##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_7A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2793544 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 24.608910044015772 32.0 12.0 32.0 2.0 32.0 2 13.76374705392147 12.0 12.0 12.0 12.0 22.0 3 14.251455498821569 12.0 12.0 12.0 12.0 22.0 4 19.729258962808533 22.0 12.0 22.0 12.0 22.0 5 23.875340069818122 22.0 22.0 27.0 22.0 32.0 6 31.680665491576292 32.0 27.0 37.0 22.0 37.0 7 34.97648972058432 37.0 32.0 41.0 22.0 41.0 8 36.18428455037758 41.0 37.0 41.0 27.0 41.0 9 36.729045613743686 41.0 37.0 41.0 27.0 41.0 10-11 37.31122921278491 41.0 37.0 41.0 27.0 41.0 12-13 38.32979756180679 41.0 37.0 41.0 32.0 41.0 14-15 37.91465249876143 41.0 37.0 41.0 32.0 41.0 16-17 37.458163716053875 41.0 37.0 41.0 27.0 41.0 18-19 37.56234535772481 41.0 37.0 41.0 29.5 41.0 20-21 35.98267612752833 41.0 37.0 41.0 24.5 41.0 22-23 37.283824060047024 41.0 37.0 41.0 27.0 41.0 24-25 36.73564493704055 41.0 37.0 41.0 27.0 41.0 26-27 36.53242028763463 41.0 37.0 41.0 24.5 41.0 28-29 36.95791260134081 41.0 37.0 41.0 27.0 41.0 30-31 37.01922199900915 41.0 37.0 41.0 27.0 41.0 32-33 37.34160317503501 41.0 37.0 41.0 27.0 41.0 34-35 37.70609698648026 41.0 37.0 41.0 29.5 41.0 36-37 37.51797984925241 41.0 37.0 41.0 27.0 41.0 38-39 37.0330921940016 41.0 37.0 41.0 27.0 41.0 40-41 37.00394749465195 41.0 37.0 41.0 27.0 41.0 42-43 36.134452151102685 41.0 37.0 41.0 24.5 41.0 44-45 37.06044418845738 41.0 37.0 41.0 27.0 41.0 46-47 36.80522250589216 41.0 37.0 41.0 27.0 41.0 48-49 36.988151251600115 41.0 37.0 41.0 27.0 41.0 50-51 37.5497634903907 41.0 37.0 41.0 27.0 41.0 52-53 37.223040338723855 41.0 37.0 41.0 27.0 41.0 54-55 37.00002827949014 41.0 37.0 41.0 27.0 41.0 56-57 36.3847811239057 41.0 37.0 41.0 24.5 41.0 58-59 35.13575622936313 41.0 32.0 41.0 22.0 41.0 60-61 35.35808904388118 41.0 32.0 41.0 22.0 41.0 62-63 35.03698563545088 39.0 32.0 41.0 22.0 41.0 64-65 34.750155000243424 37.0 32.0 41.0 22.0 41.0 66-67 35.716919976918206 41.0 32.0 41.0 22.0 41.0 68-69 36.52038808051708 41.0 37.0 41.0 24.5 41.0 70-71 36.92573895381637 41.0 37.0 41.0 27.0 41.0 72-73 37.22836314731395 41.0 37.0 41.0 27.0 41.0 74-75 37.0409035977239 41.0 37.0 41.0 27.0 41.0 76-77 35.93752255199847 39.0 34.5 41.0 24.5 41.0 78-79 36.62438984315264 41.0 37.0 41.0 27.0 41.0 80-81 37.41840758548997 41.0 37.0 41.0 27.0 41.0 82-83 37.61878871426403 41.0 37.0 41.0 27.0 41.0 84-85 37.57772080912275 41.0 37.0 41.0 27.0 41.0 86-87 37.47756720495543 41.0 37.0 41.0 27.0 41.0 88-89 37.3972275002649 41.0 37.0 41.0 27.0 41.0 90-91 37.21513192561134 41.0 37.0 41.0 27.0 41.0 92-93 37.09362444264347 41.0 37.0 41.0 27.0 41.0 94-95 36.92746149693723 41.0 37.0 41.0 27.0 41.0 96-97 36.81612407035651 41.0 37.0 41.0 27.0 41.0 98-99 36.66962056083599 41.0 37.0 41.0 24.5 41.0 100 36.51983394569765 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 2.751369539856263 1101 2 -0.7689692069020904 1101 3 -0.61637958024032 1101 4 -0.3767989377609453 1101 5 -1.5410738607992407 1101 6 -1.3829953952353193 1101 7 -1.0507297215868476 1101 8 -1.026552761212244 1101 9 -0.6339831621950509 1101 10-11 -0.2536586838170436 1101 12-13 -0.2368343800314392 1101 14-15 -0.16253637004132315 1101 16-17 -0.29652364785383156 1101 18-19 -0.49393906039124147 1101 20-21 -0.35931318638065335 1101 22-23 -0.24282934699164116 1101 24-25 -0.14616943174173258 1101 26-27 -0.25995329528203115 1101 28-29 -0.21954648588998538 1101 30-31 -0.38296203476614465 1101 32-33 -0.6104882118217176 1101 34-35 -0.34120726054211303 1101 36-37 0.015016259244305274 1101 38-39 -0.2711471490317692 1101 40-41 -0.3048397862406631 1101 42-43 -0.2601098444095129 1101 44-45 -0.08712683570502833 1101 46-47 0.10139663606874194 1101 48-49 -0.04781334895358924 1101 50-51 -0.22159045768951557 1101 52-53 -0.14139461938084708 1101 54-55 -0.41780320444335217 1101 56-57 -0.4521572076829372 1101 58-59 -0.49521338260955616 1101 60-61 -0.38005472360416803 1101 62-63 -0.5476079480063945 1101 64-65 -0.24842527123883684 1101 66-67 -0.11475500405752825 1101 68-69 -0.07393651366244569 1101 70-71 -0.11138407596060063 1101 72-73 -0.0370023836983151 1101 74-75 0.01432267689565947 1101 76-77 -0.34585432421746276 1101 78-79 -0.13305223647343212 1101 80-81 -0.21485487381570323 1101 82-83 -0.26653894987894233 1101 84-85 -0.039906693935002124 1101 86-87 -0.14066179619918273 1101 88-89 -0.309002476214431 1101 90-91 -0.07534203791946226 1101 92-93 -0.010499342570803094 1101 94-95 0.012608854598489927 1101 96-97 -0.058007127990549634 1101 98-99 -0.32579758786150137 1101 100 -0.38991698168459976 1102 1 4.78721011013716 1102 2 1.015988203404964 1102 3 -0.3009437909207744 1102 4 0.401880257602528 1102 5 -0.2536629047900085 1102 6 -0.5093435973828591 1102 7 -0.5989014182024661 1102 8 -1.1543880666449908 1102 9 -0.9258038445286871 1102 10-11 -0.7033471858479388 1102 12-13 -0.6288882698456888 1102 14-15 -0.62603361697834 1102 16-17 -1.1242977186430778 1102 18-19 -1.11722320078497 1102 20-21 -1.0499349676099072 1102 22-23 -0.7460222011749806 1102 24-25 -0.8497664678518646 1102 26-27 -0.9315388399125979 1102 28-29 -0.9817271047925189 1102 30-31 -0.9661582002793949 1102 32-33 -0.9781482497272904 1102 34-35 -0.5746759867783382 1102 36-37 -0.7228243376603558 1102 38-39 -0.9831375744627877 1102 40-41 -0.8441337907367483 1102 42-43 -0.806141739671645 1102 44-45 -0.36135395632895495 1102 46-47 -0.5498862130727247 1102 48-49 -0.7244495868834591 1102 50-51 -0.621274882957465 1102 52-53 -0.5869872226352655 1102 54-55 -0.7478777343260177 1102 56-57 -0.861141894030105 1102 58-59 -0.8962923936035097 1102 60-61 -0.8947438829090899 1102 62-63 -1.0263149319173337 1102 64-65 -0.8121149580269602 1102 66-67 -0.678554264249847 1102 68-69 -0.7467075643191379 1102 70-71 -0.5200197414796719 1102 72-73 -0.42791735906301653 1102 74-75 -0.5961151674452125 1102 76-77 -0.4821500775616627 1102 78-79 -0.6573743706338746 1102 80-81 -0.5168237839222414 1102 82-83 -0.5877261365297883 1102 84-85 -0.55311731947684 1102 86-87 -0.39647301378683153 1102 88-89 -0.631886178647342 1102 90-91 -0.6788237598775737 1102 92-93 -0.4362727211423376 1102 94-95 -0.42858175986120983 1102 96-97 -0.4603639961713313 1102 98-99 -0.45962298925626044 1102 100 -0.7894390852277198 1103 1 4.582392518775478 1103 2 1.1227407542386167 1103 3 0.8678846900187018 1103 4 3.929862969474126 1103 5 2.0855351964442015 1103 6 0.017238234209095538 1103 7 -1.2600963844752897 1103 8 -1.2630984289170755 1103 9 -1.0187596916558732 1103 10-11 -0.8497313068785175 1103 12-13 -0.9054615490549835 1103 14-15 -0.9795277100186865 1103 16-17 -0.9179223963037799 1103 18-19 -1.1024472492639887 1103 20-21 -1.0606894177566986 1103 22-23 -1.0753910490185135 1103 24-25 -1.0272008693663892 1103 26-27 -1.2796350111135197 1103 28-29 -0.8245746787552406 1103 30-31 -0.8363222555045269 1103 32-33 -1.2897007740143565 1103 34-35 -0.9914046867272219 1103 36-37 -0.8878860071812369 1103 38-39 -0.9268909483777463 1103 40-41 -1.0549867985413144 1103 42-43 -0.9796697548825719 1103 44-45 -1.0832332090012358 1103 46-47 -0.8741145657948692 1103 48-49 -0.8571656320427792 1103 50-51 -0.5925222855988466 1103 52-53 -0.5704754795296836 1103 54-55 -0.9035606899747322 1103 56-57 -1.2382214058737873 1103 58-59 -1.4909992555564244 1103 60-61 -1.4010199634000102 1103 62-63 -1.2401506686982557 1103 64-65 -1.319085320942193 1103 66-67 -1.3191591982220388 1103 68-69 -0.7528427015438268 1103 70-71 -0.817929675995785 1103 72-73 -0.7215218531006329 1103 74-75 -0.8481256615608288 1103 76-77 -0.9024735149900351 1103 78-79 -0.8861786585932805 1103 80-81 -0.6934236439368462 1103 82-83 -0.7900252222458732 1103 84-85 -0.6305208297851976 1103 86-87 -0.5822140024833189 1103 88-89 -0.675569696700741 1103 90-91 -0.869983025084224 1103 92-93 -0.5094085925035543 1103 94-95 -0.650354449845544 1103 96-97 -0.47898116484005726 1103 98-99 -0.6475602298197316 1103 100 -0.4733868103281438 1104 1 4.7925200010861175 1104 2 -0.8739439745005786 1104 3 -0.2787556722386615 1104 4 0.40418149040461415 1104 5 -0.6304878857432961 1104 6 -0.3899276786658632 1104 7 -0.6968465576190326 1104 8 -0.8967471167659866 1104 9 -0.8157697973734983 1104 10-11 -0.5789454307609461 1104 12-13 -0.28829867366961537 1104 14-15 -0.3166876880011529 1104 16-17 -0.31274675662862705 1104 18-19 -0.6999126538292373 1104 20-21 -0.756117192985208 1104 22-23 -0.31428336984165384 1104 24-25 -0.6300310818984087 1104 26-27 -0.515636506282739 1104 28-29 -0.3417901379030539 1104 30-31 -0.6651395581932675 1104 32-33 -0.633445168411022 1104 34-35 -0.8992924731831593 1104 36-37 -0.7199212892116265 1104 38-39 -0.71059187831424 1104 40-41 -0.5135379638405411 1104 42-43 -0.46591375379058064 1104 44-45 -0.15679435633177263 1104 46-47 -0.629274795933398 1104 48-49 -0.7036972391891183 1104 50-51 -0.42148326257030533 1104 52-53 -0.426789409033276 1104 54-55 -0.8057486562541598 1104 56-57 -0.9660557612136031 1104 58-59 -1.0334101612715827 1104 60-61 -0.9235932261797615 1104 62-63 -0.5939323603236986 1104 64-65 -0.8902935242824768 1104 66-67 -1.021707563154031 1104 68-69 -0.8828593855277163 1104 70-71 -0.5842335465912143 1104 72-73 -0.31850840033599326 1104 74-75 -0.2568539681962605 1104 76-77 -0.49767335891295517 1104 78-79 -0.322449974407121 1104 80-81 -0.36985062340458086 1104 82-83 -0.3890950465805716 1104 84-85 -0.4019923619475705 1104 86-87 -0.3132159401215091 1104 88-89 -0.22276840694643596 1104 90-91 -0.20223937075532206 1104 92-93 -0.36212135429911285 1104 94-95 -0.32311337874949686 1104 96-97 -0.4737096341127227 1104 98-99 -0.46131266040933383 1104 100 -0.41264351031608015 1105 1 3.2507008418182366 1105 2 0.608225959656032 1105 3 -0.37795892416198207 1105 4 -0.14871410678461316 1105 5 -0.4721456511500399 1105 6 0.35166376341359395 1105 7 -0.2249573124977431 1105 8 -0.44749773008067706 1105 9 -0.27668137702676177 1105 10-11 -0.101604535902176 1105 12-13 -0.08931878180825947 1105 14-15 -0.16746437855393737 1105 16-17 -0.006179420433241489 1105 18-19 -0.24307048287295885 1105 20-21 -0.492000963196908 1105 22-23 -0.09493694208747883 1105 24-25 -0.46517805547276225 1105 26-27 -0.1384282744587182 1105 28-29 -0.3222673448080826 1105 30-31 -0.48574372128018695 1105 32-33 -0.520001647826625 1105 34-35 -0.14831281588590173 1105 36-37 -0.33605377927173663 1105 38-39 -0.23844947759324953 1105 40-41 -0.11235742827107487 1105 42-43 -0.07786233773266815 1105 44-45 -0.1256763651832955 1105 46-47 -0.05090106554026619 1105 48-49 -0.12197613819895281 1105 50-51 -0.22722520176348837 1105 52-53 -0.32393239534551554 1105 54-55 -0.5135799850090095 1105 56-57 -0.3897263585215853 1105 58-59 -0.45538631140964725 1105 60-61 -0.47864879510336067 1105 62-63 -0.4835868221768109 1105 64-65 -0.57801319266256 1105 66-67 -0.4394024270355956 1105 68-69 -0.14346026602762407 1105 70-71 -0.27538314164873157 1105 72-73 -0.29200667001624225 1105 74-75 -0.26669959979204805 1105 76-77 -0.2805262972132567 1105 78-79 -0.1527524294185696 1105 80-81 -0.2199460624923617 1105 82-83 -0.17827859479177022 1105 84-85 -0.21612553702657067 1105 86-87 -0.23360182100061877 1105 88-89 -0.23972860361337212 1105 90-91 -0.04080202025512847 1105 92-93 -0.05479602552033924 1105 94-95 0.13195989714293432 1105 96-97 0.03919188287163422 1105 98-99 -0.2883371967643953 1105 100 -0.10934393706877188 1106 1 0.6113653936327808 1106 2 -0.33197351165764566 1106 3 -0.21915511101789953 1106 4 -0.9942203932479821 1106 5 -1.3144908773881987 1106 6 -1.2310306137453644 1106 7 -0.6460347332328524 1106 8 -0.5583441330024783 1106 9 -0.6848142361075844 1106 10-11 -0.407704300405193 1106 12-13 -0.3245977363586974 1106 14-15 -0.2696589315944493 1106 16-17 -0.5381566095175288 1106 18-19 -0.36714470088114837 1106 20-21 -0.7812076853176748 1106 22-23 -0.5401643821735718 1106 24-25 -0.5603446381382966 1106 26-27 -0.8282785868387776 1106 28-29 -0.7750591339040724 1106 30-31 -0.7002489412362891 1106 32-33 -0.7266515730268921 1106 34-35 -0.46380946291846925 1106 36-37 -0.7580385725287755 1106 38-39 -0.5901929485415494 1106 40-41 -0.6478597003081532 1106 42-43 -0.60817229791693 1106 44-45 -0.2949131995076826 1106 46-47 -0.7731179102652987 1106 48-49 -0.6735585513258755 1106 50-51 -0.6709199483137738 1106 52-53 -0.5065004903520034 1106 54-55 -0.739258691482334 1106 56-57 -0.9642682451737343 1106 58-59 -1.019492773033413 1106 60-61 -0.9434427572780066 1106 62-63 -0.8902542954680541 1106 64-65 -1.188605428714034 1106 66-67 -0.8820039034790952 1106 68-69 -0.9172828412059744 1106 70-71 -0.7778041371783644 1106 72-73 -0.6057618092431127 1106 74-75 -0.7395640011257001 1106 76-77 -0.5695064967166203 1106 78-79 -0.4036835599723574 1106 80-81 -0.5717463443836124 1106 82-83 -0.6521304167884168 1106 84-85 -0.7593199626631346 1106 86-87 -0.706741623195974 1106 88-89 -0.7168668865767387 1106 90-91 -0.7249129850876344 1106 92-93 -0.6474686158028575 1106 94-95 -0.5664502778652221 1106 96-97 -0.5689641332790174 1106 98-99 -0.7084667802015616 1106 100 -0.7820635466197388 1107 1 3.042743909977794 1107 2 -0.31388247899879573 1107 3 -0.28036717903440866 1107 4 -0.5848728401803243 1107 5 -1.3621564090245393 1107 6 -0.757744294893989 1107 7 -1.208318859405793 1107 8 -0.9133582135581833 1107 9 -0.8934712554266113 1107 10-11 -0.6792206445958229 1107 12-13 -0.4189147371224067 1107 14-15 -0.6196798083869197 1107 16-17 -0.8603490910831937 1107 18-19 -0.9312803401498613 1107 20-21 -1.1584701102904233 1107 22-23 -0.7561364819195191 1107 24-25 -0.9082505194875665 1107 26-27 -1.1445845512030317 1107 28-29 -0.6189994910817873 1107 30-31 -1.0051904657097097 1107 32-33 -0.8331624252675951 1107 34-35 -0.7177984068316832 1107 36-37 -0.6796490468544221 1107 38-39 -0.7288845765786292 1107 40-41 -0.9636013760460358 1107 42-43 -0.8893068962170929 1107 44-45 -0.47396823959194023 1107 46-47 -0.6875499671748599 1107 48-49 -0.7858109726646703 1107 50-51 -0.5746850716837173 1107 52-53 -0.46101992627665567 1107 54-55 -0.6474976827682255 1107 56-57 -0.9275184459436829 1107 58-59 -1.3165437722893856 1107 60-61 -0.7602243351697737 1107 62-63 -0.8918860485362003 1107 64-65 -0.832672933350814 1107 66-67 -0.8331709941073413 1107 68-69 -0.9998434597824613 1107 70-71 -0.6387575626539643 1107 72-73 -0.3628557761372022 1107 74-75 -0.5196814197533755 1107 76-77 -0.48084862427915454 1107 78-79 -0.999746828547984 1107 80-81 -0.7808697077702291 1107 82-83 -0.5430007370441601 1107 84-85 -0.5578955829991727 1107 86-87 -0.47934208404399214 1107 88-89 -0.20675554641790939 1107 90-91 -0.374796952777416 1107 92-93 -0.5399679821163303 1107 94-95 -0.45542831580765863 1107 96-97 -0.5331411159795749 1107 98-99 -0.6937780494859354 1107 100 -0.6190488557562261 1108 1 4.571178309683152 1108 2 -0.8372427404952738 1108 3 0.03047673148683394 1108 4 -0.3327973045483539 1108 5 -0.4185607795206785 1108 6 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0.9073140818576135 2224 26-27 0.9152211922721634 2224 28-29 0.6182708967642938 2224 30-31 0.6725411277195121 2224 32-33 0.6984671148280555 2224 34-35 0.5928341757966606 2224 36-37 0.5718639641740211 2224 38-39 0.7471934856687241 2224 40-41 0.5802500335365366 2224 42-43 0.799059257846352 2224 44-45 0.6844765704475648 2224 46-47 0.8389613600965049 2224 48-49 0.6722926558808666 2224 50-51 0.40716345491441075 2224 52-53 0.6256497526259679 2224 54-55 0.7556029967503619 2224 56-57 1.1875464064938157 2224 58-59 1.3790676865438982 2224 60-61 1.2347419668027442 2224 62-63 1.069854740495245 2224 64-65 1.0885059025747452 2224 66-67 0.6790661599704961 2224 68-69 0.5753271391381034 2224 70-71 0.5704715675857912 2224 72-73 0.5033845185562527 2224 74-75 0.37073983338036953 2224 76-77 0.6685562901990565 2224 78-79 0.40037719732498545 2224 80-81 0.22708702523101465 2224 82-83 0.2878276943219831 2224 84-85 0.2638605037009043 2224 86-87 0.3791145940308098 2224 88-89 0.18258769350995152 2224 90-91 0.2864457547562722 2224 92-93 0.2622403388476542 2224 94-95 0.5124577778628421 2224 96-97 0.3255036361256032 2224 98-99 0.34299733012772293 2224 100 0.7156232006767667 2225 1 3.4729899845668193 2225 2 1.3508807525139481 2225 3 0.9946988004356303 2225 4 3.8133853470408035 2225 5 2.4576421811519253 2225 6 1.4290211393266823 2225 7 1.0105339684957784 2225 8 1.0961446393330405 2225 9 0.7450641473364996 2225 10-11 0.6625841059260225 2225 12-13 0.3741245701327358 2225 14-15 0.4689266056995578 2225 16-17 0.3853189920407587 2225 18-19 0.542982578144148 2225 20-21 0.9452086833312237 2225 22-23 0.5266627211253763 2225 24-25 0.7403955162035416 2225 26-27 0.7509643504960408 2225 28-29 0.5712548832383462 2225 30-31 0.5808694913668759 2225 32-33 0.6593604668381587 2225 34-35 0.4721105222768571 2225 36-37 0.4207285117581847 2225 38-39 0.7173712722441437 2225 40-41 0.6029858740291587 2225 42-43 1.0406966317340078 2225 44-45 0.5042969809163083 2225 46-47 0.47667219876095857 2225 48-49 0.39571907258808636 2225 50-51 0.297281964588592 2225 52-53 0.48991554614087107 2225 54-55 0.5619433179034488 2225 56-57 0.8643782348423272 2225 58-59 1.330209947324434 2225 60-61 1.0817833856155161 2225 62-63 0.9224883168283569 2225 64-65 0.7051762150786871 2225 66-67 0.7352616457725318 2225 68-69 0.6163188531060939 2225 70-71 0.404262521790713 2225 72-73 0.3612115217914891 2225 74-75 0.1563989587324528 2225 76-77 0.41522381631670413 2225 78-79 0.37838507611591865 2225 80-81 0.21457618227982067 2225 82-83 0.14983175538544202 2225 84-85 0.131047117195088 2225 86-87 0.26251535959360695 2225 88-89 0.23374420142404517 2225 90-91 0.1859985775799231 2225 92-93 0.10402387193504836 2225 94-95 0.2406550930977005 2225 96-97 0.22769659148542587 2225 98-99 0.5342666740787436 2225 100 0.49361377487407765 2226 1 3.1316324470766865 2226 2 1.1022619679835621 2226 3 0.8842015628665703 2226 4 3.288523468587762 2226 5 2.10641881887884 2226 6 0.8840329783164229 2226 7 1.010139335504462 2226 8 1.257944165773445 2226 9 1.2671635948503095 2226 10-11 0.9484957081359582 2226 12-13 0.45384043437898924 2226 14-15 0.6826203704982987 2226 16-17 0.8247428278734361 2226 18-19 0.7110962324456551 2226 20-21 1.4158085254780204 2226 22-23 0.8964338339904145 2226 24-25 1.2808453978136427 2226 26-27 1.0197094175836483 2226 28-29 0.8934727206495481 2226 30-31 0.9687937218325473 2226 32-33 1.002691169284887 2226 34-35 0.7832786359311612 2226 36-37 0.7007206190983624 2226 38-39 0.9466530401999478 2226 40-41 0.9901208385121905 2226 42-43 1.4329618251041722 2226 44-45 0.7724500985169414 2226 46-47 0.7833020330151044 2226 48-49 0.643745907631498 2226 50-51 0.6905337404370471 2226 52-53 0.864619571397391 2226 54-55 0.968020665969739 2226 56-57 1.1810712103750873 2226 58-59 1.7021515416417188 2226 60-61 1.6421622332871806 2226 62-63 1.3571765567652179 2226 64-65 1.2499670595932812 2226 66-67 1.0864850080456208 2226 68-69 0.8667134860974102 2226 70-71 0.7440415273155736 2226 72-73 0.49637332131792533 2226 74-75 0.6258149019053008 2226 76-77 0.7402040846671412 2226 78-79 0.5660330634876658 2226 80-81 0.47799370398463026 2226 82-83 0.358000658305734 2226 84-85 0.3271797138801702 2226 86-87 0.4355619262865815 2226 88-89 0.4354016599875834 2226 90-91 0.4669772673983914 2226 92-93 0.4621533115562002 2226 94-95 0.670410420643087 2226 96-97 0.543008351548572 2226 98-99 0.7550638327212056 2226 100 0.5713564741637356 2227 1 2.771686034915348 2227 2 1.0588080514686062 2227 3 0.6586004000041434 2227 4 0.10798401556965231 2227 5 2.2366113270206185 2227 6 0.7389092660141579 2227 7 0.8281135633603327 2227 8 0.8240205964564424 2227 9 0.9805164252150647 2227 10-11 0.8159429941839846 2227 12-13 0.3651818227595385 2227 14-15 0.5890585032752966 2227 16-17 0.6273744526067233 2227 18-19 0.7867631147501157 2227 20-21 1.230215160373703 2227 22-23 0.7067294054015889 2227 24-25 0.8691142796973779 2227 26-27 0.8380540784918864 2227 28-29 0.6890691226089558 2227 30-31 0.6660837240943707 2227 32-33 0.8012041383959527 2227 34-35 0.642202561129622 2227 36-37 0.5814403853870544 2227 38-39 0.7575024422400176 2227 40-41 0.6986332856637958 2227 42-43 1.053951950580057 2227 44-45 0.6772310890694655 2227 46-47 0.757343011578385 2227 48-49 0.57193243063805 2227 50-51 0.4801822560950484 2227 52-53 0.43758768600070397 2227 54-55 0.7188575409825901 2227 56-57 1.130922135587788 2227 58-59 1.5488439003132797 2227 60-61 1.1355151585536518 2227 62-63 1.2911084299828488 2227 64-65 1.0873464750935824 2227 66-67 0.6416433911100867 2227 68-69 0.4197913070906196 2227 70-71 0.3432379469899658 2227 72-73 0.44193358153844287 2227 74-75 0.40813138965586404 2227 76-77 0.49862977582846923 2227 78-79 0.3947132336148158 2227 80-81 0.20059834499514295 2227 82-83 0.08334052509534473 2227 84-85 0.2543072918458833 2227 86-87 0.3277273146797768 2227 88-89 0.06369423566774657 2227 90-91 0.25624933956166274 2227 92-93 0.16195022187248753 2227 94-95 0.43775813896844085 2227 96-97 0.2582903757390227 2227 98-99 0.4121745023635697 2227 100 0.5558122048832672 2228 1 2.6558218483082427 2228 2 0.8485713489466171 2228 3 -0.3060815494157776 2228 4 -0.09242954026118255 2228 5 2.1141356676669076 2228 6 0.861455073619819 2228 7 0.9741043516194239 2228 8 0.8422333146533774 2228 9 0.9111195566968036 2228 10-11 0.768810861980036 2228 12-13 0.5591678855772955 2228 14-15 0.7019456140122742 2228 16-17 0.6907630436312502 2228 18-19 0.647465665436215 2228 20-21 1.3177259018247796 2228 22-23 0.7320288507521084 2228 24-25 0.9523324264124469 2228 26-27 0.9478747540514334 2228 28-29 0.6597534506923282 2228 30-31 0.7138702278895508 2228 32-33 0.7673352869300984 2228 34-35 0.5644048814424849 2228 36-37 0.7437046912461867 2228 38-39 0.8150044789307387 2228 40-41 0.7674169149749233 2228 42-43 1.1067772511414091 2228 44-45 0.6859914100296152 2228 46-47 0.6492184888655004 2228 48-49 0.5526509878798223 2228 50-51 0.4582189181223626 2228 52-53 0.7586449158143509 2228 54-55 0.49522993573231844 2228 56-57 1.237147553806885 2228 58-59 1.659854545310516 2228 60-61 1.4282454082336073 2228 62-63 1.500386891440364 2228 64-65 1.2782622764656182 2228 66-67 1.0808030150516856 2228 68-69 0.6972275638523513 2228 70-71 0.6830181438195879 2228 72-73 0.5209174841088995 2228 74-75 0.3935269221687747 2228 76-77 0.4011942524348271 2228 78-79 0.48792320168141856 2228 80-81 0.5094786354089109 2228 82-83 0.41224009033560804 2228 84-85 0.19364714896665447 2228 86-87 0.4176372060615847 2228 88-89 0.47258114233709847 2228 90-91 0.43772749077260187 2228 92-93 0.4300445648942528 2228 94-95 0.6464742187015062 2228 96-97 0.4072666895440378 2228 98-99 0.5595415514976381 2228 100 0.9807290234049546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 18.0 13 25.0 14 103.0 15 503.0 16 1921.0 17 4950.0 18 8629.0 19 11785.0 20 14286.0 21 16846.0 22 19673.0 23 22659.0 24 26141.0 25 29633.0 26 33840.0 27 38248.0 28 43599.0 29 49935.0 30 56856.0 31 66985.0 32 79733.0 33 97132.0 34 122224.0 35 161050.0 36 227673.0 37 357629.0 38 633730.0 39 663760.0 40 3978.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 2.6770769833227694 2.7687593610089505 92.51091024331558 2.0432534123526964 2 16.912599928473572 11.468608023346686 5.125523873825658 66.49326817435409 3 17.338835543667827 12.170919806525331 45.97486203904431 24.51538261076253 4 93.42602085379718 1.485961917907862 4.063440561523284 1.0245766667716707 5 17.405453431197074 35.15205058520646 26.42732672189878 21.015169261697686 6 17.86161234618105 28.21587918429064 30.358498022583504 23.564010446944813 7 20.681363887592248 26.874035275621218 35.387307305702 17.05729353108453 8 20.930724556334177 28.233884986239698 33.33475327397743 17.500637183448696 9 21.232813945296726 28.108918277285056 27.466293711500516 23.1919740659177 10-11 19.980641078143034 29.805687685606525 28.523570776046487 21.690100460203958 12-13 24.27169573845982 26.611734055379117 27.176715312162614 21.939854893998447 14-15 22.107527212744817 27.273742600796698 28.379578055688405 22.23915213077009 16-17 21.009065903382943 29.37358423565192 28.36500874874353 21.25234111222161 18-19 21.569930525525997 29.415072753462983 29.66749405056802 19.347502670442992 20-21 23.334284335596646 28.599692720071708 29.51838238452661 18.547640559805036 22-23 21.944275801634053 30.31962267284854 28.43375654723892 19.302344978278487 24-25 22.312285040078123 28.671984404040174 26.328348506413356 22.687382049468347 26-27 20.423376184516872 26.786655230774958 29.627687983436097 23.162280601272077 28-29 20.135856588822616 27.835458598320262 30.459625692760493 21.56905912009662 30-31 20.36225661800862 29.9940911516383 28.095634200579 21.54801802977408 32-33 21.12821562860653 30.027896464133015 28.444459797304066 20.399428109956386 34-35 21.465153224721 28.95528046094853 29.957054551494444 19.622511762836027 36-37 21.942468062074553 28.038667012225332 28.773897959008345 21.244966966691774 38-39 21.95604034485573 29.085031193664186 28.75949162274353 20.19943683873656 40-41 21.188652228387326 29.534974046894575 29.748505459101487 19.52786826561661 42-43 21.054164250377656 29.41885564552503 29.164142987034374 20.362837117062938 44-45 20.747838587829655 29.826915201622022 28.623658693043673 20.801587517504647 46-47 20.19941402932431 27.63351649167912 28.709341326218208 23.45772815277837 48-49 21.3628676267257 28.082970607044423 29.948182299649172 20.605979466580706 50-51 22.064034072847967 30.627457451896227 27.97423273089667 19.334275744359136 52-53 20.40870168635848 30.053039112698766 28.236843720853145 21.301415480089613 54-55 22.2527888108503 28.32128203755722 28.271568316623952 21.15436083496853 56-57 21.357480869735195 29.04245211079121 29.06046001422016 20.539607005253437 58-59 21.043565212846016 28.62934841214888 28.719175438734673 21.60791093627043 60-61 19.61926444582859 30.962202080514327 29.07656593426262 20.34196753939446 62-63 20.34340598115189 31.786739662350804 26.820740688763113 21.04911366773419 64-65 19.854929613513747 30.96402217902336 28.297030238151194 20.884017969311703 66-67 20.065533243793546 30.465673710526843 29.286920127264864 20.181872918414744 68-69 20.868831849435697 30.0709958389773 29.046007508741585 20.014164802845418 70-71 19.61515551571767 30.766241734513578 29.284861809944644 20.333740939824107 72-73 20.116758497449837 30.64199096201814 28.367335542235956 20.873914998296073 74-75 20.15813962479202 29.33213151466381 30.07491559109146 20.434813269452707 76-77 19.80344680448921 30.402510216413276 28.490798784626264 21.30324419447125 78-79 19.67622489568806 29.437946923334664 28.40844819340594 22.477379987571343 80-81 20.972054598176896 29.422597485302234 28.234121873832684 21.371226042688185 82-83 20.454054061793908 28.122503171598513 29.70305819417915 21.72038457242843 84-85 20.93502017508942 28.884868110185487 28.39010232163875 21.790009393086347 86-87 21.511599602512078 28.06742975947399 29.36332844587377 21.057642192140165 88-89 20.705723625616777 28.273225694673148 29.493843662387278 21.527207017322798 90-91 20.434401605988665 28.050641049505575 29.08409532837142 22.430862016134345 92-93 20.69970975935944 28.5255396013093 29.645801175854043 21.128949463477216 94-95 20.81769608783681 28.260320939995932 28.647374088254917 22.274608883912336 96-97 20.771267608457215 28.772680867027688 28.97140692969218 21.48464459482292 98-99 22.046225153425183 28.868276282743356 28.254396565795993 20.83110199803547 100 21.070188978587773 30.090773583662905 28.188136646496346 20.650900791252973 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 6.0 2 11.0 3 15.0 4 25.0 5 35.0 6 40.0 7 49.0 8 62.0 9 71.5 10 80.5 11 91.0 12 109.0 13 125.5 14 136.0 15 178.5 16 239.0 17 312.0 18 452.0 19 673.5 20 1065.5 21 1701.5 22 2654.0 23 4073.0 24 6242.0 25 9456.0 26 13674.5 27 18703.0 28 24241.0 29 30597.0 30 37911.0 31 46653.0 32 56737.5 33 67338.0 34 77936.0 35 89160.5 36 104010.5 37 116217.5 38 123559.0 39 130350.5 40 134068.5 41 135582.0 42 134865.5 43 131719.5 44 131273.0 45 129236.0 46 120512.0 47 110804.5 48 103254.0 49 100097.0 50 98618.5 51 91854.0 52 80239.5 53 67558.0 54 55944.0 55 45313.0 56 34776.0 57 25623.0 58 18552.0 59 13256.5 60 10131.5 61 8414.5 62 7240.0 63 5786.0 64 4785.5 65 4945.5 66 4246.0 67 2958.5 68 2316.0 69 2177.5 70 3705.0 71 4332.0 72 2267.0 73 729.5 74 307.5 75 154.5 76 105.5 77 81.0 78 72.5 79 70.5 80 60.0 81 43.5 82 28.0 83 20.5 84 21.0 85 17.5 86 13.5 87 16.0 88 17.5 89 12.0 90 9.0 91 11.5 92 13.0 93 12.0 94 10.0 95 8.0 96 11.5 97 15.5 98 27.5 99 79.5 100 96.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.330195622478113 2 0.006121256726223035 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.579682296036862E-4 30-31 0.009862024725581555 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0010023110428903214 40-41 0.0015750602102562194 42-43 1.4318729184147449E-4 44-45 0.0 46-47 0.002129910966141933 48-49 1.789841148018431E-5 50-51 0.0 52-53 0.0014139745069345607 54-55 0.019777744685603664 56-57 0.01166976428508017 58-59 0.014247135538226713 60-61 1.4318729184147449E-4 62-63 0.011437084935837774 64-65 1.2528888036129017E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.6847617220276466E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2793544.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.67307753792141 #Duplication Level Percentage of deduplicated Percentage of total 1 73.24405366132359 37.8474566403325 2 12.739343351010824 13.165621535179717 3 5.282679512537402 8.18916924178002 4 2.8083607841947638 5.804665782246151 5 1.6183711117809543 4.181310797209466 6 1.0407365665331003 3.2266836779409003 7 0.7281484731635876 2.63379707590308 8 0.5141048185035993 2.12523025193244 9 0.38854104014349444 1.8069400164599478 >10 1.5275774423336095 12.554180449675153 >50 0.06245937230461423 2.2233861671401565 >100 0.04096993074961904 4.114522337723408 >500 0.0034177338246739798 1.1845674693120265 >1k 0.0012362015961230775 0.9424685571649559 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCTGCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA 3521 0.1260406136434579 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.4318729184147449E-4 0.0 3 0.0 0.0 0.0 1.4318729184147449E-4 0.0 4 0.0 0.0 0.0 1.4318729184147449E-4 0.0 5 0.0 0.0 0.0 1.4318729184147449E-4 0.0 6 0.0 0.0 0.0 1.4318729184147449E-4 0.0 7 0.0 0.0 0.0 1.4318729184147449E-4 0.0 8 0.0 0.0 0.0 1.4318729184147449E-4 0.0 9 0.0 0.0 0.0 1.4318729184147449E-4 0.0 10-11 0.0 0.0 0.0 1.4318729184147449E-4 0.0 12-13 0.0 0.0 0.0 1.4318729184147449E-4 0.0 14-15 0.0 0.0 0.0 1.610857033216588E-4 0.0 16-17 0.0 0.0 0.0 1.7898411480184311E-4 0.0 18-19 0.0 0.0 0.0 1.7898411480184311E-4 0.0 20-21 0.0 0.0 0.0 1.7898411480184311E-4 0.0 22-23 0.0 0.0 0.0 1.7898411480184311E-4 0.0 24-25 0.0 0.0 0.0 1.7898411480184311E-4 0.0 26-27 0.0 0.0 0.0 1.7898411480184311E-4 0.0 28-29 0.0 0.0 0.0 1.7898411480184311E-4 0.0 30-31 0.0 0.0 0.0 1.7898411480184311E-4 0.0 32-33 0.0 0.0 0.0 1.7898411480184311E-4 0.0 34-35 0.0 0.0 0.0 1.7898411480184311E-4 0.0 36-37 0.0 0.0 0.0 1.7898411480184311E-4 0.0 38-39 0.0 0.0 0.0 1.7898411480184311E-4 0.0 40-41 0.0 0.0 0.0 1.7898411480184311E-4 0.0 42-43 0.0 0.0 0.0 1.7898411480184311E-4 0.0 44-45 0.0 0.0 0.0 1.7898411480184311E-4 0.0 46-47 0.0 0.0 0.0 1.7898411480184311E-4 0.0 48-49 0.0 0.0 0.0 1.7898411480184311E-4 0.0 50-51 3.579682296036862E-5 0.0 0.0 1.7898411480184311E-4 0.0 52-53 3.579682296036862E-5 0.0 0.0 1.7898411480184311E-4 0.0 54-55 3.579682296036862E-5 0.0 0.0 1.7898411480184311E-4 0.0 56-57 3.579682296036862E-5 0.0 1.0739046888110586E-4 1.7898411480184311E-4 0.0 58-59 3.579682296036862E-5 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 60-61 5.369523444055293E-5 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 62-63 8.949205740092154E-5 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 64-65 1.2528888036129017E-4 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 66-67 1.968825262820274E-4 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 68-69 3.042729951631333E-4 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 70-71 3.221714066433176E-4 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 72-73 3.937650525640548E-4 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 74-75 5.011555214451607E-4 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 76-77 6.264444018064508E-4 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 78-79 8.233269280884783E-4 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 80-81 0.0010202094543705058 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 82-83 0.0012170919806525331 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 84-85 0.0013602792724940077 0.0 2.1478093776221172E-4 1.7898411480184311E-4 0.0 86-87 0.0013602792724940077 7.159364592073724E-5 2.1478093776221172E-4 1.7898411480184311E-4 0.0 88 0.0013602792724940077 7.159364592073724E-5 2.1478093776221172E-4 1.7898411480184311E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGCCT 1805 0.0 88.531685 1 GCAGCCC 110 0.0 85.34296 4 GCCTTCA 11280 0.0 81.85131 4 TCACCGG 10240 0.0 81.04247 8 TCCGCCT 2380 0.0 76.70279 1 CACCGGC 10655 0.0 75.771416 9 CTGCTGC 215 0.0 74.232506 2 TGCCTTC 6200 0.0 73.966194 3 CTTCACC 11460 0.0 73.80751 6 CGGCCTT 1755 0.0 73.28703 2 TCTGCCT 6195 0.0 72.34543 1 CTGCAGC 280 0.0 72.08819 2 CCTTCAC 12555 0.0 71.29587 5 CTGCCTT 5090 0.0 71.01116 2 TCCGACT 1315 0.0 68.00329 1 TTCACCG 12695 0.0 67.36682 7 TCCGAAC 1195 0.0 67.30459 1 AGGCGCG 630 0.0 67.055176 5 GTGTGGC 4225 0.0 66.21401 7 TCTGAGG 3235 0.0 64.772385 1 >>END_MODULE