FastQCFastQC Report
Tue 7 Mar 2017
1HL_34A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_34A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2899019
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGGC111450.080.820019
TCACCGG115900.080.632818
GCAGCCT700.075.5764161
CTTCACC130650.073.972616
CGTAGTG24900.071.066674
CCTTCAC146600.070.7590945
TTCACCG139700.069.0797357
GTGTGGC41700.068.099417
CGGGCCC8700.067.979087
ATGCAGC850.066.266472
GCGTAGT15900.064.3551563
ACGGGCC9200.063.774376
GGGCCCG9300.063.0886238
GGCCCGA9550.060.94569
AACGGGC9500.060.7722785
CGGGGCA10700.060.09810394
ACCTTCA56450.059.9508444
CAGCGTA11600.058.8323361
GCCTTCA46300.057.5826074
AGCGTAG14750.057.281182
Produced by FastQC (version 0.11.5)