FastQCFastQC Report
Mon 6 Mar 2017
1HL_6A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_6A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2070051
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG85750.080.902468
CACCGGC82750.079.638049
CTTCACC93750.076.652336
CCTTCAC103250.073.009095
GGCGCGC3800.070.406496
GTGTGGC34750.068.3466347
TTCACCG105300.067.397677
CGTAGTG15400.067.358594
CGGGCCC5400.064.3219767
GCTGCCA950.064.232032
CGGGGCA8450.063.3241894
AACGGGC5500.063.152495
AGGCGCG4200.062.583545
GCCTTCA30850.062.228534
ACCTTCA40350.061.187634
ATGCAGC950.059.2911032
GCGTAGT9800.058.911553
TGTGGCA40050.058.4816328
GGGCCCG5950.057.5873768
GGCCCGA6050.056.6355259
Produced by FastQC (version 0.11.5)