##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_4A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2571031 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.77901355526246 12.0 12.0 12.0 2.0 27.0 2 14.66661312135093 12.0 12.0 12.0 12.0 22.0 3 13.738417389755316 12.0 12.0 12.0 12.0 22.0 4 13.729494899128015 12.0 12.0 12.0 12.0 22.0 5 22.45031156761626 22.0 22.0 22.0 22.0 27.0 6 26.85029585407566 27.0 22.0 32.0 22.0 32.0 7 35.022244772622344 37.0 37.0 37.0 27.0 41.0 8 37.652218506894705 41.0 37.0 41.0 32.0 41.0 9 38.60396626878478 41.0 41.0 41.0 32.0 41.0 10-11 38.95737079794059 41.0 41.0 41.0 37.0 41.0 12-13 39.13354409184487 41.0 41.0 41.0 37.0 41.0 14-15 39.092523194002716 41.0 41.0 41.0 37.0 41.0 16-17 39.00300638148664 41.0 41.0 41.0 37.0 41.0 18-19 38.939715040386524 41.0 41.0 41.0 37.0 41.0 20-21 38.952375136666966 41.0 41.0 41.0 37.0 41.0 22-23 38.932242162774386 41.0 41.0 41.0 37.0 41.0 24-25 38.95108596512449 41.0 41.0 41.0 37.0 41.0 26-27 38.9252338847723 41.0 41.0 41.0 37.0 41.0 28-29 38.876587057876776 41.0 41.0 41.0 37.0 41.0 30-31 38.744145247568 41.0 41.0 41.0 32.0 41.0 32-33 38.79159138882417 41.0 41.0 41.0 37.0 41.0 34-35 38.76547423971162 41.0 41.0 41.0 32.0 41.0 36-37 38.689730501110255 41.0 41.0 41.0 32.0 41.0 38-39 38.60324924125769 41.0 41.0 41.0 32.0 41.0 40-41 38.6489785615187 41.0 41.0 41.0 32.0 41.0 42-43 38.38572113677354 41.0 41.0 41.0 32.0 41.0 44-45 38.49326612553485 41.0 41.0 41.0 32.0 41.0 46-47 38.51126396375617 41.0 41.0 41.0 32.0 41.0 48-49 38.462154676470256 41.0 41.0 41.0 32.0 41.0 50-51 38.3521083565309 41.0 41.0 41.0 32.0 41.0 52-53 38.33063156375789 41.0 41.0 41.0 32.0 41.0 54-55 38.1936520796521 41.0 41.0 41.0 32.0 41.0 56-57 37.998449843661945 41.0 37.0 41.0 32.0 41.0 58-59 37.99458991354052 41.0 37.0 41.0 29.5 41.0 60-61 38.00488247710743 41.0 37.0 41.0 32.0 41.0 62-63 37.99779738167295 41.0 37.0 41.0 32.0 41.0 64-65 37.97768716129833 41.0 37.0 41.0 32.0 41.0 66-67 37.848017585163305 41.0 37.0 41.0 29.5 41.0 68-69 37.86139957083364 41.0 37.0 41.0 29.5 41.0 70-71 37.847719844684875 41.0 37.0 41.0 29.5 41.0 72-73 37.709851806532086 41.0 37.0 41.0 27.0 41.0 74-75 37.62723631103631 41.0 37.0 41.0 27.0 41.0 76-77 36.580255352813715 39.0 34.5 41.0 24.5 41.0 78-79 37.102095229501316 41.0 37.0 41.0 27.0 41.0 80-81 37.750519149710755 41.0 37.0 41.0 29.5 41.0 82-83 37.938712135326256 41.0 37.0 41.0 32.0 41.0 84-85 37.84527024372713 41.0 37.0 41.0 29.5 41.0 86-87 37.69041174532707 41.0 37.0 41.0 27.0 41.0 88-89 37.62098609468342 41.0 37.0 41.0 27.0 41.0 90-91 37.46054753910008 41.0 37.0 41.0 27.0 41.0 92-93 37.35671059586602 41.0 37.0 41.0 27.0 41.0 94-95 37.192448671369576 41.0 37.0 41.0 27.0 41.0 96-97 37.14041682111184 41.0 37.0 41.0 27.0 41.0 98-99 37.02508254470678 41.0 37.0 41.0 27.0 41.0 100 36.89470099738198 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.5260774111075417 1101 2 -0.6242205288884737 1101 3 -0.1227443038231879 1101 4 0.06859513142083884 1101 5 -1.208617182354132 1101 6 -0.94295655219452 1101 7 -0.29100649890164476 1101 8 0.006751910043483633 1101 9 0.026671878323355713 1101 10-11 -0.08631969144761342 1101 12-13 -0.04239422085324662 1101 14-15 0.015444153889163204 1101 16-17 -0.008837761573417424 1101 18-19 -0.008905599623240334 1101 20-21 0.051753778732177125 1101 22-23 0.03937051689650417 1101 24-25 0.007354827744904924 1101 26-27 0.006507686584349415 1101 28-29 -0.040869725628546405 1101 30-31 -0.1205957729398861 1101 32-33 -0.029948811492829464 1101 34-35 0.08590536719800212 1101 36-37 0.06442039195157179 1101 38-39 -0.07114930496797456 1101 40-41 0.0116756952436603 1101 42-43 0.1943225139026552 1101 44-45 0.13216267652034475 1101 46-47 0.02945814699927496 1101 48-49 0.10886156566967742 1101 50-51 -0.07293727010048201 1101 52-53 0.0672477330391743 1101 54-55 0.08041113885705897 1101 56-57 0.20018702307378078 1101 58-59 0.14956353741143857 1101 60-61 0.09836662900386983 1101 62-63 -0.06094485858830012 1101 64-65 0.0981028634770027 1101 66-67 -0.03086746934781104 1101 68-69 0.10465339281984853 1101 70-71 0.004006217427587444 1101 72-73 0.07102741309548577 1101 74-75 0.08695392734976792 1101 76-77 -0.13180182559545273 1101 78-79 -0.07942576712559912 1101 80-81 -0.010228308668679631 1101 82-83 -0.08821291299898348 1101 84-85 -0.012689524025923049 1101 86-87 -0.05703377992952596 1101 88-89 -0.21031093607594897 1101 90-91 -0.08314400985262438 1101 92-93 -0.061359952223135394 1101 94-95 -0.09493684647585354 1101 96-97 -0.23031590619491027 1101 98-99 -0.3847164503670868 1101 100 -0.23172893113456894 1102 1 1.753898526333007 1102 2 0.8093825585946668 1102 3 0.027208670435703297 1102 4 0.004491319951137385 1102 5 -0.7475855921209842 1102 6 -0.3053636215083628 1102 7 -0.26119065284846954 1102 8 -0.35597661311931716 1102 9 -0.2591904478578613 1102 10-11 -0.3244734017118134 1102 12-13 -0.3821704622660391 1102 14-15 -0.2577456412388557 1102 16-17 -0.4907109122599138 1102 18-19 -0.422849828811259 1102 20-21 -0.3792482034075775 1102 22-23 -0.3254594750337674 1102 24-25 -0.267734215925131 1102 26-27 -0.2642047200080313 1102 28-29 -0.31175735795402915 1102 30-31 -0.4204288458669865 1102 32-33 -0.3684873125067867 1102 34-35 -0.29131719523749666 1102 36-37 -0.4922345612039223 1102 38-39 -0.36023961919148917 1102 40-41 -0.3342435578387679 1102 42-43 -0.2159686832219947 1102 44-45 -0.2113340354932518 1102 46-47 -0.5340909896539046 1102 48-49 -0.5697634703388701 1102 50-51 -0.45864344792837386 1102 52-53 -0.46588938944332625 1102 54-55 -0.31992223835425193 1102 56-57 -0.1988038789238189 1102 58-59 -0.16927480278020823 1102 60-61 -0.1630284807006035 1102 62-63 -0.5189946543649526 1102 64-65 -0.3770428400109225 1102 66-67 -0.432530826089085 1102 68-69 -0.5708686119838333 1102 70-71 -0.6144838651965472 1102 72-73 -0.43826317546075444 1102 74-75 -0.5792962801167363 1102 76-77 -0.385045170928457 1102 78-79 -0.4414382208391032 1102 80-81 -0.5236503899726515 1102 82-83 -0.4359585390327325 1102 84-85 -0.6305160953819993 1102 86-87 -0.48285797042861134 1102 88-89 -0.5336285496483626 1102 90-91 -0.6863709486390377 1102 92-93 -0.5197845231251677 1102 94-95 -0.2277362039496964 1102 96-97 -0.2586621262982689 1102 98-99 -0.34591181990547426 1102 100 -0.7219677990065421 1103 1 1.788045647592968 1103 2 0.878464177671221 1103 3 0.4455558349864823 1103 4 1.0675926502501412 1103 5 2.8324875256717377 1103 6 -0.19439039231128774 1103 7 -0.5761569634580965 1103 8 -0.4223755916149017 1103 9 -0.4716339761785946 1103 10-11 -0.3637277471765117 1103 12-13 -0.4558169788446449 1103 14-15 -0.4779997861359959 1103 16-17 -0.36306406603564767 1103 18-19 -0.3568339144714727 1103 20-21 -0.24655976424011072 1103 22-23 -0.24259340450945643 1103 24-25 -0.4001704562963937 1103 26-27 -0.3180366523472671 1103 28-29 -0.3437614844823642 1103 30-31 -0.21741417663464802 1103 32-33 -0.4402330047726224 1103 34-35 -0.550033642372064 1103 36-37 -0.4867001876994905 1103 38-39 -0.2382644657374584 1103 40-41 -0.4262697070808841 1103 42-43 -0.4787035309395975 1103 44-45 -0.6558444421687781 1103 46-47 -0.8370825241147841 1103 48-49 -0.5777596615413074 1103 50-51 -0.48037553734575056 1103 52-53 -0.36269272005608144 1103 54-55 -0.39925732715006745 1103 56-57 -0.5221754590982073 1103 58-59 -0.7589940280731682 1103 60-61 -0.5266756128825563 1103 62-63 -0.4938403067158603 1103 64-65 -0.5821929578691751 1103 66-67 -0.6478216192443753 1103 68-69 -0.6554261023873735 1103 70-71 -0.7498336966999446 1103 72-73 -0.7585358877495878 1103 74-75 -0.6771807889546153 1103 76-77 -0.7784805554124716 1103 78-79 -0.7994045254912763 1103 80-81 -0.6124152314746496 1103 82-83 -0.5346096726365417 1103 84-85 -0.5690531636404899 1103 86-87 -0.3899734516616462 1103 88-89 -0.48190704463715406 1103 90-91 -0.7868401972653416 1103 92-93 -0.618975203287448 1103 94-95 -0.7084213506320935 1103 96-97 -0.621331839208672 1103 98-99 -0.5355278819900704 1103 100 -0.40352035068247716 1104 1 1.8072316292277009 1104 2 -0.5227544141329208 1104 3 0.08574968659329407 1104 4 -0.04047878063532906 1104 5 -1.0905147744862838 1104 6 -0.5059808129990309 1104 7 -0.09495298611651037 1104 8 -0.3126303368157082 1104 9 -0.30825736339826193 1104 10-11 -0.2562493539034847 1104 12-13 -0.07127993214195527 1104 14-15 -0.12810489507853617 1104 16-17 0.0849804996760426 1104 18-19 9.819433002959954E-4 1104 20-21 -0.05184424831239198 1104 22-23 -0.0901764387742432 1104 24-25 -0.07298437627201793 1104 26-27 -0.16083129035134647 1104 28-29 -0.18357413619140317 1104 30-31 -0.0026247724799617345 1104 32-33 -0.15382853452289424 1104 34-35 -0.07148468572230371 1104 36-37 -0.1765217184744543 1104 38-39 -0.25507529723798683 1104 40-41 -0.059201022706481865 1104 42-43 -0.053257687842290125 1104 44-45 -0.13867634825225394 1104 46-47 -0.25751949414554076 1104 48-49 -0.3681525122134133 1104 50-51 -0.1184486120582946 1104 52-53 -0.17626143262752691 1104 54-55 -0.09199365128877446 1104 56-57 -0.05120370532671359 1104 58-59 -0.0770868647067573 1104 60-61 -0.1138488422588253 1104 62-63 -0.12954183091552096 1104 64-65 -0.34725557328203394 1104 66-67 -0.4481338264001806 1104 68-69 -0.4838774493056732 1104 70-71 -0.3494967121507173 1104 72-73 -0.42693577208175526 1104 74-75 -0.24233603462277387 1104 76-77 -0.28135815268923636 1104 78-79 -0.27462023279886694 1104 80-81 -0.49019818870754506 1104 82-83 -0.4802259937995572 1104 84-85 -0.3927059153710104 1104 86-87 -0.39979501843282605 1104 88-89 -0.4563707310573406 1104 90-91 -0.40159372519421055 1104 92-93 -0.3747354973045205 1104 94-95 -0.334972937405162 1104 96-97 -0.27992335538116464 1104 98-99 -0.4761584276566637 1104 100 -0.35279088742201026 1105 1 1.6069963234168263 1105 2 0.5200166931797039 1105 3 -0.004633864189884207 1105 4 0.05798907342421522 1105 5 -1.1295869807787504 1105 6 0.4243115741183203 1105 7 0.17738170750630644 1105 8 -0.13214869956804876 1105 9 -0.10046418749791997 1105 10-11 -0.11052819822372584 1105 12-13 -0.2758477657451479 1105 14-15 -0.34608550661938864 1105 16-17 -0.13479611004800063 1105 18-19 -0.08902224042778073 1105 20-21 -0.10266267566981213 1105 22-23 -0.08644459956347106 1105 24-25 -0.05584120378106405 1105 26-27 -0.22735632279835727 1105 28-29 -0.1709634320884632 1105 30-31 -0.17262975365896693 1105 32-33 -0.19835923465014105 1105 34-35 -0.15717916724629077 1105 36-37 -0.14541795392819523 1105 38-39 -0.19309371257585894 1105 40-41 -0.17298022356818876 1105 42-43 -0.05254518277154574 1105 44-45 -0.021956980480602795 1105 46-47 -0.2440131024029597 1105 48-49 -0.1772496937444572 1105 50-51 -0.023833948438117147 1105 52-53 -0.05263660208584753 1105 54-55 -0.19222109816293909 1105 56-57 -0.22091022026966556 1105 58-59 -0.34196568105842573 1105 60-61 -0.252676381359926 1105 62-63 -0.4662868673613332 1105 64-65 -0.3009326310810465 1105 66-67 -0.29856150280152605 1105 68-69 -0.4445483829126857 1105 70-71 -0.41230839700273947 1105 72-73 -0.3785333898993528 1105 74-75 -0.3658372024838812 1105 76-77 -0.5167936938059228 1105 78-79 -0.22088388625674327 1105 80-81 -0.6337852963530182 1105 82-83 -0.30808812031225585 1105 84-85 -0.44438593645145374 1105 86-87 -0.5680612098649647 1105 88-89 -0.3895119827316975 1105 90-91 -0.45137084343769374 1105 92-93 -0.4576571032551229 1105 94-95 -0.2214531970274507 1105 96-97 -0.32647137521804837 1105 98-99 -0.6313987204251745 1105 100 -0.5629238305822497 1106 1 1.5748659079450427 1106 2 -0.7098795322434288 1106 3 -0.19567155441598238 1106 4 -0.22716549099663474 1106 5 -1.156813370927722 1106 6 -0.5425086390080729 1106 7 -0.24009114031989753 1106 8 -0.20693561243766112 1106 9 -0.07422473124812967 1106 10-11 -0.07724929660281532 1106 12-13 -0.1291125480633113 1106 14-15 -0.010262334223504865 1106 16-17 -0.047106659630181014 1106 18-19 -0.0024060064089539424 1106 20-21 0.07361170536098172 1106 22-23 -0.06123229769001881 1106 24-25 -0.14178910753767582 1106 26-27 -0.20321560935220617 1106 28-29 -0.1507668152434647 1106 30-31 -0.09819285470371142 1106 32-33 -0.24463777754269245 1106 34-35 -0.1534128068489764 1106 36-37 -0.35688276263864793 1106 38-39 -0.14262563240840365 1106 40-41 -0.1794873221951221 1106 42-43 -0.07912143763606849 1106 44-45 -0.22410538599724106 1106 46-47 -0.3478680244604675 1106 48-49 -0.3561132201111832 1106 50-51 -0.30095025897161065 1106 52-53 -0.15803953369896817 1106 54-55 -0.18805109654837793 1106 56-57 -0.3707013323486237 1106 58-59 -0.10851272053444205 1106 60-61 -0.03513157718481352 1106 62-63 -0.3161402828746773 1106 64-65 -0.5426197766740017 1106 66-67 -0.43199638326339596 1106 68-69 -0.4288442976039022 1106 70-71 -0.5005042423806643 1106 72-73 -0.31242212971711325 1106 74-75 -0.3195060856818941 1106 76-77 -0.3935619822963048 1106 78-79 -0.31181884189259534 1106 80-81 -0.352391346547023 1106 82-83 -0.25407431779364487 1106 84-85 -0.40068878208852254 1106 86-87 -0.4742726113743032 1106 88-89 -0.5256608835347905 1106 90-91 -0.5758071582383835 1106 92-93 -0.37046159860209116 1106 94-95 -0.20612134265851978 1106 96-97 -0.14687660227126287 1106 98-99 -0.6587545822053968 1106 100 -0.5424879976611763 1107 1 1.3326192206871212 1107 2 -0.8070154407239869 1107 3 -0.20117793181274024 1107 4 -0.2153919994455613 1107 5 -1.1976053356869052 1107 6 -0.26649279862750674 1107 7 -0.2636055619506408 1107 8 -0.16683209021677925 1107 9 -0.27580330362918204 1107 10-11 -0.049406622003559164 1107 12-13 -0.2738257086347815 1107 14-15 -0.2709242779262695 1107 16-17 -0.1528297840118995 1107 18-19 -0.19052554654794562 1107 20-21 -0.2360034064179004 1107 22-23 -0.008633698453508032 1107 24-25 -0.06379808442522972 1107 26-27 -0.15938922556720314 1107 28-29 -0.05439881479691877 1107 30-31 -0.2053097263525956 1107 32-33 -0.05793882850294807 1107 34-35 -0.19436544696409186 1107 36-37 -0.00343651852684701 1107 38-39 0.016419421038925464 1107 40-41 -0.2189901046503806 1107 42-43 -0.16396393702360257 1107 44-45 0.018043229209901313 1107 46-47 -0.22833961147557602 1107 48-49 -0.15435032654377778 1107 50-51 -0.2684976165306807 1107 52-53 -0.16095601430008344 1107 54-55 -0.2585989161702287 1107 56-57 -0.25427110460438485 1107 58-59 -0.26705287735669003 1107 60-61 -0.2552998424646731 1107 62-63 -0.3369541374706486 1107 64-65 -0.1810368472579711 1107 66-67 -0.20032674730064315 1107 68-69 -0.25784753669503147 1107 70-71 -0.2706854741036011 1107 72-73 -0.14280690552362785 1107 74-75 -0.3312516674003305 1107 76-77 -0.3194793048440303 1107 78-79 -0.5405156930432611 1107 80-81 -0.31483527491798924 1107 82-83 -0.1954217076040763 1107 84-85 -0.16000601454952346 1107 86-87 -0.11176158311404549 1107 88-89 -0.11939247079798321 1107 90-91 -0.030457944210276366 1107 92-93 -0.08301561575733274 1107 94-95 -0.0981959580455225 1107 96-97 -0.133629801048194 1107 98-99 -0.3248609202182777 1107 100 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16-17 0.42508802655775924 2223 18-19 0.1947235383296615 2223 20-21 0.37828994750569933 2223 22-23 0.41976426539915934 2223 24-25 0.3635523502579048 2223 26-27 0.38283852551950304 2223 28-29 0.2894630017210389 2223 30-31 0.2470435680880314 2223 32-33 0.40312042567229867 2223 34-35 0.3438717985174904 2223 36-37 0.3918894938272999 2223 38-39 0.31633219843540417 2223 40-41 0.4828226956241579 2223 42-43 0.5722122812330852 2223 44-45 0.5036168965629031 2223 46-47 0.37870940476784654 2223 48-49 0.3732280308115179 2223 50-51 0.38249614919798347 2223 52-53 0.4879684309069603 2223 54-55 0.4660095830290416 2223 56-57 0.6000537066516216 2223 58-59 0.569334391002954 2223 60-61 0.5263611046870125 2223 62-63 0.5622271139592314 2223 64-65 0.44691499442585325 2223 66-67 0.47058322753348847 2223 68-69 0.6227032525980363 2223 70-71 0.5701041049400928 2223 72-73 0.6581602644603564 2223 74-75 0.6817207645374452 2223 76-77 0.7594319854785496 2223 78-79 0.8453885622758932 2223 80-81 0.6749259353313803 2223 82-83 0.5534432546471209 2223 84-85 0.5803760490514946 2223 86-87 0.5812689019008204 2223 88-89 0.6706335529952057 2223 90-91 0.6465210079745773 2223 92-93 0.6246522520074862 2223 94-95 0.9100184093761143 2223 96-97 0.6809260786132185 2223 98-99 0.8718658013557317 2223 100 0.7995432119339654 2224 1 1.3027778509733796 2224 2 -0.9315526368444829 2224 3 -0.07395136532221791 2224 4 -0.21014579242518394 2224 5 -1.1031939247077744 2224 6 0.563012728705786 2224 7 0.0785075015014769 2224 8 0.13975206888868996 2224 9 0.2631409076075144 2224 10-11 0.28306311387318317 2224 12-13 0.18275846981868682 2224 14-15 0.12076529675850622 2224 16-17 0.06537479619866815 2224 18-19 0.10900598061198963 2224 20-21 0.1340370270079987 2224 22-23 0.09680335712319987 2224 24-25 0.22323967278691725 2224 26-27 0.19338554682306608 2224 28-29 0.16770061219306598 2224 30-31 0.19488415983789054 2224 32-33 0.11212611882409362 2224 34-35 0.2593321083788993 2224 36-37 0.1183358500693501 2224 38-39 0.11347154219757272 2224 40-41 0.21887334730934072 2224 42-43 0.37933618355305754 2224 44-45 0.41033162413175006 2224 46-47 0.2053398380199809 2224 48-49 0.044870878232238454 2224 50-51 0.17890284580884952 2224 52-53 0.21291860996198153 2224 54-55 0.17293542186544641 2224 56-57 0.3912047746455869 2224 58-59 0.19178108517321846 2224 60-61 0.34555580296873956 2224 62-63 0.3398111149354648 2224 64-65 0.22810403240992372 2224 66-67 0.05550609072418666 2224 68-69 0.15707993930572428 2224 70-71 0.38018056119950927 2224 72-73 0.09981357826895021 2224 74-75 0.16533631486762346 2224 76-77 0.4044903954324113 2224 78-79 0.20210324291025472 2224 80-81 0.2791766968432299 2224 82-83 0.2753073981164249 2224 84-85 0.02500331892002805 2224 86-87 0.22634693046514087 2224 88-89 0.20357179191984187 2224 90-91 0.18190209244254874 2224 92-93 0.12607586849649977 2224 94-95 0.3148313520268786 2224 96-97 0.21127043685467584 2224 98-99 0.09159311205726084 2224 100 0.41196639193236706 2225 1 1.397679080588155 2225 2 1.6875703698931233 2225 3 0.8772117402509778 2225 4 0.9158580784564432 2225 5 3.1455522195611962 2225 6 -0.0181505533047428 2225 7 0.18367815092126705 2225 8 0.35975196916324137 2225 9 0.15194492332117449 2225 10-11 0.10407396170059258 2225 12-13 0.13714826897930266 2225 14-15 0.14016304710582972 2225 16-17 0.2296788007891024 2225 18-19 0.16645455104768558 2225 20-21 0.18001784213313243 2225 22-23 0.1351476960547089 2225 24-25 0.1955801485079931 2225 26-27 0.318066713058478 2225 28-29 0.2040005794370856 2225 30-31 0.20916283032889993 2225 32-33 0.19601391925658618 2225 34-35 0.09970839167281298 2225 36-37 0.32260051297048875 2225 38-39 0.1457745326569153 2225 40-41 0.19907295546504145 2225 42-43 0.3653984973829125 2225 44-45 0.31599269262088114 2225 46-47 0.35826210049890506 2225 48-49 0.3424588073105639 2225 50-51 0.2537204249505791 2225 52-53 0.30911598273340957 2225 54-55 0.3141214374425587 2225 56-57 0.3647121340001007 2225 58-59 0.48120562331015293 2225 60-61 0.4475460736005914 2225 62-63 0.33520734715460776 2225 64-65 0.32529424872976875 2225 66-67 0.39950077765915637 2225 68-69 0.25416573157453826 2225 70-71 0.3240632296070274 2225 72-73 0.5117483363465212 2225 74-75 0.39616687826270436 2225 76-77 0.4121588029474168 2225 78-79 0.340438357237808 2225 80-81 0.40720374775461465 2225 82-83 0.28256357514904096 2225 84-85 0.17456751871152676 2225 86-87 0.19133447629090483 2225 88-89 0.3310622432081729 2225 90-91 0.28485509720865565 2225 92-93 0.21447461533117718 2225 94-95 0.527399213276567 2225 96-97 0.24128645429588857 2225 98-99 0.33509293063366385 2225 100 0.45474343114518234 2226 1 1.4351353810964174 2226 2 1.2858130904403282 2226 3 0.8829576777640256 2226 4 0.8453809046795548 2226 5 3.210311924549295 2226 6 -0.4663763824088889 2226 7 0.14754014213771427 2226 8 0.10357425599892167 2226 9 0.30864038768402224 2226 10-11 0.13498833916550979 2226 12-13 0.1754125118820511 2226 14-15 0.12701201482988722 2226 16-17 0.1251892829733805 2226 18-19 0.1799758665304978 2226 20-21 0.221577529660415 2226 22-23 0.30763068124718274 2226 24-25 0.14826123806300728 2226 26-27 0.1347078211371482 2226 28-29 0.15712179546915905 2226 30-31 0.31652683523311254 2226 32-33 0.31969164191048094 2226 34-35 0.19958512209677082 2226 36-37 0.17446967450962347 2226 38-39 0.2036077970326744 2226 40-41 0.1308841926398756 2226 42-43 0.3318007528650426 2226 44-45 0.21605285652481854 2226 46-47 0.27774514642464965 2226 48-49 0.19079044517320654 2226 50-51 0.2908010331943629 2226 52-53 0.25991111420197655 2226 54-55 0.2606058346837443 2226 56-57 0.20562188055254182 2226 58-59 0.395293656197218 2226 60-61 0.34265827833247187 2226 62-63 0.2115022945254239 2226 64-65 0.2047226536840796 2226 66-67 0.33887928642941745 2226 68-69 0.3212494334196947 2226 70-71 0.379859556762014 2226 72-73 0.45886255666408005 2226 74-75 0.44633304071663815 2226 76-77 0.45346435966280296 2226 78-79 0.2921848185753859 2226 80-81 0.2756677082476102 2226 82-83 0.262424420024864 2226 84-85 0.28260461915297697 2226 86-87 0.42090475038777697 2226 88-89 0.31848789238998876 2226 90-91 0.4481164932389774 2226 92-93 0.17985000392926054 2226 94-95 0.3820075405682033 2226 96-97 0.3745506402611838 2226 98-99 0.4843667883735492 2226 100 0.5519331884777756 2227 1 1.464362766225765 2227 2 1.8745002247185987 2227 3 0.5357064064823298 2227 4 -0.06466233929175758 2227 5 3.214242677282755 2227 6 0.24059472788647795 2227 7 0.3364245728852282 2227 8 0.3913719436463623 2227 9 0.36505427773487753 2227 10-11 0.27378744867547766 2227 12-13 0.2924986706810415 2227 14-15 0.3614061961017043 2227 16-17 0.4052483960308919 2227 18-19 0.3600831654564658 2227 20-21 0.19928758163096205 2227 22-23 0.2477711221858172 2227 24-25 0.298723699089841 2227 26-27 0.257353946206031 2227 28-29 0.22965822180406548 2227 30-31 0.2831867672606876 2227 32-33 0.19207051563221 2227 34-35 0.319579506735721 2227 36-37 0.3173312890587212 2227 38-39 0.3318818038868301 2227 40-41 0.32672228053356633 2227 42-43 0.4502742473175019 2227 44-45 0.24230305690404208 2227 46-47 0.32297166269069066 2227 48-49 0.3871294574198174 2227 50-51 0.3560068523019595 2227 52-53 0.3617616222878084 2227 54-55 0.46322238425306494 2227 56-57 0.47039782100695504 2227 58-59 0.5196459214547389 2227 60-61 0.45833044748384566 2227 62-63 0.46747340576933283 2227 64-65 0.39608438822357783 2227 66-67 0.3491101517546653 2227 68-69 0.4117876449082303 2227 70-71 0.4763854315299767 2227 72-73 0.46510091925515695 2227 74-75 0.46071576361659083 2227 76-77 0.6321977922135034 2227 78-79 0.4673911973220086 2227 80-81 0.46905969933892067 2227 82-83 0.3718440933284697 2227 84-85 0.3232235988836152 2227 86-87 0.19621556346740476 2227 88-89 0.30232324089807605 2227 90-91 0.23577398656836834 2227 92-93 0.3644227479911635 2227 94-95 0.45768781025730476 2227 96-97 0.2687855994881616 2227 98-99 0.5362974955770525 2227 100 0.2942983368153165 2228 1 1.2218577669232094 2228 2 1.4717276471943492 2228 3 -0.14877872888709476 2228 4 -0.08318371285142767 2228 5 3.261396976193513 2228 6 0.16842951550247776 2228 7 0.27575074426940205 2228 8 0.38586635758579035 2228 9 0.28578547197098203 2228 10-11 0.2898621147156746 2228 12-13 0.22534942048701367 2228 14-15 0.34994747274935634 2228 16-17 0.3359806425535936 2228 18-19 0.2782694826811536 2228 20-21 0.2687448972911497 2228 22-23 0.24885616332006322 2228 24-25 0.22618378608514433 2228 26-27 0.2819118493583943 2228 28-29 0.31633651821418596 2228 30-31 0.2173037435048002 2228 32-33 0.26896273494228495 2228 34-35 0.2948871970306399 2228 36-37 0.32071997191403767 2228 38-39 0.29587167181639273 2228 40-41 0.2661446448427114 2228 42-43 0.39537651206791224 2228 44-45 0.4419660616510086 2228 46-47 0.4892905476961005 2228 48-49 0.2851330495008284 2228 50-51 0.2302112605733484 2228 52-53 0.3883379767867865 2228 54-55 0.37698589761775736 2228 56-57 0.7195512385571021 2228 58-59 0.5541279790270437 2228 60-61 0.4877059972753557 2228 62-63 0.5579589466173545 2228 64-65 0.4469322319113971 2228 66-67 0.5897498365882328 2228 68-69 0.4811655214014081 2228 70-71 0.6124242520578846 2228 72-73 0.6894930355013784 2228 74-75 0.6980251213810504 2228 76-77 0.7523389906267539 2228 78-79 0.7221709597612502 2228 80-81 0.6533340950223945 2228 82-83 0.5828761229370656 2228 84-85 0.43192885152556926 2228 86-87 0.5222437877140038 2228 88-89 0.542804047397631 2228 90-91 0.5578134045842305 2228 92-93 0.6918077442667467 2228 94-95 0.8313969473239382 2228 96-97 0.6652412572792912 2228 98-99 0.8100776196339226 2228 100 0.8872302086938717 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 14.0 13 32.0 14 119.0 15 426.0 16 1576.0 17 4007.0 18 6416.0 19 8237.0 20 9768.0 21 11332.0 22 13185.0 23 14809.0 24 17124.0 25 19427.0 26 21956.0 27 25061.0 28 28260.0 29 32297.0 30 37002.0 31 42997.0 32 50249.0 33 60925.0 34 74438.0 35 96788.0 36 135725.0 37 225805.0 38 556720.0 39 1075042.0 40 1294.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.10321510533452 19.295327193024796 19.222310470264553 35.37914723137613 2 18.510661222571912 52.99536069461608 11.682632147465108 16.811345935346903 3 41.25481178562219 14.498658320339194 24.884569653185824 19.361960240852795 4 17.92331558818233 15.721747423504423 21.5452478013684 44.809689186944844 5 17.673065785671195 35.91271361566624 26.039864941340653 20.374355657321907 6 17.685862208584805 27.994800529437413 30.92658159314298 23.3927556688348 7 20.297577119840252 26.757981525699226 36.13262539424846 16.81181596021207 8 20.8867571025009 27.796125367605445 34.04058527493446 17.2765322549592 9 21.027828913770392 28.114635723956656 27.739766653922104 23.11776870835085 10-11 19.68323213527958 30.105043463108768 28.732092300715163 21.479632100896488 12-13 24.21619964909019 26.789992030434483 27.260931509577286 21.73287681089804 14-15 21.68596955851563 27.38249752725658 28.74704739071602 22.184485523511775 16-17 20.948483312725518 29.487625781252735 28.333964079001774 21.229926827019977 18-19 21.331306390315792 29.51279078315275 29.788575089137392 19.367327737394067 20-21 22.840992582353152 29.027460190094946 29.71195213126563 18.419595096286276 22-23 21.84716948181488 30.344616614891066 28.66935482302625 19.1388590802678 24-25 21.97871204197849 28.427097922973317 26.665100498593752 22.92908953645444 26-27 20.27007842379186 26.627450232999912 29.965799712255514 23.13667163095272 28-29 19.931291927628017 28.041022589261665 30.489814849928386 21.537870633181935 30-31 20.147309663428345 30.036117513201273 28.24562631891161 21.57094650445877 32-33 20.79461507854242 29.89347464110701 28.768245112563797 20.543665167786777 34-35 21.404720518733537 29.197197544487018 29.944854807273813 19.453227129505635 36-37 21.89625484873578 28.05948275224997 28.832071647521946 21.2121907514923 38-39 21.554114184008764 29.360274258189783 28.874064180274818 20.211547377526635 40-41 20.97895542796555 29.665979578727388 29.83443572732819 19.52062926597888 42-43 20.685862242182257 29.922289793590224 29.190753187491453 20.201094776736063 44-45 20.553816737332223 30.130500176777332 28.729194630480926 20.586488455409523 46-47 20.002726584627975 28.055272265616793 28.932991362833345 23.009009786921887 48-49 20.87930872867733 28.343104381082917 30.148586306427266 20.629000583812488 50-51 21.929685017411302 30.545372654005337 28.19656783601598 19.32837449256738 52-53 20.296355964770076 30.260704411831057 28.337286405735362 21.105653217663505 54-55 22.25775963366317 28.258699906497895 28.144501689437256 21.339038770401675 56-57 21.55199274298231 28.716099913138294 29.29083424195168 20.441073101927724 58-59 21.183207554472673 28.006677518379608 29.0106976053943 21.79941732175342 60-61 19.60775962743271 30.405552181415548 29.488311181666617 20.498377009485125 62-63 20.476605497717276 31.04196623399306 27.328650730932928 21.15277753735673 64-65 19.887931921355147 30.196829943215285 28.92358363478556 20.991654500644003 66-67 20.253139693764872 30.122468379416663 29.491106875024066 20.1332850517944 68-69 21.01215426807378 29.884878867660486 29.20849651365542 19.89447035061032 70-71 19.66613782564271 30.77699179823192 29.326114698733697 20.230755677391677 72-73 20.094370701870183 30.709256325575225 28.43034564732981 20.766027325224783 74-75 19.99936990257994 29.315943681737018 30.257433690997892 20.427252724685154 76-77 19.641828511597097 30.424779008887874 28.569492160926877 21.363900318588147 78-79 19.58731730578122 29.416311977568533 28.53355327104185 22.462817445608398 80-81 20.983443581105917 29.456205633001908 28.358395302733108 21.201955483159065 82-83 20.337950028607203 27.952268953583214 29.914186176673873 21.795594841135717 84-85 20.886659865244724 29.116743438721667 28.274221508803276 21.722375187230337 86-87 21.37212659046118 28.818011140277967 29.27455950550577 20.535302763755084 88-89 20.70115062789986 28.47785577070055 28.912486858384828 21.908506743014765 90-91 20.04876642872062 29.788789011100995 28.720482172326978 21.44196238785141 92-93 19.928328363213048 29.319560907667004 29.425471727100916 21.326639002019036 94-95 20.081515936602866 30.421511837080146 29.05950958973268 20.43746263658431 96-97 19.62409243606942 29.779318102348824 29.47496549049778 21.121623971083974 98-99 21.492234049297736 30.208795615455436 28.247792422572893 20.051177912673943 100 20.176147234319615 31.321598222658537 28.37208108342529 20.13017345959656 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 3.0 2 11.0 3 19.0 4 24.0 5 30.5 6 33.5 7 36.5 8 41.5 9 49.5 10 68.5 11 81.0 12 84.0 13 105.5 14 135.5 15 167.0 16 205.5 17 290.0 18 420.0 19 673.0 20 1093.5 21 1709.5 22 2660.0 23 4062.5 24 6211.5 25 9254.0 26 13396.0 27 18296.0 28 23567.0 29 29831.0 30 37326.0 31 45802.0 32 55230.0 33 65794.5 34 76920.0 35 89267.0 36 102156.0 37 111776.5 38 118607.5 39 123873.0 40 126662.0 41 126595.5 42 124722.0 43 122355.0 44 120988.5 45 117812.5 46 108521.0 47 98388.0 48 92314.5 49 88940.0 50 85622.5 51 78829.5 52 68330.5 53 57166.5 54 47040.5 55 37750.0 56 28972.5 57 21091.0 58 14792.5 59 10767.0 60 8412.5 61 6933.0 62 5700.5 63 4557.0 64 4149.5 65 4547.5 66 4027.0 67 2524.0 68 1812.0 69 2150.0 70 2871.5 71 2771.5 72 1633.0 73 631.5 74 265.5 75 172.0 76 122.0 77 83.5 78 65.5 79 57.0 80 41.0 81 27.0 82 24.5 83 24.0 84 19.0 85 12.0 86 16.0 87 22.0 88 17.0 89 15.0 90 15.5 91 11.5 92 12.5 93 13.0 94 9.0 95 7.5 96 14.5 97 24.5 98 40.0 99 90.5 100 90.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.361239907258994 2 0.006689923225352008 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.3060667102030274E-4 30-31 0.00995709503308206 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.945827568784663E-4 40-41 0.0013807690377906762 42-43 1.7502706112839558E-4 44-45 0.0 46-47 0.001905850221175863 48-49 0.0 50-51 0.0 52-53 0.0013613215865541878 54-55 0.019466898687724887 56-57 0.010812782887487549 58-59 0.01227134173022418 60-61 2.1392196360137236E-4 62-63 0.012037972315386317 64-65 1.7502706112839558E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.555796098919072E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2571031.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.1277904994524 #Duplication Level Percentage of deduplicated Percentage of total 1 86.08435573158324 63.81243086951136 2 8.6401692803076 12.809533165808922 3 2.398417158130417 5.3336809388455055 4 1.011686485607377 2.9997633542492363 5 0.5445860487596001 2.01844802656881 6 0.33033000189573114 1.4691979905726282 7 0.21730864369605313 1.127602672953482 8 0.149774152504341 0.8881941599259859 9 0.1094086015501642 0.7299196105093769 >10 0.4796640662965402 6.075046086695704 >50 0.023869525503564044 1.2299084861422223 >100 0.010001864911946323 1.2890810315420986 >500 4.284392535100233E-4 0.21719360667458884 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 5.834235370946519E-5 0.0 0.0 0.0 0.0 38-39 7.778980494595358E-5 0.0 0.0 0.0 0.0 40-41 7.778980494595358E-5 0.0 0.0 0.0 0.0 42-43 7.778980494595358E-5 0.0 0.0 0.0 0.0 44-45 7.778980494595358E-5 0.0 0.0 0.0 0.0 46-47 7.778980494595358E-5 0.0 0.0 0.0 0.0 48-49 7.778980494595358E-5 0.0 0.0 0.0 0.0 50-51 7.778980494595358E-5 0.0 0.0 0.0 0.0 52-53 1.1668470741893038E-4 0.0 0.0 0.0 0.0 54-55 1.1668470741893038E-4 0.0 0.0 0.0 0.0 56-57 1.5557960989190717E-4 0.0 3.3060667102030274E-4 0.0 0.0 58-59 1.9447451236488395E-4 0.0 6.612133420406055E-4 0.0 0.0 60-61 3.6950157349327956E-4 0.0 6.612133420406055E-4 0.0 0.0 62-63 4.083964759662563E-4 0.0 6.612133420406055E-4 0.0 0.0 64-65 5.056337321486983E-4 0.0 6.612133420406055E-4 0.0 0.0 66-67 5.445286346216752E-4 0.0 6.612133420406055E-4 0.0 0.0 68-69 6.028709883311403E-4 0.0 6.612133420406055E-4 0.0 0.0 70-71 6.612133420406055E-4 0.0 6.612133420406055E-4 0.0 0.0 72-73 7.584505982230475E-4 0.0 6.612133420406055E-4 0.0 0.0 74-75 7.778980494595359E-4 0.0 6.612133420406055E-4 0.0 0.0 76-77 8.556878544054894E-4 0.0 6.612133420406055E-4 0.0 0.0 78-79 8.945827568784663E-4 0.0 6.612133420406055E-4 0.0 0.0 80-81 0.0012446368791352574 0.0 6.612133420406055E-4 0.0 0.0 82-83 0.0013613215865541878 0.0 6.612133420406055E-4 0.0 0.0 84-85 0.0013613215865541878 0.0 6.612133420406055E-4 0.0 0.0 86-87 0.0013613215865541878 0.0 6.612133420406055E-4 0.0 0.0 88 0.0013613215865541878 0.0 6.612133420406055E-4 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 9765 0.0 80.176765 8 CACCGGC 9780 0.0 76.1663 9 CTTCACC 10780 0.0 74.76121 6 AGGCGCG 520 0.0 72.21234 5 GGCGCGC 535 0.0 71.06504 6 CCTTCAC 12115 0.0 70.12614 5 GTGTGGC 4035 0.0 67.46977 7 TTCACCG 11985 0.0 67.2837 7 CGTAGTG 1925 0.0 67.05431 4 ACGCAGC 65 1.0913936E-11 64.994896 2 CGGCAGC 25 0.0032607156 63.567524 1 GCAGCCT 75 1.2732926E-11 63.567524 1 GTGCAGC 60 3.6561687E-10 62.587677 2 CGGGGCA 1150 0.0 61.63166 94 GAGCTGC 35 1.6267573E-4 60.5405 1 GCGCGCA 640 0.0 60.139336 7 CAGCGTA 935 0.0 59.48833 1 GCCTTCA 3775 0.0 59.434105 4 CGGGCCC 675 0.0 58.41176 7 TGTGTGG 4460 0.0 57.46224 6 >>END_MODULE