FastQCFastQC Report
Tue 7 Mar 2017
1NF_11A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_11A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2266647
Sequences flagged as poor quality0
Sequence length100
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG94450.080.05998
CACCGGC91200.078.58979
CTTCACC103000.076.193956
GTGTGGC43850.072.367
CCTTCAC113350.072.300975
TGTGGCA47300.067.280628
CGTAGTG16500.067.135084
GAGCAGC403.9482893E-666.228151
CGGGCCC6150.065.6364067
TTCACCG120150.064.771247
CAGCGTA8800.064.4219361
TAGCTGC250.003258322663.5790251
ACGGGCC6350.062.0907676
AACGGGC6500.061.3800165
ACCTTCA44800.060.8722764
TGTGTGG49900.059.7302866
GTGGCAC48350.059.606469
GCCTTCA33450.059.2158244
GCGTAGT12700.057.6557123
ATGTGTG48750.057.2880135
Produced by FastQC (version 0.11.5)