FastQCFastQC Report
Tue 7 Mar 2017
1NF_13A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_13A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3890118
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA179220.4607058192065125No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG161480.4151030894178532No Hit
CTGCATAACCCTACCCCTAACCCTAATTCCAATGCTAACCCTAACCCTAA137100.35243146865981956No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC91840.23608538352821173No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG85100.21875943094785297No Hit
CTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAA78640.202153250878251No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG77070.19811738358579356No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT74590.19174225563337668No Hit
CAGCTAGGGTTAGGGCTACAATTAGGGTTAGGGTTGGAATTAGGGTTAGG71260.1831821039875911No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA69720.17922335517842905No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG66450.17081744049923422No Hit
CAGCAGGCGCGCAAATTACCCACTCCTGGCACGGGGAGGTAGTGACGAAA64780.1665245115957922No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG63750.16387677700265135No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA63040.162051639564661No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT60700.15603639786762252No Hit
CTGCGCCGAGCGACAGAGCAGGCACGGACGAAACAGGACGTCCGAGCTCA60610.15580504241773643No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC56080.14416015144013627No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC55940.14380026518475789No Hit
CAGCAGAAGAGCGGCCGGGAGAGTTGAGGGTGGACGTCTCGAGCTCGAGG55470.1425920756131305No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA53320.13706525097696265No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA50850.13071582918564423No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA48050.1235181040780768No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT46490.11950794294671781No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA40400.10385289083775864No Hit
CAGCGGTTCTGACGTGCAAATCGATCGTCAAACTTGAGCATAGGGGCGAA40220.10339017993798646No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA40020.10287605671601735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCAC202.4114666E-588.9998253
GCAGCTA450.088.9998253
GCTGCTA700.088.999823
GCTGCCC2650.088.999823
TGCTGCC750.088.999822
AGCTGCG750.088.999822
GCAGCTC256.8169174E-788.999823
GCAGCAC500.088.999823
CAGCTGC6350.087.598251
AGCTGCC3150.086.174432
AGCAGCC1350.085.703522
AGCTGCA1250.085.439822
AGCAGCA2100.084.7617342
AGCAGCG900.084.055392
CTGCTGC2650.083.962091
CAGCCCT194650.083.1244051
GCAGCCA750.083.06653
GCTGCCA700.082.642693
CAGCGTA79600.082.179481
GCAGCCG600.081.5831763
Produced by FastQC (version 0.11.5)