FastQCFastQC Report
Tue 7 Mar 2017
1NF_10A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_10A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2149301
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG88100.080.182948
CACCGGC87050.077.213939
CTTCACC99750.073.5474246
AGGCGCG3000.071.971075
CCTTCAC108150.070.8296365
CGGCTGC307.5730815E-570.653851
CGGGGCA8650.069.9995194
CGTAGTG15550.069.123624
CGGGCCC5750.067.753497
GTGTGGC36400.067.698547
TTCACCG108400.067.548657
GGCGCGC3400.063.5038876
ATGCTGC452.2226232E-762.5908132
GGGCCCG6250.062.3332028
CAGCGTA8550.061.977061
AACGGGC6300.061.0934525
TGTGGCA39500.060.9595348
ACCTTCA43550.059.9249954
GCCTTCA32800.059.5306854
TGTGTGG39400.058.9698946
Produced by FastQC (version 0.11.5)