FastQCFastQC Report
Mon 6 Mar 2017
1HL_5A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_5A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1980666
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG78200.082.351238
CTTCACC87200.076.2739266
CACCGGC80850.075.6461949
CCTTCAC97750.072.987685
GTGTGGC31400.070.7056667
GGCGCGC3500.068.395076
TTCACCG97750.067.4175647
TGCTGCA555.0349627E-967.36051
CAGCTGC555.0349627E-967.36051
AGCTGCA403.973126E-666.157641
CGTAGTG16300.065.655334
CGGGGCA8300.064.4687894
GCAGCCA603.6561687E-1062.5884742
ACCTTCA42600.062.473554
TGTGGCA36400.062.2828458
GCCTTCA28150.062.0279274
CAGCGTA7200.061.747121
CGGGCCC4350.061.5047047
GGCTGCC457.989811E-658.806781
AGGCAGC408.09576E-658.6766932
Produced by FastQC (version 0.11.5)