FastQCFastQC Report
Tue 7 Mar 2017
1HL_4A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_4A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2571031
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG97650.080.1767658
CACCGGC97800.076.16639
CTTCACC107800.074.761216
AGGCGCG5200.072.212345
GGCGCGC5350.071.065046
CCTTCAC121150.070.126145
GTGTGGC40350.067.469777
TTCACCG119850.067.28377
CGTAGTG19250.067.054314
ACGCAGC651.0913936E-1164.9948962
CGGCAGC250.003260715663.5675241
GCAGCCT751.2732926E-1163.5675241
GTGCAGC603.6561687E-1062.5876772
CGGGGCA11500.061.6316694
GAGCTGC351.6267573E-460.54051
GCGCGCA6400.060.1393367
CAGCGTA9350.059.488331
GCCTTCA37750.059.4341054
CGGGCCC6750.058.411767
TGTGTGG44600.057.462246
Produced by FastQC (version 0.11.5)