FastQCFastQC Report
Mon 6 Mar 2017
1NF_11A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_11A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2266647
Sequences flagged as poor quality0
Sequence length96
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA142690.6295201678955743No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA61540.2715023556822037No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG55210.2435756427886654No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT53460.2358549875653333No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA51330.22645784720779194No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG48900.21573716595482223No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG45320.1999429112693772No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG41040.1810603944945993No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT40570.17898684709176152No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT37290.16451613330174483No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA37220.16420730709281153No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC36030.15895726154094572No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA33880.14947188512370915No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA33290.14686892136270008No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA28470.1256040309761511No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA27510.12136870011078037No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC27010.11916279861839978No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC25870.11413334321577202No Hit
CAGCACTTTGAACTTTGTTCACCATTTCTTCGTAGTCGTTGATACGTTGT25340.11179508763384859No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCAGC3000.089.999851
GCTGCCG158.205319E-489.999853
GCTGCCC850.089.999853
GCTGCAC202.2811786E-589.999853
CTGCAGC401.4551915E-1189.999851
AGCTGCC1350.089.999852
GCAGCTA202.2811786E-589.999853
GCAGCCT158.205319E-489.999853
AGCAGCC550.089.999852
AGCAGCA1050.089.999852
AGCAGCG700.089.999842
CAGCTGC3250.085.846011
AGCAGCT750.083.9998552
CAGCCCT151500.083.37611
CAGCGTA46050.083.0617451
CTGCTGC1100.081.818041
GCTGCTA550.081.818043
GCCCTTC115550.081.7826463
AGCCCTT128500.081.630212
CAGCTAG36300.080.3304441
Produced by FastQC (version 0.11.5)