FastQCFastQC Report
Mon 6 Mar 2017
1NF_10A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_10A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2149301
Sequences flagged as poor quality0
Sequence length96
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA126040.5864232138727893No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA54490.253524285337419No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG52010.24198565021837332No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG44850.20867249398758017No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA43450.2021587483558608No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT43170.2008559992295169No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG40600.18889862331986074No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG39790.18512995620436598No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT38120.17735998820081506No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC36730.170892769323608No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT34850.16214573947529917No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA33010.15358481664503948No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA32180.14972309602052017No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA30190.14046427187257626No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC28880.13436926703146745No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG28780.13390399948634463No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC28340.13185682228780427No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA27970.13013533237084987No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA26630.12390074726620422No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA24080.11203642486557258No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT23450.10910523933129887No Hit
CAGCTAAAAATATCAGATTAGTTCCGAAATTTCAGGAAAAAGCTATTGAT22510.10473172440714446No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC21710.10100958404616199No Hit
CAGCTCTGTTATAAAACAAGTTCTTGGATTTTTTTCATCATTTGAACATC21690.10091653053713742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGC3450.090.01
GCAGCTA202.2811322E-590.03
GCAGCCT355.0749804E-1090.03
GCTGCTA256.3772495E-789.999993
GCAGCTT158.205204E-489.999993
GCAGCAT158.205204E-489.999993
AGCAGCA1650.087.272732
CAGCAGC3600.086.250011
AGCTGCG1000.085.499992
AGCTGCA800.084.3752
CAGCCCT135050.083.902251
CAGCGTA48150.083.457951
AGCTGCC1350.083.3333362
AGCCCTT114900.082.28462
GCTGCCC1100.081.8181843
AGCTGCT550.081.8181842
GCAGCAC550.081.8181843
GCCCTTC103550.080.3524863
CAGCTAG30950.080.113081
GCTGCAA454.1836756E-1180.000013
Produced by FastQC (version 0.11.5)