 2227 26-27 0.26973640641531915 2227 28-29 0.34852299392225206 2227 30-31 0.3411635650565543 2227 32-33 0.30937389769132295 2227 34-35 0.2172528626990342 2227 36-37 0.31567002609697425 2227 38-39 0.13401824749387714 2227 40-41 0.3507211167408997 2227 42-43 0.36714115026042293 2227 44-45 0.2741816964748338 2227 46-47 0.19811551286282736 2227 48-49 0.16887506304807687 2227 50-51 0.24540994785328252 2227 52-53 0.3029075590204897 2227 54-55 0.41818115308236514 2227 56-57 0.5104716179154494 2227 58-59 0.60289472978031 2227 60-61 0.2880198416322557 2227 62-63 0.4614740880042163 2227 64-65 0.40209168315931976 2227 66-67 0.1912932059691954 2227 68-69 0.27702581470786214 2227 70-71 0.4973547707561323 2227 72-73 0.37880150232048493 2227 74-75 0.31266466654678027 2227 76-77 0.5616580878113169 2227 78-79 0.33150693659678154 2227 80-81 0.20722964886765283 2227 82-83 0.14823533228324237 2227 84-85 0.26188177224867815 2227 86-87 0.3092658765730789 2227 88-89 0.2936408986887642 2227 90-91 0.3750059317043579 2227 92-93 0.4147457282394029 2227 94-95 0.4841859681022882 2227 96-97 0.5546143614343393 2227 98-99 0.8399469715935766 2227 100 0.4795365140792427 2228 1 1.506219175278634 2228 2 1.6831433642050957 2228 3 -0.37668625601013694 2228 4 -0.10534959995585247 2228 5 3.117259495999196 2228 6 0.11879858321130499 2228 7 0.41993669388046584 2228 8 0.32489776795960523 2228 9 0.15685631224999952 2228 10-11 0.14792448503354194 2228 12-13 0.18409583658208106 2228 14-15 0.07342325065661726 2228 16-17 0.1549009615104069 2228 18-19 0.0462337123506984 2228 20-21 0.0526207007879691 2228 22-23 0.1999000597657954 2228 24-25 0.10510738023721444 2228 26-27 0.11443539872558262 2228 28-29 0.05092211282991599 2228 30-31 0.07403121862413542 2228 32-33 -0.07176110834461014 2228 34-35 0.14128216368892055 2228 36-37 0.14442844611713213 2228 38-39 0.08504645450891957 2228 40-41 0.043593349113045576 2228 42-43 0.33387804989909853 2228 44-45 0.34637966880010396 2228 46-47 0.21576499042391362 2228 48-49 0.21859624475590067 2228 50-51 0.11476410629302336 2228 52-53 0.18099856704448314 2228 54-55 0.1157101271227603 2228 56-57 0.4387263190426296 2228 58-59 0.21882272127575675 2228 60-61 0.13790627014621037 2228 62-63 0.13196510348598878 2228 64-65 0.18873114798676482 2228 66-67 0.3390706800983594 2228 68-69 0.33306704007389953 2228 70-71 0.45424950813750087 2228 72-73 0.2777322859461009 2228 74-75 0.4057377717136603 2228 76-77 0.35270368622126114 2228 78-79 0.1957669649934317 2228 80-81 0.23290261640164545 2228 82-83 0.23717869502383593 2228 84-85 0.16162325287551482 2228 86-87 0.2251739302804765 2228 88-89 0.3762999949308323 2228 90-91 0.30345220020538477 2228 92-93 0.3347922587031533 2228 94-95 0.5034261015427006 2228 96-97 0.18588663922499649 2228 98-99 0.3193876183774407 2228 100 0.5167830568382357 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 23.0 13 38.0 14 118.0 15 422.0 16 1681.0 17 4154.0 18 6886.0 19 9014.0 20 10630.0 21 12232.0 22 13869.0 23 16140.0 24 18261.0 25 21362.0 26 23964.0 27 26986.0 28 30871.0 29 35199.0 30 39998.0 31 46177.0 32 54687.0 33 65611.0 34 80538.0 35 103941.0 36 147247.0 37 243472.0 38 597677.0 39 1096462.0 40 1283.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.45681752709676 18.171815703716533 19.07314002727577 35.29822674191094 2 18.885411313711245 52.36630163202381 11.781670299292966 16.966616754971973 3 40.89698454341786 14.930214478488473 23.936753191189332 20.236047786904336 4 17.78981691382949 16.474137698726036 21.69776182075444 44.03828356669003 5 17.287111615120732 35.48247416058588 26.412146730292957 20.818267494000427 6 17.525802499351222 28.057659389658625 30.709173282715806 23.70736482827435 7 20.192783679833795 26.593508981178264 36.34731332479126 16.866394014196683 8 20.869763594139854 27.625018318953188 34.35731205861474 17.147906028292216 9 20.640338316457747 27.9632683301199 28.240793549365932 23.155599804056415 10-11 19.506944221417726 30.178800366046833 28.77172018754178 21.54253522499366 12-13 24.2903412880965 26.554803847847662 27.446904567574883 21.707950296480952 14-15 21.72406359233103 27.339814828145148 28.67814125287981 22.25798032664401 16-17 20.933626141266167 29.44949376934103 28.269568610339903 21.347311479052898 18-19 21.467136813140772 29.40222441003742 29.86367745648395 19.26696132033786 20-21 22.856313329590176 28.79259548835099 29.93075158835014 18.42033959370869 22-23 21.92787002162836 30.371753853809402 28.620443471863382 19.079932652698858 24-25 22.164715167502603 28.368204868098 26.741186507061983 22.725893457337417 26-27 20.3861616874183 26.35498421339984 29.994890996229895 23.263963102951962 28-29 19.739004298185982 27.858528088637097 30.741636884035728 21.660830729141193 30-31 20.245898870462288 29.878986936230252 28.219060651252114 21.656053542055346 32-33 20.722676704530144 29.911518994677998 28.789974539885115 20.575829760906743 34-35 21.523081142718762 29.1371025525454 29.78962643363112 19.55018987110471 36-37 21.83633985654183 28.036673344548042 28.822201131585274 21.304785667324857 38-39 21.572513662251136 29.232418480547995 28.940291421981566 20.254776435219295 40-41 21.025350247156744 29.723816806658476 29.82503838723235 19.425794558952433 42-43 20.46356105077185 29.85289090722503 29.377871508507745 20.305676533495365 44-45 20.492789992258974 30.02551179556011 28.916702935425363 20.564995276755546 46-47 19.832252355715376 28.024296293999335 29.058926523608097 23.084524826677193 48-49 20.815332770013985 28.099483820811287 30.338290617410557 20.746892791764168 50-51 21.743812992742924 30.57567102740811 28.282304204998038 19.398211774850928 52-53 20.377444440588842 30.346428183817647 28.38203449220385 20.894092883389654 54-55 22.58736262620482 28.120861248082353 28.21348067767072 21.078295448042105 56-57 21.710016615383136 28.642365738160684 29.205804622218317 20.44181302423786 58-59 21.231373631992696 27.814016354616584 29.067637699720848 21.886972313669865 60-61 19.57155110247221 30.470393433285302 29.471310583552317 20.486744880690175 62-63 20.403865951739657 31.046918146370768 27.53511334086361 21.014102561025968 64-65 19.85479193802891 30.122053252923052 28.768756641203453 21.254398167844588 66-67 20.27730003916657 30.29216561588782 29.22846660117987 20.202067743765742 68-69 20.864909302262912 30.013182263340347 29.186863658629953 19.935044775766784 70-71 19.829025564583677 30.718457346647753 29.17185780579363 20.28065928297495 72-73 20.103376113856957 30.74953958056703 28.47834007581555 20.668744229760463 74-75 20.15625651776357 29.13952046979209 30.29144577792888 20.412777234515456 76-77 19.570935970228977 30.492132909404145 28.583067270149282 21.353863850217593 78-79 19.5653618403931 29.527347013207734 28.523431463858785 22.383859682540386 80-81 21.032688824919582 29.346186586773122 28.339076051205307 21.282048537101993 82-83 20.495761631012538 27.89665563284277 29.894353627964854 21.71322910817983 84-85 20.838625988069886 29.085957142693665 28.216374430912722 21.85904243832373 86-87 21.200778310950064 28.555196620969873 28.435186713046377 21.80883835503368 88-89 20.41172516365239 27.83611541475771 28.77858633422704 22.973573087362855 90-91 20.080895020677808 28.61856081874 27.89532670122627 23.405217459355917 92-93 19.860181628037207 28.08377658739959 28.81267712166701 23.243364662896195 94-95 20.100958196610264 28.663209229577735 28.4549730282254 22.780859545586598 96-97 19.608921265600642 29.059304680829385 28.341773894836475 22.990000158733498 98-99 21.210043917498446 29.32621690452697 27.56130343089537 21.90243574707921 100 20.431400734530037 30.73124831345658 26.549395834463844 22.287955117549537 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 6.5 2 11.5 3 17.0 4 23.0 5 19.0 6 20.5 7 38.5 8 49.0 9 49.5 10 57.0 11 73.5 12 92.0 13 97.5 14 120.0 15 166.5 16 218.5 17 298.0 18 429.0 19 676.0 20 1110.5 21 1765.5 22 2770.0 23 4229.5 24 6300.5 25 9512.0 26 13982.0 27 19091.0 28 24572.5 29 30884.5 30 38164.0 31 46418.0 32 55618.0 33 65959.5 34 77241.0 35 89631.0 36 101906.0 37 111401.0 38 118680.5 39 125387.5 40 130189.5 41 131342.0 42 130293.5 43 128558.5 44 127780.0 45 125111.5 46 115890.0 47 105662.0 48 100006.0 49 97565.0 50 94738.0 51 88360.5 52 77181.0 53 64476.5 54 53935.5 55 44474.5 56 34890.5 57 25591.5 58 18020.5 59 12809.5 60 9741.5 61 8038.5 62 6622.0 63 5127.0 64 3798.5 65 3079.5 66 3147.5 67 3094.5 68 2402.5 69 2014.0 70 2192.5 71 2084.5 72 1385.5 73 667.0 74 324.0 75 217.5 76 151.5 77 105.5 78 81.5 79 69.0 80 55.5 81 40.0 82 36.0 83 34.0 84 27.5 85 25.0 86 22.0 87 20.5 88 17.0 89 11.5 90 9.0 91 7.5 92 10.0 93 9.5 94 7.5 95 8.0 96 10.5 97 18.0 98 43.5 99 103.0 100 95.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.325856616399827 2 0.005869447972880935 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.506902876878546E-4 30-31 0.009911614973072523 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.013805753757092E-4 40-41 0.0012735594658137878 42-43 3.6914767125037334E-5 44-45 0.0 46-47 0.00193802527406446 48-49 0.0 50-51 0.0 52-53 0.0015135054521265305 54-55 0.020229292384520457 56-57 0.011388205658074017 58-59 0.012920168493763066 60-61 3.137755205628173E-4 62-63 0.0118680976306995 64-65 9.228691781259332E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.8457383562518664E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2708943.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.73363353605768 #Duplication Level Percentage of deduplicated Percentage of total 1 85.49730971497294 63.04027302842638 2 8.966953785313565 13.223321686821512 3 2.5260193478973156 5.587577547085562 4 1.108756054735325 3.2701045048295825 5 0.5594429323219122 2.0624880078080694 6 0.3363208883754948 1.4878896680400053 7 0.2233159402518877 1.1526126990904986 8 0.1489706623391203 0.8787318579629187 9 0.11673265862402614 0.7746410765406299 >10 0.4846899908258955 6.015708157218385 >50 0.021503702079222354 1.0707009351122363 >100 0.009525962316333483 1.204256743669495 >500 4.583599469110785E-4 0.23169408739481778 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.691476712503733E-5 0.0 3 0.0 0.0 0.0 3.691476712503733E-5 0.0 4 0.0 0.0 0.0 3.691476712503733E-5 0.0 5 0.0 0.0 0.0 3.691476712503733E-5 0.0 6 0.0 0.0 0.0 3.691476712503733E-5 0.0 7 0.0 0.0 0.0 3.691476712503733E-5 0.0 8 0.0 0.0 0.0 3.691476712503733E-5 0.0 9 0.0 0.0 0.0 3.691476712503733E-5 0.0 10-11 5.537215068755599E-5 0.0 0.0 3.691476712503733E-5 0.0 12-13 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 14-15 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 16-17 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 18-19 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 20-21 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 22-23 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 24-25 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 26-27 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 28-29 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 30-31 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 32-33 1.476590685001493E-4 0.0 0.0 3.691476712503733E-5 0.0 34-35 1.8457383562518666E-4 0.0 0.0 3.691476712503733E-5 0.0 36-37 1.8457383562518666E-4 0.0 0.0 3.691476712503733E-5 0.0 38-39 1.8457383562518666E-4 0.0 0.0 3.691476712503733E-5 0.0 40-41 1.8457383562518666E-4 0.0 0.0 3.691476712503733E-5 0.0 42-43 1.8457383562518666E-4 0.0 0.0 3.691476712503733E-5 0.0 44-45 1.8457383562518666E-4 0.0 0.0 3.691476712503733E-5 0.0 46-47 1.8457383562518666E-4 0.0 0.0 3.691476712503733E-5 0.0 48-49 2.0303121918770531E-4 0.0 0.0 3.691476712503733E-5 0.0 50-51 2.21488602750224E-4 0.0 0.0 7.382953425007465E-5 0.0 52-53 2.21488602750224E-4 0.0 0.0 7.382953425007465E-5 0.0 54-55 2.21488602750224E-4 0.0 0.0 7.382953425007465E-5 0.0 56-57 2.3994598631274267E-4 0.0 0.0 7.382953425007465E-5 0.0 58-59 2.7686075343777997E-4 0.0 0.0 7.382953425007465E-5 0.0 60-61 3.3223290412533597E-4 0.0 0.0 7.382953425007465E-5 0.0 62-63 3.87605054812892E-4 0.0 0.0 7.382953425007465E-5 0.0 64-65 4.798919726254853E-4 0.0 0.0 7.382953425007465E-5 0.0 66-67 5.721788904380785E-4 0.0 0.0 7.382953425007465E-5 0.0 68-69 6.090936575631159E-4 0.0 0.0 7.382953425007465E-5 0.0 70-71 6.644658082506719E-4 0.0 0.0 7.382953425007465E-5 0.0 72-73 6.829231918131905E-4 0.0 0.0 7.382953425007465E-5 0.0 74-75 8.121248767508213E-4 0.0 0.0 7.382953425007465E-5 0.0 76-77 8.674970274383773E-4 0.0 0.0 7.382953425007465E-5 0.0 78-79 9.966987123760079E-4 0.0 0.0 7.382953425007465E-5 0.0 80-81 0.0010889856301886012 0.0 0.0 7.382953425007465E-5 0.0 82-83 0.001365846383626381 0.0 0.0 7.382953425007465E-5 0.0 84-85 0.0014027611507514185 0.0 0.0 7.382953425007465E-5 0.0 86-87 0.001439675917876456 3.691476712503733E-5 0.0 7.382953425007465E-5 0.0 88 0.001439675917876456 3.691476712503733E-5 0.0 7.382953425007465E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 10535 0.0 78.906845 8 CACCGGC 10330 0.0 76.38323 9 CTTCACC 12015 0.0 73.75796 6 CAGCTGC 60 1.2551027E-10 70.56656 1 CCTTCAC 13290 0.0 70.14309 5 CGTAGTG 2375 0.0 69.76567 4 GAGCTGC 55 5.0367817E-9 67.35899 1 GTGTGGC 4345 0.0 67.19412 7 TTCACCG 12975 0.0 66.528076 7 AGGCTGC 80 0.0 64.54304 2 CGGGGCA 1220 0.0 63.48261 94 ACCTTCA 5205 0.0 63.396492 4 GCAGCCT 85 0.0 62.264614 1 GCGTAGT 1700 0.0 61.57247 3 CAGCGTA 1255 0.0 61.14832 1 TGTGGCA 5015 0.0 59.808174 8 GCCTTCA 3920 0.0 59.75099 4 CGGGCCC 730 0.0 59.155567 7 GGCCCGA 745 0.0 58.594563 9 AGCGTAG 1550 0.0 57.842674 2 >>END_MODULE