FastQCFastQC Report
Tue 7 Mar 2017
1NF_12A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_12A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2179923
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG90550.078.117158
CACCGGC89200.076.036949
AGCTGCG351.7951752E-675.6263661
GTGTGGC40000.074.278887
CTTCACC101900.073.0551156
CCTTCAC112650.070.666945
CGTAGTG16650.069.913184
TGTGGCA41800.068.834448
GCCTTCA29950.064.882134
TTCACCG113300.064.337357
TGTGTGG43900.063.403296
GGCGCGC2900.063.1230936
CAGCGTA8200.061.976731
CGGGGCA10800.061.71436394
CGGGCCC5400.060.8451467
ACCTTCA43700.060.4712034
ATGTGTG42250.059.3247835
GCGTAGT12900.059.308853
GTGGCAC46350.057.823989
AGGCGCG3300.056.8941575
Produced by FastQC (version 0.11.5)