FastQCFastQC Report
Mon 6 Mar 2017
1HL_8A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_8A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2544013
Sequences flagged as poor quality0
Sequence length93
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA128280.5042427063069254No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG87500.34394478330102873No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG70490.27708191742730875No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG62630.24618584889306774No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA60040.2360050833073573No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA58950.23172051400680735No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC57590.2263746293749285No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA50020.19661849212248522No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT47450.18651634248724358No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA45830.18014845049927025No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG45080.17720035235669No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA44300.17413433028840655No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG43340.17036076466590383No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT39350.1546768825473769No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC34220.13451189125212804No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC33600.13207479678759504No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT33160.13034524587728127No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA33040.12987355017446844No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA32200.12657168025477858No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA31410.1234663502112607No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT28220.11092710611148605No Hit
CAGCCTTATTCAGGACACATCAGTACAAGTGACAGCCCTTCAACCGCTGA26460.10400890247023109No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA25570.10051049267436919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT302.2700988E-886.999593
GCTGCTA302.2700988E-886.999593
GCTGCAT600.086.999593
TGCTGCC302.2700988E-886.999592
TGCTGCA159.388603E-486.999592
AGCTGCC2350.086.999592
GCAGCCC302.2700988E-886.999593
GCAGCAA202.6992204E-586.999593
TGCAGCC159.388603E-486.999592
AGCAGCC550.086.999592
TGCTGCT257.80361E-786.999582
GCAGCGG257.80361E-786.999583
CAGCTGC5000.085.25961
GCTGCCC2000.084.824593
CAGCAGC3000.084.09961
AGCTGCA1150.083.2169952
CAGCGTA69400.082.486631
CAGCCCT145950.080.174341
GCGTAGT40750.079.527233
CGTAGTG37900.079.080094
Produced by FastQC (version 0.11.5)