FastQCFastQC Report
Tue 7 Mar 2017
1HL_34A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_34A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2899019
Sequences flagged as poor quality0
Sequence length92
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA145760.5027907716368881No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG76550.2640548406202236No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG64260.22166118952652603No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA58680.20241329911946074No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG53030.18292394772162585No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG52850.18230304803107533No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT49700.1714373034464417No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC49030.16912617682050377No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT42260.14577344957035465No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT42110.1452560331615626No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA41250.14228951241782134No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA40270.13890905854704644No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA38820.13390736659538968No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC38600.13314848919582797No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA36110.12455937680987948No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA33240.1146594761883244No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA31730.10945081767315082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCCT402.1827873E-1185.9998863
GCAGCCG202.8585917E-585.9998863
TGCAGCC258.359475E-785.9998862
GCTGCTT600.085.999883
GCTGCTC159.829818E-485.999883
GCTGCCA357.2759576E-1085.999883
GCTGCAC650.085.999883
GCTGCAA850.085.999883
TGCTGCA650.085.999882
GCAGCTC159.829818E-485.999883
GCAGCCA550.085.999883
CAGCTGC5650.085.238821
AGCTGCA2000.083.8498842
CAGCAGC5950.083.831811
AGCAGCC1300.082.6921842
AGCTGCT1800.081.222112
GCTGCAT800.080.624893
AGCAGCA3050.080.360542
CAGCCCT160850.080.279021
AGCTGCC1500.080.266562
Produced by FastQC (version 0.11.5)