##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_8A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2544013 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.777972046526491 12.0 12.0 12.0 2.0 27.0 2 14.655605533462289 12.0 12.0 12.0 12.0 22.0 3 13.708812022580073 12.0 12.0 12.0 12.0 22.0 4 13.746280384573506 12.0 12.0 12.0 12.0 22.0 5 22.443079496842195 22.0 22.0 22.0 22.0 27.0 6 26.741691964624394 27.0 22.0 32.0 22.0 32.0 7 34.945546661907784 37.0 37.0 37.0 27.0 41.0 8 37.559981808269065 41.0 37.0 41.0 27.0 41.0 9 38.5080618691807 41.0 37.0 41.0 32.0 41.0 10-11 38.87650318610793 41.0 41.0 41.0 37.0 41.0 12-13 39.055125701008606 41.0 41.0 41.0 37.0 41.0 14-15 39.016416189697146 41.0 41.0 41.0 37.0 41.0 16-17 38.9229025166145 41.0 41.0 41.0 37.0 41.0 18-19 38.86302900181721 41.0 41.0 41.0 37.0 41.0 20-21 38.87016202354312 41.0 41.0 41.0 37.0 41.0 22-23 38.85800190486448 41.0 41.0 41.0 37.0 41.0 24-25 38.883742339366975 41.0 41.0 41.0 37.0 41.0 26-27 38.84559689750013 41.0 41.0 41.0 37.0 41.0 28-29 38.79683181650408 41.0 41.0 41.0 34.5 41.0 30-31 38.67706257790349 41.0 41.0 41.0 32.0 41.0 32-33 38.72000850624585 41.0 41.0 41.0 32.0 41.0 34-35 38.69140763038554 41.0 41.0 41.0 32.0 41.0 36-37 38.61178166149308 41.0 41.0 41.0 32.0 41.0 38-39 38.51272202618461 41.0 41.0 41.0 32.0 41.0 40-41 38.55816381441447 41.0 41.0 41.0 32.0 41.0 42-43 38.30320599776809 41.0 41.0 41.0 32.0 41.0 44-45 38.4141238665054 41.0 41.0 41.0 32.0 41.0 46-47 38.426844713450755 41.0 41.0 41.0 32.0 41.0 48-49 38.36755472554582 41.0 41.0 41.0 32.0 41.0 50-51 38.26417769877749 41.0 41.0 41.0 32.0 41.0 52-53 38.23189248639846 41.0 41.0 41.0 32.0 41.0 54-55 38.08794333991217 41.0 37.0 41.0 32.0 41.0 56-57 37.86620095887875 41.0 37.0 41.0 27.0 41.0 58-59 37.8788492826098 41.0 37.0 41.0 29.5 41.0 60-61 37.87885537534596 41.0 37.0 41.0 29.5 41.0 62-63 37.8737131060258 41.0 37.0 41.0 29.5 41.0 64-65 37.855467916241 41.0 37.0 41.0 29.5 41.0 66-67 37.726550925643856 41.0 37.0 41.0 27.0 41.0 68-69 37.75430962813476 41.0 37.0 41.0 27.0 41.0 70-71 37.743352333498294 41.0 37.0 41.0 27.0 41.0 72-73 37.594982808657036 41.0 37.0 41.0 27.0 41.0 74-75 37.50473425253723 41.0 37.0 41.0 27.0 41.0 76-77 36.458887985242214 39.0 34.5 41.0 24.5 41.0 78-79 36.96830814150714 41.0 37.0 41.0 27.0 41.0 80-81 37.61329993203651 41.0 37.0 41.0 27.0 41.0 82-83 37.82964886578803 41.0 37.0 41.0 29.5 41.0 84-85 37.72349099631173 41.0 37.0 41.0 27.0 41.0 86-87 37.603512442743025 41.0 37.0 41.0 27.0 41.0 88-89 37.51194412135473 41.0 37.0 41.0 27.0 41.0 90-91 37.34522543713417 41.0 37.0 41.0 27.0 41.0 92-93 37.22860732236825 41.0 37.0 41.0 27.0 41.0 94-95 37.07913874653943 41.0 37.0 41.0 27.0 41.0 96-97 37.01484229836876 41.0 37.0 41.0 27.0 41.0 98-99 36.911870929904836 41.0 37.0 41.0 27.0 41.0 100 36.765073527533076 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.4540918220052763 1101 2 -0.6211253985413734 1101 3 -0.016272544480937512 1101 4 -0.087321614126882 1101 5 -1.0695343501782446 1101 6 -1.1011684305293983 1101 7 -0.5272695957162412 1101 8 -0.3561970394863039 1101 9 -0.3836488252778736 1101 10-11 -0.24818516232671328 1101 12-13 -0.1954440030028195 1101 14-15 -0.19659471278279028 1101 16-17 -0.16918065761018397 1101 18-19 -0.05006518157239981 1101 20-21 -0.1369349246050291 1101 22-23 0.0045071155581837274 1101 24-25 -0.2395270285045683 1101 26-27 -0.1577832809844324 1101 28-29 -0.11268616472854376 1101 30-31 -0.11688963681474718 1101 32-33 -0.29752094908860727 1101 34-35 -0.1623895984133057 1101 36-37 -0.15084047678094237 1101 38-39 -0.07740424411182545 1101 40-41 0.06532570288929662 1101 42-43 0.023127465774336997 1101 44-45 -0.15651991642010898 1101 46-47 -0.02241480635673554 1101 48-49 -0.035556916374012815 1101 50-51 0.12663309048717508 1101 52-53 -0.07339951221199925 1101 54-55 -0.03978056834836252 1101 56-57 0.020613823272810805 1101 58-59 -0.060780898198480315 1101 60-61 -0.06695623235115988 1101 62-63 -0.19986426713948902 1101 64-65 -0.11827068450400446 1101 66-67 -0.1227231988269537 1101 68-69 -0.00719058950185314 1101 70-71 -0.13922882855997187 1101 72-73 -0.1561073279896874 1101 74-75 0.06883716048646704 1101 76-77 -0.38101615603520855 1101 78-79 -0.1358643519652034 1101 80-81 -0.09406515145338545 1101 82-83 -0.11324224705153085 1101 84-85 -0.1858831779485044 1101 86-87 -0.1354033298599333 1101 88-89 -0.3228195468935553 1101 90-91 -0.12730940641721844 1101 92-93 0.0029513602678292727 1101 94-95 0.03698871563172901 1101 96-97 -0.08090339555293014 1101 98-99 -0.38328939800954487 1101 100 -0.35978834187706354 1102 1 1.6580842758460967 1102 2 0.8921719500628225 1102 3 -0.12324703090100186 1102 4 0.11342059552715966 1102 5 -0.7936820595487148 1102 6 -0.1078721453192415 1102 7 -0.1584429919604915 1102 8 -0.25497354556733853 1102 9 -0.23041157812934898 1102 10-11 -0.19719600894590883 1102 12-13 -0.22334923490148384 1102 14-15 -0.1358973626031954 1102 16-17 -0.1784958909267118 1102 18-19 0.12526604597817226 1102 20-21 0.025117169887373336 1102 22-23 -0.12026248240010062 1102 24-25 -0.0843099976582593 1102 26-27 -0.018565702687126873 1102 28-29 -0.21906810435927326 1102 30-31 -0.23377662953640765 1102 32-33 -0.22017232519927177 1102 34-35 -0.12250592190564902 1102 36-37 -0.056763876963692894 1102 38-39 -0.35029468695636723 1102 40-41 -0.25191703452327374 1102 42-43 -0.22210892278196326 1102 44-45 -0.10657702461848118 1102 46-47 -0.41227504881057797 1102 48-49 -0.4445714454873766 1102 50-51 -0.13741139089162147 1102 52-53 -0.14727733611394456 1102 54-55 -0.12881894505766667 1102 56-57 -0.12557085676374413 1102 58-59 -0.1873124810014275 1102 60-61 -0.1526264069053198 1102 62-63 -0.5231094700508265 1102 64-65 -0.31274487553682917 1102 66-67 -0.23830782923844396 1102 68-69 -0.4336986397885738 1102 70-71 -0.40910770010196984 1102 72-73 -0.20926462366049492 1102 74-75 -0.46731528295672575 1102 76-77 -0.26091052556069627 1102 78-79 -0.41317181856675234 1102 80-81 -0.3468871826147648 1102 82-83 -0.3580514072065455 1102 84-85 -0.3023504254121079 1102 86-87 -0.35419340406119915 1102 88-89 -0.29252256456953063 1102 90-91 -0.36773127846334575 1102 92-93 -0.24890219461905616 1102 94-95 -0.23221253759783878 1102 96-97 -0.21725847463793713 1102 98-99 -0.16479441565400776 1102 100 -0.6598287114655719 1103 1 1.4288653119026922 1103 2 0.884676799446666 1103 3 0.31815387051387845 1103 4 0.9581903924235551 1103 5 2.922657511423445 1103 6 -0.08665181213804729 1103 7 -0.4987684711937206 1103 8 -0.27579594401553464 1103 9 -0.20694769177607952 1103 10-11 -0.15185854888201078 1103 12-13 -0.2407570966084691 1103 14-15 -0.27198533613126585 1103 16-17 -0.25057128458257694 1103 18-19 -0.11710901107340277 1103 20-21 -0.13105652363156395 1103 22-23 -0.025713760355365878 1103 24-25 -0.24460424115438428 1103 26-27 -0.2175073959778544 1103 28-29 -0.12695263197223738 1103 30-31 -0.15463397207406615 1103 32-33 -0.4389187594758539 1103 34-35 -0.39746242008000365 1103 36-37 -0.29099125601435105 1103 38-39 -0.36631120338721956 1103 40-41 -0.553525888927652 1103 42-43 -0.2760939752694114 1103 44-45 -0.6189372482606572 1103 46-47 -0.5371773479890365 1103 48-49 -0.22415054749559005 1103 50-51 -0.3271920275871949 1103 52-53 -0.37198565993868016 1103 54-55 -0.28899694597049574 1103 56-57 -0.4325190254081974 1103 58-59 -0.6145824205267587 1103 60-61 -0.16768802260591542 1103 62-63 -0.27431468456475017 1103 64-65 -0.39396563660483963 1103 66-67 -0.36083749605725046 1103 68-69 -0.3599338063791677 1103 70-71 -0.3994039404032037 1103 72-73 -0.4422019239799866 1103 74-75 -0.4157477909439393 1103 76-77 -0.36698937083682637 1103 78-79 -0.43099972249190444 1103 80-81 -0.20451569128660196 1103 82-83 -0.23745935449089473 1103 84-85 -0.32932155731534607 1103 86-87 -0.32552997982569565 1103 88-89 -0.4329543023604927 1103 90-91 -0.5699099354692834 1103 92-93 -0.2720972254269114 1103 94-95 -0.43083117634726875 1103 96-97 -0.1940698621322312 1103 98-99 -0.4199361888169477 1103 100 -0.3448598045737441 1104 1 1.8416868835558482 1104 2 -0.5731412585539566 1104 3 0.18291084891759368 1104 4 0.0113443483616269 1104 5 -1.0329110844920244 1104 6 -0.5528693108521203 1104 7 0.028582234796878936 1104 8 -0.0037334662022630027 1104 9 0.0972882518026239 1104 10-11 -0.04619685928604156 1104 12-13 -0.04950428025296105 1104 14-15 -0.04333783879366848 1104 16-17 0.05677550930004571 1104 18-19 0.04792761060402739 1104 20-21 0.09765472657672092 1104 22-23 0.14412243823482385 1104 24-25 -0.027751749358941424 1104 26-27 -0.09400504509075347 1104 28-29 0.013074752783580834 1104 30-31 0.006781051869481303 1104 32-33 -0.04565772769359455 1104 34-35 -0.03777307043173295 1104 36-37 -0.20046073070518133 1104 38-39 -0.11213213026702107 1104 40-41 -0.10288075334413094 1104 42-43 8.644332271003918E-4 1104 44-45 -0.07521364593367963 1104 46-47 -0.20740330844776622 1104 48-49 -0.16969545342388415 1104 50-51 -0.04447630018866988 1104 52-53 -0.019106276023244106 1104 54-55 -0.07428379766538029 1104 56-57 -0.06114696220591043 1104 58-59 -0.08405638689712447 1104 60-61 -0.00205951348128508 1104 62-63 0.0064044188380592 1104 64-65 -0.05335216011733479 1104 66-67 -0.29009354352415784 1104 68-69 -0.22614478491329493 1104 70-71 0.002120332777842293 1104 72-73 0.017471487450613665 1104 74-75 -0.20599867297940477 1104 76-77 -0.308827192227362 1104 78-79 -0.07542025960984233 1104 80-81 -0.010205634995720914 1104 82-83 -0.10216634484827125 1104 84-85 -0.162795436749974 1104 86-87 0.021058154895712278 1104 88-89 -0.1089093276080817 1104 90-91 -0.14831872177401095 1104 92-93 -0.3772858397437773 1104 94-95 -0.17629204440056156 1104 96-97 -0.07588942701888612 1104 98-99 -0.17853449501908614 1104 100 -0.1416243463862088 1105 1 1.251598278363403 1105 2 0.42024581727490684 1105 3 -0.1088020183144689 1105 4 -0.15338439188630737 1105 5 -1.1215612288375745 1105 6 0.40372713095514356 1105 7 0.1598487795939434 1105 8 0.05673782951132722 1105 9 -0.0036317637806959624 1105 10-11 -0.15473022858089536 1105 12-13 -0.17366193156605902 1105 14-15 -0.12388115744271744 1105 16-17 -0.01245691200915644 1105 18-19 0.010299872284029732 1105 20-21 -0.0273165541528968 1105 22-23 0.04853471709643742 1105 24-25 -0.014597324593445649 1105 26-27 0.003433195991291882 1105 28-29 -0.09595608422082336 1105 30-31 -0.14492062733376798 1105 32-33 -0.23970210021499838 1105 34-35 -0.08905819534806625 1105 36-37 -0.21641715732869926 1105 38-39 -0.13436584019739684 1105 40-41 -0.05904415850061184 1105 42-43 0.07820967381967137 1105 44-45 0.09578688349982656 1105 46-47 -0.16853389871617708 1105 48-49 -0.30162678689706723 1105 50-51 -0.02872865017418036 1105 52-53 -0.10465324077096483 1105 54-55 -0.09269559704004848 1105 56-57 -0.05721516922440628 1105 58-59 -0.03917391114617175 1105 60-61 -0.13277115202804168 1105 62-63 -0.32411442599796203 1105 64-65 -0.14523484564382017 1105 66-67 -0.17064735095021177 1105 68-69 -0.3297614151189734 1105 70-71 -0.4300986928647106 1105 72-73 -0.25446404153337454 1105 74-75 -0.24004774047718058 1105 76-77 -0.211512319141562 1105 78-79 -0.1444464016378646 1105 80-81 -0.429536451022571 1105 82-83 -0.32276880022102716 1105 84-85 -0.17886474259272944 1105 86-87 -0.35363192671696453 1105 88-89 -0.2010312571434696 1105 90-91 -0.372975339193367 1105 92-93 -0.15550914081163114 1105 94-95 -0.07480713325549004 1105 96-97 -0.20944302466940456 1105 98-99 -0.4531341733632601 1105 100 -0.32287740561283584 1106 1 1.2868617483735694 1106 2 -0.735903099887226 1106 3 -0.15844620672517706 1106 4 -0.12435725162568723 1106 5 -1.194843398210054 1106 6 -0.5885851822822801 1106 7 -0.3195287811712859 1106 8 -0.3022044396732326 1106 9 -0.1330058588486267 1106 10-11 -0.16995137857627185 1106 12-13 -0.11366204708929928 1106 14-15 -0.2617527572085905 1106 16-17 -0.104128071246393 1106 18-19 -0.0842869010747691 1106 20-21 -0.24692797247026732 1106 22-23 -0.11433091396853001 1106 24-25 -0.26088498517075465 1106 26-27 -0.1939590593304814 1106 28-29 -0.3452731493143233 1106 30-31 -0.17257689177417035 1106 32-33 -0.16804476525921075 1106 34-35 -0.20271477555200335 1106 36-37 -0.4146138395011505 1106 38-39 -0.24135518895436547 1106 40-41 -0.2507744271306649 1106 42-43 -0.262381150554198 1106 44-45 -0.27598886278032353 1106 46-47 -0.2965985378215663 1106 48-49 -0.3864530014314269 1106 50-51 -0.2720548724101022 1106 52-53 -0.31973768826179594 1106 54-55 -0.2711266373653629 1106 56-57 -0.32562861150899636 1106 58-59 -0.43082677920322254 1106 60-61 -0.3387215617504751 1106 62-63 -0.36412782669418675 1106 64-65 -0.6486293036087645 1106 66-67 -0.4092244126550284 1106 68-69 -0.38072661181654865 1106 70-71 -0.7532682270749973 1106 72-73 -0.48210740587827416 1106 74-75 -0.606504219020664 1106 76-77 -0.4872591769093404 1106 78-79 -0.4223017511341851 1106 80-81 -0.43590909819918267 1106 82-83 -0.35204335276099386 1106 84-85 -0.5309343414960281 1106 86-87 -0.4993248351301318 1106 88-89 -0.7163003492848148 1106 90-91 -0.7235208014403725 1106 92-93 -0.4034897945191531 1106 94-95 -0.4122840286068339 1106 96-97 -0.5826493337787966 1106 98-99 -0.9168704646050614 1106 100 -0.772150764412622 1107 1 1.5423801246299575 1107 2 -0.6704763357143122 1107 3 -0.1900885634885192 1107 4 -0.4634434843546824 1107 5 -1.2328436458726415 1107 6 -0.2713286376830162 1107 7 -0.45797024511868756 1107 8 -0.2784476751003666 1107 9 -0.2370243381695687 1107 10-11 -0.24080047283881356 1107 12-13 -0.3611322545105651 1107 14-15 -0.4211351697806194 1107 16-17 -0.4003615616890812 1107 18-19 -0.2816721571517391 1107 20-21 -0.27984411868656167 1107 22-23 -0.16646283121538374 1107 24-25 -0.3232574043266041 1107 26-27 -0.37779084566996346 1107 28-29 -0.17957812887061664 1107 30-31 -0.5712498202535272 1107 32-33 -0.3742928039561022 1107 34-35 -0.3469806367255899 1107 36-37 -0.3640908637966973 1107 38-39 -0.36165661816747985 1107 40-41 -0.4307760000188381 1107 42-43 -0.3954500861280863 1107 44-45 -0.13387457034156824 1107 46-47 -0.3963174369382827 1107 48-49 -0.42841094724716555 1107 50-51 -0.44962837128253597 1107 52-53 -0.2592296607635589 1107 54-55 -0.3546658434298635 1107 56-57 -0.2813411375443309 1107 58-59 -0.45603135080848034 1107 60-61 -0.33413605431906035 1107 62-63 -0.3589472945118928 1107 64-65 -0.3335884546644792 1107 66-67 -0.517609098800385 1107 68-69 -0.438546308068382 1107 70-71 -0.42125662615941195 1107 72-73 -0.2801898090668189 1107 74-75 -0.3910561304835909 1107 76-77 -0.5189187431460098 1107 78-79 -0.6809424450201504 1107 80-81 -0.5474482135053478 1107 82-83 -0.47300931761444076 1107 84-85 -0.45497086095983974 1107 86-87 -0.22262630818413953 1107 88-89 -0.34265287928890586 1107 90-91 -0.3256478731148533 1107 92-93 -0.2717563717606595 1107 94-95 -0.3816444361271607 1107 96-97 -0.5840137854292138 1107 98-99 -0.3727725832738926 1107 100 -0.3731734493827332 1108 1 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0.2776940031864896 2223 26-27 0.19115560699312084 2223 28-29 0.110578037934701 2223 30-31 0.2669495257417367 2223 32-33 0.23613076518931564 2223 34-35 0.28233175643112673 2223 36-37 0.2126074663502422 2223 38-39 0.22156711377575533 2223 40-41 0.24854757970612695 2223 42-43 0.28434600807342036 2223 44-45 0.26022456789763027 2223 46-47 0.337266105528947 2223 48-49 0.2626387768078544 2223 50-51 0.26143548195739186 2223 52-53 0.34123978969169855 2223 54-55 0.23108031030808007 2223 56-57 0.2809074416844197 2223 58-59 0.2565305005078571 2223 60-61 0.08008744078881591 2223 62-63 0.1282470235978792 2223 64-65 0.12799324707751936 2223 66-67 0.15058601344974676 2223 68-69 0.313473392624914 2223 70-71 0.4488287752437472 2223 72-73 0.2695291392454564 2223 74-75 0.14268741556026043 2223 76-77 0.20956339659687018 2223 78-79 0.4170338749130025 2223 80-81 0.31478028578520423 2223 82-83 0.13453050647219555 2223 84-85 0.1786093862392235 2223 86-87 0.33640822834602346 2223 88-89 0.34017515068608617 2223 90-91 0.3496818801312287 2223 92-93 0.37143064203070963 2223 94-95 0.34996502873092794 2223 96-97 0.3444785099062031 2223 98-99 0.36298564347871576 2223 100 0.626429384085398 2224 1 1.1201201162193168 2224 2 -0.999753327329687 2224 3 -0.011117806969592081 2224 4 -0.20353777983534194 2224 5 -1.1095312588930604 2224 6 0.6462406714756135 2224 7 0.2862404495979476 2224 8 0.23348196130442744 2224 9 0.139352244213363 2224 10-11 0.19604215458959118 2224 12-13 0.10215156890635768 2224 14-15 0.021769545731189055 2224 16-17 0.12549970389109433 2224 18-19 0.1071827336594211 2224 20-21 0.10433696404952997 2224 22-23 0.2160483548209342 2224 24-25 0.16212192296965355 2224 26-27 0.18474961064032414 2224 28-29 0.1846104985911765 2224 30-31 0.17174383604169918 2224 32-33 0.31563751120219763 2224 34-35 0.2775982972974802 2224 36-37 0.15893579048051976 2224 38-39 0.15932756275993398 2224 40-41 0.20769398552464224 2224 42-43 0.38293378936025846 2224 44-45 0.3307945237360741 2224 46-47 0.3586076031248666 2224 48-49 0.1865640906997399 2224 50-51 0.3145811857065439 2224 52-53 0.2933915975009853 2224 54-55 0.36976887488937393 2224 56-57 0.2994711067339253 2224 58-59 0.37514910171197613 2224 60-61 0.23833942338089287 2224 62-63 0.42775383533310674 2224 64-65 0.2732722172005211 2224 66-67 0.30891666956372177 2224 68-69 0.3302962851893554 2224 70-71 0.5148274515182507 2224 72-73 0.43940160695354535 2224 74-75 0.3819687764304902 2224 76-77 0.6673401679205782 2224 78-79 0.43047593234737747 2224 80-81 0.2936253236896107 2224 82-83 0.2330544984169549 2224 84-85 0.17908712975117425 2224 86-87 0.35244293593375886 2224 88-89 0.41026968262502095 2224 90-91 0.3957358521002661 2224 92-93 0.3364620012578783 2224 94-95 0.6396331076549657 2224 96-97 0.4971761805127315 2224 98-99 0.46689413244837397 2224 100 0.6598074472785953 2225 1 1.6852073614599554 2225 2 1.6489703746171784 2225 3 0.8646036783633733 2225 4 0.9408473153363488 2225 5 3.192634787049329 2225 6 -0.24337459322283195 2225 7 0.290953662571269 2225 8 0.4667759209152109 2225 9 0.2692141750717596 2225 10-11 0.276387816060371 2225 12-13 0.20277036715726382 2225 14-15 0.20418705868728892 2225 16-17 0.21429416995704287 2225 18-19 0.1481272686746351 2225 20-21 0.1416244351396969 2225 22-23 0.10109233808697127 2225 24-25 0.1929295987514763 2225 26-27 0.13917814617052215 2225 28-29 0.1436547176341989 2225 30-31 0.1880791481437285 2225 32-33 0.19160311102970695 2225 34-35 0.16752553993215713 2225 36-37 0.32583913897907024 2225 38-39 0.32817199958643783 2225 40-41 0.23032624943354563 2225 42-43 0.42893143624389296 2225 44-45 0.1502504295617868 2225 46-47 0.2879669955027566 2225 48-49 0.29992822498714133 2225 50-51 0.09587146750657638 2225 52-53 0.07385976217879886 2225 54-55 0.26476629220068304 2225 56-57 0.33585308561637817 2225 58-59 0.3327356140274489 2225 60-61 0.3288260158915648 2225 62-63 0.36334154049399103 2225 64-65 0.33334357851789775 2225 66-67 0.41863731261442894 2225 68-69 0.4083597960637988 2225 70-71 0.5304443806160819 2225 72-73 0.6506548779714407 2225 74-75 0.257323023596129 2225 76-77 0.40713021006571637 2225 78-79 0.37360675600015725 2225 80-81 0.36460815695339477 2225 82-83 0.1640530837550358 2225 84-85 0.25471240411337703 2225 86-87 0.2975330740959592 2225 88-89 0.44558038860646576 2225 90-91 0.5765636154608274 2225 92-93 0.41151198753007634 2225 94-95 0.639875477089582 2225 96-97 0.5560232853419791 2225 98-99 0.6155493892669099 2225 100 0.7691059797219779 2226 1 1.4373743455325076 2226 2 1.4046392845575966 2226 3 0.8727786659631107 2226 4 1.074276431764094 2226 5 3.106579264493096 2226 6 -0.3508381731938073 2226 7 0.24624995229037694 2226 8 0.2604554614012997 2226 9 0.2302482650762272 2226 10-11 0.01598996666385233 2226 12-13 0.022894231997469205 2226 14-15 0.07579688826788811 2226 16-17 8.431770175434394E-4 2226 18-19 0.01466064528131028 2226 20-21 0.04144447824975117 2226 22-23 0.0362052876347434 2226 24-25 0.11502035948459621 2226 26-27 0.1755327293686264 2226 28-29 0.14425942177312834 2226 30-31 0.07703020844267883 2226 32-33 0.05282963473104019 2226 34-35 0.004263459627445343 2226 36-37 0.10693930247882122 2226 38-39 0.19868723813074496 2226 40-41 0.030008052584747702 2226 42-43 0.11793898662569546 2226 44-45 0.06413290569511076 2226 46-47 0.08624955920335964 2226 48-49 0.036542448240844294 2226 50-51 -0.006346495421233556 2226 52-53 0.07650859594180304 2226 54-55 0.16486572393187515 2226 56-57 0.3358021833059439 2226 58-59 0.2298532419950874 2226 60-61 0.40072725163126677 2226 62-63 0.29978904911293114 2226 64-65 0.32691649205899154 2226 66-67 0.3203158641413424 2226 68-69 0.36362278878285537 2226 70-71 0.4761220472847896 2226 72-73 0.32918267599107054 2226 74-75 0.4056703442209795 2226 76-77 0.42225585074592686 2226 78-79 0.33866289397680305 2226 80-81 0.28956207696711544 2226 82-83 0.14610255620808488 2226 84-85 0.08495358155653321 2226 86-87 0.005785907785487154 2226 88-89 0.08409437793918073 2226 90-91 0.15126371282584472 2226 92-93 0.3081263332555082 2226 94-95 0.43695666971922975 2226 96-97 0.31612567170352435 2226 98-99 0.40962849375365806 2226 100 0.5811011665832098 2227 1 1.4109682834314317 2227 2 1.8630355720602942 2227 3 0.6238887149902759 2227 4 -0.08883525150191751 2227 5 3.1785050453817547 2227 6 -0.02886614026235179 2227 7 0.3333040055110601 2227 8 0.43782433685996835 2227 9 0.4092020438681274 2227 10-11 0.33227222746370444 2227 12-13 0.2560873959455421 2227 14-15 0.2888892998493944 2227 16-17 0.39153610970539887 2227 18-19 0.28854828365200547 2227 20-21 0.3091069770934425 2227 22-23 0.3281651408615929 2227 24-25 0.385010877815823 2227 26-27 0.3047196955429712 2227 28-29 0.22789776163314457 2227 30-31 0.3330237023219951 2227 32-33 0.2429301558373993 2227 34-35 0.38676438466830376 2227 36-37 0.31936799724616804 2227 38-39 0.21287890659610298 2227 40-41 0.2468503485361495 2227 42-43 0.4584141492787168 2227 44-45 0.31948966311855287 2227 46-47 0.3089026534650259 2227 48-49 0.20638264743298151 2227 50-51 0.231603305694982 2227 52-53 0.2562644622425623 2227 54-55 0.1698536213140116 2227 56-57 0.39792985436142203 2227 58-59 0.5032552339163985 2227 60-61 0.4459197372160446 2227 62-63 0.4708341140199721 2227 64-65 0.4455140530345929 2227 66-67 0.43376865622299476 2227 68-69 0.43339206817602616 2227 70-71 0.6546387442722406 2227 72-73 0.46201777204114336 2227 74-75 0.6176839269042063 2227 76-77 0.6446599548438599 2227 78-79 0.3965646700552412 2227 80-81 0.4583889709882669 2227 82-83 0.4051024328692989 2227 84-85 0.4896296630834698 2227 86-87 0.5118167887327232 2227 88-89 0.3945527356833125 2227 90-91 0.3180639965050389 2227 92-93 0.40544949627854265 2227 94-95 0.5954601077877442 2227 96-97 0.4487418414176858 2227 98-99 0.6382199648769671 2227 100 0.5569498915685642 2228 1 1.3030916038312874 2228 2 1.52902689452441 2228 3 -0.26710212963596014 2228 4 -0.21881193301341284 2228 5 3.2589251604451164 2228 6 0.13028158809636992 2228 7 0.36825497934466256 2228 8 0.2808631946363036 2228 9 0.18664045056549838 2228 10-11 0.21975574205687565 2228 12-13 0.2091455583721995 2228 14-15 0.2228248681897682 2228 16-17 0.17453702715413044 2228 18-19 0.028750695777617352 2228 20-21 0.2588919500478255 2228 22-23 0.23370041485383553 2228 24-25 0.17427290499524162 2228 26-27 0.21581604677292177 2228 28-29 0.13035337049416285 2228 30-31 0.22648592445926852 2228 32-33 0.21348578572328591 2228 34-35 0.2630516799147742 2228 36-37 0.27842732559724936 2228 38-39 0.13864535855427818 2228 40-41 0.23417522095343202 2228 42-43 0.3629053475049844 2228 44-45 0.3783755363195027 2228 46-47 0.2982999087642426 2228 48-49 0.14385660526856014 2228 50-51 0.16519100805932396 2228 52-53 0.2241661805475772 2228 54-55 0.09612705926198117 2228 56-57 0.3369209474633479 2228 58-59 0.2913857409979599 2228 60-61 0.11753712286944307 2228 62-63 0.24796909366407505 2228 64-65 0.2668963424514388 2228 66-67 0.2966698011225546 2228 68-69 0.2844844055677953 2228 70-71 0.21092517941052336 2228 72-73 0.34807589624076485 2228 74-75 0.3298740490395744 2228 76-77 0.3673118595056408 2228 78-79 0.32949200047058014 2228 80-81 0.3937026237721568 2228 82-83 0.3658055235247062 2228 84-85 0.3991749525576509 2228 86-87 0.40625427798137537 2228 88-89 0.4369431674576205 2228 90-91 0.40915423141014884 2228 92-93 0.37820604743586017 2228 94-95 0.7165026544725066 2228 96-97 0.535269680252533 2228 98-99 0.5995346880176982 2228 100 0.6992203860352006 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 22.0 13 48.0 14 135.0 15 473.0 16 1717.0 17 4210.0 18 6914.0 19 8957.0 20 10510.0 21 12030.0 22 13755.0 23 15804.0 24 17950.0 25 20243.0 26 22887.0 27 25599.0 28 29055.0 29 33142.0 30 37729.0 31 43624.0 32 51275.0 33 61254.0 34 74826.0 35 97078.0 36 136761.0 37 226730.0 38 554776.0 39 1035214.0 40 1295.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.13139989982669 19.033903789299185 19.145601460934625 36.689094849939494 2 19.070907624990223 53.02428318550675 11.001395910701026 16.90341327880201 3 40.86343112240385 15.624409151997257 23.57311067199735 19.939049053601536 4 17.77946889422342 16.4868654366153 21.71663430965172 44.01703135950956 5 16.511000533409224 33.6152763370313 27.028085155225227 22.845637974334252 6 17.296373878592604 28.400208646732544 28.56655999792454 25.736857476750313 7 19.871714492025003 25.909851875756924 36.39163793581244 17.82679569640564 8 22.08019377259472 28.152057399077755 33.01213476503461 16.755614063292914 9 21.036409798220372 27.975328742423876 26.69369220990616 24.29456924944959 10-11 19.733055609385644 30.30994731552079 28.155575462861236 21.80142161223233 12-13 24.495885044612585 26.496739599994186 27.086654038324486 21.920721317068743 14-15 21.95155842364013 27.545830151025168 28.40474871787212 22.09786270746258 16-17 20.809701051055953 30.217553919732325 28.161845084911125 20.810899944300598 18-19 21.570172793928332 29.6526393536511 29.52300165132804 19.254186201092526 20-21 22.649491177914577 28.98988723721144 30.06346665681347 18.297154928060507 22-23 21.759067268917256 30.89852528269313 28.394096256583595 18.948311191806017 24-25 22.480604462319963 28.1787278602743 26.31704319121011 23.02362448619563 26-27 19.99062111710907 26.581310708711005 30.237306177287614 23.190761996892313 28-29 19.446907207478294 28.293455571110655 30.479203350152623 21.78043387125843 30-31 19.923523020144888 30.35167853093752 28.339902318289532 21.384896130628057 32-33 20.681046048113746 30.270049720657877 28.684778733442005 20.36412549778637 34-35 21.305040500972282 29.085051845253933 30.271228959914907 19.338678693858878 36-37 21.63797905120768 28.02772627341134 28.794703486185014 21.539591189195967 38-39 21.686315544521296 29.119011979578584 29.082022927709005 20.112649548191115 40-41 20.966719595656325 30.008201733154166 29.896526085461943 19.12855258572757 42-43 20.72325608910975 30.0483606779693 29.282600808409086 19.94578242451186 44-45 20.470512532758285 30.4186142130563 28.68967257635869 20.421200677826725 46-47 19.63734935662402 28.180416110529887 28.903677273671573 23.278557259174512 48-49 20.75373873113388 28.17420440119866 30.33875829570739 20.73329857196007 50-51 21.746724564693658 31.275665651079613 27.873815896381032 19.1037938878457 52-53 20.318273130059865 30.66725996308538 28.062776473936395 20.95169043291836 54-55 22.367094935376368 28.789336732276787 27.944272843842754 20.89929548850409 56-57 21.159040325445073 28.834286367997375 29.578669635550973 20.428003671006575 58-59 21.415328293828207 28.063311731710037 28.623513741746066 21.897846232715686 60-61 19.356527054263793 30.645762640243788 29.910721568234372 20.086988737258046 62-63 20.521246525743894 31.506284128300788 27.176545282284536 20.79592406367078 64-65 19.701774205467583 30.52200618550541 28.703559574404526 21.07266003462248 66-67 20.197243488928713 30.72928479532141 29.314138724920035 19.759332990829844 68-69 20.78413121316597 30.047979314571112 29.373081033784025 19.794808438478892 70-71 19.353202990707988 31.173091489705442 29.39973184099295 20.073973678593624 72-73 19.953003384809747 30.933686266540306 28.796767154884822 20.316543193765128 74-75 19.779340750224154 29.387939448422628 30.440469447286628 20.392250354066586 76-77 19.22101026999469 30.920203631034905 28.654393668585815 21.204392430384594 78-79 19.154579791848548 29.676007944927957 28.582283188018298 22.587129075205198 80-81 20.973337942856716 29.63575003778486 28.05680484165049 21.334107177707935 82-83 20.07709866262476 28.23710020349739 30.123037893281207 21.562763240596645 84-85 20.364479269563482 29.13461920202452 28.42910000852983 22.071801519882168 86-87 20.972612954414934 28.34555090716911 29.697175289591684 20.98466084882428 88-89 21.19843727213658 27.70705181144908 29.42095814761953 21.673552768794814 90-91 20.81251157128521 28.40268504917231 29.379429271784378 21.405374107758096 92-93 21.529430077597876 28.778685486276995 29.306159205947452 20.385725230177677 94-95 21.342245499531646 28.77827275253704 29.523473347030855 20.356008400900468 96-97 20.948438549645775 28.862784899291 29.637604053123944 20.55117249793928 98-99 22.3417097318292 28.915241392241313 28.534406074182794 20.20864280174669 100 21.412154733486034 30.62728059958813 28.09317405217662 19.867390614749215 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 5.0 2 12.0 3 20.0 4 23.5 5 25.5 6 25.0 7 32.0 8 46.0 9 51.0 10 69.5 11 99.0 12 114.0 13 131.5 14 151.0 15 182.5 16 227.0 17 281.5 18 414.5 19 706.5 20 1122.0 21 1740.5 22 2686.0 23 4111.5 24 6421.0 25 9548.0 26 13595.0 27 18596.0 28 23933.5 29 29840.5 30 36473.0 31 44389.5 32 53813.5 33 63667.0 34 74388.5 35 87596.0 36 101669.5 37 111451.0 38 116902.5 39 121385.5 40 124758.5 41 125203.0 42 122709.5 43 120070.5 44 118826.5 45 115713.0 46 108337.0 47 99393.5 48 91647.0 49 87433.0 50 84826.5 51 78452.0 52 68012.5 53 56980.0 54 47189.0 55 38495.5 56 30345.0 57 22744.0 58 16053.0 59 11092.5 60 8249.5 61 6773.5 62 5778.5 63 4476.0 64 3308.5 65 2953.5 66 2976.5 67 2538.5 68 1805.0 69 1873.5 70 2840.0 71 2879.5 72 1588.5 73 565.0 74 220.0 75 116.0 76 99.0 77 76.0 78 54.5 79 47.5 80 42.0 81 29.0 82 26.5 83 29.5 84 24.0 85 19.5 86 17.0 87 19.0 88 19.0 89 12.5 90 12.5 91 13.5 92 11.5 93 9.0 94 7.5 95 7.5 96 10.5 97 12.5 98 36.0 99 88.5 100 88.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.317670153415097 2 0.006053428186098105 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.7342576472683116E-4 30-31 0.009473222031491191 32-33 0.0 34-35 0.0 36-37 0.0 38-39 6.682355789848558E-4 40-41 0.0015133570465245263 42-43 1.5723190093761314E-4 44-45 0.0 46-47 0.0019457447741029626 48-49 5.896196285160493E-5 50-51 0.0 52-53 0.0013561251455869133 54-55 0.01876955817442757 56-57 0.011654814657000573 58-59 0.014819106663370036 60-61 2.1619386378921805E-4 62-63 0.011910316496024195 64-65 3.9307975234403285E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.7688588855481477E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2544013.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.50312719161465 #Duplication Level Percentage of deduplicated Percentage of total 1 85.90555055469092 63.1432660888713 2 8.661081816242556 12.732331967125148 3 2.4566284548106485 5.417096213294607 4 1.0715411193248174 3.1504649273910874 5 0.5532050227690034 2.0331149575815073 6 0.33801941984440537 1.4907290646035474 7 0.21913861292270723 1.1275161336776232 8 0.15732005145675074 0.9250812601613531 9 0.11040417981208306 0.7303547224092092 >10 0.49080121420148554 6.1437954627687255 >50 0.024904907912038646 1.261191987814056 >100 0.010588357855772057 1.418459118207363 >500 7.074497359915665E-4 0.33103852257966865 >1k 1.0883842091601659E-4 0.09555957351481768 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 3.9307975234403285E-5 0.0 0.0 0.0 0.0 12-13 3.9307975234403285E-5 0.0 0.0 0.0 0.0 14-15 3.9307975234403285E-5 0.0 0.0 0.0 0.0 16-17 3.9307975234403285E-5 0.0 0.0 0.0 0.0 18-19 3.9307975234403285E-5 0.0 0.0 0.0 0.0 20-21 3.9307975234403285E-5 0.0 0.0 0.0 0.0 22-23 3.9307975234403285E-5 0.0 0.0 0.0 0.0 24-25 3.9307975234403285E-5 0.0 0.0 0.0 0.0 26-27 3.9307975234403285E-5 0.0 0.0 0.0 0.0 28-29 3.9307975234403285E-5 0.0 0.0 0.0 0.0 30-31 3.9307975234403285E-5 0.0 0.0 0.0 0.0 32-33 3.9307975234403285E-5 0.0 0.0 0.0 0.0 34-35 3.9307975234403285E-5 0.0 0.0 0.0 0.0 36-37 3.9307975234403285E-5 0.0 0.0 0.0 0.0 38-39 5.896196285160493E-5 0.0 0.0 0.0 0.0 40-41 7.861595046880657E-5 0.0 0.0 0.0 0.0 42-43 7.861595046880657E-5 0.0 0.0 0.0 0.0 44-45 7.861595046880657E-5 0.0 0.0 0.0 0.0 46-47 7.861595046880657E-5 0.0 0.0 0.0 0.0 48-49 7.861595046880657E-5 0.0 0.0 0.0 0.0 50-51 7.861595046880657E-5 0.0 0.0 0.0 0.0 52-53 7.861595046880657E-5 0.0 0.0 0.0 0.0 54-55 9.826993808600821E-5 0.0 0.0 0.0 0.0 56-57 1.768858885548148E-4 0.0 1.1792392570320985E-4 0.0 0.0 58-59 1.9653987617201642E-4 0.0 2.358478514064197E-4 0.0 0.0 60-61 2.358478514064197E-4 0.0 2.358478514064197E-4 0.0 0.0 62-63 2.358478514064197E-4 0.0 2.358478514064197E-4 0.0 0.0 64-65 2.5550183902362135E-4 0.0 2.5550183902362135E-4 0.0 0.0 66-67 2.9480981425802466E-4 0.0 2.75155826640823E-4 0.0 0.0 68-69 3.9307975234403284E-4 0.0 2.75155826640823E-4 0.0 0.0 70-71 4.323877275784361E-4 0.0 2.75155826640823E-4 0.0 0.0 72-73 5.110036780472427E-4 0.0 2.75155826640823E-4 0.0 0.0 74-75 6.289276037504525E-4 0.0 2.75155826640823E-4 0.0 0.0 76-77 8.058134923052674E-4 0.0 2.75155826640823E-4 0.0 0.0 78-79 9.433914056256788E-4 0.0 2.75155826640823E-4 0.0 0.0 80-81 0.0010023533684772837 0.0 2.75155826640823E-4 0.0 0.0 82-83 0.0010613153313288885 0.0 2.75155826640823E-4 0.0 0.0 84-85 0.001100623306563292 0.0 2.75155826640823E-4 0.0 0.0 86-87 0.001100623306563292 3.9307975234403285E-5 2.75155826640823E-4 0.0 0.0 88 0.001100623306563292 3.9307975234403285E-5 2.75155826640823E-4 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11185 0.0 82.75557 8 CACCGGC 11450 0.0 79.487465 9 CTTCACC 12660 0.0 76.74727 6 CGGGCCC 795 0.0 73.802185 7 CCTTCAC 14055 0.0 73.2042 5 GTGTGGC 4065 0.0 71.70631 7 CGTAGTG 2320 0.0 71.014244 4 GGCTGCA 30 7.5718555E-5 70.65673 1 CAGCTGC 45 9.605719E-8 70.65672 1 GGGCCCG 830 0.0 70.12452 8 TTCACCG 13750 0.0 69.605064 7 AACGGGC 845 0.0 67.213264 5 GCAGCCT 80 0.0 66.24068 1 GGCCCGA 865 0.0 66.201836 9 GCGTAGT 1490 0.0 66.154495 3 ACCTTCA 5395 0.0 64.73033 4 CGGCTGC 25 0.0032559326 63.591053 1 GCCTTCA 4200 0.0 62.919525 4 TGTGGCA 4700 0.0 62.817276 8 ACGGGCC 920 0.0 62.244118 6 >>END_MODULE