FastQCFastQC Report
Mon 6 Mar 2017
1NF_12A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_12A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2179923
Sequences flagged as poor quality0
Sequence length96
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA127920.5868097175909425No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA58920.2702847761136517No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA54780.2512932796250143No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT52270.2397791114640288No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG51710.23721021338827106No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG45580.20908995409470885No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG44480.2040439043030419No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT40770.18702495455114698No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG39980.18340097333713162No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC35840.1644094768484942No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC34470.15812485119887262No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA34150.1566569094412968No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT33360.15303292822728143No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG32140.14743640027652352No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA31650.1451886144602355No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA30570.14023431102841705No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA27460.12596775207197686No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC26800.12294012219697667No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC26460.12138043407955235No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG26330.12078408274053716No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA24770.11362786667235493No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA23060.10578355290530904No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA22520.10330640118939982No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC22220.10193020579167245No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCAA850.090.000013
AGCAGCG850.090.000012
AGCAGCC700.090.000012
GCTGCTA256.377286E-790.03
GCTGCAG256.377286E-790.03
GCAGCCG202.2811397E-590.03
GCAGCAC401.4551915E-1190.03
TGCAGCA256.377286E-790.02
CTGCTGC1200.089.999991
GCTGCTC158.2052214E-489.999993
GCAGCAT301.7940692E-889.999993
TGCAGCC158.2052214E-489.999992
AGCAGCA1450.086.896562
AGCTGCT1050.085.714292
CAGCAGC3850.085.324671
CAGCGTA46950.085.1118241
CTGCAGC850.084.705891
CAGCCCT142700.084.3552861
AGCTGCA700.083.5714342
CAGCTGC4100.083.414641
Produced by FastQC (version 0.11.5)