FastQCFastQC Report
Tue 7 Mar 2017
1NF_13A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_13A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences3890118
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG138300.079.82538
CTTCACC155050.073.441996
CACCGGC150400.071.998799
GAGCTGC601.2551027E-1070.627051
CGCTGCA900.070.627051
CCTTCAC171950.069.035445
CGGGCCC12100.068.6615147
CGGGGCA14000.068.3955194
TTCACCG167250.067.355117
GGCGCGC11350.066.995346
CGTAGTG29950.065.823044
GGGCCCG12850.064.654038
CAGCTGC1400.064.321071
AACGGGC12750.064.0566945
AGGCGCG12100.062.842745
GCGCGCA12150.062.1978077
GTGTGGC56650.061.976517
GGCCCGA13800.060.543349
ATGCAGC1100.059.7432332
ACGGGCC14000.059.343166
Produced by FastQC (version 0.11.5)