FastQCFastQC Report
Tue 7 Mar 2017
1HL_35A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_35A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2630313
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG104000.080.473168
CACCGGC100000.079.8430949
CTTCACC116650.074.361866
CGGGCCC7750.072.67967
CCTTCAC126200.072.1564945
GGGCCCG7950.070.2607658
GTGTGGC41800.069.1731347
CGTAGTG21650.067.427264
TTCACCG128750.066.753527
CGGGGCA10400.065.4436894
AACGGGC8750.063.8369265
ACGGGCC8900.063.8158236
GCTGCCA1400.063.7064442
GCCTTCA37000.063.177244
ACCTTCA50950.061.3568534
GCGTAGT15050.061.129753
GGCCCGA9250.060.3862769
AGCTGCT950.059.2946432
CAGCGTA10000.059.2937851
TGTGGCA51000.058.4435468
Produced by FastQC (version 0.11.5)