##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_11A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2266647
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.84571086719723	12.0	12.0	12.0	2.0	32.0
2	14.611820455501011	12.0	12.0	12.0	12.0	22.0
3	13.740954811225569	12.0	12.0	12.0	12.0	22.0
4	13.75646009281551	12.0	12.0	12.0	12.0	22.0
5	22.42446397696686	22.0	22.0	22.0	22.0	27.0
6	26.792836731965764	27.0	22.0	32.0	22.0	32.0
7	34.920864166321444	37.0	37.0	37.0	27.0	41.0
8	37.522901007523444	41.0	37.0	41.0	27.0	41.0
9	38.49417928773205	41.0	37.0	41.0	32.0	41.0
10-11	38.85935966209119	41.0	41.0	41.0	37.0	41.0
12-13	39.03306800750183	41.0	41.0	41.0	37.0	41.0
14-15	38.986179585969936	41.0	41.0	41.0	37.0	41.0
16-17	38.885970334154365	41.0	41.0	41.0	37.0	41.0
18-19	38.82363905804477	41.0	41.0	41.0	37.0	41.0
20-21	38.84306599130787	41.0	41.0	41.0	37.0	41.0
22-23	38.831218314982436	41.0	41.0	41.0	34.5	41.0
24-25	38.84915913241012	41.0	41.0	41.0	37.0	41.0
26-27	38.81068026913763	41.0	41.0	41.0	37.0	41.0
28-29	38.76144984199128	41.0	41.0	41.0	34.5	41.0
30-31	38.64141990349622	41.0	41.0	41.0	32.0	41.0
32-33	38.68705096117746	41.0	41.0	41.0	32.0	41.0
34-35	38.65793460560908	41.0	41.0	41.0	32.0	41.0
36-37	38.57345828441747	41.0	41.0	41.0	32.0	41.0
38-39	38.475603611854865	41.0	41.0	41.0	32.0	41.0
40-41	38.51789691998798	41.0	41.0	41.0	32.0	41.0
42-43	38.25688539062324	41.0	41.0	41.0	32.0	41.0
44-45	38.37954630782826	41.0	41.0	41.0	32.0	41.0
46-47	38.3781307808406	41.0	41.0	41.0	32.0	41.0
48-49	38.338254258382534	41.0	41.0	41.0	32.0	41.0
50-51	38.21728901765471	41.0	41.0	41.0	32.0	41.0
52-53	38.194789925383176	41.0	41.0	41.0	32.0	41.0
54-55	38.052952444734444	41.0	37.0	41.0	32.0	41.0
56-57	37.83811131596583	41.0	37.0	41.0	27.0	41.0
58-59	37.83348929056885	41.0	37.0	41.0	27.0	41.0
60-61	37.83439393077087	41.0	37.0	41.0	27.0	41.0
62-63	37.82771931403522	41.0	37.0	41.0	27.0	41.0
64-65	37.83152493528988	41.0	37.0	41.0	27.0	41.0
66-67	37.69747340454866	41.0	37.0	41.0	27.0	41.0
68-69	37.70761238957809	41.0	37.0	41.0	27.0	41.0
70-71	37.70843386729385	41.0	37.0	41.0	27.0	41.0
72-73	37.52791788928757	41.0	37.0	41.0	27.0	41.0
74-75	37.47743914248667	41.0	37.0	41.0	27.0	41.0
76-77	36.38929859832607	39.0	34.5	41.0	24.5	41.0
78-79	36.91015980873952	41.0	37.0	41.0	27.0	41.0
80-81	37.576376912682036	41.0	37.0	41.0	27.0	41.0
82-83	37.76887887703732	41.0	37.0	41.0	27.0	41.0
84-85	37.69236608082335	41.0	37.0	41.0	27.0	41.0
86-87	37.53921938440348	41.0	37.0	41.0	27.0	41.0
88-89	37.43673275988718	41.0	37.0	41.0	27.0	41.0
90-91	37.27453502905393	41.0	37.0	41.0	27.0	41.0
92-93	37.17480732553415	41.0	37.0	41.0	27.0	41.0
94-95	37.01901244437268	41.0	37.0	41.0	27.0	41.0
96-97	36.9562117524255	41.0	37.0	41.0	27.0	41.0
98-99	36.836077695380006	41.0	37.0	41.0	27.0	41.0
100	36.71204117800434	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.361565492956105
1101	2	-0.5099024514208903
1101	3	-0.10943385358015867
1101	4	0.058942634202402644
1101	5	-1.1513640828646992
1101	6	-0.6803002439058687
1101	7	-0.20670228032224003
1101	8	-0.19574675193708657
1101	9	-0.19716885234343806
1101	10-11	-0.0932228684130294
1101	12-13	-0.05663887591506267
1101	14-15	0.07266080686270016
1101	16-17	-0.06348110181816935
1101	18-19	-0.022799415881358698
1101	20-21	0.014921071037491629
1101	22-23	0.03196535575212067
1101	24-25	-0.0834914536514475
1101	26-27	-0.048451035380828955
1101	28-29	-0.11403001111847288
1101	30-31	0.0028951124629443825
1101	32-33	-0.1773884147207596
1101	34-35	-0.03418943985009548
1101	36-37	-0.21921287235895193
1101	38-39	0.019629801632881083
1101	40-41	-0.05081353839393188
1101	42-43	0.017915603884326003
1101	44-45	-0.042431545679392
1101	46-47	-0.10159025533376109
1101	48-49	0.09687799019887677
1101	50-51	0.11095484065670291
1101	52-53	-8.441875196538717E-4
1101	54-55	-0.24684510611297839
1101	56-57	0.09646941258920805
1101	58-59	0.0042749189650308494
1101	60-61	0.0868372916049438
1101	62-63	-0.13798018978690152
1101	64-65	0.02535887950974569
1101	66-67	0.04681784055377136
1101	68-69	-0.042388893609768274
1101	70-71	-0.13830651300381902
1101	72-73	0.029957035154538403
1101	74-75	-0.015960945085929268
1101	76-77	-0.5671782824137992
1101	78-79	-0.21287393653560116
1101	80-81	-0.0194046967000574
1101	82-83	-0.022381720641170944
1101	84-85	0.09330496905561603
1101	86-87	-0.17778159355505352
1101	88-89	-0.24761175318408846
1101	90-91	-0.17680827600229065
1101	92-93	-0.0036075164660331893
1101	94-95	0.1006199667372698
1101	96-97	-0.11987290121883376
1101	98-99	-0.3720331247684854
1101	100	-0.5032862228624353
1102	1	1.8580047411565364
1102	2	0.6899527401567394
1102	3	-0.051916911596855186
1102	4	0.08269603238341716
1102	5	-0.7622065005933436
1102	6	-0.22551674905544772
1102	7	-0.28581931021418683
1102	8	-0.396728649379007
1102	9	-0.3734113898927731
1102	10-11	-0.4029879536272176
1102	12-13	-0.6028300531382911
1102	14-15	-0.2582343410014474
1102	16-17	-0.281998441196734
1102	18-19	-0.3242041478954576
1102	20-21	-0.2900843028301594
1102	22-23	-0.2307400027616211
1102	24-25	-0.16479867329962872
1102	26-27	-0.2815707687647375
1102	28-29	-0.3543562967071736
1102	30-31	-0.40361765385961945
1102	32-33	-0.5081608818528522
1102	34-35	-0.435849543473914
1102	36-37	-0.5037234138701976
1102	38-39	-0.3328857554881708
1102	40-41	-0.41659667799708444
1102	42-43	-0.4745161991762288
1102	44-45	-0.38739621663913226
1102	46-47	-0.5114338411636155
1102	48-49	-0.2870487265034569
1102	50-51	-0.3140774477077386
1102	52-53	-0.14987643975725717
1102	54-55	-0.2665402557834753
1102	56-57	-0.30480082220515925
1102	58-59	-0.24640810412905267
1102	60-61	-0.2978273910960212
1102	62-63	-0.4169753668535918
1102	64-65	-0.34533261833919937
1102	66-67	-0.28027834415072306
1102	68-69	-0.5353595887203113
1102	70-71	-0.5976148947320681
1102	72-73	-0.43498337061010517
1102	74-75	-0.5377894600018038
1102	76-77	-0.40039834304668176
1102	78-79	-0.5136584911066961
1102	80-81	-0.5231859727603094
1102	82-83	-0.5973750221226695
1102	84-85	-0.39563758324550946
1102	86-87	-0.48813185833469674
1102	88-89	-0.49825827311556736
1102	90-91	-0.4926498206656049
1102	92-93	-0.5076390461668154
1102	94-95	-0.35657499477258625
1102	96-97	-0.28034531988075884
1102	98-99	-0.4583841783174947
1102	100	-0.4465520291081617
1103	1	1.9471241406737256
1103	2	0.7626672915995965
1103	3	0.5014175506609675
1103	4	1.1329147943208078
1103	5	2.825104650686473
1103	6	0.11671382195718394
1103	7	-0.30220709315089067
1103	8	-0.1883669944807238
1103	9	-0.3233178598115316
1103	10-11	-0.3289904976454281
1103	12-13	-0.3209838152791491
1103	14-15	-0.3907644314335528
1103	16-17	-0.5000923440855942
1103	18-19	-0.29343360442333477
1103	20-21	-0.16303737238897043
1103	22-23	-0.13722055441188274
1103	24-25	-0.3049391587634176
1103	26-27	-0.44913296134613034
1103	28-29	-0.4141274663385772
1103	30-31	-0.27488718195605344
1103	32-33	-0.5460280284437005
1103	34-35	-0.4759744077732577
1103	36-37	-0.45873734821142165
1103	38-39	-0.3474252527492183
1103	40-41	-0.5658108284092052
1103	42-43	-0.3097352302177043
1103	44-45	-0.708558300003638
1103	46-47	-0.6161218970112401
1103	48-49	-0.5256839355579572
1103	50-51	-0.3186236003724616
1103	52-53	-0.38268364177042
1103	54-55	-0.33735703265155337
1103	56-57	-0.582216687794876
1103	58-59	-0.6860526504392439
1103	60-61	-0.48913230966249444
1103	62-63	-0.4806435575683139
1103	64-65	-0.5802174352237159
1103	66-67	-0.7034656655410885
1103	68-69	-0.5662936949731048
1103	70-71	-0.5683057833925531
1103	72-73	-0.7197284502714822
1103	74-75	-0.49138672537912953
1103	76-77	-0.4047395827895528
1103	78-79	-0.564438170990968
1103	80-81	-0.29781602026702814
1103	82-83	-0.3929783863176439
1103	84-85	-0.6058107681384044
1103	86-87	-0.3605845255500739
1103	88-89	-0.34364996396878666
1103	90-91	-0.7388652325852121
1103	92-93	-0.5575384274899449
1103	94-95	-0.47445781503598994
1103	96-97	-0.5033711788150796
1103	98-99	-0.5594813353968391
1103	100	-0.6694082868284923
1104	1	1.7084119204621757
1104	2	-0.6147128907228279
1104	3	0.016577914258286697
1104	4	0.2811912162759693
1104	5	-1.1725829290239353
1104	6	-0.3989346901588
1104	7	-0.2904079882644268
1104	8	-0.25768356036636675
1104	9	-0.15305197076056487
1104	10-11	-0.10998072681858417
1104	12-13	-0.14026450329620843
1104	14-15	-0.13718554857086218
1104	16-17	-0.13732964345105358
1104	18-19	0.05067299736636954
1104	20-21	0.026487206569832722
1104	22-23	0.026525383139670566
1104	24-25	-0.11532698019114207
1104	26-27	-0.19361156687551073
1104	28-29	-0.008800650381573405
1104	30-31	-0.1619507224469814
1104	32-33	-0.14375633084437567
1104	34-35	-0.12442746930773296
1104	36-37	-0.07708364035154602
1104	38-39	-0.1386461739986018
1104	40-41	-0.07481643530822879
1104	42-43	-0.2894053109560062
1104	44-45	-0.1483776274376396
1104	46-47	-0.5017670832226173
1104	48-49	-0.557712527862293
1104	50-51	-0.2583661963064756
1104	52-53	-0.2630224388404869
1104	54-55	-0.181707577759461
1104	56-57	5.561738710539998E-4
1104	58-59	-0.021421681815354532
1104	60-61	-0.08546229177594
1104	62-63	-0.16294555494425822
1104	64-65	-0.30364660000265076
1104	66-67	-0.6915119191845278
1104	68-69	-0.4490317262994665
1104	70-71	-0.28217624595517066
1104	72-73	-0.19371948342315193
1104	74-75	-0.41370983046462584
1104	76-77	-0.4396372261111523
1104	78-79	-0.35463604922096437
1104	80-81	-0.41568385353183857
1104	82-83	-0.3003528596069671
1104	84-85	-0.2524010612108327
1104	86-87	-0.2675631638147351
1104	88-89	-0.3639728124096706
1104	90-91	-0.2824447963417498
1104	92-93	-0.276850148553919
1104	94-95	-0.3178390029862683
1104	96-97	-0.2288430251635205
1104	98-99	-0.5616025816421057
1104	100	-0.45268631935950765
1105	1	1.8272171165382094
1105	2	0.26734792587330247
1105	3	-0.18252182398082084
1105	4	-0.21840024102275635
1105	5	-1.1715456709081238
1105	6	0.27394492429431594
1105	7	-0.1821255800642163
1105	8	-0.1967666633338041
1105	9	-0.03895308874867709
1105	10-11	0.06463556106947266
1105	12-13	-0.12778633709027076
1105	14-15	-0.035927139196395785
1105	16-17	0.07435712416887696
1105	18-19	-0.059052276014746496
1105	20-21	-0.06990293947806947
1105	22-23	-0.09891386142328429
1105	24-25	-0.08890209578181896
1105	26-27	-0.14075812851163505
1105	28-29	-0.11383771278830324
1105	30-31	-0.0036746747918172673
1105	32-33	-0.22021130552450785
1105	34-35	-0.02975075624170387
1105	36-37	-0.10758661268317837
1105	38-39	-0.09379919403432524
1105	40-41	-0.053416381488233355
1105	42-43	0.025754803533807547
1105	44-45	0.04785130325688414
1105	46-47	-0.23608687185681276
1105	48-49	-0.24435807425757616
1105	50-51	-0.23740599864030543
1105	52-53	-0.19396428878054195
1105	54-55	-0.3099024061470672
1105	56-57	-0.08452834798719522
1105	58-59	-0.0620667878652128
1105	60-61	-0.1754062700522354
1105	62-63	-0.1547644261921448
1105	64-65	-0.25485094474419157
1105	66-67	-0.3409597749469313
1105	68-69	-0.29585683577423083
1105	70-71	-0.360681762504818
1105	72-73	-0.21650613606399816
1105	74-75	-0.36458929813592533
1105	76-77	-0.3317917384285991
1105	78-79	-0.4440452524403753
1105	80-81	-0.4789018000543237
1105	82-83	-0.19249758647048765
1105	84-85	-0.4387662543512292
1105	86-87	-0.30509258133559314
1105	88-89	-0.3620458118959391
1105	90-91	-0.1489836812892804
1105	92-93	-0.4360998488743917
1105	94-95	-0.1791492752665249
1105	96-97	-0.125864332989444
1105	98-99	-0.4667614729501679
1105	100	-0.4904679569552215
1106	1	1.2527569255522604
1106	2	-0.5558380117217343
1106	3	-0.05787612116468921
1106	4	-0.26615281081778086
1106	5	-1.1933934903838264
1106	6	-0.5057886685528352
1106	7	-0.15019808173686755
1106	8	-0.08270425837060458
1106	9	-0.2890875087961149
1106	10-11	-0.08537173775663831
1106	12-13	-0.13143939955605788
1106	14-15	-0.00592105684130928
1106	16-17	-0.038206588500671046
1106	18-19	-0.036415575084149054
1106	20-21	-0.05358580548377745
1106	22-23	-0.0627305392409312
1106	24-25	-0.18885404517662607
1106	26-27	-0.08398968376983618
1106	28-29	-0.12531815801669666
1106	30-31	-0.016122214479189267
1106	32-33	-0.06242893218955459
1106	34-35	-0.18062661537435787
1106	36-37	-0.3485597894972443
1106	38-39	-0.02350724099010648
1106	40-41	-0.005408961015028524
1106	42-43	0.0905987911258066
1106	44-45	-0.16129655879529992
1106	46-47	-0.3174280311536961
1106	48-49	-0.1619372969325923
1106	50-51	-0.05328824424580603
1106	52-53	-0.09369788162769765
1106	54-55	-0.1517271126734272
1106	56-57	-0.09725871718615764
1106	58-59	-0.2056134091169568
1106	60-61	-0.12707466005284118
1106	62-63	-0.12135769910742056
1106	64-65	-0.37157194711630837
1106	66-67	-0.22461709506128358
1106	68-69	-0.20805743184502035
1106	70-71	-0.40591867023549355
1106	72-73	-0.31370825272357195
1106	74-75	-0.5039603826198373
1106	76-77	-0.1800369789857399
1106	78-79	-0.09650617330893851
1106	80-81	-0.15558022594083099
1106	82-83	-0.028727134551495226
1106	84-85	-0.36194002723609486
1106	86-87	-0.12483773065741133
1106	88-89	-0.1771367431044979
1106	90-91	-0.26388362654807906
1106	92-93	-0.29022368562398526
1106	94-95	-0.28896072225879976
1106	96-97	-0.08212794225894982
1106	98-99	-0.4353497583342687
1106	100	-0.5861738142631694
1107	1	1.4474998522636078
1107	2	-0.5212985442177178
1107	3	-0.18168513203780812
1107	4	-0.20544070359212263
1107	5	-1.2465441396271153
1107	6	-0.3837880447983082
1107	7	-0.1191994858108103
1107	8	-0.3130006920728903
1107	9	-0.18116695357943513
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2228	1	1.3889367130036696
2228	2	1.671135275691519
2228	3	-0.35908263104989757
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2228	90-91	0.2637673841285846
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2228	98-99	0.7847305935993205
2228	100	0.7414464243801007
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	23.0
13	36.0
14	118.0
15	399.0
16	1528.0
17	3968.0
18	6304.0
19	8030.0
20	9463.0
21	10944.0
22	12639.0
23	14213.0
24	16223.0
25	18458.0
26	20787.0
27	23461.0
28	26341.0
29	29868.0
30	34290.0
31	39257.0
32	46456.0
33	55542.0
34	67698.0
35	87792.0
36	122304.0
37	202686.0
38	494630.0
39	912204.0
40	985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.753840091389996	18.96959372052016	19.182893432737202	34.09367275535264
2	19.27881851468981	53.3281888442239	10.077639429552928	17.315353211533363
3	40.70342669149629	14.988438870278435	24.508359704885674	19.799774733339596
4	17.678050441908248	16.401804074476527	22.261031382478173	43.65911410113705
5	16.883264134203518	35.1592903526663	26.811541453080256	21.145904060049933
6	17.05761858816128	27.631210329619037	31.2583300355106	24.05284104670908
7	20.139571799225905	26.6342310911227	36.86864341911202	16.357553690539373
8	20.208528279877722	27.593489414099327	35.154878549681534	17.043103756341416
9	20.42011835102687	28.04000799418701	28.18003862092333	23.359835033862794
10-11	19.148173491505293	30.425094864793678	28.882728541321168	21.544003102379857
12-13	24.06748823261849	26.58812774993195	27.408811341157225	21.935572676292338
14-15	21.751865200006883	27.385362608293217	28.603968769729033	22.258803421970867
16-17	20.826268933803984	30.03414294329907	28.149133941015076	20.990454181881873
18-19	21.360979455557043	29.7218093509929	29.81357485307593	19.10363634037413
20-21	22.65061564504751	29.110046690110984	29.964171747960755	18.275165916880752
22-23	22.12625080129372	30.29973348298169	28.683050338230874	18.890965377493714
24-25	22.23308261056971	28.415165660996177	26.363699332097145	22.98805239633697
26-27	20.0255928691146	26.349669798605607	30.18171775313933	23.44301957914047
28-29	19.185816173557487	28.25960740091713	30.98527422552697	21.56930219999841
30-31	19.87176206267881	30.35915358725598	28.29474517431076	21.47433917575445
32-33	20.30814679127363	30.395712256915168	29.01545763411771	20.280683317693494
34-35	21.363428006213585	29.43268184238657	29.908847738531847	19.295042412867993
36-37	21.734438578217073	28.124074900061636	28.879618220216912	21.26186830150438
38-39	21.557739272649705	29.242007222634363	28.96075199045982	20.239501514256112
40-41	20.69196456638112	30.041412165201297	29.874135518569737	19.392487749847845
42-43	20.29113065982307	30.352033434429863	29.392223000905965	19.964612904841097
44-45	20.605458194416688	29.96540705279649	28.96326600480798	20.465868747978845
46-47	19.738960403531117	28.044495014760052	29.149303954705548	23.067240627003283
48-49	20.635529183752297	28.010124207722733	30.546757070412173	20.807589538112804
50-51	21.74705633475349	30.876775254373527	28.242906813456177	19.1332615974168
52-53	20.326875458420883	30.5072878579819	28.36241307237121	20.803423611226005
54-55	22.385331059900025	28.30512722754102	28.454188906775112	20.85535280578384
56-57	21.731685126337926	28.877082504185086	29.41946679774195	19.97176557173504
58-59	21.10157929478875	27.99740372973374	29.19784779608247	21.703169179395037
60-61	19.49080214863961	30.316964268973045	29.91440868402993	20.277824898357416
62-63	20.38782174248864	31.496937477234876	27.243910418975354	20.871330361301133
64-65	19.87189880376089	30.181009778547196	29.066146426514422	20.88094499117749
66-67	20.095586123467836	30.38838866396047	29.553829952348114	19.96219526022358
68-69	20.702892863335137	30.00639270252492	29.418056715492092	19.872657718647854
70-71	19.525492941776996	31.21317523196157	29.233996294967852	20.02733553129358
72-73	20.06320348955969	30.763325740620395	28.652278012412168	20.521192757407746
74-75	19.835841222740022	29.153480890495963	30.896297482581097	20.114380404182917
76-77	19.372822499489335	30.36835907840965	28.83916198684665	21.419656435254364
78-79	19.397815363398006	29.571830108525944	28.608579103848108	22.421775424227945
80-81	20.55071286006978	29.737400315179624	28.568844511974532	21.14304231277607
82-83	19.544728402790554	28.71095499210949	30.418565396376234	21.325751208723723
84-85	20.443787674040113	29.327261810065703	28.260179022141518	21.968771493752666
86-87	21.19341917819581	28.801683720491106	29.4927485400241	20.51214856128899
88-89	21.743659246455227	28.376849152073525	29.136693980138944	20.742797621332304
90-91	21.486936430771973	28.917890611109716	28.85936804451686	20.735804913601456
92-93	21.10502870539612	28.5301813648089	29.896869693428226	20.46792023636676
94-95	21.387163506271598	29.153767657689972	29.172208994166272	20.286859841872158
96-97	20.61284796441616	28.86216953941218	29.649919021356215	20.875063474815445
98-99	22.268928509820892	29.234613947385718	28.412209752996386	20.084247789797
100	21.88995463342991	31.051460593555152	27.41816436348492	19.640420409530023
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	5.0
2	13.0
3	18.5
4	21.5
5	25.0
6	33.0
7	37.5
8	43.5
9	54.0
10	61.0
11	79.5
12	89.0
13	93.5
14	118.5
15	163.0
16	214.5
17	257.0
18	407.5
19	686.0
20	1069.0
21	1622.0
22	2460.5
23	3883.5
24	6043.0
25	8953.0
26	12734.5
27	17057.5
28	21855.5
29	27444.5
30	34100.5
31	41555.0
32	50017.5
33	59535.5
34	69931.0
35	80901.5
36	91567.0
37	99715.0
38	104606.5
39	108069.5
40	110303.0
41	110374.0
42	108484.0
43	106811.0
44	106164.0
45	103060.5
46	94734.0
47	85505.5
48	80141.5
49	76783.0
50	72923.0
51	67807.0
52	59738.0
53	50373.0
54	41932.5
55	34303.5
56	26717.5
57	19307.5
58	13463.5
59	9527.5
60	7440.5
61	6276.5
62	5165.5
63	3958.0
64	3064.5
65	2697.5
66	2530.0
67	2114.5
68	1560.5
69	1546.0
70	1796.5
71	1651.5
72	1056.0
73	504.5
74	250.0
75	163.5
76	119.0
77	95.5
78	77.0
79	50.5
80	40.0
81	32.5
82	22.5
83	18.0
84	18.5
85	17.0
86	14.0
87	14.0
88	10.0
89	8.0
90	10.5
91	14.0
92	10.5
93	8.0
94	12.5
95	11.0
96	11.5
97	17.5
98	34.5
99	85.0
100	92.0
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.329465946836892
2	0.006352996298056116
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.9706226862850724E-4
30-31	0.0103677370141888
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.92655671571268E-4
40-41	0.0018750162685235063
42-43	6.617704477141787E-5
44-45	0.0
46-47	0.002470609671466267
48-49	2.2059014923805955E-5
50-51	0.0
52-53	0.0015220720297426111
54-55	0.020779592058225212
56-57	0.011691277909617156
58-59	0.0140736515213882
60-61	1.544131044666417E-4
62-63	0.013080995849816934
64-65	6.617704477141787E-5
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.3235408954283574E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2266647.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.30565251083009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.70888564439882	65.296692119384
2	8.251864450636754	12.428240737722462
3	2.303562587351432	5.204138512201068
4	0.968337499466676	2.9168514899217444
5	0.5294297049267238	1.9934524694061584
6	0.32382536548546326	1.4631528268464504
7	0.20258660403427112	1.0679141484727765
8	0.13898079930476043	0.8372831822497364
9	0.09608058309273838	0.6511869903077725
>10	0.4474736505692535	5.735299809922953
>50	0.019706955346550474	1.0167972381904484
>100	0.00884854328098115	1.161123252072527
>500	3.579532334087767E-4	0.1797958650739273
>1k	5.96588722345558E-5	0.04807135822801311
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.411802984761191E-5	0.0
3	0.0	0.0	0.0	4.411802984761191E-5	0.0
4	0.0	0.0	0.0	4.411802984761191E-5	0.0
5	0.0	0.0	0.0	4.411802984761191E-5	0.0
6	0.0	0.0	0.0	4.411802984761191E-5	0.0
7	0.0	0.0	0.0	4.411802984761191E-5	0.0
8	0.0	0.0	0.0	4.411802984761191E-5	0.0
9	0.0	0.0	0.0	4.411802984761191E-5	0.0
10-11	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
12-13	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
14-15	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
16-17	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
18-19	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
20-21	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
22-23	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
24-25	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
26-27	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
28-29	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
30-31	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
32-33	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
34-35	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
36-37	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
38-39	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
40-41	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
42-43	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
44-45	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
46-47	4.411802984761191E-5	0.0	0.0	4.411802984761191E-5	0.0
48-49	8.823605969522382E-5	0.0	0.0	4.411802984761191E-5	0.0
50-51	8.823605969522382E-5	0.0	0.0	4.411802984761191E-5	0.0
52-53	8.823605969522382E-5	0.0	0.0	4.411802984761191E-5	0.0
54-55	1.1029507461902979E-4	0.0	0.0	4.411802984761191E-5	0.0
56-57	1.7647211939044767E-4	0.0	0.0	4.411802984761191E-5	0.0
58-59	2.647081790856715E-4	0.0	0.0	4.411802984761191E-5	0.0
60-61	3.088262089332834E-4	0.0	0.0	4.411802984761191E-5	0.0
62-63	3.3088522385708933E-4	0.0	0.0	4.411802984761191E-5	0.0
64-65	3.529442387808953E-4	0.0	2.2059014923805955E-5	4.411802984761191E-5	0.0
66-67	4.4118029847611915E-4	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
68-69	4.4118029847611915E-4	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
70-71	4.632393133999251E-4	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
72-73	5.955934029427608E-4	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
74-75	6.617704477141787E-4	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
76-77	8.161835521808204E-4	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
78-79	9.92655671571268E-4	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
80-81	0.0011470687760379097	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
82-83	0.0012353048357331335	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
84-85	0.0013676589252759692	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
86-87	0.0013676589252759692	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
88	0.0013676589252759692	0.0	4.411802984761191E-5	4.411802984761191E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	9445	0.0	80.0599	8
CACCGGC	9120	0.0	78.5897	9
CTTCACC	10300	0.0	76.19395	6
GTGTGGC	4385	0.0	72.36	7
CCTTCAC	11335	0.0	72.30097	5
TGTGGCA	4730	0.0	67.28062	8
CGTAGTG	1650	0.0	67.13508	4
GAGCAGC	40	3.9482893E-6	66.22815	1
CGGGCCC	615	0.0	65.636406	7
TTCACCG	12015	0.0	64.77124	7
CAGCGTA	880	0.0	64.421936	1
TAGCTGC	25	0.0032583226	63.579025	1
ACGGGCC	635	0.0	62.090767	6
AACGGGC	650	0.0	61.380016	5
ACCTTCA	4480	0.0	60.872276	4
TGTGTGG	4990	0.0	59.730286	6
GTGGCAC	4835	0.0	59.60646	9
GCCTTCA	3345	0.0	59.215824	4
GCGTAGT	1270	0.0	57.655712	3
ATGTGTG	4875	0.0	57.288013	5
>>END_MODULE