FastQCFastQC Report
Mon 6 Mar 2017
1HL_6A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_6A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2070051
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA116350.5620634467459981No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG59650.2881571516837025No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA50030.24168486670135178No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA49010.23675745186954333No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT41180.19893229683713107No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG38510.18603406389504415No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG36990.17869124963587854No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG36800.17777339785348284No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG36400.17584107831159715No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT34050.16448870100301877No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC30710.14835383282827333No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC30140.14560027748108623No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA29350.14178394638586198No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT28780.1390303910386749No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA27480.1327503525275464No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA26750.12922386936360505No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC26340.12724324183317223No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA25930.12526261430273938No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA25720.12424814654324941No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA25200.12173613113879803No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC24770.11965888763127092No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA24650.11907919176870521No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG22560.10898282216235253No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA22500.10869297423106968No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGCA402.0008883E-1187.000012
GCAGCCG202.6990372E-587.000013
GCTGCCC950.087.03
GCTGCAT356.6393113E-1087.03
AGCTGCC1400.087.02
GCTGCCA257.8028825E-786.999993
CAGCTGC4950.085.242421
CAGCAGC3900.084.769231
AGCAGCC950.082.421052
AGCAGCA1750.082.028572
AGCAGCG800.081.562512
CAGCCCT123200.081.527191
GCAGCCT650.080.307693
CAGCGTA46850.080.2219851
GCTGCAG600.079.753
GCAGCAT600.079.753
AGCTGCT1750.079.542862
AGCTGCA1150.079.4347842
AGCCCTT103900.078.500962
GCCCTTC93950.078.249073
Produced by FastQC (version 0.11.5)