FastQCFastQC Report
Tue 7 Mar 2017
1HL_33A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_33A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2708943
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG105350.078.9068458
CACCGGC103300.076.383239
CTTCACC120150.073.757966
CAGCTGC601.2551027E-1070.566561
CCTTCAC132900.070.143095
CGTAGTG23750.069.765674
GAGCTGC555.0367817E-967.358991
GTGTGGC43450.067.194127
TTCACCG129750.066.5280767
AGGCTGC800.064.543042
CGGGGCA12200.063.4826194
ACCTTCA52050.063.3964924
GCAGCCT850.062.2646141
GCGTAGT17000.061.572473
CAGCGTA12550.061.148321
TGTGGCA50150.059.8081748
GCCTTCA39200.059.750994
CGGGCCC7300.059.1555677
GGCCCGA7450.058.5945639
AGCGTAG15500.057.8426742
Produced by FastQC (version 0.11.5)