##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_35A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2630313 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.827835318458298 12.0 12.0 12.0 2.0 32.0 2 14.672894822783448 12.0 12.0 12.0 12.0 22.0 3 13.713491892409763 12.0 12.0 12.0 12.0 22.0 4 13.775938833135069 12.0 12.0 12.0 12.0 22.0 5 22.430861270122605 22.0 22.0 22.0 22.0 27.0 6 26.813030996691268 27.0 22.0 32.0 22.0 32.0 7 34.947662122340574 37.0 37.0 37.0 27.0 41.0 8 37.5603705718673 41.0 37.0 41.0 27.0 41.0 9 38.517884753639585 41.0 37.0 41.0 32.0 41.0 10-11 38.88065431756601 41.0 41.0 41.0 37.0 41.0 12-13 39.06373709136517 41.0 41.0 41.0 37.0 41.0 14-15 39.017354588598394 41.0 41.0 41.0 37.0 41.0 16-17 38.92259305261389 41.0 41.0 41.0 37.0 41.0 18-19 38.85898218196846 41.0 41.0 41.0 37.0 41.0 20-21 38.87243400310153 41.0 41.0 41.0 37.0 41.0 22-23 38.85452396729971 41.0 41.0 41.0 34.5 41.0 24-25 38.880557180837414 41.0 41.0 41.0 37.0 41.0 26-27 38.84684275217436 41.0 41.0 41.0 37.0 41.0 28-29 38.796487528290356 41.0 41.0 41.0 34.5 41.0 30-31 38.67646378967066 41.0 41.0 41.0 32.0 41.0 32-33 38.71526411495514 41.0 41.0 41.0 32.0 41.0 34-35 38.68751684685435 41.0 41.0 41.0 32.0 41.0 36-37 38.60747085993188 41.0 41.0 41.0 32.0 41.0 38-39 38.51389492429228 41.0 41.0 41.0 32.0 41.0 40-41 38.56382111178404 41.0 41.0 41.0 32.0 41.0 42-43 38.29727089513682 41.0 41.0 41.0 32.0 41.0 44-45 38.412722744403425 41.0 41.0 41.0 32.0 41.0 46-47 38.43633039109794 41.0 41.0 41.0 32.0 41.0 48-49 38.37122977379498 41.0 41.0 41.0 32.0 41.0 50-51 38.24852365478937 41.0 41.0 41.0 32.0 41.0 52-53 38.23328421370384 41.0 41.0 41.0 32.0 41.0 54-55 38.086178146859325 41.0 37.0 41.0 32.0 41.0 56-57 37.88270198261576 41.0 37.0 41.0 29.5 41.0 58-59 37.876543590059434 41.0 37.0 41.0 29.5 41.0 60-61 37.869823667373424 41.0 37.0 41.0 29.5 41.0 62-63 37.87356979948774 41.0 37.0 41.0 29.5 41.0 64-65 37.85922720984156 41.0 37.0 41.0 29.5 41.0 66-67 37.72556517038086 41.0 37.0 41.0 27.0 41.0 68-69 37.75817459747186 41.0 37.0 41.0 27.0 41.0 70-71 37.73421813297505 41.0 37.0 41.0 27.0 41.0 72-73 37.58578807921339 41.0 37.0 41.0 27.0 41.0 74-75 37.52146417555629 41.0 37.0 41.0 27.0 41.0 76-77 36.44943510525173 39.0 34.5 41.0 24.5 41.0 78-79 36.97771824113708 41.0 37.0 41.0 27.0 41.0 80-81 37.61423868566212 41.0 37.0 41.0 27.0 41.0 82-83 37.799000537198424 41.0 37.0 41.0 27.0 41.0 84-85 37.71877795532319 41.0 37.0 41.0 27.0 41.0 86-87 37.58446827430804 41.0 37.0 41.0 27.0 41.0 88-89 37.497864892885374 41.0 37.0 41.0 27.0 41.0 90-91 37.32980428564966 41.0 37.0 41.0 27.0 41.0 92-93 37.212038073035416 41.0 37.0 41.0 27.0 41.0 94-95 37.05993393181724 41.0 37.0 41.0 27.0 41.0 96-97 37.00528435209041 41.0 37.0 41.0 27.0 41.0 98-99 36.87988349675495 41.0 37.0 41.0 27.0 41.0 100 36.75439044706847 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.425656569663719 1101 2 -0.6406663986029191 1101 3 -7.011936337892877E-4 1101 4 -0.09223323220605195 1101 5 -1.0888792736889634 1101 6 -0.8027610259026368 1101 7 -0.3166968419905132 1101 8 0.10194597641766734 1101 9 0.0773896303119912 1101 10-11 -0.17282271190617138 1101 12-13 -0.10605347842869861 1101 14-15 0.01939360454098704 1101 16-17 -0.07606195127092263 1101 18-19 0.016267011885624072 1101 20-21 -0.13633230290921006 1101 22-23 0.02207599776343727 1101 24-25 -0.14561338932992385 1101 26-27 0.06930142346508461 1101 28-29 -0.09182913126057457 1101 30-31 -0.18301088927742626 1101 32-33 -0.1566712601119491 1101 34-35 -0.016276244613315782 1101 36-37 0.024363657488137846 1101 38-39 -0.08979863889507556 1101 40-41 -0.02109450733897944 1101 42-43 0.08141737476863398 1101 44-45 -0.0952129311754959 1101 46-47 0.016612914831860337 1101 48-49 0.03731083402951185 1101 50-51 -0.06394866670469668 1101 52-53 -0.06343207926228445 1101 54-55 0.06347074311435819 1101 56-57 0.175109532579242 1101 58-59 -0.052920267601102466 1101 60-61 0.08018874737530979 1101 62-63 -0.054737542599966105 1101 64-65 0.0404306597826789 1101 66-67 -0.10486871517501584 1101 68-69 -0.1326673591819585 1101 70-71 -0.04892110272886896 1101 72-73 -0.04525453883657349 1101 74-75 0.08527263441119715 1101 76-77 -0.22213773181746177 1101 78-79 -0.05455718555951705 1101 80-81 0.0027055610407202835 1101 82-83 -0.006693989747141416 1101 84-85 0.02079470044225218 1101 86-87 -0.07605647367258683 1101 88-89 -0.21689544197494826 1101 90-91 -0.20095677532079748 1101 92-93 -0.06713888798287115 1101 94-95 -0.10284270928203654 1101 96-97 -0.2410694887439604 1101 98-99 -0.3767121632064274 1101 100 -0.6268214874130678 1102 1 1.6955021818821638 1102 2 0.8517503541552394 1102 3 -0.1185520707381329 1102 4 0.018866589890926377 1102 5 -0.7605477901820592 1102 6 -0.1692076302845642 1102 7 -0.15438325387780338 1102 8 -0.2374859354858856 1102 9 -0.28858222305240844 1102 10-11 -0.3200675483917479 1102 12-13 -0.3993422187272202 1102 14-15 -0.2710909997648727 1102 16-17 -0.328309439858252 1102 18-19 -0.2647585825957961 1102 20-21 -0.2202273667663519 1102 22-23 -0.18868433326147738 1102 24-25 -0.15552427613873476 1102 26-27 -0.2613489725680225 1102 28-29 -0.48658037660929665 1102 30-31 -0.38411011058654054 1102 32-33 -0.2289299634059745 1102 34-35 -0.15993325684569015 1102 36-37 -0.2943493410073188 1102 38-39 -0.25848452844601155 1102 40-41 -0.21962421225659767 1102 42-43 -0.2351144147175077 1102 44-45 -0.3069383810452635 1102 46-47 -0.29072048182610644 1102 48-49 -0.3700255390086724 1102 50-51 -0.21127278146583706 1102 52-53 -0.3710866310203471 1102 54-55 -0.3872872509009042 1102 56-57 -0.13833187397720792 1102 58-59 -0.261699284781983 1102 60-61 -0.2931896995531744 1102 62-63 -0.25725559701003675 1102 64-65 -0.34100909249429634 1102 66-67 -0.21237483374922306 1102 68-69 -0.4231609661193474 1102 70-71 -0.4579010072017695 1102 72-73 -0.325844148734177 1102 74-75 -0.3749890151317885 1102 76-77 -0.2360848290475417 1102 78-79 -0.4028444716574455 1102 80-81 -0.4648836793815363 1102 82-83 -0.34455815767524456 1102 84-85 -0.525859783887924 1102 86-87 -0.2630636370113706 1102 88-89 -0.35636990509200217 1102 90-91 -0.5100337428596973 1102 92-93 -0.36878583674300813 1102 94-95 -0.537348473079085 1102 96-97 -0.2870618203391615 1102 98-99 -0.2491122629335436 1102 100 -0.6291357148963357 1103 1 1.6153669294170694 1103 2 0.8656933954376775 1103 3 0.38851578329567893 1103 4 0.8893099201735435 1103 5 2.795253303607712 1103 6 -0.1645878283017197 1103 7 -0.42751009449960264 1103 8 -0.2999466359489986 1103 9 -0.22435525126825695 1103 10-11 -0.11090149769231772 1103 12-13 -0.3586882858902172 1103 14-15 -0.29246336012012364 1103 16-17 -0.23935638811375526 1103 18-19 -0.20518377852302905 1103 20-21 -0.12872887936291733 1103 22-23 -0.004378503668661438 1103 24-25 -0.13057774642270203 1103 26-27 -0.021869027767344562 1103 28-29 -0.1179405525566466 1103 30-31 -0.0155010292022979 1103 32-33 -0.38433415216137945 1103 34-35 -0.2803372040515555 1103 36-37 -0.1802911770244009 1103 38-39 -0.10628836578025158 1103 40-41 -0.2021364076440122 1103 42-43 0.03989479376189564 1103 44-45 -0.47961546215682915 1103 46-47 -0.48193376892196227 1103 48-49 -0.3345493589437112 1103 50-51 -0.20761335371101097 1103 52-53 -0.24122157987772397 1103 54-55 -0.2742388212688738 1103 56-57 -0.06598501877078888 1103 58-59 -0.42066618600311756 1103 60-61 -0.23039512607332568 1103 62-63 -0.12215245344807357 1103 64-65 -0.1882984197664257 1103 66-67 -0.30353629748977085 1103 68-69 -0.346941584910077 1103 70-71 -0.3950893438534564 1103 72-73 -0.3595942520849178 1103 74-75 -0.34495753349365543 1103 76-77 -0.3562467493453525 1103 78-79 -0.42962534574598266 1103 80-81 -0.1878993021945803 1103 82-83 -0.23450188713518116 1103 84-85 -0.33349345577887846 1103 86-87 -0.18063967881070653 1103 88-89 -0.03793505443930911 1103 90-91 -0.4725091654329532 1103 92-93 -0.2020728333007824 1103 94-95 -0.1861689570908851 1103 96-97 -0.24171361570145677 1103 98-99 -0.15727426953996826 1103 100 -0.12420342610085555 1104 1 1.868895375926284 1104 2 -0.5769234752246124 1104 3 0.025109627593979056 1104 4 0.04898426806722611 1104 5 -1.147132831477169 1104 6 -0.508852921691112 1104 7 -0.32702812831659145 1104 8 -0.10564615676115352 1104 9 -0.11165181622921949 1104 10-11 -0.053525504506481525 1104 12-13 0.06984315304535471 1104 14-15 0.05082132114984006 1104 16-17 0.05850211931154803 1104 18-19 0.01959120909337031 1104 20-21 0.10437885007911518 1104 22-23 0.07684917325889273 1104 24-25 0.05763787878127147 1104 26-27 0.004899153672837997 1104 28-29 0.04861742258784574 1104 30-31 -0.023780012017112995 1104 32-33 -0.04156907789687381 1104 34-35 0.01914929748900107 1104 36-37 0.002229228088282298 1104 38-39 -0.18606074346804036 1104 40-41 -0.09825753801808901 1104 42-43 -0.029481542720418474 1104 44-45 -0.017330405430136864 1104 46-47 -0.13248012222464922 1104 48-49 -0.27305685275952385 1104 50-51 -0.04178640596496308 1104 52-53 -0.0691869411867998 1104 54-55 -0.0769375426417227 1104 56-57 -0.1600271312011472 1104 58-59 -0.11721093738243837 1104 60-61 0.0770413341060916 1104 62-63 -0.022419497766243524 1104 64-65 -0.13375452738949178 1104 66-67 -0.37156152254973307 1104 68-69 -0.4285518547270257 1104 70-71 -0.17887707245057527 1104 72-73 -0.25893093249549537 1104 74-75 -0.265495721135629 1104 76-77 -0.3239146530084511 1104 78-79 -0.05309713882117251 1104 80-81 -0.024557067383973674 1104 82-83 -0.3594594270315028 1104 84-85 -0.1798336469858839 1104 86-87 -0.17185945268794 1104 88-89 -0.24794981580733833 1104 90-91 -0.14871350524089877 1104 92-93 -0.21502428291965003 1104 94-95 -0.1534810997956555 1104 96-97 -0.1256569504689935 1104 98-99 -0.23209054217370095 1104 100 -0.18172347440594194 1105 1 1.6386666547883895 1105 2 0.3140337449478121 1105 3 -0.11967366298068072 1105 4 -0.15972127726452356 1105 5 -1.0717427907733281 1105 6 0.3107592586386865 1105 7 0.05050117572931612 1105 8 -0.14154404360628803 1105 9 -0.06832624895909589 1105 10-11 -0.1424443597222833 1105 12-13 -0.05522207282016467 1105 14-15 -0.11564895779385864 1105 16-17 -0.11698954501320458 1105 18-19 -0.12065972238094247 1105 20-21 -0.03047424441000146 1105 22-23 -0.08624930465315117 1105 24-25 -0.009928140310549338 1105 26-27 0.020418467600073598 1105 28-29 -0.12393667544586151 1105 30-31 0.022838940198937507 1105 32-33 -0.10432379419381732 1105 34-35 0.011868567902105553 1105 36-37 0.035718154649124756 1105 38-39 -0.009454482872804704 1105 40-41 0.053717459488915154 1105 42-43 -0.0032088904436307075 1105 44-45 -0.022294279638039427 1105 46-47 -0.07434860077128036 1105 48-49 -0.22976274257811724 1105 50-51 -0.12054779442827623 1105 52-53 -0.17131831759307659 1105 54-55 0.0011328777228882814 1105 56-57 -0.00924179183627416 1105 58-59 -0.15626015367485024 1105 60-61 -0.09947934870310604 1105 62-63 -0.16689583242614958 1105 64-65 -0.22169041657351585 1105 66-67 -0.2728654052791555 1105 68-69 -0.29088312416106277 1105 70-71 -0.3270201070171055 1105 72-73 -0.15507295173237168 1105 74-75 -0.36856410177546195 1105 76-77 -0.3808721995368387 1105 78-79 -0.22726325400670078 1105 80-81 -0.26422345906552636 1105 82-83 -0.39190954442899084 1105 84-85 -0.23368655110068914 1105 86-87 -0.24640840154977894 1105 88-89 -0.1027219558865724 1105 90-91 -0.33957073357221645 1105 92-93 -0.3343623242399403 1105 94-95 -0.1341940062674709 1105 96-97 -0.07059961153926508 1105 98-99 -0.3107904669305199 1105 100 -0.19137817166546256 1106 1 1.7193915015247754 1106 2 -0.8129767130399266 1106 3 -0.15612335645345254 1106 4 -0.34239347344194826 1106 5 -1.0817139411519783 1106 6 -0.6778992339685956 1106 7 -0.21551397032188646 1106 8 0.01685660551019197 1106 9 -0.010437680621556922 1106 10-11 -0.02880389812834494 1106 12-13 -0.03386343596477559 1106 14-15 -0.011207919207485872 1106 16-17 -0.00912081079034266 1106 18-19 0.047251384723274725 1106 20-21 -0.008581889559664546 1106 22-23 0.00500475232485087 1106 24-25 -0.13835673749771615 1106 26-27 -0.26478798750270016 1106 28-29 -0.252587054097134 1106 30-31 0.02709696399988104 1106 32-33 -0.09646407547762692 1106 34-35 -0.05103803144877617 1106 36-37 -0.39578112411034283 1106 38-39 -0.15734305121033998 1106 40-41 0.00306113060323554 1106 42-43 0.180776865055833 1106 44-45 0.05319977012755572 1106 46-47 -0.05064142442797248 1106 48-49 -0.22183721066322448 1106 50-51 -0.05854635194720714 1106 52-53 -0.2004176684765966 1106 54-55 -0.2617466751079789 1106 56-57 -0.17860169086259248 1106 58-59 -0.19276934949165536 1106 60-61 -0.057804267563547285 1106 62-63 -0.14611328644706845 1106 64-65 -0.45570952944765253 1106 66-67 -0.4355916944966083 1106 68-69 -0.3828908774968056 1106 70-71 -0.4092117766889416 1106 72-73 -0.31318780928736345 1106 74-75 -0.4773938890095053 1106 76-77 -0.23705773866414148 1106 78-79 -0.18364442962877092 1106 80-81 -0.42686049476693455 1106 82-83 -0.5458790720164473 1106 84-85 -0.616585938046228 1106 86-87 -0.4145106373954306 1106 88-89 -0.44121095696843327 1106 90-91 -0.5869463195801572 1106 92-93 -0.3011510007635607 1106 94-95 -0.3221646157157281 1106 96-97 -0.35249286794027057 1106 98-99 -0.5494327316257923 1106 100 -0.6195696610271639 1107 1 1.3213653041085323 1107 2 -0.5330458051267222 1107 3 -0.2568331696889068 1107 4 -0.09732348171860394 1107 5 -1.1637877833197443 1107 6 -0.22283607948300954 1107 7 -0.28703507579528065 1107 8 -0.2757636758961226 1107 9 -0.2025901510235144 1107 10-11 -0.15163937259665516 1107 12-13 -0.43294895541492195 1107 14-15 -0.3349736178652236 1107 16-17 -0.3033865274408143 1107 18-19 -0.2398167897342418 1107 20-21 -0.36111841480346385 1107 22-23 -0.16111988332234262 1107 24-25 -0.31854629091813536 1107 26-27 -0.27163826998857843 1107 28-29 -0.24232624946220938 1107 30-31 -0.27587678460568554 1107 32-33 -0.12830491177976455 1107 34-35 -0.39194450523336144 1107 36-37 -0.3271986814171868 1107 38-39 -0.2865281604262222 1107 40-41 -0.23971118937949853 1107 42-43 -0.32227335265621093 1107 44-45 -0.20293845003150324 1107 46-47 -0.2835951111385384 1107 48-49 -0.42982553338084983 1107 50-51 -0.18236872770739865 1107 52-53 -0.3035209647449051 1107 54-55 -0.4514989152029827 1107 56-57 -0.19962169877939573 1107 58-59 -0.5756150798166004 1107 60-61 -0.3645848282170405 1107 62-63 -0.5310047902795176 1107 64-65 -0.5383114582684101 1107 66-67 -0.5385838856513772 1107 68-69 -0.5935664145798185 1107 70-71 -0.5541904553027877 1107 72-73 -0.5338736022270183 1107 74-75 -0.33169325206684164 1107 76-77 -0.4069014717959547 1107 78-79 -0.7356623144033207 1107 80-81 -0.5148229079520021 1107 82-83 -0.3506925773602845 1107 84-85 -0.2767104477443141 1107 86-87 -0.33116875751489516 1107 88-89 -0.2092684617365066 1107 90-91 -0.11596274694349518 1107 92-93 -0.4107150368929666 1107 94-95 -0.2558735473073668 1107 96-97 -0.4343507973367977 1107 98-99 -0.569749223762976 1107 100 -0.4260983234475475 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30-31 0.08497133187401573 2223 32-33 0.17698610482493393 2223 34-35 0.2149879150507914 2223 36-37 0.20276960954684853 2223 38-39 0.16760965048315057 2223 40-41 0.22977222685705812 2223 42-43 0.40731610486891157 2223 44-45 0.29530859890768824 2223 46-47 0.24847199904478856 2223 48-49 0.11842191648742073 2223 50-51 0.13422234155265045 2223 52-53 0.28725249873892267 2223 54-55 0.2002701175689694 2223 56-57 0.33730672112343996 2223 58-59 0.39655830330320896 2223 60-61 0.23865138227251492 2223 62-63 0.43048329811733055 2223 64-65 0.39067179447817324 2223 66-67 0.36710004145793107 2223 68-69 0.44209783136611946 2223 70-71 0.23629977405538227 2223 72-73 0.236367035410602 2223 74-75 0.3263933539615351 2223 76-77 0.312382949970754 2223 78-79 0.43201219889104436 2223 80-81 0.28593716450033213 2223 82-83 0.2846474505978378 2223 84-85 0.10189027410623197 2223 86-87 0.19268928507802485 2223 88-89 0.27488931544136364 2223 90-91 0.32582537896249164 2223 92-93 0.3584827396434136 2223 94-95 0.536361970911301 2223 96-97 0.49986422360506566 2223 98-99 0.46289985726301097 2223 100 0.5949183792581891 2224 1 1.1108392265798877 2224 2 -0.9546774105210432 2224 3 -0.03736635453809001 2224 4 -0.3353915580792943 2224 5 -1.1285590136105093 2224 6 0.5118752591830784 2224 7 0.25173694218578646 2224 8 0.148045395217828 2224 9 0.08655285098192422 2224 10-11 0.07809050395325556 2224 12-13 0.07081068134215229 2224 14-15 0.04682847268293955 2224 16-17 0.11966925823498542 2224 18-19 0.03482637297813085 2224 20-21 0.0318571079467489 2224 22-23 0.06484673490589188 2224 24-25 0.16951567405228474 2224 26-27 0.1836566838138438 2224 28-29 -0.020180834589353935 2224 30-31 -0.01576424448553837 2224 32-33 0.17327771195325425 2224 34-35 0.030410054995520852 2224 36-37 0.11063037167947698 2224 38-39 0.0911888052458778 2224 40-41 0.15649974865100802 2224 42-43 0.3434422006056792 2224 44-45 0.21349123050123353 2224 46-47 0.25647252158048417 2224 48-49 0.15793357824935583 2224 50-51 0.16904209805459658 2224 52-53 0.18831376515427678 2224 54-55 0.32456480622627026 2224 56-57 0.40668344520042865 2224 58-59 0.270000337183923 2224 60-61 0.37038137805821947 2224 62-63 0.2712604983818139 2224 64-65 0.3591806082416227 2224 66-67 0.20613972106480816 2224 68-69 0.29582013764539994 2224 70-71 0.31497825981136884 2224 72-73 0.1767289808539445 2224 74-75 0.27052721973249305 2224 76-77 0.33311455362743203 2224 78-79 0.3014274868180564 2224 80-81 0.21601607275864154 2224 82-83 0.2477211773420933 2224 84-85 0.13937156258619865 2224 86-87 0.3182443226710703 2224 88-89 0.22453476960451013 2224 90-91 0.40686985981343327 2224 92-93 0.21331811853100646 2224 94-95 0.5464265989133068 2224 96-97 0.31538505479471723 2224 98-99 0.33989092852382186 2224 100 0.4899719167979981 2225 1 1.403566962563044 2225 2 1.7001526619378673 2225 3 0.812789872413406 2225 4 1.0493469238149178 2225 5 3.195610793240231 2225 6 -0.1996470931165213 2225 7 0.36107345816832037 2225 8 0.4275919571073885 2225 9 0.3730404489310857 2225 10-11 0.30028948210830464 2225 12-13 0.27818352064965524 2225 14-15 0.3096898173522291 2225 16-17 0.2510380208907108 2225 18-19 0.19535526490663102 2225 20-21 0.19566028433793292 2225 22-23 0.1580938863901622 2225 24-25 0.3055560472089667 2225 26-27 0.17741833378500615 2225 28-29 0.23553832343901604 2225 30-31 0.2315061421258804 2225 32-33 0.2812826110874198 2225 34-35 0.2769174104017722 2225 36-37 0.1976954952976797 2225 38-39 0.23873856766469004 2225 40-41 0.2372934441447896 2225 42-43 0.4283308749339483 2225 44-45 0.2903182365312844 2225 46-47 0.5095387269418126 2225 48-49 0.458901783047466 2225 50-51 0.3758387526969287 2225 52-53 0.36416988437846953 2225 54-55 0.3283298981073841 2225 56-57 0.36899184927172257 2225 58-59 0.3374228830350816 2225 60-61 0.3255672635454303 2225 62-63 0.30025881318695014 2225 64-65 0.2282056537240038 2225 66-67 0.37666534988387923 2225 68-69 0.4182430634476546 2225 70-71 0.4962909238049633 2225 72-73 0.47447475333922284 2225 74-75 0.5011700522902771 2225 76-77 0.5922546125421988 2225 78-79 0.3813495297927574 2225 80-81 0.37744030827467867 2225 82-83 0.3994790099222243 2225 84-85 0.36872479641704103 2225 86-87 0.5866002072195684 2225 88-89 0.4331610005343194 2225 90-91 0.46888950105019944 2225 92-93 0.5699670392463858 2225 94-95 0.6998155392073429 2225 96-97 0.6717742677595453 2225 98-99 0.5380837023009306 2225 100 0.8889733159120397 2226 1 1.223006935087568 2226 2 1.119429141070098 2226 3 0.6608778583322241 2226 4 1.0688042473887336 2226 5 3.128819986076575 2226 6 -0.4278327099910406 2226 7 -0.05876612512029311 2226 8 0.11549720249058026 2226 9 0.15122570805201008 2226 10-11 0.1305441668916174 2226 12-13 0.29697765849927293 2226 14-15 0.17377573903281274 2226 16-17 0.024332072879467148 2226 18-19 0.12052537674040309 2226 20-21 0.12246962313773935 2226 22-23 0.17466548003932303 2226 24-25 0.1688922067956895 2226 26-27 0.048427145256020765 2226 28-29 0.21820024271549698 2226 30-31 0.15324450261270783 2226 32-33 0.19924729723018686 2226 34-35 0.17171316887517207 2226 36-37 0.14164945956525798 2226 38-39 0.1767700106280401 2226 40-41 0.04353374007855848 2226 42-43 0.23940039718980444 2226 44-45 0.2393526595820603 2226 46-47 0.33163716642064145 2226 48-49 0.17735220913199612 2226 50-51 0.11055542973844723 2226 52-53 0.38644818447649243 2226 54-55 0.2133928806275449 2226 56-57 0.26196708914550015 2226 58-59 0.38610205676738474 2226 60-61 0.41421010740030084 2226 62-63 0.23612119611208016 2226 64-65 0.33025969598185867 2226 66-67 0.3366569605621095 2226 68-69 0.24379391454345978 2226 70-71 0.447034246334141 2226 72-73 0.2559725424220147 2226 74-75 0.35276822703144717 2226 76-77 0.532036812154395 2226 78-79 0.39028612512335314 2226 80-81 0.47489515977115815 2226 82-83 0.35609423162213005 2226 84-85 0.34578679869235884 2226 86-87 0.3809093313672278 2226 88-89 0.20500006865776044 2226 90-91 0.16507253852130788 2226 92-93 0.15995199570777885 2226 94-95 0.5938206622388051 2226 96-97 0.5158171337951387 2226 98-99 0.46178523563283846 2226 100 0.4892187348772694 2227 1 1.2787135354230816 2227 2 1.7738876937397343 2227 3 0.7612495249024782 2227 4 -0.07120703276398288 2227 5 3.064472499525987 2227 6 0.09201602026869082 2227 7 -0.0032258347286671096 2227 8 0.07067016483222233 2227 9 0.19422333632759603 2227 10-11 0.09174694932928418 2227 12-13 -0.13640580884613485 2227 14-15 -0.08959774971596346 2227 16-17 0.00698088412490705 2227 18-19 0.02394629997751707 2227 20-21 -0.05815816074610325 2227 22-23 -0.0017749086920986201 2227 24-25 0.01164326250228953 2227 26-27 0.06550862266679047 2227 28-29 0.1658451492926929 2227 30-31 0.1768850995930933 2227 32-33 0.013917280454577963 2227 34-35 0.0781992566868226 2227 36-37 0.13981209622863844 2227 38-39 0.021258643704918256 2227 40-41 0.03356960517950114 2227 42-43 0.39984466166599475 2227 44-45 0.09663197351738262 2227 46-47 0.1633416264194878 2227 48-49 0.06269668764186065 2227 50-51 0.08107229212058797 2227 52-53 0.0074213145742518805 2227 54-55 0.14778722319710624 2227 56-57 0.3457627159170613 2227 58-59 0.2932475996608872 2227 60-61 0.1036364104025509 2227 62-63 0.3801578999936126 2227 64-65 0.05806165699302568 2227 66-67 0.19555237330000352 2227 68-69 0.1226175970794614 2227 70-71 0.1265933080568189 2227 72-73 0.18469354664484428 2227 74-75 0.25862306014303016 2227 76-77 0.3358236172680691 2227 78-79 0.19580472291360707 2227 80-81 0.03987679336866279 2227 82-83 0.2556039788310116 2227 84-85 0.1933716890724142 2227 86-87 0.1265910575198248 2227 88-89 0.029339890489190168 2227 90-91 -0.038853099241173084 2227 92-93 0.09736594838897616 2227 94-95 0.30008114699614197 2227 96-97 0.28119357273769197 2227 98-99 0.4310757429504761 2227 100 0.30500661015927477 2228 1 1.3004175074763857 2228 2 1.4998710033528795 2228 3 -0.11450700533716685 2228 4 -0.06552496953889886 2228 5 3.205260800441728 2228 6 0.12416585997772245 2228 7 0.16719473139315255 2228 8 0.4592449409280235 2228 9 0.28005950889555464 2228 10-11 0.31754427110441696 2228 12-13 0.3554755974016217 2228 14-15 0.3393063775967562 2228 16-17 0.3217959385375835 2228 18-19 0.3062767365727339 2228 20-21 0.3312020770308095 2228 22-23 0.4612009326557853 2228 24-25 0.3257914561740307 2228 26-27 0.3101259369664717 2228 28-29 0.21817871263996835 2228 30-31 0.36378005509978095 2228 32-33 0.2320795768562931 2228 34-35 0.28325632889681884 2228 36-37 0.30222763798336416 2228 38-39 0.48227002220723847 2228 40-41 0.37270000871021836 2228 42-43 0.4653089663769663 2228 44-45 0.5084236024473867 2228 46-47 0.5128690196427002 2228 48-49 0.4517314916268447 2228 50-51 0.3198927159212772 2228 52-53 0.3358743989174897 2228 54-55 0.42491718826374125 2228 56-57 0.5227974408347649 2228 58-59 0.5124296844497778 2228 60-61 0.3304962887826761 2228 62-63 0.35004116791672857 2228 64-65 0.46871327931012985 2228 66-67 0.48344195941865564 2228 68-69 0.5400283947965718 2228 70-71 0.7267636621345375 2228 72-73 0.5390233794534538 2228 74-75 0.6695738802865066 2228 76-77 0.6102311516623402 2228 78-79 0.5954476622042932 2228 80-81 0.44902559801494846 2228 82-83 0.44626074052218456 2228 84-85 0.4182582993856627 2228 86-87 0.4212858218274178 2228 88-89 0.4691324826025962 2228 90-91 0.3916230296241423 2228 92-93 0.5617797362873702 2228 94-95 0.906396107965648 2228 96-97 0.4574264834204058 2228 98-99 0.5821032583685977 2228 100 0.6850316960551126 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 15.0 13 29.0 14 120.0 15 461.0 16 1754.0 17 4392.0 18 7172.0 19 9240.0 20 11026.0 21 12394.0 22 14446.0 23 16403.0 24 18439.0 25 20786.0 26 23755.0 27 26717.0 28 30258.0 29 33929.0 30 39411.0 31 45203.0 32 53099.0 33 63689.0 34 78024.0 35 100280.0 36 140505.0 37 233191.0 38 568988.0 39 1075242.0 40 1345.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.953724856617544 18.25184887900991 20.87518180071897 34.91924446365358 2 18.476231321024592 51.10763097192075 13.755573323782594 16.660564383272064 3 41.04944924805527 15.241303981693433 23.505833716367597 20.2034130538837 4 17.65538930157742 16.232858979140506 22.285712764982723 43.82603895429936 5 17.092490513486418 34.544177822183144 26.58185546739114 21.781476196939302 6 17.451383162384097 28.51455321096767 29.97536795050627 24.058695676141966 7 20.085328248006988 26.545738092766907 35.97754335700733 17.391390302218785 8 21.13957540414392 27.98199301756103 33.76149530493139 17.116936273363663 9 20.70829593284145 28.07190627123084 27.75004343589527 23.46975436003244 10-11 19.594169971406444 30.22187853688896 28.66980089441827 21.51415059728633 12-13 24.192196898239867 26.656884560886862 27.390067265758866 21.76085127511441 14-15 21.775963545022968 27.530316734168135 28.503717998580395 22.190001722228494 16-17 20.7200435841666 29.81622339242516 28.373277248753286 21.090455774654956 18-19 21.374167256900606 29.559504895424993 29.78425001131044 19.28207783636396 20-21 22.78736408936883 28.84981749320328 29.92256054697673 18.44025787045116 22-23 21.79930677451695 30.54402650939261 28.659231810054546 18.9974349060359 24-25 22.251819460269555 28.45708856702605 26.541042834065752 22.750049138638634 26-27 20.073827715560846 26.678193811915158 30.106987267294805 23.140991205229188 28-29 19.594914282835752 28.267037219032375 30.576786174240084 21.56126232389179 30-31 20.061409081973956 30.161608050289214 28.296484462182903 21.480498405553927 32-33 20.58665641693593 30.07227656936646 28.815467969021174 20.525599044676433 34-35 21.347972655725762 29.19597401525978 29.93352882337577 19.522524505638682 36-37 21.647157581626217 28.21527323934452 28.90047686339991 21.237092315629358 38-39 21.44480536761698 29.166210443555318 29.157542204439657 20.231441984388045 40-41 20.82762100070183 29.859303631484778 29.882228999716382 19.43084636809701 42-43 20.51035809467325 30.017716542916993 29.359581190049845 20.11234417235991 44-45 20.50225581518245 30.2042380507567 28.832291061938253 20.461215072122595 46-47 19.87643783587523 28.157593085081107 29.03638620590019 22.929582873143477 48-49 20.78092987412525 28.223580235508095 30.338043419167228 20.657446471199435 50-51 21.729524204914018 30.87341696596565 28.215311257633598 19.18174757148674 52-53 20.263736681525696 30.35777627814582 28.370607636083676 21.007879404244804 54-55 22.31385553038048 28.392346886490277 28.34973917272589 20.944058410403354 56-57 21.494562562000784 28.6673001250567 29.461404475349305 20.37673283759321 58-59 21.161601451578804 28.0794908219276 29.064922561677655 21.693985164815942 60-61 19.48244854168247 30.405178708124918 29.6986075967895 20.41376515340311 62-63 20.359934540073294 31.221126491726636 27.616819125127474 20.8021198430726 64-65 19.70687116466456 30.349091799410793 28.82944260203451 21.114594433890137 66-67 20.152050345339127 30.441015955135377 29.463128532611897 19.943805166913595 68-69 20.79167004078982 30.03503765521442 29.33529203558664 19.83800026840912 70-71 19.60143146462037 30.86621250018534 29.237699087523044 20.294656947671246 72-73 19.93679079257868 30.737919783691144 28.69745919972262 20.627830224007564 74-75 19.985397175165083 29.25551065595615 30.486371774005605 20.272720394873158 76-77 19.42120576524543 30.657587138869026 28.67016206816451 21.251045027721034 78-79 19.446126753736152 29.568078019612116 28.68793561830854 22.29785960834319 80-81 21.018248993131795 29.334513676052193 28.244565896142642 21.402671434673373 82-83 20.31992010076367 28.20337351486306 29.88562577913731 21.591080605235955 84-85 20.55080517033524 28.730230964907978 28.734317930983877 21.9846459337729 86-87 21.00174009709111 28.290017195672153 29.36559261198192 21.34265009525482 88-89 21.309289046588752 28.028793531416223 28.952181736546184 21.70973568544884 90-91 21.43161289169768 28.892131848947255 28.235023740520614 21.44123151883445 92-93 21.190196756051467 29.7069208113255 28.708180357242657 20.394702075380383 94-95 21.87469704175891 29.356677323193097 28.39781805435323 20.37080758069477 96-97 20.57089783611304 30.14413113572415 28.601367974077608 20.683603054085197 98-99 22.388077008325624 29.9976656770506 27.573353437404595 20.040903877219172 100 21.336434104990545 31.701284219786768 26.811866116313915 20.15041555890877 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 7.5 2 16.5 3 22.0 4 24.5 5 26.5 6 28.5 7 31.0 8 42.0 9 56.5 10 74.5 11 89.5 12 96.5 13 108.5 14 139.5 15 184.0 16 225.5 17 325.0 18 482.0 19 698.0 20 1153.0 21 1821.5 22 2706.5 23 4172.5 24 6507.0 25 9724.0 26 13870.5 27 19003.5 28 24657.5 29 30999.5 30 38569.0 31 46821.5 32 56103.0 33 66600.0 34 77880.0 35 90580.0 36 103473.0 37 113074.5 38 119725.5 39 124762.5 40 127642.5 41 128503.0 42 127426.5 43 125074.5 44 123952.0 45 121117.0 46 112006.5 47 102029.5 48 95325.0 49 91809.5 50 88630.0 51 81121.5 52 70218.0 53 58794.0 54 48909.5 55 40125.5 56 31656.0 57 23670.0 58 16612.5 59 11700.0 60 8908.0 61 7460.5 62 6190.5 63 4712.0 64 3471.0 65 2838.0 66 2760.0 67 2459.5 68 1781.0 69 1654.0 70 1936.5 71 1804.0 72 1180.0 73 570.5 74 262.0 75 164.5 76 122.5 77 100.0 78 86.0 79 62.0 80 51.0 81 39.5 82 27.5 83 24.5 84 25.5 85 20.5 86 16.0 87 18.5 88 18.0 89 12.5 90 10.5 91 13.0 92 11.0 93 13.0 94 16.5 95 13.5 96 11.5 97 17.5 98 28.0 99 73.0 100 90.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.332833773014848 2 0.00570274336172159 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 2.091005899297916E-4 30-31 0.009352499113223407 32-33 0.0 34-35 0.0 36-37 0.0 38-39 7.983840706410225E-4 40-41 0.0014827132740476132 42-43 1.140548672344318E-4 44-45 0.0 46-47 0.002205060766532348 48-49 0.0 50-51 0.0 52-53 0.0014446949849694693 54-55 0.019294281707158047 56-57 0.011633596457912045 58-59 0.01326838288827223 60-61 1.7108230085164768E-4 62-63 0.011671614746990187 64-65 7.603657815628786E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3306401177350376E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2630313.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.92057472760872 #Duplication Level Percentage of deduplicated Percentage of total 1 86.15876716785975 64.55064354038277 2 8.678436477671054 13.003868972883195 3 2.3757963226234073 5.33988077780055 4 1.0061975850536622 3.0153960544700937 5 0.5267865369420229 1.973357505323152 6 0.3159735469356062 1.4203751841082 7 0.22137507744014814 1.1609883622529351 8 0.1467491348946464 0.879562362166903 9 0.09690590022784057 0.6534221165609513 >10 0.4435542460608105 5.647426302908362 >50 0.02074244346062959 1.0521667989825738 >100 0.008405651203618079 1.1411557242277863 >500 3.0990962687187557E-4 0.1617562979324028 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 5.70274336172159E-5 0.0 16-17 0.0 0.0 0.0 7.603657815628786E-5 0.0 18-19 0.0 0.0 0.0 7.603657815628786E-5 0.0 20-21 0.0 0.0 0.0 7.603657815628786E-5 0.0 22-23 0.0 0.0 0.0 7.603657815628786E-5 0.0 24-25 0.0 0.0 0.0 7.603657815628786E-5 0.0 26-27 0.0 0.0 0.0 7.603657815628786E-5 0.0 28-29 0.0 0.0 0.0 7.603657815628786E-5 0.0 30-31 0.0 0.0 0.0 7.603657815628786E-5 0.0 32-33 0.0 0.0 0.0 7.603657815628786E-5 0.0 34-35 0.0 0.0 0.0 7.603657815628786E-5 0.0 36-37 0.0 0.0 0.0 7.603657815628786E-5 0.0 38-39 3.801828907814393E-5 0.0 0.0 7.603657815628786E-5 0.0 40-41 3.801828907814393E-5 0.0 0.0 7.603657815628786E-5 0.0 42-43 3.801828907814393E-5 0.0 0.0 7.603657815628786E-5 0.0 44-45 3.801828907814393E-5 0.0 0.0 7.603657815628786E-5 0.0 46-47 3.801828907814393E-5 0.0 0.0 7.603657815628786E-5 0.0 48-49 3.801828907814393E-5 0.0 0.0 7.603657815628786E-5 0.0 50-51 5.70274336172159E-5 0.0 0.0 7.603657815628786E-5 0.0 52-53 7.603657815628786E-5 0.0 0.0 7.603657815628786E-5 0.0 54-55 7.603657815628786E-5 0.0 0.0 7.603657815628786E-5 0.0 56-57 7.603657815628786E-5 0.0 0.0 7.603657815628786E-5 0.0 58-59 9.504572269535984E-5 0.0 0.0 7.603657815628786E-5 0.0 60-61 1.7108230085164768E-4 0.0 0.0 7.603657815628786E-5 0.0 62-63 2.4711887900793556E-4 0.0 0.0 7.603657815628786E-5 0.0 64-65 3.991920353205113E-4 0.0 0.0 7.603657815628786E-5 0.0 66-67 6.273017697893749E-4 0.0 0.0 7.603657815628786E-5 0.0 68-69 7.413566370238066E-4 0.0 0.0 7.603657815628786E-5 0.0 70-71 7.983840706410226E-4 0.0 0.0 7.603657815628786E-5 0.0 72-73 9.124389378754544E-4 0.0 0.0 7.603657815628786E-5 0.0 74-75 0.0010455029496489582 0.0 0.0 7.603657815628786E-5 0.0 76-77 0.0011595578168833898 0.0 0.0 7.603657815628786E-5 0.0 78-79 0.0013116309731959655 0.0 0.0 7.603657815628786E-5 0.0 80-81 0.001558749852203901 0.0 0.0 7.603657815628786E-5 0.0 82-83 0.0018628961648290527 0.0 0.0 7.603657815628786E-5 0.0 84-85 0.002014969321141628 0.0 0.0 7.603657815628786E-5 0.0 86-87 0.002014969321141628 3.801828907814393E-5 0.0 7.603657815628786E-5 0.0 88 0.002014969321141628 3.801828907814393E-5 0.0 7.603657815628786E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 10400 0.0 80.47316 8 CACCGGC 10000 0.0 79.843094 9 CTTCACC 11665 0.0 74.36186 6 CGGGCCC 775 0.0 72.6796 7 CCTTCAC 12620 0.0 72.156494 5 GGGCCCG 795 0.0 70.260765 8 GTGTGGC 4180 0.0 69.173134 7 CGTAGTG 2165 0.0 67.42726 4 TTCACCG 12875 0.0 66.75352 7 CGGGGCA 1040 0.0 65.44368 94 AACGGGC 875 0.0 63.836926 5 ACGGGCC 890 0.0 63.815823 6 GCTGCCA 140 0.0 63.706444 2 GCCTTCA 3700 0.0 63.17724 4 ACCTTCA 5095 0.0 61.356853 4 GCGTAGT 1505 0.0 61.12975 3 GGCCCGA 925 0.0 60.386276 9 AGCTGCT 95 0.0 59.294643 2 CAGCGTA 1000 0.0 59.293785 1 TGTGGCA 5100 0.0 58.443546 8 >>END_MODULE