FastQCFastQC Report
Tue 7 Mar 2017
1HL_7A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_7A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2793544
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA140370.5024800038946943No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG75210.2692279054849324No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG69700.24950385603376932No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT60140.2152820932836569No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA57420.2055453574384366No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA55350.1981354150856403No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA53880.19287328211046614No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG48560.17382937229555004No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG47350.1694979567173454No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG46010.164701182440656No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC43200.15464227518879245No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA42850.15338938638517954No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA40720.14576466309462105No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA37760.13516880349835192No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG37050.13262722906816574No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC35650.1276156738537141No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA35020.1253604740072109No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA34740.12435816296432059No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT34270.12267571228518326No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC34070.1219597758259759No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT33740.12077848066828373No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA30360.10867915450767913No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA28990.10377498976210864No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA28550.10219992955185242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT550.086.999883
AGCTGCC2450.086.999882
GCAGCTC302.2702807E-886.999883
GCAGCTA356.6393113E-1086.999883
GCAGCCG356.6393113E-1086.999883
AGCAGCG950.086.999882
AGCAGCC1100.086.999882
GCTGCCG202.699223E-586.999873
GCTGCCA650.086.999873
TGCTGCA1450.086.999872
GCAGCCC202.699223E-586.999873
TGCAGCG257.8036646E-786.999872
CAGCAGC5250.085.342741
CAGCTGC6200.084.895041
CTGCTGC3150.084.2379761
AGCTGCA1300.083.6537252
CTGCAGC2050.082.755981
GCTGCAG1000.082.649883
TGCAGCT800.081.562382
AGCAGCA2350.081.4466862
Produced by FastQC (version 0.11.5)