##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1HL_34A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2899019
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.845867515873474	12.0	12.0	12.0	2.0	32.0
2	14.66884246015635	12.0	12.0	12.0	12.0	22.0
3	13.741444260972418	12.0	12.0	12.0	12.0	22.0
4	13.73226011971636	12.0	12.0	12.0	12.0	22.0
5	22.44871558275403	22.0	22.0	22.0	22.0	27.0
6	26.82319639850584	27.0	22.0	32.0	22.0	32.0
7	34.99958296237451	37.0	37.0	37.0	27.0	41.0
8	37.62162062408008	41.0	37.0	41.0	32.0	41.0
9	38.568185306822755	41.0	41.0	41.0	32.0	41.0
10-11	38.92218160695049	41.0	41.0	41.0	37.0	41.0
12-13	39.1003770585843	41.0	41.0	41.0	37.0	41.0
14-15	39.05909895726796	41.0	41.0	41.0	37.0	41.0
16-17	38.965667179138876	41.0	41.0	41.0	37.0	41.0
18-19	38.9067636328013	41.0	41.0	41.0	37.0	41.0
20-21	38.92401636553606	41.0	41.0	41.0	37.0	41.0
22-23	38.90909200664087	41.0	41.0	41.0	37.0	41.0
24-25	38.93482536678787	41.0	41.0	41.0	37.0	41.0
26-27	38.90316914100943	41.0	41.0	41.0	37.0	41.0
28-29	38.85549353074264	41.0	41.0	41.0	37.0	41.0
30-31	38.72506475466356	41.0	41.0	41.0	32.0	41.0
32-33	38.76952134497911	41.0	41.0	41.0	32.0	41.0
34-35	38.746188969441036	41.0	41.0	41.0	32.0	41.0
36-37	38.6693321085512	41.0	41.0	41.0	32.0	41.0
38-39	38.56886381220682	41.0	41.0	41.0	32.0	41.0
40-41	38.621567157717834	41.0	41.0	41.0	32.0	41.0
42-43	38.36116458705514	41.0	41.0	41.0	32.0	41.0
44-45	38.47057521872054	41.0	41.0	41.0	32.0	41.0
46-47	38.488603386179946	41.0	41.0	41.0	32.0	41.0
48-49	38.41968041603039	41.0	41.0	41.0	32.0	41.0
50-51	38.317961869170226	41.0	41.0	41.0	32.0	41.0
52-53	38.290414102149725	41.0	41.0	41.0	32.0	41.0
54-55	38.14245853511136	41.0	39.0	41.0	32.0	41.0
56-57	37.94347691408714	41.0	37.0	41.0	29.5	41.0
58-59	37.951720564784154	41.0	37.0	41.0	29.5	41.0
60-61	37.941819456857644	41.0	37.0	41.0	32.0	41.0
62-63	37.94655019508323	41.0	37.0	41.0	32.0	41.0
64-65	37.93504544123374	41.0	37.0	41.0	32.0	41.0
66-67	37.7864322379398	41.0	37.0	41.0	27.0	41.0
68-69	37.82444095744112	41.0	37.0	41.0	27.0	41.0
70-71	37.80719926292308	41.0	37.0	41.0	27.0	41.0
72-73	37.65642308656825	41.0	37.0	41.0	27.0	41.0
74-75	37.593212910988164	41.0	37.0	41.0	27.0	41.0
76-77	36.52445965342069	39.0	34.5	41.0	24.5	41.0
78-79	37.0562569614066	41.0	37.0	41.0	27.0	41.0
80-81	37.700209795106545	41.0	37.0	41.0	27.0	41.0
82-83	37.887567311562975	41.0	37.0	41.0	32.0	41.0
84-85	37.801396955314885	41.0	37.0	41.0	27.0	41.0
86-87	37.66598442438632	41.0	37.0	41.0	27.0	41.0
88-89	37.58958357982476	41.0	37.0	41.0	27.0	41.0
90-91	37.41947689890959	41.0	37.0	41.0	27.0	41.0
92-93	37.30428413887594	41.0	37.0	41.0	27.0	41.0
94-95	37.139936302590634	41.0	37.0	41.0	27.0	41.0
96-97	37.09327465601295	41.0	37.0	41.0	27.0	41.0
98-99	36.96331948842005	41.0	37.0	41.0	27.0	41.0
100	36.84437356222915	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.5510882206384355
1101	2	-0.43811413997307547
1101	3	-0.06789633685200869
1101	4	-0.02086476868553433
1101	5	-1.05504048437081
1101	6	-0.7433522571835809
1101	7	-0.07903361096685302
1101	8	-0.03795654693503536
1101	9	-0.11933714539107854
1101	10-11	0.04119558413702151
1101	12-13	-0.017992716735960812
1101	14-15	0.1391284024338617
1101	16-17	0.08997750933591675
1101	18-19	0.18440234235560382
1101	20-21	0.17572568846495784
1101	22-23	0.15079733661541184
1101	24-25	0.25994238511093215
1101	26-27	0.09497991294101382
1101	28-29	0.09947751695434448
1101	30-31	-6.26650245173721E-4
1101	32-33	-0.008746168576145408
1101	34-35	0.14288663153068626
1101	36-37	0.2882527277294571
1101	38-39	0.1956336952092812
1101	40-41	0.26107132265020283
1101	42-43	0.25281768580773445
1101	44-45	-0.0481789974691722
1101	46-47	0.04166656742053476
1101	48-49	0.09503707490387825
1101	50-51	0.05028172970462208
1101	52-53	0.09776848823346285
1101	54-55	0.22855693845409064
1101	56-57	0.2884849886749876
1101	58-59	0.1611280772076924
1101	60-61	0.15555180029424065
1101	62-63	0.08302285269950715
1101	64-65	0.19051739210270568
1101	66-67	0.13404930643728363
1101	68-69	0.26294640678199954
1101	70-71	0.15226345386172824
1101	72-73	0.14256122957888806
1101	74-75	0.2663874338811141
1101	76-77	-0.29350357743150113
1101	78-79	-0.03646453649299275
1101	80-81	0.11654323659865184
1101	82-83	0.08905383015132884
1101	84-85	0.017230336399123303
1101	86-87	0.02147103826599306
1101	88-89	-0.12288195995710538
1101	90-91	-0.12323075574874309
1101	92-93	0.11004956632498875
1101	94-95	0.13656807751802802
1101	96-97	-0.06514539099466532
1101	98-99	-0.07003765739841583
1101	100	-0.1570692509722349
1102	1	1.566311746098739
1102	2	0.7875557474352739
1102	3	-0.12821219386313487
1102	4	0.11192546562050154
1102	5	-0.8247990794573283
1102	6	-0.197620165395211
1102	7	-0.14885113781371473
1102	8	-0.29047391119667765
1102	9	-0.32244041315103544
1102	10-11	-0.2689394175761777
1102	12-13	-0.27203566310252114
1102	14-15	-0.18180024646019177
1102	16-17	-0.2071234540568767
1102	18-19	-0.17951342705758577
1102	20-21	-0.07164668090553761
1102	22-23	-0.1555064855213999
1102	24-25	-0.2428018172157067
1102	26-27	-0.20247414889058035
1102	28-29	-0.1964720317993809
1102	30-31	-0.18334094378071342
1102	32-33	-0.19733779792859707
1102	34-35	-0.050650067638677854
1102	36-37	-0.20519511243382738
1102	38-39	-0.13539624495467706
1102	40-41	-0.3129323276740692
1102	42-43	-0.3155839101479998
1102	44-45	-0.1759687980915885
1102	46-47	-0.4611300767909654
1102	48-49	-0.2849146847135984
1102	50-51	-0.20095565271637383
1102	52-53	-0.28450609316956843
1102	54-55	-0.2614540358946016
1102	56-57	-0.1347633139611375
1102	58-59	-0.07385001407993741
1102	60-61	-0.05496976423942357
1102	62-63	-0.3352463928570799
1102	64-65	-0.313628592571348
1102	66-67	-0.24502770408616215
1102	68-69	-0.30382518466465314
1102	70-71	-0.3435435021775177
1102	72-73	-0.27364509678864835
1102	74-75	-0.30906075199876426
1102	76-77	-0.19387368488045809
1102	78-79	-0.28720210622839915
1102	80-81	-0.28040276948276244
1102	82-83	-0.3794836533394772
1102	84-85	-0.381675242986816
1102	86-87	-0.17575227927223835
1102	88-89	-0.44690237322991067
1102	90-91	-0.4004753145571627
1102	92-93	-0.22939445127087765
1102	94-95	-0.17346204042545565
1102	96-97	-0.16044798220816858
1102	98-99	-0.19894749980208815
1102	100	-0.08156185651268544
1103	1	1.3673368860775028
1103	2	0.7374133040908042
1103	3	0.5273552781929158
1103	4	1.0424019993588836
1103	5	2.8948355598002706
1103	6	-0.19983043902331943
1103	7	-0.5901259884789596
1103	8	-0.18696780186222384
1103	9	-0.1453497737226428
1103	10-11	-0.2811529061477529
1103	12-13	-0.4234299878482304
1103	14-15	-0.4630671413962162
1103	16-17	-0.40822664432312905
1103	18-19	-0.39156139627450415
1103	20-21	-0.2674274757626378
1103	22-23	-0.15034154071230432
1103	24-25	-0.265465127866527
1103	26-27	-0.18531407421849622
1103	28-29	-0.30759039885927564
1103	30-31	-0.36814226718204424
1103	32-33	-0.4493585971057712
1103	34-35	-0.44825600222336703
1103	36-37	-0.35929090016718135
1103	38-39	-0.25445762588591947
1103	40-41	-0.3823369603322462
1103	42-43	-0.3647899698140833
1103	44-45	-0.7474449343574676
1103	46-47	-0.5639716912200328
1103	48-49	-0.485159039254043
1103	50-51	-0.4064112610659265
1103	52-53	-0.22432307372229587
1103	54-55	-0.30490302346997566
1103	56-57	-0.3219662691974392
1103	58-59	-0.520245227330534
1103	60-61	-0.35506228805166984
1103	62-63	-0.36995255518785797
1103	64-65	-0.5120472736533515
1103	66-67	-0.5494082843206627
1103	68-69	-0.5540462120274654
1103	70-71	-0.8109185761934299
1103	72-73	-0.4818855243258895
1103	74-75	-0.5530220583960599
1103	76-77	-0.643376938440916
1103	78-79	-0.6576111575500718
1103	80-81	-0.26848047818251786
1103	82-83	-0.40962830067839207
1103	84-85	-0.6028049565366089
1103	86-87	-0.3837007246398585
1103	88-89	-0.2835542042720789
1103	90-91	-0.7204298469681234
1103	92-93	-0.4996638220901133
1103	94-95	-0.49997973548681074
1103	96-97	-0.3305952542470152
1103	98-99	-0.446330020933118
1103	100	-0.3731289719109441
1104	1	1.786251553571443
1104	2	-0.7424363107520691
1104	3	-0.09728351972656135
1104	4	0.07526857412489996
1104	5	-1.0309994893732473
1104	6	-0.3493596566752508
1104	7	-0.2598931554287489
1104	8	-0.07737158513346998
1104	9	0.01566160461198507
1104	10-11	-0.0710002193046364
1104	12-13	-0.12988199519360677
1104	14-15	-0.08884974590401384
1104	16-17	-0.003757401853022202
1104	18-19	-0.0937971902435848
1104	20-21	0.15246149916149676
1104	22-23	-0.03814138534850997
1104	24-25	-0.05661949218838913
1104	26-27	-0.11467842735716971
1104	28-29	-0.05855594987539092
1104	30-31	-0.20092931494452415
1104	32-33	-0.0743662589680838
1104	34-35	-0.11112546035144533
1104	36-37	-0.0923572462657063
1104	38-39	-0.08727844838840326
1104	40-41	-0.052915346500114424
1104	42-43	-0.058072330393208915
1104	44-45	-0.07658931386546186
1104	46-47	-0.25490608191120856
1104	48-49	-0.3679092475315997
1104	50-51	-0.027227884242407185
1104	52-53	-0.015144225215770746
1104	54-55	-0.1912277250638894
1104	56-57	-0.14378340650051058
1104	58-59	-0.0899956945477669
1104	60-61	-0.030769587398438603
1104	62-63	-0.1645879068329421
1104	64-65	-0.2002602959421793
1104	66-67	-0.5060781074661165
1104	68-69	-0.2848690397487772
1104	70-71	-0.04750750258200753
1104	72-73	-0.017435314547221026
1104	74-75	-0.12501479307420738
1104	76-77	-0.2809997784211973
1104	78-79	-0.1877000726803999
1104	80-81	-0.17759421649582663
1104	82-83	-0.38868749278660886
1104	84-85	-0.1691457386595161
1104	86-87	-0.2426367702933021
1104	88-89	-0.28265761001679124
1104	90-91	-0.26732200797605543
1104	92-93	-0.2814130158745627
1104	94-95	-0.17646041383370203
1104	96-97	-0.30394229345323254
1104	98-99	-0.3735773546050325
1104	100	-0.3564805269249902
1105	1	1.594022538886632
1105	2	0.36915867745304887
1105	3	-0.06081393549315628
1105	4	-0.018241937019022103
1105	5	-1.1548003025292104
1105	6	0.48279267308688745
1105	7	0.10139302019256746
1105	8	0.04753204352045515
1105	9	0.12476760581935764
1105	10-11	-0.09337109988417325
1105	12-13	-0.1982479190129709
1105	14-15	-0.12908463136151482
1105	16-17	-0.05265449543869494
1105	18-19	-0.08280403806413972
1105	20-21	-0.16009759647524646
1105	22-23	-0.1415585981194667
1105	24-25	-0.09573913691149016
1105	26-27	-0.07488691070250297
1105	28-29	-0.06973881420150718
1105	30-31	-0.08635575512034421
1105	32-33	-0.14183786881807947
1105	34-35	-0.014326049731934631
1105	36-37	-0.2202632363206618
1105	38-39	-0.031179263483913644
1105	40-41	-0.07218264545979025
1105	42-43	0.09704023012678675
1105	44-45	0.047856550894628924
1105	46-47	-0.17224840869128855
1105	48-49	-0.017988678128475044
1105	50-51	-0.034886678764152634
1105	52-53	-0.1750279250026594
1105	54-55	0.019813249123131982
1105	56-57	0.13562152165130925
1105	58-59	-0.13274275067589514
1105	60-61	-0.1305540853097895
1105	62-63	-0.22728428617965335
1105	64-65	-0.22960309791178446
1105	66-67	-0.16570936281502924
1105	68-69	-0.21567562548775498
1105	70-71	-0.368048903469834
1105	72-73	-0.08051733080549894
1105	74-75	-0.2792404644612603
1105	76-77	-0.17114417179084995
1105	78-79	-0.18871469930507345
1105	80-81	-0.24180389872495311
1105	82-83	-0.14183789633404587
1105	84-85	-0.10775749426662884
1105	86-87	-0.19533177377237365
1105	88-89	-0.1388700464056356
1105	90-91	-0.12470120437237853
1105	92-93	0.008692919873020344
1105	94-95	-0.09437005872650417
1105	96-97	-0.15811577884721828
1105	98-99	-0.28215852594722435
1105	100	-0.3222450608149785
1106	1	1.3825963007782587
1106	2	-0.6151354506925877
1106	3	-0.023870342865107474
1106	4	-0.20056670171037538
1106	5	-1.2668795097360253
1106	6	-0.706542225064716
1106	7	-0.2797033261009929
1106	8	-0.018570711677426743
1106	9	-0.19847913970117048
1106	10-11	-0.1288264926481517
1106	12-13	-0.1299673982962517
1106	14-15	-0.09268953638173372
1106	16-17	-0.013079736606393055
1106	18-19	0.0051381177434493
1106	20-21	-0.06249476105337948
1106	22-23	0.005740738274994328
1106	24-25	-0.21714550831785573
1106	26-27	-0.17964004619810225
1106	28-29	-0.11932444383354124
1106	30-31	0.05258225968773189
1106	32-33	-0.17584021604548639
1106	34-35	-0.1117869157542799
1106	36-37	-0.33535084270756954
1106	38-39	-0.10954186504837082
1106	40-41	-0.13612476160608367
1106	42-43	0.09678693869020094
1106	44-45	0.008674799415892664
1106	46-47	-0.2281045788166054
1106	48-49	-0.2948862456292929
1106	50-51	-0.17693449946625606
1106	52-53	-0.1336837316863111
1106	54-55	-0.1208572430554824
1106	56-57	-0.1579419012624541
1106	58-59	-0.15207537731710374
1106	60-61	-0.1208095761453265
1106	62-63	-0.21505779135454617
1106	64-65	-0.5066313673994571
1106	66-67	-0.27693990850033146
1106	68-69	-0.3245697604839748
1106	70-71	-0.5371921474390646
1106	72-73	-0.2552645351350691
1106	74-75	-0.3230495169710039
1106	76-77	-0.1990044270293012
1106	78-79	-0.1581137586113286
1106	80-81	-0.36401746757425
1106	82-83	-0.3006034025473454
1106	84-85	-0.446437890951664
1106	86-87	-0.35582434899811943
1106	88-89	-0.4621749317754862
1106	90-91	-0.5341648394740659
1106	92-93	-0.3754334590329407
1106	94-95	-0.3464946420575856
1106	96-97	-0.4178030495553742
1106	98-99	-0.54937378040146
1106	100	-0.637620842548067
1107	1	1.1725221521890283
1107	2	-0.39650257544718315
1107	3	0.06679627332355054
1107	4	-0.2811963067019292
1107	5	-1.2514747031479239
1107	6	-0.3636883787305898
1107	7	-0.36883126441686187
1107	8	-0.42037205547412526
1107	9	-0.20547214669417713
1107	10-11	-0.08061007900250416
1107	12-13	-0.24155805049015555
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2220	100	0.27792191079629447
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2221	2	1.4887549118184644
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2221	4	-0.20268335899944745
2221	5	-0.7019329620308596
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2221	7	0.17529493234554394
2221	8	0.33538529400271955
2221	9	0.31215237437861987
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2221	12-13	0.26362636426303254
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2221	18-19	0.32975296649622265
2221	20-21	0.22880684921374694
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2221	36-37	0.28643063252217615
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2221	66-67	0.3839514989427997
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2221	76-77	0.7439610366258194
2221	78-79	0.5101229854874845
2221	80-81	0.39273842021611927
2221	82-83	0.5213133779901256
2221	84-85	0.2962757172791797
2221	86-87	0.23501909874187987
2221	88-89	0.39958725240048665
2221	90-91	0.43388837168259187
2221	92-93	0.3818663293013316
2221	94-95	0.5201653263265129
2221	96-97	0.5090758187028115
2221	98-99	0.44585838207208184
2221	100	0.5078524580976449
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2222	2	1.7425524714195078
2222	3	0.6517105693178564
2222	4	0.7751407278168934
2222	5	3.1997586285098656
2222	6	0.2529580491447945
2222	7	0.45792380035167923
2222	8	0.41642480085514677
2222	9	0.3973404720819502
2222	10-11	0.43588894521437993
2222	12-13	0.23739338611127891
2222	14-15	0.26603940729589937
2222	16-17	0.3146030645655813
2222	18-19	0.324650671577551
2222	20-21	0.18307558143828118
2222	22-23	0.3720077855568604
2222	24-25	0.2524174575392948
2222	26-27	0.24704567780803188
2222	28-29	0.23104996953973256
2222	30-31	0.2380079134714279
2222	32-33	0.22131593453141818
2222	34-35	0.2589981651220228
2222	36-37	0.17550191786011737
2222	38-39	0.26182530192883036
2222	40-41	0.31795466966833175
2222	42-43	0.3701093656614276
2222	44-45	0.3737599549799526
2222	46-47	0.27131931541914867
2222	48-49	0.42428755535750895
2222	50-51	0.26209796180108924
2222	52-53	0.29900292791027283
2222	54-55	0.29720660744850846
2222	56-57	0.3679460901329463
2222	58-59	0.4662626383983337
2222	60-61	0.46069111624476733
2222	62-63	0.4691177505747035
2222	64-65	0.4176376361169858
2222	66-67	0.5294741576058684
2222	68-69	0.5429620172530463
2222	70-71	0.4965577717156364
2222	72-73	0.6016842616654969
2222	74-75	0.7241772015784065
2222	76-77	0.6707110595619028
2222	78-79	0.5638506446529661
2222	80-81	0.3542304887010701
2222	82-83	0.25130503362562706
2222	84-85	0.3113274654453875
2222	86-87	0.4839839169789002
2222	88-89	0.5721050224018427
2222	90-91	0.48218695139312473
2222	92-93	0.5278509861557268
2222	94-95	0.7053287404385387
2222	96-97	0.6978349549081742
2222	98-99	0.5744721253714715
2222	100	0.6190946325254458
2223	1	1.286451378829355
2223	2	-0.93977125388888
2223	3	-0.0813095006105442
2223	4	-0.02200972185171146
2223	5	-0.983131438851661
2223	6	0.2194264378871047
2223	7	0.4443132562616867
2223	8	0.4269303586322195
2223	9	0.27474735305058573
2223	10-11	0.1833905356867973
2223	12-13	0.2999142310493852
2223	14-15	0.26439720451052295
2223	16-17	0.2486312000662636
2223	18-19	0.24324111139774374
2223	20-21	0.2558680697520188
2223	22-23	0.22910689483411062
2223	24-25	0.21392140418090833
2223	26-27	0.29039827433792453
2223	28-29	0.33769063569302205
2223	30-31	0.2227851425628984
2223	32-33	0.25509848537559776
2223	34-35	0.30125176412973076
2223	36-37	0.2100941835950323
2223	38-39	0.21991628102539096
2223	40-41	0.30860034908381095
2223	42-43	0.3544723152499998
2223	44-45	0.3365944125575595
2223	46-47	0.14625332463778307
2223	48-49	0.18613628382355785
2223	50-51	0.2232299736308292
2223	52-53	0.23018778475029222
2223	54-55	0.3543663380256845
2223	56-57	0.48788793675726794
2223	58-59	0.5672577822917049
2223	60-61	0.30196437017379907
2223	62-63	0.27889724969084284
2223	64-65	0.3541398802400906
2223	66-67	0.4052768878052859
2223	68-69	0.4542653868427493
2223	70-71	0.3311835639687999
2223	72-73	0.5159535057996649
2223	74-75	0.4998201860671472
2223	76-77	0.5724277948240584
2223	78-79	0.5688485550661468
2223	80-81	0.3517074872395227
2223	82-83	0.25569345696543877
2223	84-85	0.4173779979407968
2223	86-87	0.5142076948470873
2223	88-89	0.4478405249474733
2223	90-91	0.5425379583236776
2223	92-93	0.5017465754977835
2223	94-95	0.5330605696186055
2223	96-97	0.5997425323087384
2223	98-99	0.5925579648993491
2223	100	0.6710617544193695
2224	1	1.1650196845063245
2224	2	-0.9741688450291281
2224	3	0.08558360388738073
2224	4	-0.3077635894137867
2224	5	-1.0956471435688933
2224	6	0.5065506852653421
2224	7	0.4245843705121075
2224	8	0.1161867454131098
2224	9	0.3395521561965822
2224	10-11	0.08190530135301799
2224	12-13	0.09264869116557861
2224	14-15	0.15501553845304272
2224	16-17	0.19979790336257253
2224	18-19	0.11867772898067841
2224	20-21	0.013585123929033216
2224	22-23	0.19756362503264313
2224	24-25	0.12004495109056279
2224	26-27	0.10836620439448552
2224	28-29	0.0021679462697434815
2224	30-31	0.03136843897276975
2224	32-33	-0.008669512612314634
2224	34-35	0.13191612802550878
2224	36-37	0.16489633060176345
2224	38-39	0.12154863139132033
2224	40-41	0.0359569087488083
2224	42-43	0.3215554336045088
2224	44-45	0.21219130934068886
2224	46-47	0.3063738616480265
2224	48-49	0.17869483347749338
2224	50-51	0.13755581962881536
2224	52-53	0.1525366992002759
2224	54-55	0.2490188303892893
2224	56-57	0.3125416102801495
2224	58-59	0.2067700281514817
2224	60-61	0.2952054653493761
2224	62-63	0.36553293198367953
2224	64-65	0.22068518915731516
2224	66-67	0.1234358307088641
2224	68-69	0.2591859142156707
2224	70-71	0.3450968563004366
2224	72-73	0.37816730973192847
2224	74-75	0.41484018350503504
2224	76-77	0.35048047452774966
2224	78-79	0.25003765337738315
2224	80-81	0.21789022139406455
2224	82-83	0.21532660417314275
2224	84-85	0.2578496565822306
2224	86-87	0.3290401171403303
2224	88-89	0.2804103161819782
2224	90-91	0.24237438833147706
2224	92-93	0.10414015657291031
2224	94-95	0.477431346680369
2224	96-97	0.23676464760633564
2224	98-99	0.20857571872225122
2224	100	0.4466071150490407
2225	1	1.4442772772341481
2225	2	1.6313893468133571
2225	3	0.806147508122347
2225	4	1.1543549360123393
2225	5	3.177327260365317
2225	6	-0.21399697799849804
2225	7	0.3133413958394584
2225	8	0.5017429706216348
2225	9	0.22918220754463192
2225	10-11	0.18460146496987306
2225	12-13	0.19973198401952175
2225	14-15	0.09749762244720728
2225	16-17	0.16809545389930491
2225	18-19	0.12412746066507196
2225	20-21	0.11847153014761602
2225	22-23	0.22149993739317608
2225	24-25	0.2215623893165528
2225	26-27	0.026810839071558235
2225	28-29	0.1152757535408
2225	30-31	0.2688724143195671
2225	32-33	0.030881976729034477
2225	34-35	0.19273228997693082
2225	36-37	0.2758109423672579
2225	38-39	0.1468433533414668
2225	40-41	0.2708541344070525
2225	42-43	0.313164240581159
2225	44-45	0.28192413355403545
2225	46-47	0.28291252387037247
2225	48-49	0.15374552745419123
2225	50-51	0.1395459291726837
2225	52-53	0.23473033468851412
2225	54-55	0.2233247281945978
2225	56-57	0.3636168806849369
2225	58-59	0.2968265821658491
2225	60-61	0.3564097865107456
2225	62-63	0.42809204542745505
2225	64-65	0.3426817537489839
2225	66-67	0.4761559866694398
2225	68-69	0.4129095850176867
2225	70-71	0.45878741914457777
2225	72-73	0.5750197652338827
2225	74-75	0.33924865042527586
2225	76-77	0.5002121146266418
2225	78-79	0.46264259588877366
2225	80-81	0.3140352201937091
2225	82-83	0.21615592721698818
2225	84-85	0.290309821171185
2225	86-87	0.2185422339294476
2225	88-89	0.3971992133816471
2225	90-91	0.3961392521420706
2225	92-93	0.3918895625224792
2225	94-95	0.5358432543241705
2225	96-97	0.587560617601298
2225	98-99	0.48934195002262015
2225	100	0.7594243122885445
2226	1	1.5814202865384814
2226	2	1.497657148103123
2226	3	0.7900576075325159
2226	4	0.8095750918684317
2226	5	3.1745514888489375
2226	6	-0.2993765982256811
2226	7	0.24067542250481466
2226	8	0.3057591402574076
2226	9	0.3616610829584914
2226	10-11	0.15268674959604311
2226	12-13	0.3042973390400263
2226	14-15	0.22221570698385307
2226	16-17	0.28178420366968027
2226	18-19	0.12812401813992125
2226	20-21	0.25777486909182556
2226	22-23	0.21484623878796327
2226	24-25	0.22878134001956596
2226	26-27	0.34521793666921496
2226	28-29	0.2403030549852474
2226	30-31	0.23436645527441868
2226	32-33	0.2363597535028461
2226	34-35	0.2300654818912733
2226	36-37	0.21233471003240822
2226	38-39	0.02416435011195972
2226	40-41	0.18267031924187904
2226	42-43	0.3084474485622124
2226	44-45	0.26973467439490406
2226	46-47	0.22135472289775748
2226	48-49	0.24816992923092585
2226	50-51	0.18942529418746545
2226	52-53	0.33339221983754186
2226	54-55	0.36282770427644095
2226	56-57	0.3074770326634777
2226	58-59	0.30849394794895346
2226	60-61	0.33685626760138376
2226	62-63	0.27267015807635886
2226	64-65	0.2926885454507797
2226	66-67	0.30722661617941327
2226	68-69	0.3898483166868232
2226	70-71	0.5341593022171978
2226	72-73	0.19915509482888893
2226	74-75	0.3684493030270417
2226	76-77	0.48087850324243675
2226	78-79	0.42908388476827497
2226	80-81	0.2489915857536218
2226	82-83	0.2569997856934805
2226	84-85	0.26456343707921093
2226	86-87	0.22274396362929139
2226	88-89	0.26770023453047287
2226	90-91	0.4258939370699366
2226	92-93	0.3449624186335427
2226	94-95	0.5320373581695961
2226	96-97	0.4435207398399541
2226	98-99	0.32808742695866044
2226	100	0.47762269221335174
2227	1	1.400673737649198
2227	2	1.6549282056819727
2227	3	0.7241821975308191
2227	4	-0.16712051397184524
2227	5	3.2245500454986313
2227	6	0.06361247200413445
2227	7	0.3326381864725363
2227	8	0.4191484394988052
2227	9	0.1939864766623529
2227	10-11	0.29371320019316727
2227	12-13	0.3640103949854847
2227	14-15	0.1290360182904564
2227	16-17	0.22634782474692372
2227	18-19	0.28204514987089624
2227	20-21	0.3047129216995401
2227	22-23	0.2623470476655285
2227	24-25	0.3412291538851093
2227	26-27	0.4303335069309071
2227	28-29	0.29762390814961037
2227	30-31	0.3030487305718381
2227	32-33	0.44226885972020114
2227	34-35	0.3599972294637439
2227	36-37	0.39361920760818236
2227	38-39	0.27779212089350835
2227	40-41	0.3431305795982169
2227	42-43	0.57944548921418
2227	44-45	0.44924866226353544
2227	46-47	0.3838227436675652
2227	48-49	0.41704612465574087
2227	50-51	0.23386815495588564
2227	52-53	0.401731549088602
2227	54-55	0.4328062885662973
2227	56-57	0.4896431767170455
2227	58-59	0.48616676208511933
2227	60-61	0.32864931762910743
2227	62-63	0.4424759613068119
2227	64-65	0.4256559894818679
2227	66-67	0.4939967470070954
2227	68-69	0.3322748772191275
2227	70-71	0.5346966495077297
2227	72-73	0.6197920272764819
2227	74-75	0.4750132091659651
2227	76-77	0.6200391228727185
2227	78-79	0.43580192869183065
2227	80-81	0.3071133479881212
2227	82-83	0.4788117556381124
2227	84-85	0.4527606805206901
2227	86-87	0.3489451017110028
2227	88-89	0.41700775715198546
2227	90-91	0.37297861001277255
2227	92-93	0.5755916051011738
2227	94-95	0.6438356643980043
2227	96-97	0.49830340135386564
2227	98-99	0.6248407006575647
2227	100	0.6905159600666977
2228	1	1.3244882516499565
2228	2	1.6018285813268527
2228	3	-0.1532504732005311
2228	4	-0.2010714404650198
2228	5	3.3224398025485122
2228	6	0.1681445028891133
2228	7	0.23714648650732073
2228	8	0.5353871972671129
2228	9	0.35687659868857224
2228	10-11	0.3267068395569481
2228	12-13	0.4246862336405286
2228	14-15	0.4097444920362463
2228	16-17	0.38083078330718223
2228	18-19	0.3423088016852418
2228	20-21	0.30194161350909354
2228	22-23	0.3838011252196054
2228	24-25	0.4186539340348929
2228	26-27	0.2919508072230954
2228	28-29	0.22422487612005426
2228	30-31	0.27447662439830367
2228	32-33	0.37313421418610915
2228	34-35	0.3479937930711401
2228	36-37	0.40484186061084415
2228	38-39	0.4471313157172858
2228	40-41	0.4507188070869361
2228	42-43	0.5283295925293814
2228	44-45	0.4905857789386445
2228	46-47	0.4217041845764129
2228	48-49	0.30802916995330065
2228	50-51	0.37886837807906915
2228	52-53	0.34535105342413175
2228	54-55	0.4445572706777199
2228	56-57	0.6255941292166369
2228	58-59	0.5473657581194402
2228	60-61	0.4344996223621891
2228	62-63	0.5182703906744379
2228	64-65	0.46555337054760315
2228	66-67	0.39864645690261824
2228	68-69	0.37216664326427207
2228	70-71	0.555435875834732
2228	72-73	0.7344195696617604
2228	74-75	0.4549398799522848
2228	76-77	0.44766493553613174
2228	78-79	0.5229694345085463
2228	80-81	0.4752356348618818
2228	82-83	0.36281230118169105
2228	84-85	0.3489570507940556
2228	86-87	0.4724444505163774
2228	88-89	0.3711697581798248
2228	90-91	0.488922579199901
2228	92-93	0.37277011635825374
2228	94-95	0.6529082816861873
2228	96-97	0.4673773455600667
2228	98-99	0.7080393473785023
2228	100	0.5829611271741371
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	29.0
13	40.0
14	145.0
15	458.0
16	1796.0
17	4515.0
18	7642.0
19	9581.0
20	11115.0
21	13124.0
22	15038.0
23	17236.0
24	19603.0
25	22097.0
26	25237.0
27	28530.0
28	32189.0
29	36924.0
30	42315.0
31	49227.0
32	57688.0
33	69304.0
34	85022.0
35	109822.0
36	154917.0
37	256644.0
38	630085.0
39	1197199.0
40	1497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.87871150953351	18.32072139043317	21.058127947814047	34.742439152219276
2	18.69446745154911	52.26852735020593	12.341631874290023	16.695373323954943
3	40.73257194933873	14.838157321493926	24.904597037825553	19.524673691341796
4	17.8634220748467	16.3171403843852	21.50879314692315	44.31064439384495
5	16.453807305160815	34.65327409030434	26.893683690931315	21.999234913603534
6	17.350765897015506	29.020334119921255	29.068039912811887	24.56086007025135
7	19.8917978805934	26.09820770405437	36.48275502851137	17.527239386840858
8	21.54901364909992	27.92216953390095	33.42389270301436	17.104924113984765
9	20.726287064693263	28.15766298875585	27.302304676168042	23.813745270382842
10-11	19.774171883661335	30.32763841837532	28.331135463410206	21.567054234553137
12-13	24.31674300858325	26.493565582012398	27.34804428670526	21.841647122699094
14-15	21.850995112484604	27.4773466472624	28.565490602165767	22.10616763808723
16-17	20.713782834814122	29.915705968122317	28.229411397441684	21.14109979962187
18-19	21.399842498445164	29.505825936290865	29.80192609982894	19.29240546543503
20-21	22.75085123622853	28.833357076997423	30.016774639973036	18.399017046801003
22-23	21.69359704093005	30.586294881130478	28.633806815339945	19.08630126259952
24-25	22.295955976832165	28.329376247620313	26.655861862236847	22.718805913310675
26-27	20.07432514240162	26.64487538715683	30.070396227137525	23.21040324330403
28-29	19.40552505474279	28.11873493452252	30.688524228254316	21.787215782480377
30-31	19.998796028411657	30.085352616197486	28.3279680702595	21.587883285131348
32-33	20.50159036556849	30.077433090297095	28.89993132159534	20.521045222539072
34-35	21.461484039945926	29.17128173357953	30.0034252966262	19.36380892984834
36-37	21.692717433035106	28.377237265433582	28.724734125578344	21.205311175952968
38-39	21.606067761103983	29.217778515198468	29.06484612676779	20.111307596929763
40-41	20.875884989064243	30.05586626866413	29.716781077735536	19.351467664536088
42-43	20.39301279917489	29.974560324799977	29.437291631812872	20.19513524421226
44-45	20.440552476544653	30.270860591117206	28.868955325922318	20.419631606415827
46-47	19.75135975135975	28.362494577810892	28.93371519342662	22.95243047740273
48-49	20.76269951364574	27.98266033831795	30.365208086805932	20.88943206123038
50-51	21.746080311995193	30.83099834806188	28.13317194540636	19.289749394536564
52-53	20.467898085744462	30.50984033727655	28.19872189793259	20.823539679046398
54-55	22.47372102671498	28.57230588233265	28.139421953152834	20.814551137799544
56-57	21.520237141400823	28.718948284442035	29.385076202460247	20.375738371696894
58-59	21.205751847929754	28.134848028251056	28.96271848833645	21.696681635482733
60-61	19.594548213478173	30.576199554814	29.541726787703265	20.28752544400456
62-63	20.361699118219327	31.364034664053985	27.403266314322384	20.870999903404307
64-65	19.74718658799858	30.316895511596954	28.671060917059606	21.264856983344863
66-67	20.19355513020094	30.605439288255788	29.2342340633159	19.96677151822737
68-69	20.77442403792455	30.15442465192536	29.13426921313727	19.936882097012816
70-71	19.580623652345846	31.137032906648766	29.05762259578154	20.22472084522385
72-73	20.01875462009735	30.885378812625923	28.65253728933822	20.443329277938503
74-75	19.929396806298957	29.52881647205486	30.24759409993518	20.294192621710998
76-77	19.420672993174588	30.762457921110553	28.669336075410335	21.14753301030452
78-79	19.30718632751286	29.716328178601103	28.601623514713083	22.374861979172955
80-81	21.00894701212913	29.422536122214034	28.2340107191673	21.334506146489538
82-83	20.316820965988843	28.38808576280459	29.779004552919453	21.516088718287115
84-85	20.54632963771538	28.841566750683594	28.727459185331313	21.884644426269713
86-87	21.00006933379878	28.066270003749544	29.31577888934153	21.617881773110145
88-89	20.907020616284335	27.727965908467656	28.95658841835807	22.408425056889932
90-91	20.54867525876857	28.447881852447328	28.910555605189202	22.092887283594898
92-93	20.473632632280093	28.80631344603123	29.213330440400703	21.50672348128798
94-95	20.93601318239032	29.177128538998883	28.964004720217424	20.922853558393374
96-97	19.991883461267417	29.32002170389363	29.53395614171552	21.154138693123432
98-99	21.70798121709447	29.49749553210931	28.149022134729023	20.645501116067194
100	20.668646876753826	31.0233910160644	27.43597058177266	20.87199152540911
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	6.0
2	10.5
3	14.5
4	20.0
5	23.5
6	37.5
7	47.5
8	44.5
9	50.0
10	64.5
11	74.0
12	87.5
13	106.5
14	128.0
15	163.5
16	227.5
17	319.0
18	460.5
19	730.5
20	1159.5
21	1826.0
22	2881.0
23	4597.5
24	7152.5
25	10617.0
26	15116.5
27	20557.5
28	26821.0
29	33762.5
30	41484.5
31	50627.5
32	61290.5
33	72653.5
34	85192.0
35	99287.0
36	113536.0
37	124066.5
38	130918.5
39	137200.5
40	141837.5
41	143735.5
42	142374.0
43	139892.0
44	138104.0
45	132799.0
46	122375.0
47	112514.0
48	105990.5
49	101580.0
50	97695.0
51	90206.0
52	78590.5
53	66058.5
54	54754.0
55	44901.0
56	34970.5
57	25778.5
58	18428.5
59	13003.0
60	9931.0
61	8016.0
62	6349.5
63	4893.5
64	3633.5
65	2808.5
66	2407.0
67	2197.5
68	1820.5
69	1569.5
70	1735.5
71	1660.5
72	1069.5
73	517.5
74	254.0
75	160.0
76	115.0
77	91.0
78	91.0
79	66.5
80	41.5
81	41.5
82	41.0
83	32.5
84	22.0
85	20.0
86	19.5
87	17.0
88	15.5
89	15.5
90	13.0
91	16.0
92	14.0
93	12.0
94	14.0
95	13.5
96	18.0
97	20.0
98	48.5
99	101.5
100	103.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.279194789685752
2	0.0053811306514376074
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	2.414609907696362E-4
30-31	0.009641192417159047
32-33	0.0
34-35	0.0
36-37	0.0
38-39	8.796078949465319E-4
40-41	0.0014660131582442198
42-43	1.3797770901122068E-4
44-45	0.0
46-47	0.0017764630035194664
48-49	1.7247213626402584E-5
50-51	0.0
52-53	0.0011383160993425707
54-55	0.019023676629922055
56-57	0.011262430498040888
58-59	0.015091311923102263
60-61	2.0696656351683104E-4
62-63	0.011693610838700954
64-65	1.207304953848181E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	2.2421377714323363E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2899019.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.27819069756961
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.2093282999644	62.439854083766065
2	9.138329166058556	13.392804545752025
3	2.5815754938545776	5.675195440165446
4	1.1105743213658785	3.2552350761949125
5	0.5681192284450852	2.0815374580477535
6	0.34053792182910186	1.4972401665328188
7	0.24153171380913246	1.238930488881149
8	0.15432091773917675	0.9046686110972278
9	0.11311324683661224	0.745986066490308
>10	0.5104917838724242	6.27591818691105
>50	0.022620317720978236	1.1027380909435767
>100	0.009074133567923654	1.1902843384863069
>500	3.3552306919764306E-4	0.16227105472922917
>1k	4.79318669845037E-5	0.03733639200212006
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	1.7247213626402588E-5	0.0	0.0	0.0	0.0
12-13	3.4494427252805176E-5	0.0	0.0	0.0	0.0
14-15	3.4494427252805176E-5	0.0	0.0	0.0	0.0
16-17	3.4494427252805176E-5	0.0	0.0	0.0	0.0
18-19	3.4494427252805176E-5	0.0	0.0	0.0	0.0
20-21	3.4494427252805176E-5	0.0	0.0	0.0	0.0
22-23	3.4494427252805176E-5	1.7247213626402588E-5	0.0	0.0	0.0
24-25	3.4494427252805176E-5	3.4494427252805176E-5	0.0	0.0	0.0
26-27	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
28-29	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
30-31	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
32-33	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
34-35	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
36-37	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
38-39	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
40-41	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
42-43	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
44-45	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
46-47	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
48-49	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
50-51	6.898885450561035E-5	3.4494427252805176E-5	0.0	0.0	0.0
52-53	6.898885450561035E-5	3.4494427252805176E-5	0.0	1.7247213626402588E-5	0.0
54-55	6.898885450561035E-5	3.4494427252805176E-5	0.0	3.4494427252805176E-5	0.0
56-57	8.623606813201294E-5	3.4494427252805176E-5	2.2421377714323363E-4	3.4494427252805176E-5	0.0
58-59	1.379777090112207E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
60-61	1.379777090112207E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
62-63	1.8971934989042846E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
64-65	2.414609907696362E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
66-67	2.759554180224414E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
68-69	2.93202631648844E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
70-71	3.6219148615445436E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
72-73	4.656747679128698E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
74-75	5.346636224184801E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
76-77	5.86405263297688E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
78-79	6.898885450561034E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
80-81	8.623606813201294E-4	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
82-83	0.001086574458463363	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
84-85	0.0011383160993425707	3.4494427252805176E-5	4.4842755428646725E-4	3.4494427252805176E-5	0.0
86-87	0.0011383160993425707	1.0348328175841552E-4	4.4842755428646725E-4	3.4494427252805176E-5	0.0
88	0.0011383160993425707	1.0348328175841552E-4	4.4842755428646725E-4	3.4494427252805176E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACCGGC	11145	0.0	80.82001	9
TCACCGG	11590	0.0	80.63281	8
GCAGCCT	70	0.0	75.576416	1
CTTCACC	13065	0.0	73.97261	6
CGTAGTG	2490	0.0	71.06667	4
CCTTCAC	14660	0.0	70.759094	5
TTCACCG	13970	0.0	69.079735	7
GTGTGGC	4170	0.0	68.09941	7
CGGGCCC	870	0.0	67.97908	7
ATGCAGC	85	0.0	66.26647	2
GCGTAGT	1590	0.0	64.355156	3
ACGGGCC	920	0.0	63.77437	6
GGGCCCG	930	0.0	63.088623	8
GGCCCGA	955	0.0	60.9456	9
AACGGGC	950	0.0	60.772278	5
CGGGGCA	1070	0.0	60.098103	94
ACCTTCA	5645	0.0	59.950844	4
CAGCGTA	1160	0.0	58.832336	1
GCCTTCA	4630	0.0	57.582607	4
AGCGTAG	1475	0.0	57.28118	2
>>END_MODULE