FastQCFastQC Report
Tue 7 Mar 2017
1HL_3A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_3A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2211401
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG84700.080.5755168
CACCGGC83250.076.566299
CTTCACC95800.073.68936
CCTTCAC103600.072.4000855
CGTAGTG19150.070.100224
GTGTGGC33150.067.680957
TTCACCG104850.067.0155267
CAGCGTA8700.063.9428141
AACGGGC7550.063.4125635
CGGGCCC7750.062.9874047
GCGTAGT13050.061.8642853
GGGCCCG7850.061.5870828
GGCCCGA8000.060.432339
ACCTTCA44850.060.281244
TGTGGCA38200.060.2080848
ACGGGCC8150.059.320086
AGCAGCG408.098688E-658.6734922
GCCTTCA30100.057.3856664
CGGGGCA10200.057.06155494
AGCGTAG12000.056.717712
Produced by FastQC (version 0.11.5)