##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_6A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2070051 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 14.714396891670784 12.0 12.0 12.0 2.0 32.0 2 14.312397134176887 12.0 12.0 12.0 12.0 22.0 3 13.924172399617207 12.0 12.0 12.0 12.0 22.0 4 13.70940715953375 12.0 12.0 12.0 12.0 22.0 5 22.424513695556293 22.0 22.0 22.0 22.0 27.0 6 27.12075789437072 27.0 22.0 32.0 22.0 37.0 7 34.96333375361284 37.0 37.0 37.0 27.0 41.0 8 37.53297624068199 41.0 37.0 41.0 27.0 41.0 9 38.462931106528295 41.0 37.0 41.0 32.0 41.0 10-11 38.83464924294135 41.0 41.0 41.0 34.5 41.0 12-13 39.00398613367497 41.0 41.0 41.0 37.0 41.0 14-15 38.95454049199754 41.0 41.0 41.0 37.0 41.0 16-17 38.85349201541411 41.0 41.0 41.0 37.0 41.0 18-19 38.77836391470548 41.0 41.0 41.0 34.5 41.0 20-21 38.79288432990298 41.0 41.0 41.0 37.0 41.0 22-23 38.78624149839787 41.0 41.0 41.0 34.5 41.0 24-25 38.781503209341224 41.0 41.0 41.0 34.5 41.0 26-27 38.74192326662483 41.0 41.0 41.0 32.0 41.0 28-29 38.696755297333254 41.0 41.0 41.0 32.0 41.0 30-31 38.57411484064885 41.0 41.0 41.0 32.0 41.0 32-33 38.64178177252637 41.0 41.0 41.0 32.0 41.0 34-35 38.59836086164061 41.0 41.0 41.0 32.0 41.0 36-37 38.52022365632537 41.0 41.0 41.0 32.0 41.0 38-39 38.41799163402254 41.0 41.0 41.0 32.0 41.0 40-41 38.46991233549318 41.0 41.0 41.0 32.0 41.0 42-43 38.208000430907255 41.0 41.0 41.0 32.0 41.0 44-45 38.33188361059703 41.0 41.0 41.0 32.0 41.0 46-47 38.33398090191981 41.0 41.0 41.0 32.0 41.0 48-49 38.28177590793656 41.0 41.0 41.0 32.0 41.0 50-51 38.155055116999534 41.0 41.0 41.0 32.0 41.0 52-53 38.123731009525855 41.0 37.0 41.0 32.0 41.0 54-55 37.993318763643984 41.0 37.0 41.0 32.0 41.0 56-57 37.777625285560596 41.0 37.0 41.0 27.0 41.0 58-59 37.75189572624056 41.0 37.0 41.0 27.0 41.0 60-61 37.76410098108694 41.0 37.0 41.0 27.0 41.0 62-63 37.75084840904886 41.0 37.0 41.0 27.0 41.0 64-65 37.7221005666044 41.0 37.0 41.0 27.0 41.0 66-67 37.6095697642232 41.0 37.0 41.0 27.0 41.0 68-69 37.60410975381766 41.0 37.0 41.0 27.0 41.0 70-71 37.57451845389316 41.0 37.0 41.0 27.0 41.0 72-73 37.47151060529427 41.0 37.0 41.0 27.0 41.0 74-75 37.325430146407015 41.0 37.0 41.0 27.0 41.0 76-77 36.188968291119394 39.0 34.5 41.0 24.5 41.0 78-79 36.760431023197015 41.0 37.0 41.0 27.0 41.0 80-81 37.48908239458835 41.0 37.0 41.0 27.0 41.0 82-83 37.6825940520306 41.0 37.0 41.0 27.0 41.0 84-85 37.61115619856709 41.0 37.0 41.0 27.0 41.0 86-87 37.4755010866882 41.0 37.0 41.0 27.0 41.0 88-89 37.3851467910694 41.0 37.0 41.0 27.0 41.0 90-91 37.20157981614946 41.0 37.0 41.0 27.0 41.0 92-93 37.08801787975273 41.0 37.0 41.0 27.0 41.0 94-95 36.91917300588246 41.0 37.0 41.0 27.0 41.0 96-97 36.83195993721894 41.0 37.0 41.0 27.0 41.0 98-99 36.7085992084253 41.0 37.0 41.0 24.5 41.0 100 36.56262140401372 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.8174386295482936 1101 2 -0.5832066063856001 1101 3 -0.10261947269879634 1101 4 0.16949717293436883 1101 5 -1.094755005308766 1101 6 -0.9300660090400292 1101 7 -0.40451751196487606 1101 8 -0.1706183371179435 1101 9 -0.23178097790421504 1101 10-11 -0.06560837295928934 1101 12-13 0.0037590872301578315 1101 14-15 -0.08821982160873176 1101 16-17 -0.1317469671165341 1101 18-19 -0.043471380114695535 1101 20-21 0.04162838010154246 1101 22-23 0.12302375764836881 1101 24-25 0.018920975140403584 1101 26-27 0.05380544332165016 1101 28-29 -0.1660360042671698 1101 30-31 -0.02506557493923367 1101 32-33 -0.12017967059989587 1101 34-35 0.08927603211309076 1101 36-37 -0.13884136398407776 1101 38-39 0.01687450846220173 1101 40-41 -0.043370083078876576 1101 42-43 0.20034697155151804 1101 44-45 0.004551104822802188 1101 46-47 -0.04688572701474669 1101 48-49 -0.03077178707486894 1101 50-51 0.09598136437790572 1101 52-53 -0.17000502099052284 1101 54-55 -0.11914823997482671 1101 56-57 0.04270220786534651 1101 58-59 0.18640942730736043 1101 60-61 -0.0653103317719399 1101 62-63 -0.22123743331811596 1101 64-65 -0.26431895964579866 1101 66-67 -0.04440050155096742 1101 68-69 -0.042715215815455565 1101 70-71 -0.09445707512850277 1101 72-73 -0.16446475878291267 1101 74-75 -0.12494248790584095 1101 76-77 -0.5686198279820687 1101 78-79 -0.5179804824402865 1101 80-81 -0.12372892492913223 1101 82-83 -0.18451478598636584 1101 84-85 -0.24863043829984832 1101 86-87 -0.13836564440497057 1101 88-89 -0.45890397453797505 1101 90-91 -0.2977894323578596 1101 92-93 -0.14493765079876653 1101 94-95 -0.0668786305931306 1101 96-97 -0.03922082751880396 1101 98-99 -0.2940971971177362 1101 100 -0.6360318046552962 1102 1 2.576855244424431 1102 2 0.6736524898837324 1102 3 -0.08163504660751286 1102 4 0.12837593686113102 1102 5 -0.8234467216987866 1102 6 -0.19232050785143784 1102 7 -0.2689676921425743 1102 8 -0.31095896617357255 1102 9 -0.23243595398869843 1102 10-11 -0.20211442822801473 1102 12-13 -0.37193119384348705 1102 14-15 -0.10245488215252863 1102 16-17 -0.1439213721329935 1102 18-19 -0.13014472101729524 1102 20-21 -0.20086377129929645 1102 22-23 -0.1126099643139824 1102 24-25 -0.2920950113143377 1102 26-27 -0.17058847411401956 1102 28-29 -0.24296413086663193 1102 30-31 -0.2857860664143175 1102 32-33 -0.25386325313532865 1102 34-35 -0.1761205736882232 1102 36-37 -0.14720786844784328 1102 38-39 -0.2664591064219337 1102 40-41 0.03167061583098274 1102 42-43 -0.2537144580923396 1102 44-45 -0.26776112968589416 1102 46-47 -0.5213410669814209 1102 48-49 -0.29792941907255965 1102 50-51 -0.3482254907025606 1102 52-53 -0.24207150610137518 1102 54-55 -0.18851983932268723 1102 56-57 -0.026600041073578495 1102 58-59 -0.16467998167006925 1102 60-61 -0.1195820650490802 1102 62-63 -0.2146974526591734 1102 64-65 -0.25165999087452207 1102 66-67 -0.27093613150903906 1102 68-69 -0.30516383649806045 1102 70-71 -0.2443187151352717 1102 72-73 -0.24491049223610162 1102 74-75 -0.45050249986730506 1102 76-77 -0.2034361338636046 1102 78-79 -0.34749450500407164 1102 80-81 -0.4258366438963819 1102 82-83 -0.6454876951678585 1102 84-85 -0.42535395155600497 1102 86-87 -0.23544821495789847 1102 88-89 -0.25302883906017115 1102 90-91 -0.4614152021399889 1102 92-93 -0.3416008884729891 1102 94-95 -0.2944306589592216 1102 96-97 -0.17979188368107657 1102 98-99 -0.3970235769167303 1102 100 -0.7688019771036849 1103 1 2.0727719501252135 1103 2 0.4034246703402289 1103 3 0.6946564859230371 1103 4 0.8960023616063069 1103 5 2.6605924384422757 1103 6 0.020083742164313634 1103 7 -0.6680158495306046 1103 8 -0.26289004511995273 1103 9 -0.08823850314141168 1103 10-11 -0.21564568547415774 1103 12-13 -0.21354006984413587 1103 14-15 -0.35633415918174904 1103 16-17 -0.14776720243595065 1103 18-19 -0.32326239358455666 1103 20-21 -0.3116865110999072 1103 22-23 -0.2924909591546552 1103 24-25 -0.3178493009539878 1103 26-27 -0.2749141679357052 1103 28-29 -0.11159982415750846 1103 30-31 -0.14820780805906963 1103 32-33 -0.28137337950031593 1103 34-35 -0.4371051459783146 1103 36-37 -0.3390241451924254 1103 38-39 -0.17910184478844826 1103 40-41 -0.3071417724018488 1103 42-43 -0.22213593712985613 1103 44-45 -0.636910707481114 1103 46-47 -0.4801227494122813 1103 48-49 -0.21627284540176106 1103 50-51 -0.09480267224394368 1103 52-53 -0.18869316946062042 1103 54-55 -0.2086864304044127 1103 56-57 -0.2743799079137048 1103 58-59 -0.4645504473881914 1103 60-61 -0.2968301584304811 1103 62-63 -0.26897877330664954 1103 64-65 -0.49429480446217866 1103 66-67 -0.5949036054601677 1103 68-69 -0.5176871289223968 1103 70-71 -0.3766212374374831 1103 72-73 -0.44969137194553355 1103 74-75 -0.3518964290459152 1103 76-77 -0.5990871592579694 1103 78-79 -0.6127501699172271 1103 80-81 -0.3465165297025763 1103 82-83 -0.29691429504661215 1103 84-85 -0.3506179789267563 1103 86-87 -0.49738310884873727 1103 88-89 -0.3916125168715041 1103 90-91 -0.5237041812341658 1103 92-93 -0.41645931802580094 1103 94-95 -0.32862159429110704 1103 96-97 -0.09716046598376238 1103 98-99 -0.38937987472576197 1103 100 -0.3235169411534926 1104 1 2.5418440712135695 1104 2 -0.6253845026959262 1104 3 -0.08277668306776498 1104 4 -0.09080343705950433 1104 5 -1.108588960705866 1104 6 -0.5565950557040935 1104 7 -0.20163100842746928 1104 8 -0.12193836571430694 1104 9 -0.06840799961145194 1104 10-11 -0.11422813996391312 1104 12-13 -0.13309610189579502 1104 14-15 -0.22030986882058556 1104 16-17 -0.16045953303672178 1104 18-19 -0.22788659375181197 1104 20-21 -0.060029866653515285 1104 22-23 -0.0925493360047227 1104 24-25 -0.12693861601514556 1104 26-27 -0.2509995640595548 1104 28-29 -0.0631168017621917 1104 30-31 0.012397664109670359 1104 32-33 0.018560859175600797 1104 34-35 -0.3381650459141312 1104 36-37 -0.28950410561407836 1104 38-39 -0.4039675871499284 1104 40-41 -0.3173955551974288 1104 42-43 -0.22428974204846241 1104 44-45 -0.12270228055033527 1104 46-47 -0.38834027109771796 1104 48-49 -0.4816126466834305 1104 50-51 -0.3281851746264195 1104 52-53 -0.2947685555197026 1104 54-55 -0.21110066718407694 1104 56-57 -0.2453961513254157 1104 58-59 -0.30797270437518165 1104 60-61 -0.22226900283018836 1104 62-63 -0.2934238133712199 1104 64-65 -0.540214685046422 1104 66-67 -0.8714225279468977 1104 68-69 -0.8161374833771546 1104 70-71 -0.3517550088554984 1104 72-73 -0.20714619598233242 1104 74-75 -0.414988858349453 1104 76-77 -0.47400723676151557 1104 78-79 -0.5429094557565364 1104 80-81 -0.6071129460143041 1104 82-83 -0.6088290071068272 1104 84-85 -0.4001135555356612 1104 86-87 -0.4506959115210094 1104 88-89 -0.6017302794792059 1104 90-91 -0.5586233227632249 1104 92-93 -0.48090663117126553 1104 94-95 -0.3215454144770362 1104 96-97 -0.4892331875544542 1104 98-99 -0.5632784637138357 1104 100 -0.26108896256664593 1105 1 1.5132874641557787 1105 2 0.4460465226256307 1105 3 -0.0020378780547556374 1105 4 -0.19291074220076965 1105 5 -1.1070315566236353 1105 6 0.2872282338997749 1105 7 0.04822756554006702 1105 8 -0.27675578298866554 1105 9 -0.06680020892656557 1105 10-11 -0.028170971782600418 1105 12-13 -0.26240415642325843 1105 14-15 -0.17431004975428976 1105 16-17 -0.17252111233607081 1105 18-19 -0.29566802583067897 1105 20-21 -0.25064438853046056 1105 22-23 -0.2079584179608176 1105 24-25 -0.2566448964761392 1105 26-27 -0.31683624504822916 1105 28-29 -0.358358531488463 1105 30-31 -0.3852113198215932 1105 32-33 -0.3795104790925663 1105 34-35 -0.30032757019131395 1105 36-37 -0.28961117714472806 1105 38-39 -0.2147550152344948 1105 40-41 -0.17100987781825694 1105 42-43 -0.26238229994725515 1105 44-45 -0.17262366599868528 1105 46-47 -0.3136281045654101 1105 48-49 -0.2220492122409894 1105 50-51 -0.3740890873751397 1105 52-53 -0.39953318137627747 1105 54-55 -0.24237213874770447 1105 56-57 -0.2605582244134723 1105 58-59 -0.26745300092323276 1105 60-61 -0.32360877114342657 1105 62-63 -0.46674857334776476 1105 64-65 -0.2690683440298969 1105 66-67 -0.344973283287878 1105 68-69 -0.5019111696610068 1105 70-71 -0.4769628535914734 1105 72-73 -0.4408097715072614 1105 74-75 -0.5143412187327527 1105 76-77 -0.8167729802845471 1105 78-79 -0.3494340627410395 1105 80-81 -0.5413598609800445 1105 82-83 -0.3374479103279029 1105 84-85 -0.3911295670238033 1105 86-87 -0.496163522096694 1105 88-89 -0.3379472533514587 1105 90-91 -0.2278146635965328 1105 92-93 -0.26610625372980223 1105 94-95 -0.4095785390876827 1105 96-97 -0.3829649796684933 1105 98-99 -0.4683741524528955 1105 100 -0.6861635118355807 1106 1 1.3914692432502935 1106 2 -0.5842459259345532 1106 3 -0.05002784254816639 1106 4 -0.10513492119441814 1106 5 -1.1133022956731544 1106 6 -0.6859224863709379 1106 7 -0.09090033545750487 1106 8 0.03539629049504356 1106 9 -0.07039938363513443 1106 10-11 -0.14334049526453185 1106 12-13 -0.02581470637157679 1106 14-15 0.05122491676980445 1106 16-17 0.03165673668858915 1106 18-19 0.004811931917011236 1106 20-21 -0.11090074366375546 1106 22-23 -0.06828435113452969 1106 24-25 -0.1582420829086857 1106 26-27 -0.16358472813074343 1106 28-29 -0.17724151778376296 1106 30-31 -0.11041844159158387 1106 32-33 -0.13490473819246773 1106 34-35 -0.2148562494170818 1106 36-37 -0.22694489434355347 1106 38-39 -0.09511128966307325 1106 40-41 -0.13388716074784668 1106 42-43 0.007607369384196261 1106 44-45 -0.22377253162969168 1106 46-47 -0.32884840984887376 1106 48-49 -0.16598107747282143 1106 50-51 -0.18864777667012333 1106 52-53 -0.12228023280709266 1106 54-55 -0.23975170482476926 1106 56-57 -0.3044249274137272 1106 58-59 -0.41093918902856075 1106 60-61 -0.06740339768511205 1106 62-63 -0.38109626251434037 1106 64-65 -0.4563632776446056 1106 66-67 -0.49283170316041236 1106 68-69 -0.41899099528303196 1106 70-71 -0.5765560472031908 1106 72-73 -0.21097592793496744 1106 74-75 -0.5451884032769669 1106 76-77 -0.2518745351721847 1106 78-79 -0.13826964881474169 1106 80-81 -0.2254242818205725 1106 82-83 -0.29099999275943134 1106 84-85 -0.6440785719851974 1106 86-87 -0.39898856484579426 1106 88-89 -0.4367046389369804 1106 90-91 -0.6602911363413995 1106 92-93 -0.621501878244274 1106 94-95 -0.29351800996919764 1106 96-97 -0.3507256532885066 1106 98-99 -0.6862853121895469 1106 100 -0.6442168141343032 1107 1 1.0159938438547211 1107 2 -0.5263057796947894 1107 3 -0.19457329163161496 1107 4 -0.2235596894278249 1107 5 -1.270582139940423 1107 6 -0.2635839719602515 1107 7 -0.2684614839863926 1107 8 -0.29701888863965564 1107 9 -0.1992814365193709 1107 10-11 -0.16770542776232134 1107 12-13 -0.12350440049374356 1107 14-15 -0.24942186673646205 1107 16-17 -0.35551033352933104 1107 18-19 -0.27020849598750374 1107 20-21 -0.25896234581229294 1107 22-23 -0.18979793869513628 1107 24-25 -0.27858992806516625 1107 26-27 -0.29018876925091774 1107 28-29 -0.3189659633764421 1107 30-31 -0.5903537331999189 1107 32-33 -0.27205348337839297 1107 34-35 -0.3355776548569196 1107 36-37 -0.3065950965197217 1107 38-39 -0.27849273872248403 1107 40-41 -0.39577514974080685 1107 42-43 -0.0722642352276992 1107 44-45 -0.19805575250941132 1107 46-47 -0.3220870738639121 1107 48-49 -0.34156492500855506 1107 50-51 -0.40696619963588887 1107 52-53 -0.3665772425223608 1107 54-55 -0.5782455374000719 1107 56-57 -0.28602884836974596 1107 58-59 -0.6568376863759369 1107 60-61 -0.29781904545997406 1107 62-63 -0.3581512195857002 1107 64-65 -0.39553393476222 1107 66-67 -0.3184425876285957 1107 68-69 -0.7220855224070917 1107 70-71 -0.6434481217770553 1107 72-73 -0.41200303095769897 1107 74-75 -0.5639888346662403 1107 76-77 -0.50035836408248 1107 78-79 -0.898707106381778 1107 80-81 -0.5731506260067292 1107 82-83 -0.603474232571017 1107 84-85 -0.7000857277599835 1107 86-87 -0.40421405286242873 1107 88-89 -0.47987541021053914 1107 90-91 -0.463165763081534 1107 92-93 -0.3540924970295407 1107 94-95 -0.41604202777752874 1107 96-97 -0.5204014804684363 1107 98-99 -0.7840510101329343 1107 100 -0.8887379299785465 1108 1 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0.09828454495093553 2223 22-23 0.28935295861322885 2223 24-25 0.17620753745806184 2223 26-27 0.2887711299821518 2223 28-29 0.16032693173059442 2223 30-31 0.2406989045747281 2223 32-33 0.3476702315783129 2223 34-35 0.2964567542262344 2223 36-37 0.24160881526211853 2223 38-39 0.2321017685377953 2223 40-41 0.2795612305366504 2223 42-43 0.47900653470937726 2223 44-45 0.4045633343490991 2223 46-47 0.34191788207998286 2223 48-49 0.2984228322796838 2223 50-51 0.3715737392682712 2223 52-53 0.3833485948967805 2223 54-55 0.42752398766511845 2223 56-57 0.531242939831337 2223 58-59 0.5976211916501555 2223 60-61 0.5633200564664875 2223 62-63 0.5670749468563301 2223 64-65 0.4114307261794039 2223 66-67 0.5871093055772931 2223 68-69 0.4560621199880117 2223 70-71 0.5943215268924291 2223 72-73 0.515057187880771 2223 74-75 0.521153192562025 2223 76-77 0.6817766942175538 2223 78-79 0.623787993590561 2223 80-81 0.595932259506263 2223 82-83 0.44819648556998004 2223 84-85 0.3769864100646956 2223 86-87 0.45609057288140775 2223 88-89 0.4727276375183749 2223 90-91 0.5621875392133902 2223 92-93 0.5457877245164582 2223 94-95 0.7617852734696982 2223 96-97 0.5773990987060102 2223 98-99 0.7345607075548415 2223 100 0.6746595991126085 2224 1 1.082919729470703 2224 2 -0.8367073057512684 2224 3 -0.10442595228299645 2224 4 -0.17636194574445874 2224 5 -1.0685610513378663 2224 6 0.3833504567633135 2224 7 0.09830231664322042 2224 8 0.3563515428457933 2224 9 0.3232641754831107 2224 10-11 0.2743831279843789 2224 12-13 0.2657760474927713 2224 14-15 0.2487565788860735 2224 16-17 0.24663480567650709 2224 18-19 0.19868376723383108 2224 20-21 0.1730084788901678 2224 22-23 0.2051200825807129 2224 24-25 0.2787843536701331 2224 26-27 0.2929321566815162 2224 28-29 0.26480593565104726 2224 30-31 0.3025876704443533 2224 32-33 0.18969738556097582 2224 34-35 0.27534816054173916 2224 36-37 0.2643700405795997 2224 38-39 0.30906914029306876 2224 40-41 0.16079221792879395 2224 42-43 0.3260874131239504 2224 44-45 0.16899623171509148 2224 46-47 0.05989399086475089 2224 48-49 0.1723556591995674 2224 50-51 0.2843812707850617 2224 52-53 0.3487827967993411 2224 54-55 0.2415747647684796 2224 56-57 0.3562920096859372 2224 58-59 0.266301847146984 2224 60-61 0.3138809159204996 2224 62-63 0.41403218806097186 2224 64-65 0.28563407188501344 2224 66-67 0.2886721825852163 2224 68-69 0.42874713801023745 2224 70-71 0.5649914152567277 2224 72-73 0.2370537163418618 2224 74-75 0.16411360070456738 2224 76-77 0.45075165475916634 2224 78-79 0.2379469211668166 2224 80-81 0.16267981086205197 2224 82-83 0.10599164766163938 2224 84-85 0.2930127871436454 2224 86-87 0.26333003776473163 2224 88-89 0.23640915719224154 2224 90-91 0.3557045572264883 2224 92-93 0.2414984402546878 2224 94-95 0.4359928117173766 2224 96-97 0.20288872772592015 2224 98-99 0.19105139012921057 2224 100 0.7197870381310878 2225 1 1.4021354799963586 2225 2 1.638605697716688 2225 3 0.8923467327884111 2225 4 1.0251429967748606 2225 5 3.2803183484600638 2225 6 0.03978904391276217 2225 7 0.22776867474376417 2225 8 0.42273080611003877 2225 9 0.3220097722718336 2225 10-11 0.16413217907528121 2225 12-13 0.19422301098367 2225 14-15 0.24638857282398163 2225 16-17 0.06609375682475616 2225 18-19 0.22074846371944545 2225 20-21 0.2949219643220147 2225 22-23 0.2787689111217162 2225 24-25 0.34436489488965805 2225 26-27 0.24011495480281297 2225 28-29 0.21629807813759783 2225 30-31 0.13867557082937765 2225 32-33 0.08679877547386639 2225 34-35 0.17766257713331868 2225 36-37 0.21583316965854493 2225 38-39 0.11128900730926006 2225 40-41 0.10506428859863348 2225 42-43 0.27614083192737127 2225 44-45 0.261086494132833 2225 46-47 0.3359104841336418 2225 48-49 0.34711791992428687 2225 50-51 0.3261076709379793 2225 52-53 0.21483301388789755 2225 54-55 0.35678956638503934 2225 56-57 0.4287376351467387 2225 58-59 0.521130872842626 2225 60-61 0.4684660583251343 2225 62-63 0.28285915892468694 2225 64-65 0.3403482386199457 2225 66-67 0.5252005747645683 2225 68-69 0.4471757718542051 2225 70-71 0.4416938196148834 2225 72-73 0.33940875170408447 2225 74-75 0.4059821665547645 2225 76-77 0.5772057384816307 2225 78-79 0.48470253952786635 2225 80-81 0.31853130317519884 2225 82-83 0.27350226315547843 2225 84-85 0.0910009633267066 2225 86-87 0.2688323451839736 2225 88-89 0.34974150065637133 2225 90-91 0.47684492067915585 2225 92-93 0.46423778380074054 2225 94-95 0.7164671297316332 2225 96-97 0.56646512514817 2225 98-99 0.5894532633713396 2225 100 0.6014635352229192 2226 1 0.8395573553567104 2226 2 1.4685318165999028 2226 3 0.9105013423151185 2226 4 0.9749630506491513 2226 5 3.239997674201181 2226 6 -0.2691656541744223 2226 7 0.14066835002201827 2226 8 0.13450854237509446 2226 9 0.4454348833183701 2226 10-11 0.09577686706104771 2226 12-13 0.24849037483345882 2226 14-15 0.3431690016504163 2226 16-17 0.2891045766549212 2226 18-19 0.2195623241260023 2226 20-21 0.22446117885516514 2226 22-23 0.2749856317549231 2226 24-25 0.31176414581561573 2226 26-27 0.2138851115364062 2226 28-29 0.13513623501879124 2226 30-31 0.18277419122320282 2226 32-33 0.12861131323891328 2226 34-35 0.11986288409684676 2226 36-37 0.3317359733817753 2226 38-39 0.3332092532952089 2226 40-41 0.3093740588487677 2226 42-43 0.40568806072244 2226 44-45 0.2607685182009831 2226 46-47 0.254269947420255 2226 48-49 0.20288037604764497 2226 50-51 0.42220241911718404 2226 52-53 0.20466066689687779 2226 54-55 0.38913302305728337 2226 56-57 0.44348892204116197 2226 58-59 0.45446504219608386 2226 60-61 0.5716237351808644 2226 62-63 0.5095171886385685 2226 64-65 0.3075915285494233 2226 66-67 0.4883509695967021 2226 68-69 0.5650090613685563 2226 70-71 0.32557538035491973 2226 72-73 0.35665397312833846 2226 74-75 0.3657190047151033 2226 76-77 0.677955389082868 2226 78-79 0.48251995042542006 2226 80-81 0.3327508524392684 2226 82-83 0.30517286776523633 2226 84-85 0.4016066570273793 2226 86-87 0.55929226003677 2226 88-89 0.42596246481244293 2226 90-91 0.5816326926451509 2226 92-93 0.49470956437370717 2226 94-95 0.6389248213474374 2226 96-97 0.6335305157598299 2226 98-99 0.6301328497201126 2226 100 0.6157629388234582 2227 1 1.0777994915549893 2227 2 1.5409008884998041 2227 3 0.7255844869235304 2227 4 -0.011143675145730114 2227 5 3.2967632479318567 2227 6 0.3052101318503162 2227 7 0.5985051388529001 2227 8 0.5819892053519737 2227 9 0.5891348672646615 2227 10-11 0.4100556969526963 2227 12-13 0.46171019281634784 2227 14-15 0.3593271230224033 2227 16-17 0.3374252161021545 2227 18-19 0.32227380217535995 2227 20-21 0.35929044969078205 2227 22-23 0.3556647215864075 2227 24-25 0.40364158904559844 2227 26-27 0.4774425017832158 2227 28-29 0.35242249997141073 2227 30-31 0.6511759226546445 2227 32-33 0.41691408034218824 2227 34-35 0.46190367829949963 2227 36-37 0.40037285320344296 2227 38-39 0.3673670820275632 2227 40-41 0.4518578703675118 2227 42-43 0.6150555799735642 2227 44-45 0.47854676133240304 2227 46-47 0.42738036319957473 2227 48-49 0.5207527792410929 2227 50-51 0.47668478165504524 2227 52-53 0.5565925624257133 2227 54-55 0.5445566604021295 2227 56-57 0.647054890065256 2227 58-59 0.699821461282113 2227 60-61 0.6295111435606628 2227 62-63 0.5182097410782305 2227 64-65 0.46060622036256404 2227 66-67 0.5780743422033723 2227 68-69 0.6183657387357187 2227 70-71 0.7531972745390334 2227 72-73 0.5569478864015593 2227 74-75 0.6319662804710902 2227 76-77 0.7722330401225364 2227 78-79 0.8739590858233797 2227 80-81 0.6514488963150811 2227 82-83 0.6802207771393611 2227 84-85 0.5083161612401526 2227 86-87 0.5559405291088453 2227 88-89 0.5970802356803375 2227 90-91 0.49761697777805125 2227 92-93 0.5762231807065561 2227 94-95 0.8369883940509197 2227 96-97 0.7616557323516702 2227 98-99 0.8842699389509079 2227 100 1.0940927958269313 2228 1 1.121696884722894 2228 2 1.5213836721635836 2228 3 -0.35530914484174936 2228 4 -0.10997186955954952 2228 5 3.3060256053191885 2228 6 0.29983160542392184 2228 7 0.5513966762615894 2228 8 0.5020943154275272 2228 9 0.12728912340256926 2228 10-11 0.27148806688596494 2228 12-13 0.30910163721515715 2228 14-15 0.26933768989531615 2228 16-17 0.21860212683110092 2228 18-19 0.08498213092753559 2228 20-21 0.2011968371812145 2228 22-23 0.3666422643070604 2228 24-25 0.441956160929756 2228 26-27 0.38505561278827116 2228 28-29 0.37927011294571855 2228 30-31 0.2555612343447109 2228 32-33 0.2655941424121693 2228 34-35 0.293224917773486 2228 36-37 0.2944014705299054 2228 38-39 0.37267889689690037 2228 40-41 0.4358325360222608 2228 42-43 0.5291193568164303 2228 44-45 0.4353454564602899 2228 46-47 0.47969990472328305 2228 48-49 0.43966332156890076 2228 50-51 0.4801010374042747 2228 52-53 0.35429860604704544 2228 54-55 0.3764462110247635 2228 56-57 0.5542855610973376 2228 58-59 0.6167113417480792 2228 60-61 0.3964368610590583 2228 62-63 0.5352437719955461 2228 64-65 0.47546194807718933 2228 66-67 0.5941870404585288 2228 68-69 0.5256565838894289 2228 70-71 0.6169941160068149 2228 72-73 0.5747515517633133 2228 74-75 0.5906716780749406 2228 76-77 0.6135727508231881 2228 78-79 0.45972295840995514 2228 80-81 0.5066947722333666 2228 82-83 0.5191820499661617 2228 84-85 0.6187075931583976 2228 86-87 0.4475305344398208 2228 88-89 0.5178928745636995 2228 90-91 0.4831650852796372 2228 92-93 0.6525261955934809 2228 94-95 0.7871465405572238 2228 96-97 0.6680182616735522 2228 98-99 0.8629118402273122 2228 100 0.8896526291104436 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 16.0 13 25.0 14 88.0 15 409.0 16 1386.0 17 3603.0 18 5852.0 19 7501.0 20 8831.0 21 10387.0 22 11799.0 23 13601.0 24 15469.0 25 16989.0 26 19414.0 27 21675.0 28 24705.0 29 28075.0 30 31659.0 31 36998.0 32 43194.0 33 50655.0 34 63528.0 35 81558.0 36 115243.0 37 189067.0 38 458302.0 39 809099.0 40 923.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 19.373255691695363 14.907063743828356 40.29820011080767 25.42148045366861 2 25.454411062778874 48.27685160671945 9.97873360091056 16.290003729591117 3 31.35806798962924 13.175279256404792 34.20282881919334 21.26382393477262 4 19.596763557999296 16.81118001440544 25.96385306448972 37.62820336310554 5 17.2370149334485 35.282270823279234 26.01670200396029 21.464012239311977 6 16.93025920617415 27.82240630786391 31.147348543586606 24.099985942375334 7 20.219936610257427 26.61132503498706 36.77320027380968 16.395538080945833 8 20.373894169757172 27.78574054455663 34.72643910705582 17.113926178630383 9 20.715576572751107 27.957427135853173 28.060709615366964 23.266286676028756 10-11 19.296360331218892 30.151914131584196 28.852815703574453 21.698909833622455 12-13 24.097908698867805 26.647700950363056 27.36205533100392 21.892335019765213 14-15 21.8482056722274 27.352055577374664 28.435507144509963 22.364231605887973 16-17 20.86697380885785 29.817188078941047 28.14766399475182 21.168174117449283 18-19 21.483045586799552 29.488645448831935 29.862742512141004 19.165566452227505 20-21 22.870402709884928 28.91131667770504 29.9655660657636 18.252714546646434 22-23 22.10604956109777 30.38983097517887 28.57968233632891 18.924437127394445 24-25 22.207882800955144 28.299471848761215 26.56804107724882 22.924604273034817 26-27 20.05578606517424 26.412972434012495 30.112518966924 23.418722533889262 28-29 19.627384281909325 28.057432664997293 30.691123825295293 21.62405922779809 30-31 20.006841101881207 30.243564484632163 28.025255067778122 21.72433934570851 32-33 20.565773500266417 30.214859440661122 28.855593412915916 20.363773646156545 34-35 21.417612416312448 29.135828054477887 29.961773888662645 19.48478564054702 36-37 21.904581094861914 28.301935556177117 28.717215179722626 21.076268169238343 38-39 21.546420189891336 29.241540927505262 28.918670586746252 20.293368295857153 40-41 20.760042801622212 30.046932027062606 29.73599225126388 19.457032920051304 42-43 20.55226712024691 30.007816240098666 29.18699025965086 20.252926380003565 44-45 20.512030863007723 30.16024725960858 28.827067545678826 20.500654331704872 46-47 19.795570393963104 27.947319895835925 29.11405705352672 23.14305265667425 48-49 20.651810027868876 28.239135171065833 30.396642401564016 20.712412399501268 50-51 22.04184824431862 30.705233832403163 28.08822101484456 19.16469690843366 52-53 20.417090472821535 30.334697967010634 28.29354446709843 20.9546670930694 54-55 22.648155762556513 28.241554301352444 28.16794328352007 20.942346652570972 56-57 21.559990723831056 28.907392913393437 29.14654414393522 20.386072218840283 58-59 21.330660580985437 27.945094554895178 28.90874932631615 21.815495537803237 60-61 19.54945478304242 30.4204855202598 29.656082771047526 20.373976925650254 62-63 20.32453726999382 31.309587336219753 27.365768379055353 21.00010701473107 64-65 19.847646118521833 30.418507001525086 28.764077565313674 20.96976931463941 66-67 20.176652652519188 30.37992783752671 29.404903550685468 20.038515959268636 68-69 20.788618251434386 30.131214158491748 29.23292711145764 19.847240478616225 70-71 19.674732651514383 30.951387188045125 29.334639581343648 20.03924057909684 72-73 19.922504324772675 30.98199029878974 28.559948523007407 20.53555685343018 74-75 19.93598225357733 29.27031749459313 30.526011194893265 20.267689056936277 76-77 19.476090202608535 30.427921824148296 28.768083491662765 21.327904481580408 78-79 19.492804766645847 29.52417597440836 28.501930628762285 22.481088630183507 80-81 20.863665754691255 29.46952692171222 28.455202861189 21.211604462407525 82-83 20.13476962644882 28.140900876355218 30.01805269532007 21.706276801875894 84-85 20.67009460153397 29.091408859008787 28.406353273421765 21.832143266035477 86-87 21.14071102596023 28.882380192565304 29.512316363220037 20.46459241825443 88-89 20.897673535579557 29.001700924276747 29.02073427176432 21.079891268379377 90-91 21.134382679460554 29.165682391400015 28.435772838446976 21.26416209069245 92-93 21.309619907915312 29.0488978290873 28.974020446839234 20.667461816158152 94-95 21.72895740249878 28.79221333194206 28.641299175720793 20.837530089838367 96-97 21.06293516439933 29.27316766591741 28.682409273974407 20.98148789570885 98-99 22.362589134277368 29.573570892697816 27.973392926068 20.090447046956815 100 21.35599557692057 31.058220304717132 27.639319031270244 19.946465087092058 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 4.0 1 8.0 2 14.0 3 19.5 4 22.0 5 22.0 6 24.5 7 28.5 8 44.5 9 59.0 10 71.5 11 80.5 12 86.5 13 99.0 14 111.0 15 144.5 16 207.0 17 287.5 18 415.0 19 690.5 20 1118.0 21 1640.0 22 2394.0 23 3706.5 24 5650.0 25 8567.0 26 12464.5 27 16535.5 28 20791.0 29 26044.0 30 32224.0 31 38882.0 32 46554.5 33 55117.5 34 63992.0 35 73637.0 36 82878.5 37 90011.5 38 95328.0 39 99186.5 40 101078.5 41 100333.5 42 98372.0 43 97073.0 44 96248.0 45 92469.0 46 85042.5 47 77899.5 48 73228.5 49 70792.5 50 67924.5 51 61742.0 52 53234.0 53 44784.0 54 36800.5 55 29429.0 56 22576.0 57 16558.5 58 11885.0 59 8651.5 60 6845.5 61 5908.5 62 4971.0 63 3818.5 64 2886.0 65 2666.0 66 3162.5 67 2977.0 68 2108.5 69 2022.0 70 2423.5 71 2143.0 72 1176.5 73 456.5 74 237.0 75 163.5 76 111.0 77 84.0 78 68.0 79 62.0 80 44.5 81 29.0 82 25.0 83 22.0 84 19.0 85 16.0 86 15.5 87 18.0 88 15.5 89 12.5 90 12.0 91 12.0 92 14.0 93 12.5 94 7.5 95 6.0 96 10.5 97 12.0 98 25.5 99 63.5 100 65.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.325228219014893 2 0.005748650637109907 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 4.5892589119784973E-4 30-31 0.010096369606352695 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.001207699713678552 40-41 0.0017390875876971148 42-43 9.661597709428414E-5 44-45 0.0 46-47 0.0022946294559892484 48-49 0.0 50-51 0.0 52-53 9.903137652164124E-4 54-55 0.018743499556291125 56-57 0.01115914535438982 58-59 0.0132605428561905 60-61 1.6907795991499727E-4 62-63 0.011714687222681952 64-65 9.661597709428414E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.2076997136785519E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2070051.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 78.30645570703585 #Duplication Level Percentage of deduplicated Percentage of total 1 87.64843508032143 68.63438299408197 2 8.266877288052557 12.946997203847765 3 2.002032691856615 4.703162529267233 4 0.7771147684728611 2.4341241278681407 5 0.4054092285672735 1.587307990001339 6 0.21896945331326903 1.0288033078241614 7 0.14137570773717392 0.77494414171803 8 0.09500254235733921 0.5951449900128625 9 0.06849952618303251 0.4827559601704121 >10 0.3501881707024652 4.813124467438246 >50 0.019456437451515103 1.0557173227552723 >100 0.00651398320242351 0.8891569348079508 >500 1.2512178200410395E-4 0.05437803020671815 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 2.415399427357104E-5 0.0 0.0 0.0 14-15 0.0 4.830798854714208E-5 0.0 0.0 0.0 16-17 0.0 4.830798854714208E-5 0.0 0.0 0.0 18-19 0.0 4.830798854714208E-5 0.0 0.0 0.0 20-21 0.0 4.830798854714208E-5 0.0 0.0 0.0 22-23 0.0 7.246198282071312E-5 0.0 0.0 0.0 24-25 0.0 9.661597709428415E-5 0.0 0.0 0.0 26-27 0.0 9.661597709428415E-5 0.0 0.0 0.0 28-29 0.0 9.661597709428415E-5 0.0 0.0 0.0 30-31 0.0 9.661597709428415E-5 0.0 0.0 0.0 32-33 0.0 9.661597709428415E-5 0.0 0.0 0.0 34-35 0.0 9.661597709428415E-5 0.0 0.0 0.0 36-37 0.0 9.661597709428415E-5 0.0 0.0 0.0 38-39 0.0 9.661597709428415E-5 0.0 0.0 0.0 40-41 0.0 9.661597709428415E-5 0.0 0.0 0.0 42-43 0.0 9.661597709428415E-5 0.0 0.0 0.0 44-45 0.0 9.661597709428415E-5 0.0 0.0 0.0 46-47 0.0 9.661597709428415E-5 0.0 0.0 0.0 48-49 0.0 9.661597709428415E-5 0.0 0.0 0.0 50-51 0.0 9.661597709428415E-5 0.0 0.0 0.0 52-53 0.0 9.661597709428415E-5 0.0 0.0 0.0 54-55 4.830798854714208E-5 9.661597709428415E-5 0.0 0.0 0.0 56-57 7.246198282071312E-5 9.661597709428415E-5 0.0 0.0 0.0 58-59 1.6907795991499727E-4 9.661597709428415E-5 0.0 0.0 0.0 60-61 1.932319541885683E-4 9.661597709428415E-5 0.0 0.0 0.0 62-63 2.8984793128285246E-4 9.661597709428415E-5 0.0 0.0 0.0 64-65 2.8984793128285246E-4 9.661597709428415E-5 0.0 0.0 0.0 66-67 2.8984793128285246E-4 9.661597709428415E-5 0.0 0.0 0.0 68-69 3.3815591982999454E-4 9.661597709428415E-5 0.0 0.0 0.0 70-71 3.3815591982999454E-4 9.661597709428415E-5 0.0 0.0 0.0 72-73 3.864639083771366E-4 9.661597709428415E-5 0.0 0.0 0.0 74-75 4.347718969242787E-4 9.661597709428415E-5 0.0 0.0 0.0 76-77 4.347718969242787E-4 9.661597709428415E-5 0.0 0.0 0.0 78-79 5.796958625657049E-4 9.661597709428415E-5 0.0 0.0 0.0 80-81 7.970818110278443E-4 9.661597709428415E-5 0.0 0.0 0.0 82-83 9.661597709428415E-4 9.661597709428415E-5 0.0 0.0 0.0 84-85 0.0010627757480371257 9.661597709428415E-5 0.0 0.0 0.0 86-87 0.0011110837365842677 1.4492396564142623E-4 0.0 0.0 0.0 88 0.0011110837365842677 1.4492396564142623E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 8575 0.0 80.90246 8 CACCGGC 8275 0.0 79.63804 9 CTTCACC 9375 0.0 76.65233 6 CCTTCAC 10325 0.0 73.00909 5 GGCGCGC 380 0.0 70.40649 6 GTGTGGC 3475 0.0 68.346634 7 TTCACCG 10530 0.0 67.39767 7 CGTAGTG 1540 0.0 67.35859 4 CGGGCCC 540 0.0 64.321976 7 GCTGCCA 95 0.0 64.23203 2 CGGGGCA 845 0.0 63.32418 94 AACGGGC 550 0.0 63.15249 5 AGGCGCG 420 0.0 62.58354 5 GCCTTCA 3085 0.0 62.22853 4 ACCTTCA 4035 0.0 61.18763 4 ATGCAGC 95 0.0 59.291103 2 GCGTAGT 980 0.0 58.91155 3 TGTGGCA 4005 0.0 58.481632 8 GGGCCCG 595 0.0 57.587376 8 GGCCCGA 605 0.0 56.635525 9 >>END_MODULE