FastQCFastQC Report
Tue 7 Mar 2017
1HL_7A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_7A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2793544
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA35210.1260406136434579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCCT18050.088.5316851
GCAGCCC1100.085.342964
GCCTTCA112800.081.851314
TCACCGG102400.081.042478
TCCGCCT23800.076.702791
CACCGGC106550.075.7714169
CTGCTGC2150.074.2325062
TGCCTTC62000.073.9661943
CTTCACC114600.073.807516
CGGCCTT17550.073.287032
TCTGCCT61950.072.345431
CTGCAGC2800.072.088192
CCTTCAC125550.071.295875
CTGCCTT50900.071.011162
TCCGACT13150.068.003291
TTCACCG126950.067.366827
TCCGAAC11950.067.304591
AGGCGCG6300.067.0551765
GTGTGGC42250.066.214017
TCTGAGG32350.064.7723851
Produced by FastQC (version 0.11.5)