FastQCFastQC Report
Mon 6 Mar 2017
1HL_5A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_5A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1980666
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA106690.5386571991441264No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG58400.2948503180243413No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG49200.2484012953218766No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA44800.22618654533374127No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA39130.19755981068993964No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT39130.19755981068993964No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA38170.19271295614707376No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG37550.18958269592147287No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC35440.1789297135407989No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG35390.17867727320002463No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG34620.17478969195210095No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG32040.1617637703681489No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA29230.14757662321663523No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC28610.14444636299103433No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT28600.14439587492287947No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT28560.14419392265026007No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA28020.141467566969898No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC27790.1403063414023364No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA27330.13798389026721314No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA27260.1376304737901292No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA24720.12480650447879653No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA24510.12374625504754462No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA20600.1040054203989971No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA20170.10183443346833843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTG302.2699169E-886.999653
GCTGCCC1000.086.999653
GCTGCAT257.802937E-786.999653
GCTGCAC257.802937E-786.999653
CTGCAGC402.0008883E-1186.999651
AGCTGCC1150.086.999652
GCAGCTC202.6990629E-586.999653
GCAGCGT356.6393113E-1086.999653
TGCAGCA202.6990629E-586.999652
AGCAGCG1050.086.999642
CAGCTGC2750.085.417841
CAGCCCT120250.081.139381
CAGCAGC2200.081.0678561
AGCTGCT700.080.785392
CAGCGTA42450.080.031481
AGCCCTT102050.079.284342
GCCCTTC93650.077.477523
CAGCGTG32350.077.183611
CAGCTAG29400.077.086421
CAGCTCT44200.077.059651
Produced by FastQC (version 0.11.5)