##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1HL_9A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2832945 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.790415274564102 12.0 12.0 12.0 2.0 27.0 2 14.597786049499726 12.0 12.0 12.0 12.0 22.0 3 13.727147897329457 12.0 12.0 12.0 12.0 22.0 4 13.699263487289729 12.0 12.0 12.0 12.0 22.0 5 22.429037626921808 22.0 22.0 22.0 22.0 27.0 6 26.730608606944365 27.0 22.0 32.0 22.0 32.0 7 34.91679259569106 37.0 37.0 37.0 27.0 41.0 8 37.55073995435845 41.0 37.0 41.0 27.0 41.0 9 38.50724564013774 41.0 37.0 41.0 32.0 41.0 10-11 38.866662783781535 41.0 41.0 41.0 37.0 41.0 12-13 39.04670298929206 41.0 41.0 41.0 37.0 41.0 14-15 39.00242468526569 41.0 41.0 41.0 37.0 41.0 16-17 38.911215007704 41.0 41.0 41.0 37.0 41.0 18-19 38.84786732534518 41.0 41.0 41.0 37.0 41.0 20-21 38.85557979417179 41.0 41.0 41.0 37.0 41.0 22-23 38.8445012169315 41.0 41.0 41.0 34.5 41.0 24-25 38.86426986757597 41.0 41.0 41.0 37.0 41.0 26-27 38.82866186953859 41.0 41.0 41.0 37.0 41.0 28-29 38.78233040175506 41.0 41.0 41.0 34.5 41.0 30-31 38.65325447546634 41.0 41.0 41.0 32.0 41.0 32-33 38.70522283348247 41.0 41.0 41.0 32.0 41.0 34-35 38.67987024103892 41.0 41.0 41.0 32.0 41.0 36-37 38.599800560900405 41.0 41.0 41.0 32.0 41.0 38-39 38.50622550031858 41.0 41.0 41.0 32.0 41.0 40-41 38.556338015739804 41.0 41.0 41.0 32.0 41.0 42-43 38.28935577640935 41.0 41.0 41.0 32.0 41.0 44-45 38.39283519447077 41.0 41.0 41.0 32.0 41.0 46-47 38.40923967814412 41.0 41.0 41.0 32.0 41.0 48-49 38.35195247348607 41.0 41.0 41.0 32.0 41.0 50-51 38.23254722559033 41.0 41.0 41.0 32.0 41.0 52-53 38.19928501965269 41.0 41.0 41.0 32.0 41.0 54-55 38.07899129704248 41.0 37.0 41.0 32.0 41.0 56-57 37.87044647884092 41.0 37.0 41.0 27.0 41.0 58-59 37.869010517323844 41.0 37.0 41.0 29.5 41.0 60-61 37.87391495422608 41.0 37.0 41.0 29.5 41.0 62-63 37.86811092343833 41.0 37.0 41.0 29.5 41.0 64-65 37.847971810253995 41.0 37.0 41.0 29.5 41.0 66-67 37.722254579598264 41.0 37.0 41.0 27.0 41.0 68-69 37.74434713698995 41.0 37.0 41.0 27.0 41.0 70-71 37.72942415048651 41.0 37.0 41.0 27.0 41.0 72-73 37.587517759787076 41.0 37.0 41.0 27.0 41.0 74-75 37.501435961517075 41.0 37.0 41.0 27.0 41.0 76-77 36.46546544320486 39.0 34.5 41.0 24.5 41.0 78-79 36.98919693110879 41.0 37.0 41.0 27.0 41.0 80-81 37.63370979669566 41.0 37.0 41.0 27.0 41.0 82-83 37.82592320006212 41.0 37.0 41.0 29.5 41.0 84-85 37.73487713316001 41.0 37.0 41.0 27.0 41.0 86-87 37.592509738099395 41.0 37.0 41.0 27.0 41.0 88-89 37.51564449715755 41.0 37.0 41.0 27.0 41.0 90-91 37.35792682173498 41.0 37.0 41.0 27.0 41.0 92-93 37.24977576338404 41.0 37.0 41.0 27.0 41.0 94-95 37.08633383281356 41.0 37.0 41.0 27.0 41.0 96-97 37.031825714936225 41.0 37.0 41.0 27.0 41.0 98-99 36.91049773292457 41.0 37.0 41.0 27.0 41.0 100 36.78827403991253 41.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.7351838600971572 1101 2 -0.6051549248119237 1101 3 0.05874315211354286 1101 4 0.1867719106973187 1101 5 -1.070149249511612 1101 6 -0.9245597715586484 1101 7 -0.2280058750320535 1101 8 -0.2826296559189956 1101 9 -0.3000435735141025 1101 10-11 -0.23520069795038268 1101 12-13 -0.14238215100449025 1101 14-15 -0.18648837497377002 1101 16-17 -0.17896733876795423 1101 18-19 -0.0662705738387146 1101 20-21 -0.2133397982882954 1101 22-23 -0.20692158554751217 1101 24-25 -0.11781212623477444 1101 26-27 -0.12817154354986116 1101 28-29 -0.26200262121265894 1101 30-31 -0.08454866529762484 1101 32-33 -0.30986908259174584 1101 34-35 -0.25977833301793396 1101 36-37 -0.12570719960132237 1101 38-39 -0.20116252051221295 1101 40-41 -0.18270235960572023 1101 42-43 -0.10369686960385849 1101 44-45 -0.08107271476625044 1101 46-47 -0.12003868976225363 1101 48-49 -0.1817307985095269 1101 50-51 -0.14882547362589804 1101 52-53 -0.14819958968300995 1101 54-55 -0.12254318669540964 1101 56-57 -0.10207889626036604 1101 58-59 -0.08012205235808523 1101 60-61 -0.34356650490644114 1101 62-63 -0.34159513522840257 1101 64-65 -0.245789679591482 1101 66-67 -0.1379890614248538 1101 68-69 -0.03480681189393664 1101 70-71 -0.19223333187913738 1101 72-73 -0.11753845295142185 1101 74-75 -0.010216571578695266 1101 76-77 -0.47837232704690535 1101 78-79 -0.309368009505981 1101 80-81 -0.2824436438805549 1101 82-83 -0.22785306903639224 1101 84-85 -0.07431812510785818 1101 86-87 -0.2703532081846731 1101 88-89 -0.46895250242951647 1101 90-91 -0.3933707013282586 1101 92-93 -0.21573735329803156 1101 94-95 0.06933160067012523 1101 96-97 -0.24119028864596004 1101 98-99 -0.39212434496755577 1101 100 -0.8665854762846052 1102 1 1.8290152808422047 1102 2 0.7292408301525466 1102 3 -0.005743798085662277 1102 4 0.11798145684289452 1102 5 -0.7865628396545326 1102 6 -0.036680322784743424 1102 7 -0.09017262951824989 1102 8 -0.18407022156530672 1102 9 -0.21252987854778382 1102 10-11 -0.2416557934219199 1102 12-13 -0.26533762674286265 1102 14-15 -0.1358383211059646 1102 16-17 -0.2148704320902155 1102 18-19 -0.1954306473735059 1102 20-21 -0.13210166612569196 1102 22-23 -0.16209602265579548 1102 24-25 -0.22451677531481806 1102 26-27 -0.1739104508871634 1102 28-29 -0.15201222028997563 1102 30-31 -0.22345934683530544 1102 32-33 -0.21381822524324434 1102 34-35 -0.09908459446774032 1102 36-37 -0.1857782382138069 1102 38-39 -0.15994161196490353 1102 40-41 -0.1973243513334566 1102 42-43 -0.010276677875133089 1102 44-45 -0.06527872034200755 1102 46-47 -0.34716503579723224 1102 48-49 -0.29168551691446254 1102 50-51 -0.09598214500062596 1102 52-53 -0.014446392057386959 1102 54-55 -0.07660034496849732 1102 56-57 -0.1613787604587813 1102 58-59 -0.17295372256072739 1102 60-61 -0.24075637062336597 1102 62-63 -0.21629613413088578 1102 64-65 -0.2091894970826047 1102 66-67 -0.08532390537144607 1102 68-69 -0.2942844006325984 1102 70-71 -0.3044360489028506 1102 72-73 -0.23068195635702438 1102 74-75 -0.39619540624526906 1102 76-77 -0.1384703435629504 1102 78-79 -0.26698981930088195 1102 80-81 -0.15160017156514272 1102 82-83 -0.22542329759171764 1102 84-85 -0.20295976206176647 1102 86-87 -0.19824292008274114 1102 88-89 -0.5003935209077852 1102 90-91 -0.4624876582207378 1102 92-93 -0.28117397050573345 1102 94-95 -0.2603251799123072 1102 96-97 -0.2693318743162436 1102 98-99 -0.3684456341977267 1102 100 -0.3809321685872078 1103 1 1.6145948261491245 1103 2 0.7885549231997082 1103 3 0.37959711180015177 1103 4 1.0217506573509443 1103 5 2.8920234953031247 1103 6 -0.17341685349073188 1103 7 -0.36358673159251964 1103 8 -0.0346099659882384 1103 9 -0.2066959020469099 1103 10-11 -0.25379725319515956 1103 12-13 -0.29397229503329925 1103 14-15 -0.35730266786148235 1103 16-17 -0.405687415310787 1103 18-19 -0.42004249007266026 1103 20-21 -0.28584929817545657 1103 22-23 -0.27094645214292257 1103 24-25 -0.4447316504559282 1103 26-27 -0.24035046275896832 1103 28-29 -0.3126843267605395 1103 30-31 -0.033391596893515896 1103 32-33 -0.3032000815563407 1103 34-35 -0.32029878041750237 1103 36-37 -0.24744046183358392 1103 38-39 -0.05896425268368688 1103 40-41 -0.12773542782769454 1103 42-43 -0.12645180576193837 1103 44-45 -0.5338697154190726 1103 46-47 -0.44559681734521916 1103 48-49 -0.25674453121426666 1103 50-51 -0.13241476863452561 1103 52-53 -0.022935703928631312 1103 54-55 -0.37452071255498254 1103 56-57 -0.2666460777656141 1103 58-59 -0.23847053779714855 1103 60-61 -0.250031390617913 1103 62-63 -0.36548815803173085 1103 64-65 -0.45334685988805745 1103 66-67 -0.4037263971196339 1103 68-69 -0.22949783176959215 1103 70-71 -0.45310002031723684 1103 72-73 -0.41708223563108504 1103 74-75 -0.2551814921746711 1103 76-77 -0.4288600714472821 1103 78-79 -0.36732387224903107 1103 80-81 -0.18795526533548212 1103 82-83 -0.1638630869503217 1103 84-85 -0.4335043464800421 1103 86-87 -0.3864725210679296 1103 88-89 -0.38403435328039137 1103 90-91 -0.6897535871517491 1103 92-93 -0.30215275213934234 1103 94-95 -0.26910570760838226 1103 96-97 -0.2791069660946093 1103 98-99 -0.43141936997794517 1103 100 -0.17468857458587195 1104 1 1.9242595980502095 1104 2 -0.5854080361564353 1104 3 0.026636434967297618 1104 4 0.13836511902362858 1104 5 -1.1706811947419524 1104 6 -0.38437983552536537 1104 7 -0.1735883457673708 1104 8 -0.1824123536329978 1104 9 -0.09827237724931592 1104 10-11 -0.1425550922819312 1104 12-13 0.0647607244475239 1104 14-15 -0.04250838844381377 1104 16-17 -0.007854845264269272 1104 18-19 0.023236772849742238 1104 20-21 0.04769008702402999 1104 22-23 -0.08856136871943221 1104 24-25 0.049283062547779366 1104 26-27 -0.18866084857344134 1104 28-29 -0.15790346767266783 1104 30-31 -0.1520507912201623 1104 32-33 -0.01956354570899066 1104 34-35 -0.09798521427835283 1104 36-37 -0.021513845424230738 1104 38-39 -0.15730435398983644 1104 40-41 -0.16397165231273192 1104 42-43 -0.06911257957867889 1104 44-45 -0.01016919287998519 1104 46-47 -0.3184345234026509 1104 48-49 -0.3909728422404086 1104 50-51 -0.2454536995971992 1104 52-53 -0.04289977853286331 1104 54-55 -0.16160721365088904 1104 56-57 -0.15022871884916356 1104 58-59 -0.20947658895182286 1104 60-61 -0.16750503354375468 1104 62-63 -0.2163140552661531 1104 64-65 -0.25184847873201477 1104 66-67 -0.4452140260034909 1104 68-69 -0.43935256937279377 1104 70-71 -0.32715019180663774 1104 72-73 -0.20622530888661572 1104 74-75 -0.3100412590664803 1104 76-77 -0.3442633016646326 1104 78-79 -0.2202890514291127 1104 80-81 -0.39698857470076376 1104 82-83 -0.34767908302294614 1104 84-85 -0.3539753853816592 1104 86-87 -0.33751449761837193 1104 88-89 -0.40870654905178583 1104 90-91 -0.1861705525297026 1104 92-93 -0.39852816251994483 1104 94-95 -0.22722353970484477 1104 96-97 -0.26135371073081615 1104 98-99 -0.3308217584231272 1104 100 -0.17566641770141445 1105 1 2.0547054785025516 1105 2 0.32343849049946094 1105 3 -0.07222947779522393 1105 4 -0.19333439632370464 1105 5 -1.109547107504472 1105 6 0.6210508591434554 1105 7 0.32573184809369593 1105 8 0.07243611163356434 1105 9 0.052145716450198165 1105 10-11 -0.03410113428516581 1105 12-13 -4.4347587557780344E-4 1105 14-15 -0.02347607826610698 1105 16-17 -0.039668092437125324 1105 18-19 -0.02020813829727075 1105 20-21 -0.03956193236207639 1105 22-23 -0.11469707146579822 1105 24-25 -0.0229162912486629 1105 26-27 -0.01724889380768957 1105 28-29 -0.03836938043518501 1105 30-31 0.14727941879599626 1105 32-33 -0.05394706751835798 1105 34-35 -0.019306377444756606 1105 36-37 -0.10859042054539003 1105 38-39 0.048574805945541755 1105 40-41 0.13604805689566746 1105 42-43 0.18322086145524707 1105 44-45 0.2174579476692884 1105 46-47 -0.14454793212640027 1105 48-49 0.016253652105305605 1105 50-51 0.11267424870651155 1105 52-53 0.07341524569977054 1105 54-55 0.013135274232794814 1105 56-57 0.15292023107006258 1105 58-59 0.006252005555445805 1105 60-61 0.15820675967110986 1105 62-63 -0.21917189048424746 1105 64-65 -0.05304330910358601 1105 66-67 -0.05407767705357713 1105 68-69 -0.1955558003905722 1105 70-71 -0.1405634389794983 1105 72-73 -0.11543500193992173 1105 74-75 -0.12588487780638502 1105 76-77 -0.13234892363555417 1105 78-79 -0.06435690280229522 1105 80-81 -0.26757145030657625 1105 82-83 -0.07664838835412269 1105 84-85 0.04160135129038878 1105 86-87 -0.21970092734373736 1105 88-89 -0.10924095939104461 1105 90-91 -0.04753888855555033 1105 92-93 -0.03778082810961081 1105 94-95 -0.045012231142642634 1105 96-97 -0.20364288682128517 1105 98-99 -0.08028444016787262 1105 100 -0.23879856632823504 1106 1 1.3713904100094005 1106 2 -0.6667822991862771 1106 3 -0.1830900740885344 1106 4 -0.1360354438660103 1106 5 -1.2514794201555617 1106 6 -0.7083547814689553 1106 7 -0.34499240653966723 1106 8 -0.25703753864846846 1106 9 -0.24633389676494488 1106 10-11 -0.3032485960095812 1106 12-13 -0.21752276677002413 1106 14-15 -0.2666138703692553 1106 16-17 -0.17823489340408827 1106 18-19 -0.09124875070661886 1106 20-21 -0.2240292973902811 1106 22-23 -0.1609162495479879 1106 24-25 -0.28250565466526467 1106 26-27 -0.34573882128553635 1106 28-29 -0.2672069307977978 1106 30-31 -0.22197166903639953 1106 32-33 -0.37940025043536707 1106 34-35 -0.33325947978157444 1106 36-37 -0.49791306357520426 1106 38-39 -0.19218570976759253 1106 40-41 -0.3784610486643558 1106 42-43 -0.16975383636908958 1106 44-45 -0.3343968468512628 1106 46-47 -0.4221596968322814 1106 48-49 -0.37092185152080503 1106 50-51 -0.5772103604796328 1106 52-53 -0.419381692253495 1106 54-55 -0.44486101907904896 1106 56-57 -0.3886581734424297 1106 58-59 -0.3017085101576882 1106 60-61 -0.2112923539469449 1106 62-63 -0.3506457019266378 1106 64-65 -0.471428160030257 1106 66-67 -0.5568839623074027 1106 68-69 -0.4712832782310201 1106 70-71 -0.6280701271342082 1106 72-73 -0.4809054290148609 1106 74-75 -0.5367343540352323 1106 76-77 -0.517784056592852 1106 78-79 -0.41631741851542614 1106 80-81 -0.4333964301615296 1106 82-83 -0.6010456486280944 1106 84-85 -0.6550489307402287 1106 86-87 -0.5687761892293111 1106 88-89 -0.5862182357810468 1106 90-91 -0.6597725710696523 1106 92-93 -0.4888415855632715 1106 94-95 -0.6070051400870469 1106 96-97 -0.686839502453843 1106 98-99 -0.7973967689350019 1106 100 -0.8955324905828661 1107 1 1.307174653842825 1107 2 -0.6550210771546627 1107 3 -0.2170189093404602 1107 4 -0.2781926829530086 1107 5 -1.2061700058231288 1107 6 -0.24415618649305415 1107 7 -0.3726374073106271 1107 8 -0.4590892686706809 1107 9 -0.33891497210063903 1107 10-11 -0.1713453296414471 1107 12-13 -0.1911645327210536 1107 14-15 -0.38078272358236376 1107 16-17 -0.2983827441585021 1107 18-19 -0.21631270194819052 1107 20-21 -0.3179334295598366 1107 22-23 -0.3781174077348126 1107 24-25 -0.3573082848435334 1107 26-27 -0.38549068724243796 1107 28-29 -0.36142670629588025 1107 30-31 -0.35007318328567294 1107 32-33 -0.1895344173982636 1107 34-35 -0.29560437584347454 1107 36-37 -0.19667416754298728 1107 38-39 -0.2284053381697504 1107 40-41 -0.2198816468673357 1107 42-43 -0.11744559170728763 1107 44-45 -0.17377519884792747 1107 46-47 -0.30487763588941164 1107 48-49 -0.3661620404487351 1107 50-51 -0.3268014918859663 1107 52-53 -0.22900989441232156 1107 54-55 -0.3244568794901852 1107 56-57 -0.11988457277301023 1107 58-59 -0.27848058051340985 1107 60-61 -0.24309636282689695 1107 62-63 -0.5226955093793606 1107 64-65 -0.33023978628533257 1107 66-67 -0.30254156816646827 1107 68-69 -0.6679050397820774 1107 70-71 -0.4437389393798128 1107 72-73 -0.24616614685585603 1107 74-75 -0.25969096267427716 1107 76-77 -0.4695586914498051 1107 78-79 -0.5954513783827551 1107 80-81 -0.6169655255667834 1107 82-83 -0.3186443851515577 1107 84-85 -0.3264747095582905 1107 86-87 -0.3328983652940991 1107 88-89 -0.36452774882258865 1107 90-91 -0.15758532570526995 1107 92-93 -0.36366521561961207 1107 94-95 -0.22552848414505178 1107 96-97 -0.33709604935130955 1107 98-99 -0.5714050166290434 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2223 16-17 0.15439030630698625 2223 18-19 0.06019248964621937 2223 20-21 0.15055894675879955 2223 22-23 0.22481470247454638 2223 24-25 0.24316251565871738 2223 26-27 0.21522049394741316 2223 28-29 0.16117253479244198 2223 30-31 0.20351301377244368 2223 32-33 0.23205481631210034 2223 34-35 0.14165029445477728 2223 36-37 0.048511934242675636 2223 38-39 0.15042472745260937 2223 40-41 0.081180552971027 2223 42-43 0.29548742346465673 2223 44-45 0.32330943903506437 2223 46-47 0.20929288420013137 2223 48-49 0.31102822039415656 2223 50-51 0.30241114038626193 2223 52-53 0.2714105361236321 2223 54-55 0.2063553684243189 2223 56-57 0.3541343597984721 2223 58-59 0.5089160557909267 2223 60-61 0.22234522120957223 2223 62-63 0.3404230859678705 2223 64-65 0.2012672089497869 2223 66-67 0.4821888849558391 2223 68-69 0.43563792017457814 2223 70-71 0.4506758074883237 2223 72-73 0.5119565208230341 2223 74-75 0.45788372501935726 2223 76-77 0.4932005269138884 2223 78-79 0.6309633483751043 2223 80-81 0.3061623016557462 2223 82-83 0.21559807946377418 2223 84-85 0.10141139838615487 2223 86-87 0.33893831912408245 2223 88-89 0.4095791348004809 2223 90-91 0.6345662919782029 2223 92-93 0.49417395995946123 2223 94-95 0.4935711283549935 2223 96-97 0.5337134711065019 2223 98-99 0.5642056697222344 2223 100 0.5918017476435082 2224 1 1.295676193067175 2224 2 -0.8000070385907385 2224 3 -0.049245487166562185 2224 4 -0.27425278757712235 2224 5 -1.0542554245681188 2224 6 0.554381433137987 2224 7 0.3142344642592505 2224 8 0.19343853256288668 2224 9 0.16597734472041026 2224 10-11 0.20125536708148672 2224 12-13 0.16203079198853487 2224 14-15 0.12532751408063092 2224 16-17 0.18083585133484092 2224 18-19 0.15716812097645771 2224 20-21 0.12222877439310054 2224 22-23 0.1875215931183476 2224 24-25 0.16587386884504696 2224 26-27 0.16518921630554928 2224 28-29 0.039789385671795685 2224 30-31 0.03220616616030014 2224 32-33 0.12850689577722818 2224 34-35 0.13712400131354485 2224 36-37 -0.1009712873942803 2224 38-39 -0.0473485833555074 2224 40-41 0.041818537176808945 2224 42-43 0.24314917070943665 2224 44-45 0.2388996501292624 2224 46-47 0.1500088035237397 2224 48-49 0.13802998947351597 2224 50-51 0.12964699372798805 2224 52-53 0.18254019689070589 2224 54-55 0.19511832772752058 2224 56-57 0.3485372556698252 2224 58-59 0.25946988919465497 2224 60-61 0.2586103520178469 2224 62-63 0.2663458760132187 2224 64-65 0.21416574985775583 2224 66-67 0.2984078364177307 2224 68-69 0.24443490636461007 2224 70-71 0.3513882205285128 2224 72-73 0.4046841702584345 2224 74-75 0.27280774226390037 2224 76-77 0.4875944578560478 2224 78-79 0.45704372195366005 2224 80-81 0.2784009042424245 2224 82-83 0.3153598115677809 2224 84-85 0.09718393621815125 2224 86-87 0.3666519035816833 2224 88-89 0.38365056735632663 2224 90-91 0.43706790566545806 2224 92-93 0.4292459583019479 2224 94-95 0.6458713299662975 2224 96-97 0.41082919439698884 2224 98-99 0.4487025180515758 2224 100 0.5425982317974984 2225 1 1.165128024817097 2225 2 1.7592098740233073 2225 3 0.8555017007621277 2225 4 0.9513467231086956 2225 5 3.228345679251536 2225 6 -0.09640550664290615 2225 7 0.1685534484090283 2225 8 0.4164969709161781 2225 9 0.12313018629647843 2225 10-11 0.14868010339713322 2225 12-13 0.2991395749718819 2225 14-15 0.20926416611701626 2225 16-17 0.18105725245842308 2225 18-19 0.08575567723288913 2225 20-21 0.25443964430328236 2225 22-23 0.2752099052783876 2225 24-25 0.16776451285066685 2225 26-27 0.03312871754430091 2225 28-29 0.11385694987620099 2225 30-31 0.19227579246568638 2225 32-33 0.33288706728652784 2225 34-35 0.3385215295319881 2225 36-37 0.26532136502812165 2225 38-39 0.2050917156814549 2225 40-41 0.22230324018301673 2225 42-43 0.36619240264563047 2225 44-45 0.3269602732506769 2225 46-47 0.459045524712387 2225 48-49 0.3643326430394822 2225 50-51 0.29250964484368325 2225 52-53 0.22574989292089498 2225 54-55 0.21825793879724387 2225 56-57 0.47194995121196115 2225 58-59 0.3822263847434684 2225 60-61 0.33131326341568723 2225 62-63 0.2471217634377041 2225 64-65 0.35179736886252044 2225 66-67 0.5297858625601393 2225 68-69 0.38827368325153344 2225 70-71 0.4853330529400921 2225 72-73 0.5172919984542403 2225 74-75 0.4257826823039821 2225 76-77 0.6356743321783966 2225 78-79 0.5600888993262103 2225 80-81 0.31157651973678213 2225 82-83 0.18916201763720863 2225 84-85 0.23135263233098868 2225 86-87 0.4312423915368271 2225 88-89 0.42635951360855984 2225 90-91 0.3836105004866255 2225 92-93 0.42116485576898555 2225 94-95 0.6637767321998851 2225 96-97 0.6269724068326354 2225 98-99 0.51447519719909 2225 100 0.6175169244088536 2226 1 1.6884793021482611 2226 2 1.4744009262448134 2226 3 0.8320481022759516 2226 4 1.0546497871480227 2226 5 3.111401093365437 2226 6 -0.42890390558568825 2226 7 0.28149807980583574 2226 8 0.08464678779014889 2226 9 0.19117663776297178 2226 10-11 0.10780159406595402 2226 12-13 0.16735304448820187 2226 14-15 0.09900474419335836 2226 16-17 0.0281070756134838 2226 18-19 0.18258086921558458 2226 20-21 0.27514506645168524 2226 22-23 0.10716848804388945 2226 24-25 0.22066314726458103 2226 26-27 0.18632405992896395 2226 28-29 0.10050345467750788 2226 30-31 0.16491824837289926 2226 32-33 0.10090046114909512 2226 34-35 0.08134202113176059 2226 36-37 0.27139431650482493 2226 38-39 0.225719052683381 2226 40-41 0.1876019517157559 2226 42-43 0.3646916404192595 2226 44-45 0.28333608131895005 2226 46-47 0.28558342414090276 2226 48-49 0.14930181343389393 2226 50-51 -0.00377462436584608 2226 52-53 0.33839784751867086 2226 54-55 0.41296738178518666 2226 56-57 0.24881299500837173 2226 58-59 0.2588415666467867 2226 60-61 0.2251701444081604 2226 62-63 0.24728876590167204 2226 64-65 0.28220129350614087 2226 66-67 0.26526222013899314 2226 68-69 0.396362665406194 2226 70-71 0.5069532869991491 2226 72-73 0.24931897196280772 2226 74-75 0.23101120760286875 2226 76-77 0.2741655089464885 2226 78-79 0.3510337654460258 2226 80-81 0.2166724405593783 2226 82-83 0.13483322493176075 2226 84-85 0.3128876304521171 2226 86-87 0.31385953133558075 2226 88-89 0.27806342969554976 2226 90-91 0.1312835709145972 2226 92-93 0.31002795006357786 2226 94-95 0.43619741693959924 2226 96-97 0.3306817474215009 2226 98-99 0.4079039346838442 2226 100 0.38433737673226176 2227 1 1.1256559735520568 2227 2 1.7105275994103533 2227 3 0.5707606212146779 2227 4 -0.05780964384845788 2227 5 3.2298786350697846 2227 6 0.14979937399494148 2227 7 0.10596155106399152 2227 8 0.17040838886128995 2227 9 0.24971799367792613 2227 10-11 0.22894837066532858 2227 12-13 0.06075058353036411 2227 14-15 0.11242491183289616 2227 16-17 0.05552596696881551 2227 18-19 0.11805522803935986 2227 20-21 0.12148757258842124 2227 22-23 0.014629661207465006 2227 24-25 0.07189162954341555 2227 26-27 0.27922635371706406 2227 28-29 0.020329629465805965 2227 30-31 0.19929328018213255 2227 32-33 0.1067321404024284 2227 34-35 0.05402233542653789 2227 36-37 0.04286106460312311 2227 38-39 0.13024015248019083 2227 40-41 0.05353518560854553 2227 42-43 0.3219574951186104 2227 44-45 0.27151933380790183 2227 46-47 0.2559649391607337 2227 48-49 0.15860216695678986 2227 50-51 0.170844545736216 2227 52-53 0.2988508892641235 2227 54-55 0.2349431950248757 2227 56-57 0.47572419146609235 2227 58-59 0.2974500253574277 2227 60-61 0.2140245814532804 2227 62-63 0.2803429610008976 2227 64-65 0.0639705522825551 2227 66-67 0.20187677839196994 2227 68-69 0.3120105362431005 2227 70-71 0.41345834319872665 2227 72-73 0.434596681228399 2227 74-75 0.25237056773816846 2227 76-77 0.38383919517632137 2227 78-79 0.3858114140293907 2227 80-81 0.13899488632709023 2227 82-83 0.11793577006171319 2227 84-85 0.19731422257752484 2227 86-87 0.1721168944384459 2227 88-89 0.18762240694559296 2227 90-91 0.09640170550385818 2227 92-93 0.2760765976329651 2227 94-95 0.43404519459992486 2227 96-97 0.46668384585198197 2227 98-99 0.4579294212182674 2227 100 0.4067974732757236 2228 1 1.2486016427799633 2228 2 1.76077905617168 2228 3 -0.24049681603621487 2228 4 -0.0703092122865101 2228 5 3.1543978750604253 2228 6 -0.10652310443655466 2228 7 0.22221352186355148 2228 8 0.29730678191639726 2228 9 0.2110836474846849 2228 10-11 0.19395310825648693 2228 12-13 0.205797748456078 2228 14-15 0.24041621154403714 2228 16-17 0.12960838954659692 2228 18-19 0.06346198956015314 2228 20-21 0.21056050080336064 2228 22-23 0.2288604643730281 2228 24-25 0.2965736738811344 2228 26-27 0.23242332626205098 2228 28-29 0.1296417005411712 2228 30-31 0.14811839593663478 2228 32-33 0.0782639359686641 2228 34-35 0.11670377365946649 2228 36-37 0.18793983904391354 2228 38-39 0.2497775180726265 2228 40-41 0.133065569487691 2228 42-43 0.37796103968073425 2228 44-45 0.2708931278320108 2228 46-47 0.24963873970622785 2228 48-49 0.24973416353498834 2228 50-51 0.3736472049250281 2228 52-53 0.38862214225149216 2228 54-55 0.19334085346564933 2228 56-57 0.2980729625694778 2228 58-59 0.3522849385271627 2228 60-61 0.28419885575789294 2228 62-63 0.2827386093220241 2228 64-65 0.13416722944736392 2228 66-67 0.20080159454658286 2228 68-69 0.20626527671132777 2228 70-71 0.4610298194474751 2228 72-73 0.3728211778353625 2228 74-75 0.30109218534742865 2228 76-77 0.3500900535050846 2228 78-79 0.2630537907273478 2228 80-81 0.34211440788791947 2228 82-83 0.2067661796427771 2228 84-85 0.4065323857731542 2228 86-87 0.4572931059766816 2228 88-89 0.4854352126353021 2228 90-91 0.3424409642146955 2228 92-93 0.27610820714804873 2228 94-95 0.5482263585512683 2228 96-97 0.2705233278242005 2228 98-99 0.6283275706806393 2228 100 0.546192212439685 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 13.0 13 35.0 14 167.0 15 515.0 16 1866.0 17 4744.0 18 7897.0 19 9923.0 20 11739.0 21 13714.0 22 15576.0 23 17806.0 24 19905.0 25 22947.0 26 25518.0 27 28615.0 28 32610.0 29 36798.0 30 42064.0 31 49094.0 32 57558.0 33 68353.0 34 83354.0 35 107616.0 36 150691.0 37 249519.0 38 616119.0 39 1156947.0 40 1242.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.26613488034808 18.186144326185637 19.047021713877935 35.50069907958835 2 19.03431886321347 52.2597596617006 11.36172287280554 17.344198602280393 3 41.25470137965968 14.834174330952418 24.09379638503395 19.81732790435395 4 18.597819583507622 15.735109576783172 21.669993593239546 43.997077246469665 5 17.285863297734334 35.58237099555409 25.90452691457123 21.227238792140334 6 17.87772088762754 27.794256506921243 30.494591317515873 23.833431287935348 7 20.476147613172866 26.452754995243467 36.01908967523196 17.05200771635171 8 20.99956052800178 27.467917661655978 34.08746022248932 17.445061587852923 9 21.241428972323853 27.662379608499283 27.59425262403612 23.501938795140745 10-11 19.872358976259687 29.758943431658576 28.482144905742963 21.886552686338774 12-13 24.43171328776238 26.5680237350178 26.986475205131054 22.013787772088765 14-15 22.023600881767912 27.269325736998073 28.39308564056132 22.31398774067269 16-17 21.103692447258947 29.38450975927877 28.133903058478015 21.377894734984267 18-19 21.609297038947105 29.37612625730468 29.491483244468213 19.52309345928001 20-21 23.04451727795633 28.769549002892745 29.662383138394848 18.52355058075607 22-23 22.066612659264475 30.174323892627637 28.39954534945084 19.359518098657052 24-25 22.36084710433842 28.15458824650673 26.446365884265315 23.038198764889543 26-27 20.433435876799585 26.398447551929177 29.809879824705387 23.35823674656585 28-29 19.90877304081686 27.81326218449456 30.428536205125816 21.84942856956276 30-31 20.258658579077636 29.879868801438086 27.98567279507938 21.875799824404897 32-33 20.984258430714327 29.793165769190715 28.59243649276636 20.630139307328594 34-35 21.64097785167026 28.943131617451094 29.658729696481927 19.757160834396714 36-37 22.037049783882143 28.007303353930276 28.5638266891874 21.39182017300018 38-39 21.66230837765751 29.191070261172587 28.708828641583445 20.43779271958646 40-41 21.05228137083457 29.55961766414793 29.58776900859966 19.800331956417835 42-43 20.889182262949447 29.660211652911816 29.02290553276808 20.427700551370663 44-45 20.756244826496808 29.887096290256252 28.52640979616618 20.83024908708076 46-47 20.2228186692266 27.782140230468112 28.75480539019124 23.24023571011405 48-49 21.14952814120994 28.15296449454543 29.921283328832715 20.77622403541191 50-51 22.168150105279135 30.544980576749637 27.89286413961443 19.3940051783568 52-53 20.439431693711306 30.165070604103416 28.137702269998787 21.25779543218649 54-55 22.684493953440093 28.076617414615047 27.922792280993363 21.316096350951494 56-57 21.623475987811254 28.658622152298573 29.007804982924778 20.710096876965387 58-59 21.440495387078524 27.725330600796283 28.752009023727 22.082164988398194 60-61 19.81539313059688 30.11918011647974 29.33291186290986 20.732514890013515 62-63 20.480484752888536 30.941423986322196 27.140841592328798 21.437249668460474 64-65 20.193095979499766 30.085845340720702 28.57139579380951 21.149662885970017 66-67 20.443178388567375 29.993081404686645 29.225276170204506 20.338464036541477 68-69 21.1190827919356 29.77048619016606 29.01468260061526 20.095748417283072 70-71 19.74175284024222 30.460068938860445 29.31883605223539 20.47934216866194 72-73 20.29478157888699 30.45353863205957 28.391461888600027 20.860217900453414 74-75 20.250622585330813 28.986408137115266 30.207769653134815 20.555199624419114 76-77 19.78538234946319 30.143790295964095 28.547571520096575 21.52325583447614 78-79 19.682397646265635 29.283325302820916 28.29746076962313 22.736816281290317 80-81 21.205858302859948 29.114436557261342 28.175287374072806 21.504417765805904 82-83 20.429041156817377 27.83850374786662 29.714396149589916 22.01805894572609 84-85 20.99691310632575 28.8418412641262 28.21472001750828 21.946525612039768 86-87 21.489863022402485 28.45065823727605 29.65359369843043 20.405885041891036 88-89 20.8570056954865 27.913090441219296 29.520304841781257 21.70959902151295 90-91 21.033094535898154 28.130602606121897 28.959333838108396 21.87696901987155 92-93 20.772994180967157 27.739772568828553 29.679962018323685 21.807271231880605 94-95 21.27468058857479 27.908201535857565 28.83728769884343 21.97983017672422 96-97 20.343635333548658 28.447604877609695 28.895160336681442 22.313599452160208 98-99 22.32521280857906 28.716724115717035 28.281487992177752 20.676575083526156 100 20.98731179038068 29.946680927444763 28.067329228064786 20.998678054109767 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5.0 1 8.5 2 12.5 3 19.5 4 25.0 5 28.5 6 38.5 7 47.0 8 56.0 9 67.0 10 75.5 11 78.5 12 102.5 13 128.0 14 143.5 15 185.5 16 254.5 17 329.5 18 459.0 19 734.0 20 1152.5 21 1818.0 22 2813.5 23 4317.0 24 6641.5 25 9930.5 26 14187.5 27 19292.5 28 25125.5 29 31844.5 30 39508.5 31 48148.5 32 57993.5 33 69036.5 34 80886.0 35 93775.0 36 106614.5 37 116639.5 38 124001.0 39 129916.0 40 133984.0 41 135204.0 42 133620.5 43 131455.5 44 130509.0 45 127703.5 46 120252.5 47 110675.5 48 102311.5 49 98072.5 50 95715.0 51 89156.5 52 79076.5 53 68345.0 54 56909.0 55 46266.5 56 36502.0 57 27700.0 58 20468.5 59 15185.5 60 12352.0 61 10673.0 62 9192.0 63 7496.5 64 5992.0 65 5675.5 66 6527.5 67 6088.0 68 4214.5 69 3658.0 70 4403.5 71 4319.5 72 2754.0 73 1280.5 74 707.5 75 474.0 76 302.5 77 205.0 78 154.5 79 127.5 80 105.5 81 66.0 82 43.5 83 36.0 84 31.0 85 26.0 86 24.0 87 18.0 88 14.5 89 17.0 90 17.0 91 13.5 92 11.5 93 12.0 94 10.0 95 11.5 96 15.0 97 20.5 98 42.0 99 104.5 100 116.5 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.35870975257197 2 0.006636203667914484 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.7063903464416E-4 30-31 0.01030729505867569 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0010236697147314895 40-41 0.0015178550942570364 42-43 1.7649477840198097E-4 44-45 0.0 46-47 0.0020649889073031773 48-49 0.0 50-51 0.0 52-53 0.001270762404494263 54-55 0.017931869485641266 56-57 0.011701603808051339 58-59 0.014560819218163431 60-61 2.1179373408237718E-4 62-63 0.011525109029649358 64-65 5.2948433520594296E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.000411232833677E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2832945.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.50241889146263 #Duplication Level Percentage of deduplicated Percentage of total 1 85.8944456970427 63.13449528074021 2 8.644515687344562 12.7078562633004 3 2.464672595625588 5.434781926619413 4 1.0779579557314607 3.169300688382342 5 0.544149972470927 1.9998169608167966 6 0.3433280854194816 1.5141266851023958 7 0.23283345199793903 1.1979675344488439 8 0.15077568354623777 0.8865901960529742 9 0.1002563615209915 0.663217657294485 >10 0.5085262893683311 6.292960847240405 >50 0.027355561289054017 1.3469997217103684 >100 0.010643836055647674 1.3598551139404254 >500 5.388225870941457E-4 0.29203112435106243 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 1.7649477840198098E-5 0.0 0.0 0.0 0.0 14-15 3.5298955680396195E-5 0.0 0.0 0.0 0.0 16-17 3.5298955680396195E-5 0.0 0.0 0.0 0.0 18-19 3.5298955680396195E-5 0.0 0.0 0.0 0.0 20-21 3.5298955680396195E-5 0.0 0.0 0.0 0.0 22-23 3.5298955680396195E-5 0.0 0.0 0.0 0.0 24-25 3.5298955680396195E-5 0.0 0.0 0.0 0.0 26-27 3.5298955680396195E-5 0.0 0.0 0.0 0.0 28-29 3.5298955680396195E-5 0.0 0.0 0.0 0.0 30-31 3.5298955680396195E-5 0.0 0.0 0.0 0.0 32-33 3.5298955680396195E-5 0.0 0.0 0.0 0.0 34-35 3.5298955680396195E-5 0.0 0.0 0.0 0.0 36-37 3.5298955680396195E-5 0.0 0.0 0.0 0.0 38-39 3.5298955680396195E-5 0.0 0.0 0.0 0.0 40-41 3.5298955680396195E-5 0.0 0.0 0.0 0.0 42-43 3.5298955680396195E-5 0.0 0.0 0.0 0.0 44-45 5.2948433520594296E-5 0.0 0.0 0.0 0.0 46-47 7.059791136079239E-5 0.0 0.0 0.0 0.0 48-49 7.059791136079239E-5 0.0 0.0 0.0 0.0 50-51 7.059791136079239E-5 0.0 0.0 0.0 0.0 52-53 7.059791136079239E-5 0.0 0.0 0.0 0.0 54-55 8.824738920099048E-5 0.0 0.0 0.0 0.0 56-57 1.4119582272158478E-4 0.0 0.0 0.0 0.0 58-59 2.1179373408237718E-4 0.0 0.0 0.0 0.0 60-61 2.647421676029714E-4 0.0 0.0 0.0 0.0 62-63 2.8239164544316956E-4 0.0 0.0 0.0 0.0 64-65 3.176906011235658E-4 0.0 0.0 0.0 0.0 66-67 3.176906011235658E-4 0.0 0.0 0.0 0.0 68-69 3.176906011235658E-4 0.0 0.0 0.0 0.0 70-71 3.3534007896376386E-4 0.0 0.0 0.0 0.0 72-73 4.059379903245563E-4 0.0 0.0 0.0 0.0 74-75 4.5888642384515053E-4 0.0 0.0 0.0 0.0 76-77 4.941853795255467E-4 0.0 0.0 0.0 0.0 78-79 5.647832908863391E-4 0.0 0.0 0.0 0.0 80-81 8.471749363295087E-4 0.0 0.0 0.0 0.0 82-83 0.0011119171039324803 0.0 0.0 0.0 0.0 84-85 0.0012884118823344613 0.0 0.0 0.0 0.0 86-87 0.0013413603158550554 0.0 0.0 0.0 0.0 88 0.0013413603158550554 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 11180 0.0 79.097855 8 CACCGGC 11255 0.0 75.10932 9 CTTCACC 12490 0.0 74.03369 6 CCTTCAC 13750 0.0 70.7656 5 GTGTGGC 4450 0.0 69.088684 7 CGTAGTG 2520 0.0 66.49565 4 TTCACCG 13870 0.0 66.160065 7 AGCAGCC 50 6.1227183E-9 65.718 2 GCTGCCA 115 0.0 65.30981 2 AGGCAGC 30 1.3799935E-4 62.58857 2 ACCTTCA 5305 0.0 62.28922 4 TGTGGCA 5065 0.0 61.811886 8 CGGGCCC 700 0.0 61.019547 7 AGGCGCG 655 0.0 60.912052 5 GGGCCCG 695 0.0 60.78317 8 CAGCGTA 1230 0.0 59.85939 1 ATGCAGC 95 0.0 59.29443 2 GAGCTGC 45 7.952442E-6 58.854553 1 CGCTGCA 90 1.8189894E-12 58.854553 1 GCCTTCA 3940 0.0 58.613075 4 >>END_MODULE