##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_3A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2211401 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.803408337067768 12.0 12.0 12.0 2.0 32.0 2 14.68051113298764 12.0 12.0 12.0 12.0 22.0 3 13.742922247028016 12.0 12.0 12.0 12.0 22.0 4 13.756852330264842 12.0 12.0 12.0 12.0 22.0 5 22.450108324993973 22.0 22.0 22.0 22.0 27.0 6 26.864727383229003 27.0 22.0 32.0 22.0 32.0 7 35.00672198303248 37.0 37.0 37.0 27.0 41.0 8 37.61792637337145 41.0 37.0 41.0 27.0 41.0 9 38.54308106037756 41.0 41.0 41.0 32.0 41.0 10-11 38.89823397022973 41.0 41.0 41.0 37.0 41.0 12-13 39.07796369812621 41.0 41.0 41.0 37.0 41.0 14-15 39.032223011565975 41.0 41.0 41.0 37.0 41.0 16-17 38.942682715617835 41.0 41.0 41.0 37.0 41.0 18-19 38.88685317588262 41.0 41.0 41.0 37.0 41.0 20-21 38.90580518865642 41.0 41.0 41.0 37.0 41.0 22-23 38.88049611988056 41.0 41.0 41.0 37.0 41.0 24-25 38.89859618404803 41.0 41.0 41.0 37.0 41.0 26-27 38.865301001491815 41.0 41.0 41.0 37.0 41.0 28-29 38.81822993658771 41.0 41.0 41.0 37.0 41.0 30-31 38.69878755594304 41.0 41.0 41.0 32.0 41.0 32-33 38.73777777074352 41.0 41.0 41.0 32.0 41.0 34-35 38.7064847126324 41.0 41.0 41.0 32.0 41.0 36-37 38.640124970550346 41.0 41.0 41.0 32.0 41.0 38-39 38.564048311455046 41.0 41.0 41.0 32.0 41.0 40-41 38.59499543502061 41.0 41.0 41.0 32.0 41.0 42-43 38.34247429570666 41.0 41.0 41.0 32.0 41.0 44-45 38.447233902851636 41.0 41.0 41.0 32.0 41.0 46-47 38.45492585921775 41.0 41.0 41.0 32.0 41.0 48-49 38.41857107779187 41.0 41.0 41.0 32.0 41.0 50-51 38.30784082127122 41.0 41.0 41.0 32.0 41.0 52-53 38.27047446392581 41.0 41.0 41.0 32.0 41.0 54-55 38.14592129604716 41.0 39.0 41.0 32.0 41.0 56-57 37.938332758283096 41.0 37.0 41.0 29.5 41.0 58-59 37.94041085266761 41.0 37.0 41.0 29.5 41.0 60-61 37.95027473533746 41.0 37.0 41.0 32.0 41.0 62-63 37.93953832886935 41.0 37.0 41.0 32.0 41.0 64-65 37.92087188167139 41.0 37.0 41.0 32.0 41.0 66-67 37.789766080416896 41.0 37.0 41.0 27.0 41.0 68-69 37.80151632381463 41.0 37.0 41.0 27.0 41.0 70-71 37.77629272122062 41.0 37.0 41.0 27.0 41.0 72-73 37.63000921135516 41.0 37.0 41.0 27.0 41.0 74-75 37.551552386925756 41.0 37.0 41.0 27.0 41.0 76-77 36.49039771619891 39.0 34.5 41.0 24.5 41.0 78-79 37.00982024517489 41.0 37.0 41.0 27.0 41.0 80-81 37.65786779512173 41.0 37.0 41.0 27.0 41.0 82-83 37.84388177449499 41.0 37.0 41.0 32.0 41.0 84-85 37.75470979709243 41.0 37.0 41.0 27.0 41.0 86-87 37.60742126823675 41.0 37.0 41.0 27.0 41.0 88-89 37.526167574311486 41.0 37.0 41.0 27.0 41.0 90-91 37.38743922970099 41.0 37.0 41.0 27.0 41.0 92-93 37.28967247459868 41.0 37.0 41.0 27.0 41.0 94-95 37.141533353742716 41.0 37.0 41.0 27.0 41.0 96-97 37.08815859267496 41.0 37.0 41.0 27.0 41.0 98-99 36.95741274422866 41.0 37.0 41.0 27.0 41.0 100 36.83002540018748 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.3510039343519331 1101 2 -0.3242843730931906 1101 3 -0.1662277064482769 1101 4 0.021016968741694342 1101 5 -1.0463447549452134 1101 6 -0.5280187015098115 1101 7 -0.17716491023974612 1101 8 -0.04630353195319259 1101 9 -0.09612188395093568 1101 10-11 -0.053695681738282985 1101 12-13 0.0816451886447922 1101 14-15 -0.051545651803870385 1101 16-17 -0.025108869764849828 1101 18-19 0.1199107817638847 1101 20-21 -0.02136384963491622 1101 22-23 0.03786832496313508 1101 24-25 0.026379269123239624 1101 26-27 0.07629725059414483 1101 28-29 -0.005935168324789686 1101 30-31 0.08883886844056121 1101 32-33 -0.17991685573141325 1101 34-35 0.07529859147216911 1101 36-37 0.11008718627469705 1101 38-39 0.07896299272761098 1101 40-41 0.02343768291347459 1101 42-43 0.15717514472247274 1101 44-45 0.12894215207473536 1101 46-47 0.042415783209264646 1101 48-49 0.13341482778528047 1101 50-51 0.1377236774460826 1101 52-53 -0.037076290573473614 1101 54-55 0.014364622085267342 1101 56-57 0.17387229403207982 1101 58-59 -0.017824206496165118 1101 60-61 0.05727764626485765 1101 62-63 -0.06405417380869949 1101 64-65 -0.003650261529955401 1101 66-67 0.11573767130940382 1101 68-69 0.11291791555573383 1101 70-71 -0.10918175679680076 1101 72-73 -0.02354815988164205 1101 74-75 0.20028502763758382 1101 76-77 -0.5471281863001991 1101 78-79 -0.08913108642718726 1101 80-81 -0.11931089573049292 1101 82-83 -0.11803728583389272 1101 84-85 -0.19097665231509353 1101 86-87 -0.023198013446148025 1101 88-89 -0.12005174857027612 1101 90-91 0.0018852919517655664 1101 92-93 0.14121162802084797 1101 94-95 0.0987588406031108 1101 96-97 -0.013417735385516494 1101 98-99 -0.15177039338817622 1101 100 -0.3335730544411035 1102 1 1.7013719561935954 1102 2 0.8920253443573198 1102 3 -0.13558304571508195 1102 4 0.07965249897749871 1102 5 -0.8386082647179727 1102 6 -0.00943956368785237 1102 7 -0.10999566074022482 1102 8 -0.3840763123206514 1102 9 -0.31026750093270294 1102 10-11 -0.42073216370004474 1102 12-13 -0.2541213931317401 1102 14-15 -0.27854123263065134 1102 16-17 -0.3413573498780522 1102 18-19 -0.12036987030459301 1102 20-21 -0.010221435397077983 1102 22-23 -0.16176487482289303 1102 24-25 0.008191442045415442 1102 26-27 -0.06027903641662391 1102 28-29 -0.2666338977800038 1102 30-31 -0.22041397366328397 1102 32-33 -0.42899816571138416 1102 34-35 -0.10928545469391793 1102 36-37 -0.39489900413391155 1102 38-39 -0.30178885933795385 1102 40-41 -0.14964642797288974 1102 42-43 -0.2091640997293851 1102 44-45 -0.03951095015782613 1102 46-47 -0.4134267942068419 1102 48-49 -0.2924258730027489 1102 50-51 -0.17345907649782077 1102 52-53 -0.31338443290402296 1102 54-55 -0.3971202637809128 1102 56-57 -0.14312761707500954 1102 58-59 -0.16229142866553303 1102 60-61 -0.2981927505770017 1102 62-63 -0.25969602999661845 1102 64-65 -0.21437311163415984 1102 66-67 -0.2839575975604589 1102 68-69 -0.4962602513061327 1102 70-71 -0.6170056323924769 1102 72-73 -0.17526145373820867 1102 74-75 -0.2979012451139269 1102 76-77 -0.341590366006308 1102 78-79 -0.3803685588950856 1102 80-81 -0.26817259863791776 1102 82-83 -0.49606854702673076 1102 84-85 -0.5030045386964375 1102 86-87 -0.5466962821137855 1102 88-89 -0.48676709840026433 1102 90-91 -0.5536727761713962 1102 92-93 -0.4318012905620918 1102 94-95 -0.4803901579480936 1102 96-97 -0.39085702698700686 1102 98-99 -0.535338877057093 1102 100 -0.8211909480118607 1103 1 1.4804608447288476 1103 2 0.7003476707257636 1103 3 0.499548003474116 1103 4 1.1252239858991029 1103 5 2.8026018118268468 1103 6 -0.21755448170585723 1103 7 -0.5610202367731532 1103 8 -0.5046956729088166 1103 9 -0.44704932962719823 1103 10-11 -0.4972523183810509 1103 12-13 -0.5830495534568101 1103 14-15 -0.5117135725257356 1103 16-17 -0.47640299562883826 1103 18-19 -0.5127018615838992 1103 20-21 -0.3277368318865186 1103 22-23 -0.17443614584885125 1103 24-25 -0.3753336511822454 1103 26-27 -0.3514312932422641 1103 28-29 -0.44787962097737477 1103 30-31 -0.4196084760158527 1103 32-33 -0.5040786624305085 1103 34-35 -0.631394866577196 1103 36-37 -0.5644601047243114 1103 38-39 -0.2116953929662131 1103 40-41 -0.654226121050975 1103 42-43 -0.36090876997133137 1103 44-45 -0.7069839101561328 1103 46-47 -0.8386104646450434 1103 48-49 -0.6248412519871422 1103 50-51 -0.549025736268554 1103 52-53 -0.4656237534957981 1103 54-55 -0.5597803952279463 1103 56-57 -0.46759251944456537 1103 58-59 -0.7220764878379171 1103 60-61 -0.5859004904625351 1103 62-63 -0.40549534783001207 1103 64-65 -0.7890804324593432 1103 66-67 -0.6699599209583482 1103 68-69 -0.6934445901190713 1103 70-71 -0.6407769633599685 1103 72-73 -0.5126204187121957 1103 74-75 -0.5705344824347023 1103 76-77 -0.5988043042718232 1103 78-79 -0.6604775145860842 1103 80-81 -0.4756822662010478 1103 82-83 -0.5078568738089331 1103 84-85 -0.5924158384731228 1103 86-87 -0.5672076948776237 1103 88-89 -0.654377446538625 1103 90-91 -0.8538279345921183 1103 92-93 -0.47195125051264597 1103 94-95 -0.6523120535861437 1103 96-97 -0.45335404317121686 1103 98-99 -0.4546729424198972 1103 100 -0.7196515642958019 1104 1 1.5041203454279124 1104 2 -0.5368524763326654 1104 3 0.04998931293818387 1104 4 0.08070929213218037 1104 5 -1.1072336598469619 1104 6 -0.49453125023105926 1104 7 -0.23694585955755088 1104 8 -0.38361828206883786 1104 9 -0.1760080325074469 1104 10-11 -0.12452213034484316 1104 12-13 -0.11994592288475303 1104 14-15 -0.21091374105254346 1104 16-17 -0.04141945919564449 1104 18-19 -0.12303392381000577 1104 20-21 -0.16655113305036906 1104 22-23 0.02103281757707265 1104 24-25 -0.2846572360803208 1104 26-27 -0.2098883605000239 1104 28-29 -0.10722145400165317 1104 30-31 -0.22410690748206719 1104 32-33 -0.31041143311328057 1104 34-35 -0.20304559744148776 1104 36-37 -0.2253651044425382 1104 38-39 -0.1887193919357415 1104 40-41 -0.19963430211532085 1104 42-43 -0.23389215075873437 1104 44-45 -0.05102817796348802 1104 46-47 -0.2674184863066458 1104 48-49 -0.48605660402838424 1104 50-51 -0.28455309369836357 1104 52-53 -0.21771933610748562 1104 54-55 -0.16364752203363508 1104 56-57 -0.4256769760663559 1104 58-59 -0.22377718636546007 1104 60-61 -0.3002189632017078 1104 62-63 -0.19718372887236058 1104 64-65 -0.3287847323082502 1104 66-67 -0.6024888160611397 1104 68-69 -0.46691879183065055 1104 70-71 -0.26592985850570017 1104 72-73 -0.13026192890564658 1104 74-75 -0.3219976869666752 1104 76-77 -0.5227106913843542 1104 78-79 -0.33917322976503783 1104 80-81 -0.29874105468648793 1104 82-83 -0.3238080171345459 1104 84-85 -0.10518241541982576 1104 86-87 -0.27852904706377046 1104 88-89 -0.3682791642776806 1104 90-91 -0.4165020633111851 1104 92-93 -0.3233656144715269 1104 94-95 -0.37738632077628154 1104 96-97 -0.39279508696404264 1104 98-99 -0.20897856096429024 1104 100 -0.1695569692297738 1105 1 1.5706222869065165 1105 2 0.40264248073181896 1105 3 -0.011935381557343305 1105 4 -0.195377294863059 1105 5 -1.1388357250130703 1105 6 0.5159640402700703 1105 7 0.07681959060933963 1105 8 0.023127164771516107 1105 9 -0.2576120168273093 1105 10-11 -0.21889018434643503 1105 12-13 -0.15148258015757676 1105 14-15 -0.22168828800823093 1105 16-17 -0.2618657316376627 1105 18-19 -0.3324049818747241 1105 20-21 -0.18250635070721444 1105 22-23 -0.11721192178748652 1105 24-25 -0.10903873982475432 1105 26-27 -0.06707355465963616 1105 28-29 -0.1008240137088805 1105 30-31 -0.07310736781785465 1105 32-33 -0.12090740249873733 1105 34-35 -0.03083823379213868 1105 36-37 -0.34929739565362183 1105 38-39 -0.022279150597583453 1105 40-41 -0.04151091593433165 1105 42-43 0.038068647327229144 1105 44-45 -0.15728036312238913 1105 46-47 -0.26446481728938664 1105 48-49 -0.4147875890660302 1105 50-51 -0.13865628359301496 1105 52-53 -0.3165169797522225 1105 54-55 -0.22868777334616652 1105 56-57 -0.14148398805362206 1105 58-59 -0.16209310414003397 1105 60-61 -0.20906529590777012 1105 62-63 -0.2459952504792966 1105 64-65 -0.27000862496306155 1105 66-67 -0.33755786512436714 1105 68-69 -0.4572959982373277 1105 70-71 -0.566751204090977 1105 72-73 -0.31513753510365206 1105 74-75 -0.256829100406442 1105 76-77 -0.397882272868884 1105 78-79 -0.28033106175317357 1105 80-81 -0.566064681192529 1105 82-83 -0.2824340649337458 1105 84-85 -0.4211387036250258 1105 86-87 -0.4465581388236046 1105 88-89 -0.31492786488004043 1105 90-91 -0.19700218172427242 1105 92-93 -0.2779077814128357 1105 94-95 -0.3328328399666489 1105 96-97 -0.33723663019343064 1105 98-99 -0.4764224283280427 1105 100 -0.3896261997640025 1106 1 1.1626278244658685 1106 2 -0.4859958007581895 1106 3 -0.14856091836487195 1106 4 -0.17151342134547853 1106 5 -1.155939679986698 1106 6 -0.6859463658565055 1106 7 -0.42859547357424077 1106 8 -0.20702038681580603 1106 9 -0.2970699789484357 1106 10-11 -0.16512530507223033 1106 12-13 -0.1552169328581101 1106 14-15 -0.13547294798367915 1106 16-17 -0.17705578062277283 1106 18-19 -0.18359098138992636 1106 20-21 -0.1613552590743481 1106 22-23 -0.09407498255934854 1106 24-25 -0.3462201065597341 1106 26-27 -0.3012014862801138 1106 28-29 -0.207653410993764 1106 30-31 -0.04320065858249933 1106 32-33 -0.33017607393816917 1106 34-35 -0.2546739787896115 1106 36-37 -0.5065740623332502 1106 38-39 -0.3647961216874265 1106 40-41 -0.34918702056204864 1106 42-43 -0.2019995870183351 1106 44-45 -0.3148120012262936 1106 46-47 -0.4310668013222383 1106 48-49 -0.28399489625928 1106 50-51 -0.23677802434657025 1106 52-53 -0.08388374843338653 1106 54-55 -0.41892471400017683 1106 56-57 -0.35857809156398446 1106 58-59 -0.4478568323485277 1106 60-61 -0.2502577636863563 1106 62-63 -0.37926338268074744 1106 64-65 -0.6190186177170531 1106 66-67 -0.4947351097997199 1106 68-69 -0.41613619583694117 1106 70-71 -0.5450720221772372 1106 72-73 -0.4047636850351424 1106 74-75 -0.5655107024099237 1106 76-77 -0.5157753818999495 1106 78-79 -0.4370485282679226 1106 80-81 -0.4033037308374219 1106 82-83 -0.4135434304347001 1106 84-85 -0.593736728925137 1106 86-87 -0.6996391191479034 1106 88-89 -0.7459212164385818 1106 90-91 -0.6865064846101063 1106 92-93 -0.6563057260865008 1106 94-95 -0.7155789094272436 1106 96-97 -0.5746501544377622 1106 98-99 -0.8570655992548168 1106 100 -0.9279938900754772 1107 1 1.1627227007511447 1107 2 -0.5612168822687789 1107 3 -0.31980338920688567 1107 4 -0.1981117243776982 1107 5 -1.2150924084046473 1107 6 -0.1841586067421197 1107 7 -0.6304566300370951 1107 8 -0.3291053458938151 1107 9 -0.2795911317480062 1107 10-11 -0.4003338945385906 1107 12-13 -0.3377031659137941 1107 14-15 -0.49181272631577855 1107 16-17 -0.39103448353218084 1107 18-19 -0.29781781213986136 1107 20-21 -0.2676903594784932 1107 22-23 -0.3269091612140329 1107 24-25 -0.2020049903955794 1107 26-27 -0.22529097042984603 1107 28-29 -0.3464396270507848 1107 30-31 -0.3790457097959248 1107 32-33 -0.31567331132523435 1107 34-35 -0.2740304540301892 1107 36-37 -0.05422831206607981 1107 38-39 -0.2513140697769174 1107 40-41 -0.4281827287278688 1107 42-43 -0.2722652274398598 1107 44-45 -0.37489056406140975 1107 46-47 -0.3781539017053035 1107 48-49 -0.36513573088069506 1107 50-51 -0.3595534719452118 1107 52-53 -0.228473889469619 1107 54-55 -0.25222510417889765 1107 56-57 -0.450682012721785 1107 58-59 -0.45214405448948725 1107 60-61 -0.4234147589016928 1107 62-63 -0.38429143048257686 1107 64-65 -0.5603378988183607 1107 66-67 -0.6204928352989043 1107 68-69 -0.5342323977547636 1107 70-71 -0.39616263825375597 1107 72-73 -0.18937567397151867 1107 74-75 -0.40548287203291267 1107 76-77 -0.7619116110683422 1107 78-79 -0.9003739684214125 1107 80-81 -0.4517219982119727 1107 82-83 -0.4923882589524027 1107 84-85 -0.3965494154657492 1107 86-87 -0.4189216699495404 1107 88-89 -0.43044781671819976 1107 90-91 -0.23868251174472732 1107 92-93 -0.447855279838528 1107 94-95 -0.2530625531509756 1107 96-97 -0.38395791334130536 1107 98-99 -0.5960903237512198 1107 100 -0.7664878344647974 1108 1 1.4393492292922652 1108 2 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0.29502237516588536 2223 22-23 0.25560512827927084 2223 24-25 0.3458139433099703 2223 26-27 0.29627586050123256 2223 28-29 0.17219913679771537 2223 30-31 0.049299312633237946 2223 32-33 0.11550878054775637 2223 34-35 0.1894311950865557 2223 36-37 0.19814732514520728 2223 38-39 0.3615338074265182 2223 40-41 0.3533678710363972 2223 42-43 0.3982782771122402 2223 44-45 0.34440606454766964 2223 46-47 0.3070044224696886 2223 48-49 0.12472942337657145 2223 50-51 0.16022764423521352 2223 52-53 0.19988115701989528 2223 54-55 0.14456378572541695 2223 56-57 0.5105175835038551 2223 58-59 0.39480260371009734 2223 60-61 0.4071933318428691 2223 62-63 0.5429266011616889 2223 64-65 0.3652551935886379 2223 66-67 0.47260165461832315 2223 68-69 0.46071664747302066 2223 70-71 0.5093145083206565 2223 72-73 0.3940127916937328 2223 74-75 0.47179255712055124 2223 76-77 0.4859390090825144 2223 78-79 0.5472970518118316 2223 80-81 0.4079063981544735 2223 82-83 0.2468828070009721 2223 84-85 0.1959972660625624 2223 86-87 0.3641740671170126 2223 88-89 0.4320680297590158 2223 90-91 0.4956134958933518 2223 92-93 0.5045580322177585 2223 94-95 0.6107325977553941 2223 96-97 0.5429903799517604 2223 98-99 0.4906314674364083 2223 100 0.656325208478016 2224 1 1.2057028008345103 2224 2 -0.8443112144293536 2224 3 -0.08150309465919925 2224 4 -0.4565709964982183 2224 5 -1.1119470662768691 2224 6 0.531688590226203 2224 7 0.3232432532321923 2224 8 0.058806472215771066 2224 9 0.08914838861114305 2224 10-11 0.26395915396737735 2224 12-13 0.14781880227484834 2224 14-15 0.09095136702075735 2224 16-17 0.04442421400446506 2224 18-19 -0.05222523323931938 2224 20-21 0.02197349234887014 2224 22-23 0.03490980424374612 2224 24-25 0.03774747611235796 2224 26-27 0.151493618356902 2224 28-29 -0.010262840188190125 2224 30-31 0.03174407604958418 2224 32-33 0.22131555748544685 2224 34-35 0.14094684492485499 2224 36-37 0.06582011751111594 2224 38-39 0.07689838165234875 2224 40-41 0.09703202486915785 2224 42-43 0.2613096065529277 2224 44-45 0.07528529845949805 2224 46-47 0.26985548069495735 2224 48-49 0.07299954644863504 2224 50-51 0.1655395434500022 2224 52-53 0.005376254253000923 2224 54-55 -0.04373406260319257 2224 56-57 0.1084532449352622 2224 58-59 0.021269880689580134 2224 60-61 0.12384917181918098 2224 62-63 0.06140477279288348 2224 64-65 0.11122060820375168 2224 66-67 0.10777753438922844 2224 68-69 0.13255519808053862 2224 70-71 0.2697988495531405 2224 72-73 0.19907444926126772 2224 74-75 0.1706996938813674 2224 76-77 0.23156089640903588 2224 78-79 0.10995295677308548 2224 80-81 0.15367313742073918 2224 82-83 -0.045461643966774545 2224 84-85 -0.11623679482932658 2224 86-87 0.2506633496856949 2224 88-89 0.11705933492212495 2224 90-91 0.22700839278927987 2224 92-93 0.10311700345150143 2224 94-95 0.23644547888775946 2224 96-97 0.05765118209914988 2224 98-99 0.11590979274955515 2224 100 0.25982601198462163 2225 1 1.6828303105708944 2225 2 1.4091865059229285 2225 3 0.8642501330407679 2225 4 0.9447354351538344 2225 5 3.229741000809412 2225 6 -0.03422246649994065 2225 7 0.38567310879239614 2225 8 0.5396129165366688 2225 9 0.19951220319681084 2225 10-11 0.25162938231967047 2225 12-13 0.2150143809953562 2225 14-15 0.27516525992972873 2225 16-17 0.367788280476681 2225 18-19 0.23686711075124123 2225 20-21 0.16087823800059908 2225 22-23 0.3192618869777917 2225 24-25 0.4690297723637258 2225 26-27 0.26049143285613496 2225 28-29 0.2552548860166084 2225 30-31 0.29840162611265697 2225 32-33 0.4274567451081239 2225 34-35 0.39545670700330504 2225 36-37 0.48891535596121827 2225 38-39 0.3226215270445394 2225 40-41 0.5121342128043338 2225 42-43 0.5814010110613452 2225 44-45 0.37453172547126456 2225 46-47 0.39601436992995787 2225 48-49 0.2851559535827377 2225 50-51 0.28201036460509954 2225 52-53 0.498905701379428 2225 54-55 0.43224915191619573 2225 56-57 0.38103161915917383 2225 58-59 0.4834024405772155 2225 60-61 0.45402796158135317 2225 62-63 0.48523505293344726 2225 64-65 0.2526483180085961 2225 66-67 0.4800087076091657 2225 68-69 0.6773256708174245 2225 70-71 0.3992081681627795 2225 72-73 0.6463040947669825 2225 74-75 0.4292572534336543 2225 76-77 0.6804966136721049 2225 78-79 0.5773361338324321 2225 80-81 0.4251188916420716 2225 82-83 0.376179285062328 2225 84-85 0.3084514893662629 2225 86-87 0.23899198560829404 2225 88-89 0.41638016037468617 2225 90-91 0.46018639608914214 2225 92-93 0.420683580740679 2225 94-95 0.5217218908310599 2225 96-97 0.6476817413518532 2225 98-99 0.7269592156710303 2225 100 0.6036951084869742 2226 1 1.2697713788673841 2226 2 1.3973723490241277 2226 3 0.890303349546075 2226 4 0.9081674387184275 2226 5 3.357040022348933 2226 6 -0.3264831248681084 2226 7 0.029536933055851478 2226 8 0.1842318990305003 2226 9 0.36598879251899774 2226 10-11 -0.09131573477879584 2226 12-13 0.16839057187628015 2226 14-15 -0.003607762480342558 2226 16-17 0.07797763666049917 2226 18-19 0.14448604430916845 2226 20-21 0.20278900467164362 2226 22-23 0.1301761384001523 2226 24-25 0.1905753484294337 2226 26-27 0.1154049837224207 2226 28-29 0.13737499532395958 2226 30-31 0.15268969888806794 2226 32-33 0.08668940441380357 2226 34-35 0.06601545197771941 2226 36-37 0.2211346248588555 2226 38-39 0.12947706053601138 2226 40-41 0.20009224640526924 2226 42-43 0.3329209859237494 2226 44-45 0.19550668316747277 2226 46-47 0.14883011595778584 2226 48-49 0.27931442348587154 2226 50-51 0.20161833652104377 2226 52-53 0.25893202374339097 2226 54-55 0.11697071951341798 2226 56-57 0.27762580917985247 2226 58-59 0.3052229591432436 2226 60-61 0.25219956175958913 2226 62-63 0.24453544982935682 2226 64-65 0.35593436380744947 2226 66-67 0.4782544843120746 2226 68-69 0.5038211133396331 2226 70-71 0.3522266118465751 2226 72-73 0.3224386994035271 2226 74-75 0.527691304579399 2226 76-77 0.5310895107547537 2226 78-79 0.41317240975880054 2226 80-81 0.41971482275062044 2226 82-83 0.20018466286036585 2226 84-85 0.2073116691928263 2226 86-87 0.5123761350121754 2226 88-89 0.41990985545250936 2226 90-91 0.4490355782156854 2226 92-93 0.3490527531830736 2226 94-95 0.5632715620590645 2226 96-97 0.3702162089678751 2226 98-99 0.5232478138634917 2226 100 0.7370080947874982 2227 1 1.4267228374468424 2227 2 1.8231451299970303 2227 3 0.7103274969229698 2227 4 -0.12983222373759418 2227 5 3.2062747062909267 2227 6 0.09787326964290699 2227 7 0.24205202516640867 2227 8 0.12904605510563982 2227 9 0.23585390475236778 2227 10-11 0.058149387840551015 2227 12-13 0.07367832811760167 2227 14-15 0.2556954688541211 2227 16-17 0.1370075871973242 2227 18-19 0.14663983453856844 2227 20-21 0.04564930041210147 2227 22-23 0.1805669104670926 2227 24-25 0.010776057271783657 2227 26-27 0.1824366739089882 2227 28-29 -0.04674745524532398 2227 30-31 0.008965420388321377 2227 32-33 -0.12813851306967905 2227 34-35 -0.12636124710182628 2227 36-37 0.05911870836204258 2227 38-39 0.01774091606718997 2227 40-41 -0.08277609846081191 2227 42-43 0.1540233266614166 2227 44-45 0.03311362618745051 2227 46-47 0.14604018515429829 2227 48-49 0.08598740828340823 2227 50-51 0.018662360045262005 2227 52-53 0.025328222478897544 2227 54-55 0.11834036911680812 2227 56-57 0.2303105820517004 2227 58-59 0.10473936248232718 2227 60-61 0.300299578636789 2227 62-63 0.2089278057565025 2227 64-65 0.2675212099782698 2227 66-67 0.16650990124379916 2227 68-69 0.09972255049802925 2227 70-71 0.3551262444429355 2227 72-73 0.20737320288932182 2227 74-75 0.11857307330983957 2227 76-77 0.41883861171935166 2227 78-79 0.2764839758330311 2227 80-81 0.24174670767409623 2227 82-83 0.2536295588059403 2227 84-85 0.20688519535897143 2227 86-87 0.16127716971429606 2227 88-89 0.18537326438703872 2227 90-91 0.3025836847237997 2227 92-93 0.1595633533622305 2227 94-95 0.24428081855353412 2227 96-97 0.19712300788253856 2227 98-99 0.479987009210582 2227 100 0.4361992874861613 2228 1 1.4805465661737145 2228 2 1.79915812082929 2228 3 -0.23015249898542933 2228 4 0.09722604321378014 2228 5 3.1661003018106264 2228 6 0.05939304358197006 2228 7 0.2881311209226496 2228 8 0.2948453876632726 2228 9 0.3132273528210092 2228 10-11 0.20762259424901686 2228 12-13 0.23581312078851369 2228 14-15 0.28589084278807064 2228 16-17 0.24174489364543916 2228 18-19 0.1349071695000177 2228 20-21 0.1324462163680593 2228 22-23 0.22792910663377342 2228 24-25 0.256867939280248 2228 26-27 0.02754848968466206 2228 28-29 0.21599340578089965 2228 30-31 0.28151909068202485 2228 32-33 0.1852173666884127 2228 34-35 0.20129797370396574 2228 36-37 0.2557226797719778 2228 38-39 0.15958264783719045 2228 40-41 0.09530570798090565 2228 42-43 0.4692651786221802 2228 44-45 0.34647462044642197 2228 46-47 0.2050052658994801 2228 48-49 0.21397012127040682 2228 50-51 0.32915156205525875 2228 52-53 0.3405950076315065 2228 54-55 0.272837933335758 2228 56-57 0.3499572979028187 2228 58-59 0.36522025342838305 2228 60-61 0.35245853732440224 2228 62-63 0.2066039044152248 2228 64-65 0.19631329273089193 2228 66-67 0.3426002489019879 2228 68-69 0.42742396593615695 2228 70-71 0.4872011974534587 2228 72-73 0.4950542881864095 2228 74-75 0.3975019689612438 2228 76-77 0.4319617816870007 2228 78-79 0.39752886836458856 2228 80-81 0.278055188795193 2228 82-83 0.3294405268531051 2228 84-85 0.3694374591649279 2228 86-87 0.3091872567886753 2228 88-89 0.4367490651260866 2228 90-91 0.37295474176326593 2228 92-93 0.3915775716747021 2228 94-95 0.5961060560581757 2228 96-97 0.3654367569395589 2228 98-99 0.4343573502199334 2228 100 0.8422037115294287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 24.0 13 23.0 14 117.0 15 380.0 16 1528.0 17 3644.0 18 5935.0 19 7650.0 20 8892.0 21 10123.0 22 11759.0 23 13627.0 24 15333.0 25 17558.0 26 19722.0 27 22182.0 28 25096.0 29 28524.0 30 32668.0 31 37621.0 32 44213.0 33 52299.0 34 64401.0 35 83011.0 36 116029.0 37 190681.0 38 472298.0 39 924876.0 40 1187.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.287430795194588 19.504582363156615 19.360458828114957 35.84752801353383 2 18.250848604291274 52.38314675947574 12.717532576184452 16.648472060048533 3 41.249642195151395 14.743730332038377 25.13379527277052 18.872832200039703 4 16.851624829689413 16.857548676155975 21.502070406950164 44.78875608720445 5 17.423976926844116 35.07916474669226 26.3154895923444 21.18136873411923 6 17.75896818351805 28.26158620711486 30.025988050109408 23.953457559257682 7 20.34257920657538 26.302963596380756 35.874542880282675 17.47991431676118 8 21.568001461516932 27.975704089850733 33.209264172350466 17.24703027628187 9 21.153920071484094 28.219169657606198 27.197012210811156 23.42989806009855 10-11 19.755869695274626 30.174061601672424 28.437651063737423 21.63241763931553 12-13 24.204881882571275 26.73818543086487 27.247884938100324 21.80904774846353 14-15 21.692583118122855 27.420829600782493 28.643629084006022 22.242958197088633 16-17 20.89580315826935 29.63684560149878 28.30420624753267 21.1631449926992 18-19 21.36331221700632 29.549796712581756 29.5284301671203 19.558460903291625 20-21 22.743478003311026 28.966297835625472 29.778249173261656 18.51197498780185 22-23 21.768598277743383 30.457117456309373 28.56044652236297 19.213837743584268 24-25 22.271989566794986 28.41045111221348 26.55079291363258 22.766766407358954 26-27 20.212842446937486 26.643675208612095 29.95695941170326 23.18652293274716 28-29 19.723699043996252 28.111262900805055 30.47771246449636 21.68732559070233 30-31 20.26924324941576 30.086299392071492 28.06212470844418 21.582332650068572 32-33 20.78268482287925 30.035800833950965 28.64720147996677 20.53431286320301 34-35 21.470122334212565 29.020991669986586 29.93914265210154 19.56974334369931 36-37 21.803101291895953 28.057620485836804 28.76662803354073 21.372650188726514 38-39 21.465892942537078 29.387769867944602 28.81658871769131 20.329748471827013 40-41 20.98481098921324 29.72467049250272 29.810318813892845 19.480199704391197 42-43 20.58652024951631 29.843151290913873 29.27407701506535 20.296251444504463 44-45 20.58439423695657 30.14410321782436 28.731989358782055 20.539513186437013 46-47 19.977086379645723 28.020210292497993 28.967166414520374 23.035536913335914 48-49 20.892976895642175 28.168138659609905 30.09223564609042 20.8466487986575 50-51 21.729889784801582 30.802961561471665 28.147947839401354 19.3192008143254 52-53 20.304956578835505 30.35253354942922 28.275580139678496 21.066929732056774 54-55 22.296930119044415 28.36952728912476 28.194197916174335 21.139344675656492 56-57 21.442059973258086 28.706472308006454 29.275455981779498 20.57601173695597 58-59 21.20133959563388 27.922744859493964 28.96552191993632 21.910393624935836 60-61 19.642935874654714 30.269302799974046 29.53793204336357 20.54982928200767 62-63 20.40716784734835 31.118959709689793 27.450623023654636 21.02324941930722 64-65 19.895414622146433 30.16282905075023 28.746734533207256 21.195021793896082 66-67 20.27721340453405 30.34124068859515 29.297581035732552 20.083964871138253 68-69 20.933516806766388 29.942963759173484 29.078398716469785 20.045120717590343 70-71 19.771674155885794 30.827561351378606 29.105282126579485 20.295482366156115 72-73 20.059048539817066 30.764388729135966 28.52076579507742 20.65579693596955 74-75 20.02174187313834 29.28277141956615 30.249873270383798 20.445613436911714 76-77 19.564316919455134 30.53724765431507 28.714715241604754 21.18372018462504 78-79 19.516225234591104 29.359351831712114 28.548372728419675 22.576050205277106 80-81 20.99451636104154 29.46468442000338 28.29653117385326 21.244268045101816 82-83 20.242597339876394 28.234092324277686 29.68283454696819 21.84047578887773 84-85 20.836564693603737 29.18079986397763 28.2512533909499 21.731382051468728 86-87 21.244518746260855 29.541476195407345 28.972922595223572 20.241082463108228 88-89 20.592149501605544 29.19967929832717 29.74060335506767 20.467567844999618 90-91 20.071529315578676 30.951080333236714 29.102297593245186 19.875092757939424 92-93 19.9123315943151 30.064628712748164 30.87694181200063 19.146097880936114 94-95 19.8805191821836 31.077764729237256 29.60107642168924 19.440639666889904 96-97 19.569879004305417 30.41558270074039 30.23795774714762 19.77658054780657 98-99 21.47109456855631 30.964691614049194 28.941291063900216 18.62292275349428 100 19.97367279837533 32.22658396193182 28.967383120474306 18.832360119218542 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3.0 1 12.0 2 19.0 3 15.0 4 20.0 5 25.5 6 26.0 7 36.0 8 45.0 9 53.5 10 74.5 11 80.5 12 90.0 13 111.5 14 138.5 15 167.5 16 201.0 17 275.5 18 400.5 19 637.0 20 1047.0 21 1583.0 22 2389.0 23 3740.5 24 5677.5 25 8477.5 26 12152.5 27 16584.0 28 21663.0 29 27657.5 30 34683.5 31 42407.5 32 51293.5 33 60815.0 34 71589.0 35 82845.5 36 93087.5 37 100824.0 38 105270.5 39 107340.5 40 108171.5 41 107778.0 42 105485.5 43 102662.5 44 100061.5 45 96648.5 46 89289.5 47 79559.5 48 72806.0 49 69414.0 50 66293.5 51 61374.5 52 53911.0 53 45446.0 54 38120.5 55 31548.5 56 24945.0 57 18932.5 58 14046.5 59 10770.5 60 9015.0 61 7809.0 62 6653.0 63 5499.0 64 4478.0 65 3992.0 66 4155.5 67 3847.0 68 2903.5 69 2568.0 70 2779.5 71 2464.0 72 1545.5 73 840.5 74 500.5 75 322.0 76 241.5 77 183.0 78 134.0 79 96.0 80 69.5 81 50.0 82 29.5 83 20.5 84 21.5 85 16.5 86 11.0 87 13.0 88 14.5 89 14.5 90 11.0 91 10.0 92 10.5 93 9.0 94 7.5 95 8.5 96 14.5 97 15.0 98 32.5 99 76.0 100 73.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.362796706703126 2 0.005562084850282694 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.617616162785492E-4 30-31 0.009202311114085596 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.36573737644145E-4 40-41 0.0014696565661316062 42-43 1.1305050508704662E-4 44-45 0.0 46-47 0.0023740606068279792 48-49 0.0 50-51 0.0 52-53 0.00167314747528829 54-55 0.018969874753606425 56-57 0.012005963640244352 58-59 0.012932977781958134 60-61 2.487111111915026E-4 62-63 0.011259830306669844 64-65 9.04404040696373E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.0349090915668393E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2211401.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.70799696821278 #Duplication Level Percentage of deduplicated Percentage of total 1 87.28909073267576 66.95771307280143 2 8.088125917262792 12.408478766798348 3 2.0990544289030844 4.830427823052342 4 0.9257350617313836 2.8404512923463696 5 0.48320655194292184 1.8532903360729096 6 0.29498708138470775 1.357672088671206 7 0.18040344090030933 0.9686870618345256 8 0.1268064104529567 0.7781652598860277 9 0.09520916133739245 0.6572973653293303 >10 0.3911854717602424 5.140706306460303 >50 0.018384465964676446 0.963437121267215 >100 0.007391328947646017 1.0313420176550856 >500 4.1994673621870555E-4 0.21233148782489045 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 4.522020203481865E-5 0.0 3 0.0 0.0 0.0 4.522020203481865E-5 0.0 4 0.0 0.0 0.0 4.522020203481865E-5 0.0 5 0.0 0.0 0.0 4.522020203481865E-5 0.0 6 0.0 0.0 0.0 4.522020203481865E-5 0.0 7 0.0 0.0 0.0 4.522020203481865E-5 0.0 8 0.0 0.0 0.0 4.522020203481865E-5 0.0 9 0.0 0.0 0.0 4.522020203481865E-5 0.0 10-11 0.0 0.0 0.0 4.522020203481865E-5 0.0 12-13 0.0 0.0 0.0 4.522020203481865E-5 0.0 14-15 0.0 0.0 0.0 4.522020203481865E-5 0.0 16-17 0.0 0.0 0.0 4.522020203481865E-5 0.0 18-19 0.0 0.0 0.0 4.522020203481865E-5 0.0 20-21 0.0 0.0 0.0 4.522020203481865E-5 0.0 22-23 0.0 2.2610101017409324E-5 0.0 4.522020203481865E-5 0.0 24-25 0.0 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 26-27 0.0 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 28-29 0.0 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 30-31 0.0 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 32-33 0.0 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 34-35 0.0 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 36-37 0.0 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 38-39 0.0 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 40-41 0.0 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 42-43 2.2610101017409324E-5 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 44-45 9.04404040696373E-5 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 46-47 9.04404040696373E-5 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 48-49 1.3566060610445594E-4 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 50-51 1.5827070712186525E-4 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 52-53 1.808808081392746E-4 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 54-55 1.808808081392746E-4 4.522020203481865E-5 0.0 4.522020203481865E-5 0.0 56-57 1.808808081392746E-4 4.522020203481865E-5 1.5827070712186528E-4 4.522020203481865E-5 0.0 58-59 1.808808081392746E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 60-61 1.808808081392746E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 62-63 2.2610101017409324E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 64-65 3.617616162785492E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 66-67 4.0698181831336786E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 68-69 4.295919193307772E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 70-71 4.5220202034818653E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 72-73 4.7481212136559587E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 74-75 5.878626264526424E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 76-77 6.783030305222798E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 78-79 7.68743434591917E-4 4.522020203481865E-5 3.1654141424373056E-4 4.522020203481865E-5 0.0 80-81 0.0010174545457834196 4.522020203481865E-5 3.1654141424373056E-4 9.04404040696373E-5 0.0 82-83 0.001243555555957513 4.522020203481865E-5 3.1654141424373056E-4 9.04404040696373E-5 0.0 84-85 0.0012887757579923315 4.522020203481865E-5 3.1654141424373056E-4 9.04404040696373E-5 0.0 86-87 0.001311385859009741 4.522020203481865E-5 3.1654141424373056E-4 9.04404040696373E-5 0.0 88 0.001311385859009741 4.522020203481865E-5 3.1654141424373056E-4 9.04404040696373E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 8470 0.0 80.575516 8 CACCGGC 8325 0.0 76.56629 9 CTTCACC 9580 0.0 73.6893 6 CCTTCAC 10360 0.0 72.400085 5 CGTAGTG 1915 0.0 70.10022 4 GTGTGGC 3315 0.0 67.68095 7 TTCACCG 10485 0.0 67.015526 7 CAGCGTA 870 0.0 63.942814 1 AACGGGC 755 0.0 63.412563 5 CGGGCCC 775 0.0 62.987404 7 GCGTAGT 1305 0.0 61.864285 3 GGGCCCG 785 0.0 61.587082 8 GGCCCGA 800 0.0 60.43233 9 ACCTTCA 4485 0.0 60.28124 4 TGTGGCA 3820 0.0 60.208084 8 ACGGGCC 815 0.0 59.32008 6 AGCAGCG 40 8.098688E-6 58.673492 2 GCCTTCA 3010 0.0 57.385666 4 CGGGGCA 1020 0.0 57.061554 94 AGCGTAG 1200 0.0 56.71771 2 >>END_MODULE