FastQCFastQC Report
Tue 7 Mar 2017
1HL_9A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_9A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2832945
Sequences flagged as poor quality0
Sequence length93
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA142800.5040690871160577No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG75330.2659070331404246No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA69290.24458646390946526No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG66520.2348086531859955No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT58640.20699307610984327No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA58160.20529872623718426No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA51180.18066005517226774No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG50770.17921279798937148No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG50130.1769536648258261No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT48030.16954088413294294No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA47860.1689408018863762No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG47670.16827012172844866No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC45910.16205750552869894No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC44860.15835111518225733No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA41850.1477261295224581No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA40680.14359615170785173No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT38470.13579508250248415No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG38440.13568918563544297No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA36940.13039434228338356No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34640.12227558247689242No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC32730.11553348194193674No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA31060.10963855634331057No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA30690.10833249498313592No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC29100.10271996102995294No Hit
CAGCCTTATTCAGGACACATCAGTACAAGTGACAGCCCTTCAACCGCTGA28450.10042552891072716No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCCA700.086.999633
GCTGCAT750.086.999633
GCAGCTT257.8038283E-786.999633
GCAGCCC257.8038283E-786.999633
GCAGCAC600.086.999633
TGCAGCG159.388695E-486.999632
AGCAGCC850.086.999632
GCTGCTC202.6992671E-586.9996263
GCTGCCT450.086.9996263
GCTGCAC402.0008883E-1186.9996263
CTGCAGC1650.086.9996261
AGCAGCG1100.086.9996262
CAGCTGC6050.086.2806241
CAGCAGC5200.085.326561
AGCTGCC2450.085.224132
GCTGCCC1950.084.768873
AGCTGCT1450.083.999632
AGCAGCA2150.082.953132
AGCTGCA1650.081.726922
CAGCCCT159100.081.4220351
Produced by FastQC (version 0.11.5)