FastQCFastQC Report
Tue 7 Mar 2017
1HL_8A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_8A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2544013
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG111850.082.755578
CACCGGC114500.079.4874659
CTTCACC126600.076.747276
CGGGCCC7950.073.8021857
CCTTCAC140550.073.20425
GTGTGGC40650.071.706317
CGTAGTG23200.071.0142444
GGCTGCA307.5718555E-570.656731
CAGCTGC459.605719E-870.656721
GGGCCCG8300.070.124528
TTCACCG137500.069.6050647
AACGGGC8450.067.2132645
GCAGCCT800.066.240681
GGCCCGA8650.066.2018369
GCGTAGT14900.066.1544953
ACCTTCA53950.064.730334
CGGCTGC250.003255932663.5910531
GCCTTCA42000.062.9195254
TGTGGCA47000.062.8172768
ACGGGCC9200.062.2441186
Produced by FastQC (version 0.11.5)