##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename 1NF_10A_2.fq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2149301 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 13.790147122250444 12.0 12.0 12.0 2.0 32.0 2 14.541582123676488 12.0 12.0 12.0 12.0 22.0 3 13.737434635725755 12.0 12.0 12.0 12.0 22.0 4 13.772599556786137 12.0 12.0 12.0 12.0 22.0 5 22.40548531824998 22.0 22.0 22.0 22.0 27.0 6 26.740931121327353 27.0 22.0 32.0 22.0 32.0 7 34.859573414798575 37.0 37.0 37.0 27.0 41.0 8 37.46157704295489 41.0 37.0 41.0 27.0 41.0 9 38.44928979235575 41.0 37.0 41.0 32.0 41.0 10-11 38.820680072265354 41.0 41.0 41.0 34.5 41.0 12-13 38.999418880836146 41.0 41.0 41.0 37.0 41.0 14-15 38.94768485195884 41.0 41.0 41.0 37.0 41.0 16-17 38.83994796447776 41.0 41.0 41.0 37.0 41.0 18-19 38.77541931074335 41.0 41.0 41.0 34.5 41.0 20-21 38.801931418633316 41.0 41.0 41.0 37.0 41.0 22-23 38.79018248258387 41.0 41.0 41.0 34.5 41.0 24-25 38.81125235599853 41.0 41.0 41.0 34.5 41.0 26-27 38.774937526200375 41.0 41.0 41.0 32.0 41.0 28-29 38.72693401249988 41.0 41.0 41.0 32.0 41.0 30-31 38.59378211800022 41.0 41.0 41.0 32.0 41.0 32-33 38.63355667726391 41.0 41.0 41.0 32.0 41.0 34-35 38.61261451979039 41.0 41.0 41.0 32.0 41.0 36-37 38.53158771153971 41.0 41.0 41.0 32.0 41.0 38-39 38.430174042630604 41.0 41.0 41.0 32.0 41.0 40-41 38.476979027134874 41.0 41.0 41.0 32.0 41.0 42-43 38.2114226904468 41.0 41.0 41.0 32.0 41.0 44-45 38.33051047759248 41.0 41.0 41.0 32.0 41.0 46-47 38.33173738810898 41.0 41.0 41.0 32.0 41.0 48-49 38.28047723422638 41.0 41.0 41.0 32.0 41.0 50-51 38.157493529291614 41.0 41.0 41.0 32.0 41.0 52-53 38.13402101427394 41.0 37.0 41.0 32.0 41.0 54-55 37.994175548236385 41.0 37.0 41.0 32.0 41.0 56-57 37.785034297197086 41.0 37.0 41.0 27.0 41.0 58-59 37.78068381301642 41.0 37.0 41.0 27.0 41.0 60-61 37.77630029484004 41.0 37.0 41.0 27.0 41.0 62-63 37.76871480541813 41.0 37.0 41.0 27.0 41.0 64-65 37.772199891964874 41.0 37.0 41.0 27.0 41.0 66-67 37.631721429432176 41.0 37.0 41.0 27.0 41.0 68-69 37.64772872668836 41.0 37.0 41.0 27.0 41.0 70-71 37.6530730223454 41.0 37.0 41.0 27.0 41.0 72-73 37.45841089731034 41.0 37.0 41.0 27.0 41.0 74-75 37.40776745555881 41.0 37.0 41.0 27.0 41.0 76-77 36.34549185991166 39.0 34.5 41.0 24.5 41.0 78-79 36.87137748505212 41.0 37.0 41.0 27.0 41.0 80-81 37.53764572761098 41.0 37.0 41.0 27.0 41.0 82-83 37.722553053295 41.0 37.0 41.0 27.0 41.0 84-85 37.63849735332557 41.0 37.0 41.0 27.0 41.0 86-87 37.48787652357674 41.0 37.0 41.0 27.0 41.0 88-89 37.38425469489848 41.0 37.0 41.0 27.0 41.0 90-91 37.23439829972628 41.0 37.0 41.0 27.0 41.0 92-93 37.11878931801549 41.0 37.0 41.0 27.0 41.0 94-95 36.9542144166871 41.0 37.0 41.0 27.0 41.0 96-97 36.898257154302726 41.0 37.0 41.0 27.0 41.0 98-99 36.776444527779034 41.0 37.0 41.0 27.0 41.0 100 36.637586359472216 41.0 37.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2329664402524365 1101 2 -0.6824491074827232 1101 3 -0.0839452254229407 1101 4 -0.04214200714470273 1101 5 -1.1051274804328273 1101 6 -0.7191512909067157 1101 7 -0.31896415294480107 1101 8 -0.17099087540391622 1101 9 -0.27242501375351935 1101 10-11 -0.15140607096191872 1101 12-13 -0.2663488830970664 1101 14-15 -0.25873130400058386 1101 16-17 -0.0804479648757308 1101 18-19 -0.0712633515519201 1101 20-21 -0.17323221317954562 1101 22-23 -0.13352207221686285 1101 24-25 -0.16058289611517296 1101 26-27 -0.014642605712154477 1101 28-29 -0.12087343538070883 1101 30-31 -0.24350079131306757 1101 32-33 -0.22595058985326943 1101 34-35 -0.21615742832242546 1101 36-37 -0.2174093728943305 1101 38-39 -0.047981963929125016 1101 40-41 -0.1654947713514261 1101 42-43 -0.12259893360903362 1101 44-45 -0.046644753524901716 1101 46-47 -0.18352603991476713 1101 48-49 -0.20223907396253793 1101 50-51 -0.05549358060768128 1101 52-53 -0.18258184277406286 1101 54-55 -0.22808758370376836 1101 56-57 -0.28732318316485106 1101 58-59 -0.2665516908045049 1101 60-61 -0.12565616461175466 1101 62-63 -0.21776504213743664 1101 64-65 -0.10738163797470435 1101 66-67 -0.07036058401508427 1101 68-69 -0.13529363640785164 1101 70-71 -0.2480611078075583 1101 72-73 -0.10834334413254254 1101 74-75 -0.03356113933911331 1101 76-77 -0.4006133203026252 1101 78-79 -0.3155744015648523 1101 80-81 -0.2114335531686109 1101 82-83 -0.13354734966019777 1101 84-85 -0.0795355658567658 1101 86-87 -0.10332330028428771 1101 88-89 -0.3527805377235609 1101 90-91 -0.33141808731521394 1101 92-93 -0.3046013390378093 1101 94-95 -0.19065886391503284 1101 96-97 -0.33087169373536796 1101 98-99 -0.5280016508117669 1101 100 -0.6278185023669707 1102 1 1.5938343932383177 1102 2 0.7931862400470635 1102 3 0.01646600230222539 1102 4 0.11067749291015616 1102 5 -0.7762300179342674 1102 6 -0.09514009151335046 1102 7 -0.10539822109944197 1102 8 -0.336418859165903 1102 9 -0.17890146850110966 1102 10-11 -0.3362149493698965 1102 12-13 -0.23050526907060487 1102 14-15 -0.1959506768401127 1102 16-17 -0.2009510209601899 1102 18-19 -0.22258779370019965 1102 20-21 -0.09297794917441138 1102 22-23 -0.10952489984147462 1102 24-25 -0.17905147906692775 1102 26-27 -0.1635137085274181 1102 28-29 -0.08354087422777212 1102 30-31 -0.05022167726507121 1102 32-33 -0.142676211659861 1102 34-35 -0.14194478150746193 1102 36-37 -0.16799913261568378 1102 38-39 -0.14129760377641531 1102 40-41 -0.07312171933618572 1102 42-43 -0.12269517839578015 1102 44-45 -0.11456288018193561 1102 46-47 -0.33613694015767237 1102 48-49 -0.20493115036395437 1102 50-51 -0.15917088142055746 1102 52-53 -0.1666275680750502 1102 54-55 -0.25746335362734385 1102 56-57 -0.0716013958727828 1102 58-59 -0.0372764709900224 1102 60-61 -0.1330707629876713 1102 62-63 -0.39249821631272397 1102 64-65 -0.47566516150607185 1102 66-67 -0.3613787094221763 1102 68-69 -0.46407304974518127 1102 70-71 -0.38510271249841566 1102 72-73 -0.2881088846102031 1102 74-75 -0.4819396299486485 1102 76-77 -0.21383107656305356 1102 78-79 -0.3538506616007666 1102 80-81 -0.42719797704009466 1102 82-83 -0.22681340885976908 1102 84-85 -0.22902163536133457 1102 86-87 -0.16111808640003744 1102 88-89 -0.1832438876254443 1102 90-91 -0.39200244455142297 1102 92-93 -0.24347131691400392 1102 94-95 -0.14331531724604218 1102 96-97 -0.061970425887381 1102 98-99 -0.3440736969092697 1102 100 -0.65628501598637 1103 1 1.5133052560511313 1103 2 0.7152081127475451 1103 3 0.27540528885604587 1103 4 1.199583133234258 1103 5 2.729013691882173 1103 6 -0.33333047697506757 1103 7 -0.5831902955300166 1103 8 -0.3878520495130715 1103 9 -0.4111031667785028 1103 10-11 -0.45433083120334317 1103 12-13 -0.5566835499020328 1103 14-15 -0.4912571721354695 1103 16-17 -0.3833330573730507 1103 18-19 -0.6429400215919827 1103 20-21 -0.3896228409391256 1103 22-23 -0.4004599186087816 1103 24-25 -0.5045182166669875 1103 26-27 -0.35124093546183843 1103 28-29 -0.43321893442335835 1103 30-31 -0.3696764563434982 1103 32-33 -0.5987107639988665 1103 34-35 -0.4212039222825581 1103 36-37 -0.5164744476182719 1103 38-39 -0.42308477767574715 1103 40-41 -0.5310655523434278 1103 42-43 -0.1522357845227944 1103 44-45 -0.6170119675036503 1103 46-47 -0.7000031517665946 1103 48-49 -0.6765547002503425 1103 50-51 -0.3009525350051945 1103 52-53 -0.4658686868195545 1103 54-55 -0.43620769707221996 1103 56-57 -0.48612179717668624 1103 58-59 -0.7034724085804172 1103 60-61 -0.5097915900097618 1103 62-63 -0.44179780797438895 1103 64-65 -0.6337592861120029 1103 66-67 -0.6624501630323891 1103 68-69 -0.6000454116445297 1103 70-71 -0.6470158697590449 1103 72-73 -0.31541779894634914 1103 74-75 -0.5301984920568472 1103 76-77 -0.7937374845467104 1103 78-79 -0.8316378755621514 1103 80-81 -0.6298549451560049 1103 82-83 -0.7819471837004457 1103 84-85 -0.8256756000018584 1103 86-87 -0.6439743278610806 1103 88-89 -0.30908364414244716 1103 90-91 -0.5941690944273716 1103 92-93 -0.43342198757038375 1103 94-95 -0.5601207282149119 1103 96-97 -0.532020656627104 1103 98-99 -0.5453192142310925 1103 100 -0.3710013972993096 1104 1 1.8270268842418051 1104 2 -0.5887168082365033 1104 3 -0.012699691459278739 1104 4 0.0843203474367975 1104 5 -1.1105030631961057 1104 6 -0.5857581381029178 1104 7 -0.2948606280439279 1104 8 -0.024256800635932052 1104 9 -0.04755354845789128 1104 10-11 -0.24359881310822828 1104 12-13 0.03600164084524948 1104 14-15 -0.09690692815639323 1104 16-17 -0.12143705576501418 1104 18-19 -0.09030459703142668 1104 20-21 -0.02783808547269473 1104 22-23 0.00839535512834999 1104 24-25 -0.07076627592432772 1104 26-27 -0.08179654235979683 1104 28-29 -0.18855539912956232 1104 30-31 -0.02339396045601916 1104 32-33 -0.21719974566347133 1104 34-35 -0.26512315966310496 1104 36-37 -0.13056961101032272 1104 38-39 -0.12208916514040169 1104 40-41 -0.12234428226597771 1104 42-43 -0.13163503225397477 1104 44-45 -0.07729374485425922 1104 46-47 -0.3662112987270234 1104 48-49 -0.2466518991117752 1104 50-51 -0.014012000284388648 1104 52-53 -0.17364078356095547 1104 54-55 -0.1486263129530414 1104 56-57 -0.15667351103728322 1104 58-59 -0.061665057324482575 1104 60-61 -0.23029403442134821 1104 62-63 -0.013919212979658369 1104 64-65 -0.10856486528506082 1104 66-67 -0.7440243132643545 1104 68-69 -0.5473447180600601 1104 70-71 -0.30169970198645757 1104 72-73 -0.0800883116720783 1104 74-75 -0.4195097363501361 1104 76-77 -0.462902953651934 1104 78-79 -0.28707646029867107 1104 80-81 -0.34159863312155636 1104 82-83 -0.3537523516208978 1104 84-85 -0.16925578501933103 1104 86-87 -0.33714753452244395 1104 88-89 -0.23911582812485221 1104 90-91 -0.28048934238034917 1104 92-93 -0.15069736971185677 1104 94-95 -0.2786723219342946 1104 96-97 -0.3285560266620351 1104 98-99 -0.4688363366642321 1104 100 -0.3296329657890382 1105 1 1.4487433185339054 1105 2 0.21959413021971308 1105 3 -0.012581896622959832 1105 4 -0.2912733385382449 1105 5 -1.1856866568421687 1105 6 0.4729477595103653 1105 7 0.08459284826184899 1105 8 -0.21234743780344445 1105 9 -0.06724219314653368 1105 10-11 -0.05928966735084629 1105 12-13 -0.13455433415231255 1105 14-15 -0.15502579173833198 1105 16-17 -0.12538789457511257 1105 18-19 -0.07503464452629771 1105 20-21 -0.03394498553389269 1105 22-23 0.048599564256591066 1105 24-25 -0.11724645534942368 1105 26-27 -0.10727570403750519 1105 28-29 0.0560556949821418 1105 30-31 0.0012236359306427858 1105 32-33 -0.08312801886323484 1105 34-35 0.006058292057083747 1105 36-37 -0.09673297498694211 1105 38-39 0.15535523015356745 1105 40-41 0.029754489120016103 1105 42-43 0.13153415328203977 1105 44-45 0.027047535334475015 1105 46-47 -0.12804330499758976 1105 48-49 -0.13307300927682775 1105 50-51 -0.07251612227216953 1105 52-53 -0.14734315564407297 1105 54-55 -0.014657691280120844 1105 56-57 -0.024264770161529725 1105 58-59 -0.0066888213933467 1105 60-61 0.06348046690460762 1105 62-63 -0.23183619561365276 1105 64-65 -0.21537096777821318 1105 66-67 -0.17315256495616183 1105 68-69 -0.11361290558279791 1105 70-71 -0.2666576783321446 1105 72-73 -0.08534861331555277 1105 74-75 -0.059081096184812054 1105 76-77 -0.3218311647668912 1105 78-79 -0.22228403420177045 1105 80-81 -0.35324923534419383 1105 82-83 -0.28847128482651385 1105 84-85 -0.05046398863976265 1105 86-87 -0.1251190464649028 1105 88-89 -0.20382421799970274 1105 90-91 -0.19195571347898976 1105 92-93 -0.11900085799112503 1105 94-95 -0.16917897276933758 1105 96-97 -0.05341847000965316 1105 98-99 -0.13863593023047116 1105 100 -0.34085451764315877 1106 1 1.3539435479302337 1106 2 -0.7087658598166069 1106 3 0.01599609716325645 1106 4 -0.26451043354655823 1106 5 -1.1941152340635774 1106 6 -0.6761598540147489 1106 7 -0.2131705375352766 1106 8 -0.27343676141253326 1106 9 -0.10837326379790113 1106 10-11 -0.06753287380174555 1106 12-13 -0.11195261676935786 1106 14-15 -0.15091867060432662 1106 16-17 -0.15295021101459838 1106 18-19 0.0615169957153725 1106 20-21 -0.16827796936608763 1106 22-23 -0.20647544332236833 1106 24-25 -0.5001722626294764 1106 26-27 -0.4287337223853456 1106 28-29 -0.27380290187698364 1106 30-31 -0.04056299072683345 1106 32-33 -0.1721996395637433 1106 34-35 -0.09698468499749424 1106 36-37 -0.42153346004045744 1106 38-39 -0.2355115906025489 1106 40-41 -0.21700297060528584 1106 42-43 0.04858652631754268 1106 44-45 -0.11139643152277046 1106 46-47 -0.3119436604846584 1106 48-49 -0.22934898773520018 1106 50-51 -0.4082090710383852 1106 52-53 -0.16362971568393192 1106 54-55 -0.1599227232083038 1106 56-57 -0.1437400936431814 1106 58-59 -0.38203277106517675 1106 60-61 -0.23885153600726028 1106 62-63 -0.3980143330622141 1106 64-65 -0.4663485089508015 1106 66-67 -0.4674566618432934 1106 68-69 -0.5302868132951843 1106 70-71 -0.7647721100259162 1106 72-73 -0.5037314078185418 1106 74-75 -0.6469738746416382 1106 76-77 -0.5626876549644493 1106 78-79 -0.3242205794698876 1106 80-81 -0.3060018947389196 1106 82-83 -0.36088560394615143 1106 84-85 -0.6163324024259396 1106 86-87 -0.43904348084268463 1106 88-89 -0.4598309067950126 1106 90-91 -0.47976990245290096 1106 92-93 -0.4314461880959257 1106 94-95 -0.2778661035966934 1106 96-97 -0.25210600633556624 1106 98-99 -0.4646393943467473 1106 100 -0.7215790470234609 1107 1 1.0921805192487337 1107 2 -0.6408167975766599 1107 3 -0.20902404387373252 1107 4 -0.04785061870042995 1107 5 -1.1895413675701931 1107 6 -0.5340367953178529 1107 7 -0.5564887416068061 1107 8 -0.40224932986166806 1107 9 -0.26112797193797377 1107 10-11 -0.23344031454730896 1107 12-13 -0.379813903872801 1107 14-15 -0.39988302345540916 1107 16-17 -0.47754636802738304 1107 18-19 -0.3257977702894266 1107 20-21 -0.23452858133312304 1107 22-23 -0.24438231160938528 1107 24-25 -0.11433505857010573 1107 26-27 -0.32479657789261296 1107 28-29 -0.388752828330432 1107 30-31 -0.4436066909751588 1107 32-33 -0.3063388077748357 1107 34-35 -0.31452487589805145 1107 36-37 -0.22684077524299084 1107 38-39 -0.28058828126164315 1107 40-41 -0.3038520062115495 1107 42-43 -0.2529018659248692 1107 44-45 -0.017478693154018288 1107 46-47 -0.4081450167030738 1107 48-49 -0.3320250380806087 1107 50-51 -0.2186088360872489 1107 52-53 -0.3575553004923364 1107 54-55 -0.38343214832951134 1107 56-57 -0.21649800114139595 1107 58-59 -0.5152086782959486 1107 60-61 -0.20960172194577353 1107 62-63 -0.3539243874457796 1107 64-65 -0.4965110241878108 1107 66-67 -0.4463026329175577 1107 68-69 -0.5045904141479056 1107 70-71 -0.49512313321270085 1107 72-73 -0.27007893989223675 1107 74-75 -0.4123754134279167 1107 76-77 -0.5372292736959992 1107 78-79 -0.8093655286282697 1107 80-81 -0.5435238454301228 1107 82-83 -0.5449144523208957 1107 84-85 -0.512348427950073 1107 86-87 -0.5522321026210903 1107 88-89 -0.4941781437983721 1107 90-91 -0.3512268481544325 1107 92-93 -0.387368122652191 1107 94-95 -0.3175968546777881 1107 96-97 -0.455418723424998 1107 98-99 -0.4052309330114596 1107 100 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32-33 0.20181290629069082 2223 34-35 0.20492226319428397 2223 36-37 0.3085546373884398 2223 38-39 0.20214657077524123 2223 40-41 0.20622759368713872 2223 42-43 0.24552445970716263 2223 44-45 0.2885737554725267 2223 46-47 0.34269503977674987 2223 48-49 0.22089717282408827 2223 50-51 0.26352955563910285 2223 52-53 0.284770342315916 2223 54-55 0.38168105382835904 2223 56-57 0.544810492927688 2223 58-59 0.35401386189472106 2223 60-61 0.40904820556819743 2223 62-63 0.19945860824817174 2223 64-65 0.34017620264226167 2223 66-67 0.5419787476044533 2223 68-69 0.4557089342862355 2223 70-71 0.5622216685420582 2223 72-73 0.5800885324741429 2223 74-75 0.45152883495394036 2223 76-77 0.54745865913182 2223 78-79 0.5947083747847657 2223 80-81 0.4394661897619798 2223 82-83 0.34717244468427566 2223 84-85 0.4636520948116214 2223 86-87 0.3884504786360168 2223 88-89 0.6377078941530456 2223 90-91 0.5796358972606228 2223 92-93 0.6491261673889284 2223 94-95 0.7836341952109223 2223 96-97 0.6611591545936761 2223 98-99 0.7123582463604734 2223 100 0.9377341968618254 2224 1 1.2310256868433864 2224 2 -0.8176585024448073 2224 3 0.035850727409245664 2224 4 -0.39314227252396705 2224 5 -1.1202567791078053 2224 6 0.5612838238631142 2224 7 0.40598417851657587 2224 8 0.13772723552461485 2224 9 0.4424099551917422 2224 10-11 0.2828994261416895 2224 12-13 0.1459786538884913 2224 14-15 0.2574373967936765 2224 16-17 0.3152496743210591 2224 18-19 0.24260392128744712 2224 20-21 0.24325253019333815 2224 22-23 0.22726934772303764 2224 24-25 0.2710252486157074 2224 26-27 0.2983045516470284 2224 28-29 0.37389765489917437 2224 30-31 0.21935951465046344 2224 32-33 0.22043529304210097 2224 34-35 0.32135352862447775 2224 36-37 0.21613108135991155 2224 38-39 0.16786158025115583 2224 40-41 0.13588037574643153 2224 42-43 0.45461852459372665 2224 44-45 0.40664300069636994 2224 46-47 0.4343547592252932 2224 48-49 0.31560674336538597 2224 50-51 0.11617249185962208 2224 52-53 0.20130011974580952 2224 54-55 0.24383234145391697 2224 56-57 0.4089064208303128 2224 58-59 0.6144080171174622 2224 60-61 0.3639298437356331 2224 62-63 0.4740396976970729 2224 64-65 0.3298856525566123 2224 66-67 0.380756421434576 2224 68-69 0.4098308908015653 2224 70-71 0.5161689840565629 2224 72-73 0.5207963713487231 2224 74-75 0.3720275067173233 2224 76-77 0.6671951026743983 2224 78-79 0.3659259831010573 2224 80-81 0.3359769885535613 2224 82-83 0.36386707892999226 2224 84-85 0.30979471338119424 2224 86-87 0.37305995562442007 2224 88-89 0.38419577382633463 2224 90-91 0.38924436902453863 2224 92-93 0.3476854796545439 2224 94-95 0.5983339279552382 2224 96-97 0.3971577177928154 2224 98-99 0.4329043647196258 2224 100 0.5622305793939688 2225 1 1.736407123250311 2225 2 1.7236992579099546 2225 3 0.772198413939698 2225 4 1.1822272675361578 2225 5 3.1958247813386507 2225 6 0.30076246902959625 2225 7 0.2556050128376768 2225 8 0.5994350929995775 2225 9 0.29469140234480307 2225 10-11 0.3362029439071037 2225 12-13 0.23159536039865714 2225 14-15 0.20208657876458602 2225 16-17 0.3461928661600453 2225 18-19 0.17684190978422265 2225 20-21 0.21761206879004646 2225 22-23 0.2909072430146651 2225 24-25 0.36064083947209724 2225 26-27 0.21476754580037039 2225 28-29 0.10350835329519015 2225 30-31 0.1916647328968537 2225 32-33 0.08478561912512816 2225 34-35 0.26153105041510116 2225 36-37 0.13378897525831945 2225 38-39 0.12637273327878518 2225 40-41 0.19373236964003127 2225 42-43 0.5188254519072117 2225 44-45 0.4284148543610442 2225 46-47 0.29568901308998363 2225 48-49 0.11956110416219445 2225 50-51 0.28237736282772374 2225 52-53 0.31928820071873076 2225 54-55 0.374938939309331 2225 56-57 0.16874750226924817 2225 58-59 0.3467753145299213 2225 60-61 0.19869819977714798 2225 62-63 0.26797139981427165 2225 64-65 0.30427421651149444 2225 66-67 0.39682958477740726 2225 68-69 0.5554189823586171 2225 70-71 0.39064281506363585 2225 72-73 0.4025349120023165 2225 74-75 0.40254692832699135 2225 76-77 0.5473371136268099 2225 78-79 0.4119348208501137 2225 80-81 0.3230634041999352 2225 82-83 0.22393528717168465 2225 84-85 0.21494429792706882 2225 86-87 0.2761642649315945 2225 88-89 0.30789782730967374 2225 90-91 0.43065545503733915 2225 92-93 0.4135202570556942 2225 94-95 0.5162390099039555 2225 96-97 0.5607416664187426 2225 98-99 0.4588550599511976 2225 100 0.6149303697093842 2226 1 1.431406405335844 2226 2 1.6309753613606244 2226 3 0.67747941426612 2226 4 0.7407865019328099 2226 5 3.212949864624793 2226 6 -0.4503997726489146 2226 7 0.10843416048767551 2226 8 0.15663701195536817 2226 9 0.07277355434584365 2226 10-11 0.17663825282274104 2226 12-13 0.12233886165944341 2226 14-15 0.045876591740814376 2226 16-17 0.18488824080115762 2226 18-19 0.07827803448731174 2226 20-21 0.022684040387517257 2226 22-23 0.11015049663529197 2226 24-25 0.11340010473638529 2226 26-27 0.06907379574323613 2226 28-29 0.09446343766224174 2226 30-31 0.12826481904659204 2226 32-33 0.04222552538216462 2226 34-35 0.0018336930394298179 2226 36-37 -0.00575248981419918 2226 38-39 0.0750858110991004 2226 40-41 0.14273785644250125 2226 42-43 0.23175202619683688 2226 44-45 0.22059905131590085 2226 46-47 0.2126474165342529 2226 48-49 0.2451732808519722 2226 50-51 0.04312483816185164 2226 52-53 0.014091871278260726 2226 54-55 0.10741308766649382 2226 56-57 0.1306703407148362 2226 58-59 0.18322781144718903 2226 60-61 0.19209110118947592 2226 62-63 0.08032442228795134 2226 64-65 0.044273963762584856 2226 66-67 0.1331047810208119 2226 68-69 0.3899828533534162 2226 70-71 0.47662396214575153 2226 72-73 0.1913010832273372 2226 74-75 0.23475472235060835 2226 76-77 0.5278043280595242 2226 78-79 0.4255651126460478 2226 80-81 0.4495647730772916 2226 82-83 0.1014393163657914 2226 84-85 0.23763442829711323 2226 86-87 0.16260606035233138 2226 88-89 0.30940343107574364 2226 90-91 0.3448805403132127 2226 92-93 0.04284945606212176 2226 94-95 0.5773976593213064 2226 96-97 0.6195215183508935 2226 98-99 0.3153185487573822 2226 100 0.601495151675806 2227 1 1.0929567731465202 2227 2 1.5769736204773555 2227 3 0.6424108697143431 2227 4 -0.10199570440767047 2227 5 3.162793119683826 2227 6 0.0740882156928393 2227 7 0.30653162377205234 2227 8 0.36777691963183656 2227 9 0.1341691406177361 2227 10-11 0.19684547901540128 2227 12-13 0.08783943104607772 2227 14-15 0.27874290804927426 2227 16-17 0.07569253759776018 2227 18-19 0.033562640541575206 2227 20-21 0.013762559022389098 2227 22-23 0.10443536011339916 2227 24-25 0.12581807815628565 2227 26-27 0.1493404039441515 2227 28-29 -0.04694896842168106 2227 30-31 0.12996070768241452 2227 32-33 0.15939591378675289 2227 34-35 0.09180605269980191 2227 36-37 0.2264953548065165 2227 38-39 0.18319809806996545 2227 40-41 0.17179784492387995 2227 42-43 0.4439418086518785 2227 44-45 0.2778016831474517 2227 46-47 0.15521326686192793 2227 48-49 0.06043131553001757 2227 50-51 0.13262843555096282 2227 52-53 0.23595631799152983 2227 54-55 0.21602304255187477 2227 56-57 0.3038222338708536 2227 58-59 0.2775739221656224 2227 60-61 0.2700130799498268 2227 62-63 0.06261235303657742 2227 64-65 -0.0209393489768388 2227 66-67 0.4621428993834158 2227 68-69 0.3512162953283422 2227 70-71 0.4111461355010775 2227 72-73 0.2357721426791528 2227 74-75 0.15422441832868827 2227 76-77 0.16059930742198958 2227 78-79 0.021052723578272037 2227 80-81 0.07115417986804573 2227 82-83 0.133700468490197 2227 84-85 0.09424389451350379 2227 86-87 0.14824895458166765 2227 88-89 0.12452539107469107 2227 90-91 0.14799084206630653 2227 92-93 0.05163758969563759 2227 94-95 0.41612053164781315 2227 96-97 0.19122169374816167 2227 98-99 0.44581034828093635 2227 100 0.5178024066650266 2228 1 1.4815434481863363 2228 2 1.8708363504691992 2228 3 -0.06253597805403999 2228 4 0.19747520105775251 2228 5 3.153428626583043 2228 6 0.21455965850725178 2228 7 0.1750931469945911 2228 8 0.17028929592287767 2228 9 0.07308076073574199 2228 10-11 -0.010572041151370115 2228 12-13 0.10810776565264746 2228 14-15 0.05568359572649939 2228 16-17 0.13630723031614167 2228 18-19 0.12423219033375688 2228 20-21 0.17204474709022577 2228 22-23 0.1842508379223915 2228 24-25 0.15693981036226745 2228 26-27 0.08140325219593336 2228 28-29 0.14123081042360042 2228 30-31 0.2546058469399668 2228 32-33 0.11519464313981587 2228 34-35 0.04641382030607133 2228 36-37 0.14925993444422403 2228 38-39 0.01429854672399955 2228 40-41 0.13491849379975918 2228 42-43 0.14516902527041964 2228 44-45 0.04706424179185831 2228 46-47 0.007657152736769035 2228 48-49 0.06682717125288917 2228 50-51 0.09309187747626879 2228 52-53 0.09842842547859476 2228 54-55 0.1329848195585157 2228 56-57 0.3182478423268691 2228 58-59 0.42837571052348977 2228 60-61 0.10533449665210526 2228 62-63 0.24753270022013396 2228 64-65 0.2134850833538735 2228 66-67 0.26318228663292587 2228 68-69 0.18958228502160068 2228 70-71 0.2019838496314179 2228 72-73 0.15429471349484203 2228 74-75 -0.05983780517396298 2228 76-77 0.25431423946568543 2228 78-79 0.30278935891036696 2228 80-81 0.18117679060726033 2228 82-83 0.12422659034109529 2228 84-85 0.16386889397399784 2228 86-87 0.13075179778365964 2228 88-89 0.2486377671499369 2228 90-91 0.26165080913479954 2228 92-93 0.3193848079974444 2228 94-95 0.6734896580348888 2228 96-97 0.3383112691777157 2228 98-99 0.45661788778362933 2228 100 0.4430782566043874 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 22.0 13 18.0 14 119.0 15 412.0 16 1434.0 17 3779.0 18 6239.0 19 7979.0 20 9272.0 21 10684.0 22 12297.0 23 13893.0 24 15913.0 25 17877.0 26 20118.0 27 22524.0 28 25515.0 29 28550.0 30 32816.0 31 38204.0 32 44918.0 33 53032.0 34 64977.0 35 83602.0 36 118037.0 37 193939.0 38 474214.0 39 848005.0 40 912.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.846030906806522 19.981424203873825 19.152601372760785 35.01994351655887 2 20.8338083217979 52.052764432863775 10.053206462945083 17.060220782393245 3 40.40001842459478 14.969285363008716 24.074385114044055 20.55631109835244 4 17.925548817964536 16.277478119630523 22.32949223956998 43.46748082283496 5 16.99385055885611 34.619860131270585 26.8898120830912 21.4964772267821 6 17.209874280056635 27.66843731985422 30.95629695421907 24.16539144587008 7 20.101325965976848 26.934570820932013 36.52094332064239 16.443159892448755 8 20.171674418799416 27.681790498399245 34.91702651234052 17.229508570460812 9 20.51062182542138 28.07061458585838 28.000359186544838 23.418404402175405 10-11 19.23967373578666 30.323440039343026 28.87154940141004 21.565336823460278 12-13 23.94927467116053 26.645476831769955 27.442945404110453 21.96230309295906 14-15 21.910076811019024 27.409353087352585 28.520970306160002 22.159599795468388 16-17 20.935062143459664 29.913795229239646 28.25395326201402 20.897189365286668 18-19 21.477982841863472 29.673065801393108 29.794849581328997 19.054101775414427 20-21 22.69181934033437 29.193886756671123 29.795152005233327 18.319141897761178 22-23 22.004409805792672 30.39434681321974 28.6738339581101 18.927409422877485 24-25 22.245813871579646 28.276728108347783 26.38453152908783 23.092926490984745 26-27 19.958116615588047 26.414308651975688 30.150616409707155 23.47695832272911 28-29 19.172355554211443 28.270116794247407 30.759610429943805 21.79791722159734 30-31 19.937159448883016 30.315598695261713 28.273408745096763 21.47383311075851 32-33 20.368296483368315 30.256953307144975 28.931452597844604 20.443297611642112 34-35 21.37841558720719 29.258047151143558 30.008291067654085 19.355246193995164 36-37 21.997477319370347 28.19184004474013 28.681161921945787 21.12952071394374 38-39 21.539053507171232 29.15399189124554 28.991332919551738 20.315621682031487 40-41 20.688224053644237 30.004894682894808 29.879014791526988 19.42786647193397 42-43 20.388089325868574 30.184888189125854 29.461163849236428 19.965858635769152 44-45 20.625845332971046 30.0397896804589 28.75876854847227 20.575596438097783 46-47 19.83583587123238 28.059047533525046 28.950032139095867 23.15508445614671 48-49 20.74854121143135 28.01932535724995 30.442136871973 20.789996559345703 50-51 21.916404449632694 30.667016858038963 28.252092191833533 19.164486500494814 52-53 20.45326542191874 30.426672678008654 28.144758884468008 20.975303015604595 54-55 22.345417981012 28.3144014089177 28.308351755690804 21.031828854379494 56-57 21.611177121622692 28.740824222215345 29.364024459899717 20.283974196262243 58-59 21.146472807479817 28.1117459076563 28.974526316524212 21.767254968339667 60-61 19.563847883213853 30.426916534530374 29.783473286136484 20.22576229611929 62-63 20.315498793299245 31.458024426238396 27.243029043413074 20.983447737049286 64-65 19.802065650722785 30.283625099073024 28.91147972233731 21.002829527866883 66-67 20.19896236032087 30.35559002671101 29.45006306701574 19.995384545952383 68-69 20.917591347140302 30.043767717969704 29.27823976260189 19.760401172288105 70-71 19.56443048228238 31.05346808101797 29.46243918371601 19.919662252983645 72-73 20.191192392317316 30.796361235583102 28.555911898798726 20.456534473300856 74-75 19.943414161162167 29.066263869043933 30.733666433877804 20.256655535916096 76-77 19.45111457166772 30.3979759000717 28.699679570241678 21.451229958018907 78-79 19.363714063316397 29.540045810242493 28.526064985779097 22.57017514066201 80-81 20.614657078410794 29.51003491375781 28.507799763271326 21.367508244560078 82-83 19.70059102936257 28.601857068879603 30.25223084156198 21.445321060195848 84-85 20.35999145768787 29.231271003921737 28.49270530279379 21.916032235596596 86-87 21.215269522509878 28.80362033982211 29.435616509739678 20.545493627928334 88-89 21.75260701037221 28.039371870203382 29.034974626634423 21.173046492789982 90-91 21.550820476052447 27.887624860361576 28.60688195836693 21.954672705219043 92-93 21.092555207483734 28.009408640297472 29.89171828422357 21.00631786799522 94-95 21.38727893394178 28.413307396218585 29.12970309882143 21.069710571018206 96-97 20.612399100916996 28.324929826022505 29.71635894646678 21.346312126593716 98-99 22.319279616954535 28.51617805044524 28.350705648022313 20.813836684577915 100 21.85175552423788 30.301898152003837 27.733062981871782 20.1132833418865 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 5.5 2 10.5 3 13.5 4 19.0 5 24.0 6 30.0 7 33.0 8 42.0 9 49.0 10 62.0 11 76.0 12 87.5 13 97.5 14 106.0 15 136.0 16 184.5 17 273.5 18 443.0 19 677.0 20 1061.0 21 1594.0 22 2338.0 23 3630.5 24 5516.5 25 8205.0 26 12054.5 27 16524.5 28 21121.0 29 26369.0 30 32376.0 31 38976.5 32 46562.0 33 55137.5 34 64542.5 35 74841.0 36 85034.5 37 92460.0 38 97440.0 39 100852.0 40 102502.0 41 102658.5 42 101201.5 43 99661.5 44 99374.5 45 97620.0 46 90319.5 47 81436.5 48 75846.0 49 73649.0 50 71434.5 51 66085.0 52 58152.0 53 49093.5 54 40835.0 55 33567.5 56 26555.5 57 19757.5 58 14033.0 59 9964.0 60 7618.5 61 6358.5 62 5322.0 63 4308.0 64 3441.5 65 2996.0 66 2891.5 67 2433.5 68 1781.5 69 1768.5 70 2178.0 71 2134.5 72 1368.5 73 606.0 74 287.5 75 166.0 76 116.0 77 96.5 78 72.0 79 52.5 80 47.0 81 41.5 82 32.0 83 21.5 84 17.0 85 13.5 86 11.0 87 11.0 88 12.5 89 15.5 90 15.5 91 14.5 92 13.5 93 9.0 94 10.0 95 18.0 96 20.0 97 14.5 98 37.5 99 78.0 100 73.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 11.38379408002881 2 0.005862371068547402 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 3.9547741335438824E-4 30-31 0.009817145202091284 32-33 0.0 34-35 0.0 36-37 0.0 38-39 8.840083357333384E-4 40-41 0.0013958026353684291 42-43 9.305350902456194E-5 44-45 0.0 46-47 0.002303074348357908 48-49 2.3263377256140485E-5 50-51 0.0 52-53 0.0014190660126245694 54-55 0.019424920008877303 56-57 0.010817470424105325 58-59 0.014818771312161489 60-61 2.3263377256140484E-4 62-63 0.01207369279593691 64-65 1.1631688628070242E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.3263377256140484E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2149301.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.0403806182124 #Duplication Level Percentage of deduplicated Percentage of total 1 87.1847839786544 66.29564157853505 2 8.011189070928676 12.183477323157597 3 2.1913844465581525 4.9990112219133795 4 0.9270499462849981 2.8197292307041844 5 0.49908367985413443 1.8975256488223224 6 0.2972172839530347 1.3560309238860044 7 0.19952480664943006 1.062035956827853 8 0.13851573746125367 0.8426231518532887 9 0.08568932373241218 0.5864263912376859 >10 0.4382416380853909 5.695558791158814 >50 0.019021639061975647 0.9944652728495899 >100 0.00804919715345836 1.142004402762552 >500 2.492516227232506E-4 0.12547010629167094 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.652675451228097E-5 0.0 2 0.0 0.0 0.0 1.8610701804912388E-4 0.0 3 0.0 0.0 0.0 1.8610701804912388E-4 0.0 4 0.0 0.0 0.0 1.8610701804912388E-4 0.0 5 0.0 0.0 0.0 1.8610701804912388E-4 0.0 6 0.0 0.0 0.0 1.8610701804912388E-4 0.0 7 0.0 0.0 0.0 1.8610701804912388E-4 0.0 8 0.0 0.0 4.652675451228097E-5 1.8610701804912388E-4 0.0 9 0.0 0.0 4.652675451228097E-5 1.8610701804912388E-4 0.0 10-11 0.0 0.0 4.652675451228097E-5 1.8610701804912388E-4 0.0 12-13 9.305350902456194E-5 0.0 4.652675451228097E-5 1.8610701804912388E-4 0.0 14-15 9.305350902456194E-5 0.0 4.652675451228097E-5 1.8610701804912388E-4 0.0 16-17 9.305350902456194E-5 0.0 4.652675451228097E-5 1.8610701804912388E-4 0.0 18-19 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 20-21 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 22-23 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 24-25 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 26-27 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 28-29 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 30-31 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 32-33 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 34-35 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 36-37 9.305350902456194E-5 0.0 9.305350902456194E-5 1.8610701804912388E-4 0.0 38-39 9.305350902456194E-5 0.0 9.305350902456194E-5 2.3263377256140484E-4 0.0 40-41 9.305350902456194E-5 0.0 9.305350902456194E-5 2.3263377256140484E-4 0.0 42-43 9.305350902456194E-5 0.0 9.305350902456194E-5 2.3263377256140484E-4 0.0 44-45 9.305350902456194E-5 0.0 9.305350902456194E-5 2.3263377256140484E-4 0.0 46-47 9.305350902456194E-5 0.0 9.305350902456194E-5 2.3263377256140484E-4 0.0 48-49 1.3958026353684292E-4 0.0 9.305350902456194E-5 2.3263377256140484E-4 0.0 50-51 1.3958026353684292E-4 0.0 9.305350902456194E-5 2.3263377256140484E-4 0.0 52-53 1.8610701804912388E-4 0.0 9.305350902456194E-5 2.3263377256140484E-4 0.0 54-55 2.5589714981754535E-4 0.0 9.305350902456194E-5 2.3263377256140484E-4 0.0 56-57 2.7916052707368583E-4 0.0 1.628436407929834E-4 2.3263377256140484E-4 0.0 58-59 3.024239043298263E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 60-61 3.9547741335438824E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 62-63 4.187407906105287E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 64-65 4.187407906105287E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 66-67 5.583210541473717E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 68-69 5.583210541473717E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 70-71 5.815844314035121E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 72-73 6.048478086596526E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 74-75 6.74637940428074E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 76-77 8.374815812210574E-4 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 78-79 0.0011166421082947433 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 80-81 0.0014190660126245694 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 82-83 0.0015586462761614124 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 84-85 0.0017447532942105365 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 86-87 0.0017680166714666768 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 88 0.0017680166714666768 0.0 2.3263377256140484E-4 2.3263377256140484E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCACCGG 8810 0.0 80.18294 8 CACCGGC 8705 0.0 77.21393 9 CTTCACC 9975 0.0 73.547424 6 AGGCGCG 300 0.0 71.97107 5 CCTTCAC 10815 0.0 70.829636 5 CGGCTGC 30 7.5730815E-5 70.65385 1 CGGGGCA 865 0.0 69.99951 94 CGTAGTG 1555 0.0 69.12362 4 CGGGCCC 575 0.0 67.75349 7 GTGTGGC 3640 0.0 67.69854 7 TTCACCG 10840 0.0 67.54865 7 GGCGCGC 340 0.0 63.503887 6 ATGCTGC 45 2.2226232E-7 62.590813 2 GGGCCCG 625 0.0 62.333202 8 CAGCGTA 855 0.0 61.97706 1 AACGGGC 630 0.0 61.093452 5 TGTGGCA 3950 0.0 60.959534 8 ACCTTCA 4355 0.0 59.924995 4 GCCTTCA 3280 0.0 59.530685 4 TGTGTGG 3940 0.0 58.969894 6 >>END_MODULE