FastQCFastQC Report
Mon 6 Mar 2017
1HL_35A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_35A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2630313
Sequences flagged as poor quality0
Sequence length92
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA131860.5013091597844059No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG73040.2776855834267633No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA67710.2574218353481125No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG56170.21354872975193448No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG49470.18807647606957803No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT49150.18685989081907742No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA47370.1800926353631678No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG47010.17872397695635464No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA45360.17245095925846088No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT42230.1605512347770018No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC40910.15553282061868684No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT39750.15112269908562215No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA35280.13412852386769178No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC34750.13211355454655016No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA34570.13142922534314358No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32270.12268501885517047No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA28970.11013898345938297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT450.086.03
GCTGCTA500.086.03
AGCTGCT1500.086.02
GCAGCCG258.359184E-786.03
AGCAGCC1400.086.02
GCAGCCC159.829662E-485.999993
GCAGCAC600.085.999993
CAGCTGC6100.085.295081
AGCTGCA1750.083.5428542
CAGCAGC6150.083.203251
AGCTGCC2350.082.340432
GCTGCAA950.081.473693
GCTGCCC1700.080.941183
CAGCCCT157250.080.8317951
AGCAGCA2400.080.624992
GCTGCAT700.079.857143
GCTGCAG700.079.857143
GCTGCCA650.079.384613
CTGCTGC1900.079.2105261
AGCAGCG1250.079.1199952
Produced by FastQC (version 0.11.5)