FastQCFastQC Report
Mon 6 Mar 2017
1HL_3A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_3A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2211401
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA107620.48665981429871835No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG63060.2851585940315664No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA51660.23360756371187313No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG42800.1935424647090238No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG41510.1877090586465322No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG39960.18069992733113532No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT37750.1707062626814404No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC36390.16455631520470507No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA36220.16378757177011316No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG34050.1539747879285575No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA33380.15094503439222468No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT32570.14728219802740433No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT32260.14588037176432497No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA31800.14380024247072332No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA31340.14172011317712163No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA27910.12620958387917885No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA26160.1182960485230856No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC25440.11504019397657866No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA24840.11232698185448954No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC23230.10504652932688371No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG22820.10319250104345616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAG402.0008883E-1186.999853
GCTGCCT356.6393113E-1086.999843
CTGCAGC302.2699169E-886.999841
AGCTGCC1300.086.999842
GCAGCCA159.38833E-486.999843
GCAGCAC159.38833E-486.999843
AGCAGCA700.086.999842
CAGCTGC3150.085.618891
CAGCAGC1950.082.5383151
AGCTGCA950.082.42092
GCTGCCC850.081.88223
CAGCCCT129200.081.006821
CTGCTGC700.080.785571
CAGCGTA50850.079.6429141
AGCCCTT109150.079.6269762
GCCCTTC99350.078.111593
GCTGCTA455.638867E-1177.33323
CCCTTCA97350.076.945934
GCTGCAT401.9135769E-976.124863
CAGCACT55900.075.327231
Produced by FastQC (version 0.11.5)