FastQCFastQC Report
Tue 7 Mar 2017
1HL_9A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1HL_9A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2832945
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG111800.079.0978558
CACCGGC112550.075.109329
CTTCACC124900.074.033696
CCTTCAC137500.070.76565
GTGTGGC44500.069.0886847
CGTAGTG25200.066.495654
TTCACCG138700.066.1600657
AGCAGCC506.1227183E-965.7182
GCTGCCA1150.065.309812
AGGCAGC301.3799935E-462.588572
ACCTTCA53050.062.289224
TGTGGCA50650.061.8118868
CGGGCCC7000.061.0195477
AGGCGCG6550.060.9120525
GGGCCCG6950.060.783178
CAGCGTA12300.059.859391
ATGCAGC950.059.294432
GAGCTGC457.952442E-658.8545531
CGCTGCA901.8189894E-1258.8545531
GCCTTCA39400.058.6130754
Produced by FastQC (version 0.11.5)