##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	1NF_13A_2.fq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	3890118
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	13.787433954445598	12.0	12.0	12.0	2.0	27.0
2	14.608379745807197	12.0	12.0	12.0	12.0	22.0
3	13.69631743818568	12.0	12.0	12.0	12.0	22.0
4	13.822187656004266	12.0	12.0	12.0	12.0	22.0
5	22.449787127279944	22.0	22.0	22.0	22.0	27.0
6	26.84043723095289	27.0	22.0	32.0	22.0	32.0
7	34.99793039696996	37.0	37.0	37.0	27.0	41.0
8	37.64247382727208	41.0	37.0	41.0	32.0	41.0
9	38.580661563479566	41.0	41.0	41.0	32.0	41.0
10-11	38.91616578211766	41.0	41.0	41.0	37.0	41.0
12-13	39.10210320098259	41.0	41.0	41.0	37.0	41.0
14-15	39.06037117640133	41.0	41.0	41.0	37.0	41.0
16-17	38.965451947730116	41.0	41.0	41.0	37.0	41.0
18-19	38.90725345606482	41.0	41.0	41.0	37.0	41.0
20-21	38.90816898099235	41.0	41.0	41.0	37.0	41.0
22-23	38.900345953516066	41.0	41.0	41.0	37.0	41.0
24-25	38.924673364664	41.0	41.0	41.0	37.0	41.0
26-27	38.894194083572785	41.0	41.0	41.0	37.0	41.0
28-29	38.839000256547486	41.0	41.0	41.0	37.0	41.0
30-31	38.72194622373923	41.0	41.0	41.0	32.0	41.0
32-33	38.76567985855442	41.0	41.0	41.0	32.0	41.0
34-35	38.741301677738306	41.0	41.0	41.0	32.0	41.0
36-37	38.665336629891435	41.0	41.0	41.0	32.0	41.0
38-39	38.56682188046738	41.0	41.0	41.0	32.0	41.0
40-41	38.608139778793344	41.0	41.0	41.0	32.0	41.0
42-43	38.351238188661625	41.0	41.0	41.0	32.0	41.0
44-45	38.45544980897752	41.0	41.0	41.0	32.0	41.0
46-47	38.47405078714836	41.0	41.0	41.0	32.0	41.0
48-49	38.40883965473541	41.0	41.0	41.0	32.0	41.0
50-51	38.315862655065985	41.0	41.0	41.0	32.0	41.0
52-53	38.2724121736153	41.0	41.0	41.0	32.0	41.0
54-55	38.15550595123335	41.0	39.0	41.0	32.0	41.0
56-57	37.94612554169308	41.0	37.0	41.0	29.5	41.0
58-59	37.94685456842183	41.0	37.0	41.0	29.5	41.0
60-61	37.947561359321234	41.0	37.0	41.0	32.0	41.0
62-63	37.93891419232013	41.0	37.0	41.0	32.0	41.0
64-65	37.92563528921231	41.0	37.0	41.0	32.0	41.0
66-67	37.791206847710015	41.0	37.0	41.0	27.0	41.0
68-69	37.81343933525923	41.0	37.0	41.0	27.0	41.0
70-71	37.785999679187114	41.0	37.0	41.0	27.0	41.0
72-73	37.62770538065941	41.0	37.0	41.0	27.0	41.0
74-75	37.55717628616921	41.0	37.0	41.0	27.0	41.0
76-77	36.51907062973411	39.0	34.5	41.0	24.5	41.0
78-79	37.030772588389354	41.0	37.0	41.0	27.0	41.0
80-81	37.67783663631798	41.0	37.0	41.0	27.0	41.0
82-83	37.85503203244734	41.0	37.0	41.0	32.0	41.0
84-85	37.777080155409166	41.0	37.0	41.0	27.0	41.0
86-87	37.642721634665065	41.0	37.0	41.0	27.0	41.0
88-89	37.574065234010895	41.0	37.0	41.0	27.0	41.0
90-91	37.424525297176075	41.0	37.0	41.0	27.0	41.0
92-93	37.300070717649184	41.0	37.0	41.0	27.0	41.0
94-95	37.129285152789706	41.0	37.0	41.0	27.0	41.0
96-97	37.07088795249913	41.0	37.0	41.0	27.0	41.0
98-99	36.95988103702767	41.0	37.0	41.0	27.0	41.0
100	36.82796948575853	41.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.384660385626768
1101	2	-0.5076123337128919
1101	3	-0.07479944481844569
1101	4	0.08486285626461587
1101	5	-1.0260214972389647
1101	6	-0.8579615755233974
1101	7	-0.552532641738388
1101	8	-0.18911862849301997
1101	9	-0.32046857243265947
1101	10-11	-0.23378470310301225
1101	12-13	-0.1544064175570199
1101	14-15	-0.07075876271202475
1101	16-17	-0.12055217133357843
1101	18-19	-0.036019203219225915
1101	20-21	-0.11506962053404379
1101	22-23	-0.06266267621553112
1101	24-25	-0.13587496852125014
1101	26-27	0.028991712139884385
1101	28-29	-0.11249516517720082
1101	30-31	-0.009457003770826589
1101	32-33	-0.1868629772412902
1101	34-35	-0.012005913295134008
1101	36-37	0.14403128576014979
1101	38-39	0.17215953054626
1101	40-41	0.12783262686762242
1101	42-43	0.20708227721081585
1101	44-45	0.01985704298380142
1101	46-47	0.08737510928879288
1101	48-49	-0.014380569745107152
1101	50-51	-0.05440994763218043
1101	52-53	-0.013307847426247577
1101	54-55	-0.052339382086287856
1101	56-57	0.06443369312633251
1101	58-59	0.01497103318996551
1101	60-61	-0.039991445209508925
1101	62-63	-0.12239039110326644
1101	64-65	-0.0651613556791304
1101	66-67	-0.06288387526016237
1101	68-69	-0.14080233457809044
1101	70-71	-0.19593036575924572
1101	72-73	-0.06333609972092802
1101	74-75	-0.0383513530186832
1101	76-77	-0.4096957618462014
1101	78-79	-0.11773362527924291
1101	80-81	-0.1651373401632199
1101	82-83	-0.2407090205709821
1101	84-85	-0.1365376965045897
1101	86-87	-0.23159024832899888
1101	88-89	-0.39563137976607266
1101	90-91	-0.31304418394876876
1101	92-93	-0.21233523293737733
1101	94-95	-0.15530245741695126
1101	96-97	-0.25745549762120845
1101	98-99	-0.3695796120177306
1101	100	-0.6478322030922641
1102	1	1.6690236868580826
1102	2	0.6595226538103756
1102	3	-0.020972434375330806
1102	4	0.06528924556109139
1102	5	-0.7484089883626268
1102	6	0.050621751930087555
1102	7	-0.007219540223601939
1102	8	-0.23937692004311373
1102	9	-0.15646806092773602
1102	10-11	-0.2936973148669182
1102	12-13	-0.2518127842514062
1102	14-15	-0.21456978778834213
1102	16-17	-0.30709526788583474
1102	18-19	-0.18483688439579993
1102	20-21	-0.06758023484776032
1102	22-23	-0.21140070709252967
1102	24-25	-0.16202100686367515
1102	26-27	-0.10275290008927129
1102	28-29	-0.3396923025596763
1102	30-31	-0.33996259085596847
1102	32-33	-0.25194460434313726
1102	34-35	-0.21600369632688654
1102	36-37	-0.348564630211591
1102	38-39	-0.33430559334870935
1102	40-41	-0.2542987564900656
1102	42-43	-0.2147113939045937
1102	44-45	-0.283696952545057
1102	46-47	-0.4439101959893179
1102	48-49	-0.2646060710140645
1102	50-51	-0.14538613432947045
1102	52-53	-0.18481078028351305
1102	54-55	-0.1832114862469183
1102	56-57	-0.20458127610208265
1102	58-59	-0.24677334272793416
1102	60-61	-0.18228655874533217
1102	62-63	-0.2993110946001849
1102	64-65	-0.18416246466605202
1102	66-67	-0.24392045875588764
1102	68-69	-0.4556315611514705
1102	70-71	-0.4562805052918222
1102	72-73	-0.31232662330453564
1102	74-75	-0.5521364693315789
1102	76-77	-0.29932248559851615
1102	78-79	-0.2247668682388877
1102	80-81	-0.2511579458339881
1102	82-83	-0.31579017505032425
1102	84-85	-0.306244999041823
1102	86-87	-0.23505208246967157
1102	88-89	-0.2545588417647906
1102	90-91	-0.3401713329775333
1102	92-93	-0.30999869286593906
1102	94-95	-0.1506808545797398
1102	96-97	-0.23302510410599098
1102	98-99	-0.2548120777647469
1102	100	-0.5574771714139857
1103	1	1.4962076392634014
1103	2	0.6579590019939214
1103	3	0.299372384239323
1103	4	1.1618471301648015
1103	5	2.8030345493237228
1103	6	0.02052470665096351
1103	7	-0.5637777717273309
1103	8	-0.3701540628011415
1103	9	-0.36602914568408096
1103	10-11	-0.4678521363434953
1103	12-13	-0.48902023913272075
1103	14-15	-0.35996130781747837
1103	16-17	-0.40207475742246146
1103	18-19	-0.34829339614270083
1103	20-21	-0.302363465701049
1103	22-23	-0.16038073432417832
1103	24-25	-0.34862572296937344
1103	26-27	-0.30838022540343246
1103	28-29	-0.32736642468582744
1103	30-31	-0.14156717687415465
1103	32-33	-0.33164843845554515
1103	34-35	-0.3100687574246521
1103	36-37	-0.28416897339977254
1103	38-39	-0.25369236084765134
1103	40-41	-0.37270657214740055
1103	42-43	-0.23887254630643895
1103	44-45	-0.5808587033494348
1103	46-47	-0.58063719435021
1103	48-49	-0.42975146548274523
1103	50-51	-0.35343542882156953
1103	52-53	-0.27779897701051226
1103	54-55	-0.19058449916027342
1103	56-57	-0.20325698529580194
1103	58-59	-0.6208991113282849
1103	60-61	-0.3546211091195559
1103	62-63	-0.33126136648307636
1103	64-65	-0.3827304614051883
1103	66-67	-0.520901387927573
1103	68-69	-0.4198905393574748
1103	70-71	-0.5012216623768211
1103	72-73	-0.3693357381024285
1103	74-75	-0.2760881808495341
1103	76-77	-0.28812724159772074
1103	78-79	-0.5975161375807261
1103	80-81	-0.33286512753154085
1103	82-83	-0.5293565673768938
1103	84-85	-0.3478034473580536
1103	86-87	-0.41138283428873024
1103	88-89	-0.40745363566281867
1103	90-91	-0.6047454698469394
1103	92-93	-0.3248360401215322
1103	94-95	-0.4265301091783371
1103	96-97	-0.40506192754255466
1103	98-99	-0.47674387722184974
1103	100	-0.5971666959007962
1104	1	1.6552678481836303
1104	2	-0.692250968085343
1104	3	-0.10451818635436716
1104	4	0.05109403748050845
1104	5	-1.0148277802943078
1104	6	-0.5579743572324318
1104	7	-0.2122252179662354
1104	8	-0.09116314400706926
1104	9	0.025450968399660212
1104	10-11	-0.13808782775747375
1104	12-13	0.01402852641818697
1104	14-15	-0.18889903419147203
1104	16-17	-0.03584494517070169
1104	18-19	-0.11181258215868439
1104	20-21	0.040941113396428364
1104	22-23	0.0538953938275597
1104	24-25	-0.02570153966549782
1104	26-27	-0.10958475375814203
1104	28-29	-0.1397306877783322
1104	30-31	-0.10228247494418241
1104	32-33	-0.018112717295814207
1104	34-35	-0.18558528381964123
1104	36-37	-0.20943686022589958
1104	38-39	-0.17422279776039318
1104	40-41	-0.1042751361270291
1104	42-43	-0.1482490226585611
1104	44-45	-0.022883952251461892
1104	46-47	-0.24662396623521943
1104	48-49	-0.38369697824717974
1104	50-51	-0.15801723841369864
1104	52-53	-0.08859765069951919
1104	54-55	-0.17775436495439578
1104	56-57	-0.19022309262550863
1104	58-59	-0.28240092232022107
1104	60-61	-0.09131486775894615
1104	62-63	-0.193877593670706
1104	64-65	-0.39646967856673854
1104	66-67	-0.42899800915904507
1104	68-69	-0.4580686989697327
1104	70-71	-0.3484436707406289
1104	72-73	-0.1712657130809916
1104	74-75	-0.31114924714758274
1104	76-77	-0.5381275915478483
1104	78-79	-0.26025957164137736
1104	80-81	-0.30000909701589507
1104	82-83	-0.42384047841957795
1104	84-85	-0.3360076782551573
1104	86-87	-0.31433186405198654
1104	88-89	-0.3279122453109409
1104	90-91	-0.22225216264320835
1104	92-93	-0.28778856938611597
1104	94-95	-0.31500450157741966
1104	96-97	-0.3044377039944166
1104	98-99	-0.33998826489660416
1104	100	-0.3284287910522323
1105	1	1.7584854087611337
1105	2	0.23148509478247448
1105	3	-0.07657242807570697
1105	4	-0.32523528940638613
1105	5	-1.0967844725426943
1105	6	0.6005255583127678
1105	7	0.3040519810778335
1105	8	0.15874537723122017
1105	9	0.11494813623726685
1105	10-11	-0.07266253513414256
1105	12-13	-0.07111554008871934
1105	14-15	0.058272984345101975
1105	16-17	0.0481221893699697
1105	18-19	0.057821109786999614
1105	20-21	0.09189846288710157
1105	22-23	0.07265075587106651
1105	24-25	0.03212842355903689
1105	26-27	0.06978678547654482
1105	28-29	0.11308516898632348
1105	30-31	0.03535108488409833
1105	32-33	-0.0536998788285743
1105	34-35	-0.06567673749576386
1105	36-37	-0.03981378784396128
1105	38-39	0.11902529197241307
1105	40-41	0.15032342763968387
1105	42-43	0.2159829905598727
1105	44-45	0.10452635424420009
1105	46-47	-0.04614851080644655
1105	48-49	-0.09078006002784633
1105	50-51	-0.0073820296263846785
1105	52-53	-0.08760288187988863
1105	54-55	-0.03774825243384328
1105	56-57	0.09436900742249321
1105	58-59	0.02757151698521909
1105	60-61	0.051228317073302776
1105	62-63	-0.005545651193614276
1105	64-65	-0.18970493284258083
1105	66-67	-0.08604095400588108
1105	68-69	-0.020506716673466485
1105	70-71	-0.15565924621228078
1105	72-73	0.010204151607368317
1105	74-75	-0.026750347902996907
1105	76-77	-0.15481853548491387
1105	78-79	-0.09386463759137342
1105	80-81	-0.11859638564894937
1105	82-83	0.023083443694702055
1105	84-85	-0.06709933596069106
1105	86-87	-0.1012987326854784
1105	88-89	-0.07635861149691436
1105	90-91	0.05329102386141216
1105	92-93	-0.036244050000810546
1105	94-95	0.0547290728004981
1105	96-97	-0.10340012244535757
1105	98-99	-0.10854833567140076
1105	100	-0.11461767211813623
1106	1	1.5286500119273025
1106	2	-0.7191012554580745
1106	3	-0.005186483314027157
1106	4	-0.23284987193073725
1106	5	-1.174072750851618
1106	6	-0.5660980534739117
1106	7	-0.060294899555238146
1106	8	-0.23156277853479423
1106	9	-0.3812051282355071
1106	10-11	-0.3072512724680365
1106	12-13	-0.11997349577216454
1106	14-15	-0.12435253221126885
1106	16-17	-0.09970551481030299
1106	18-19	-0.15479512817024244
1106	20-21	-0.12220480342503492
1106	22-23	-0.2096254847839134
1106	24-25	-0.2927359740064617
1106	26-27	-0.2194874400465494
1106	28-29	-0.34947180038611947
1106	30-31	-0.13066267360255068
1106	32-33	-0.12163945757200167
1106	34-35	-0.25324157528377356
1106	36-37	-0.37943177115882776
1106	38-39	-0.10528668150795539
1106	40-41	-0.18455920012498694
1106	42-43	-0.08735648763133952
1106	44-45	-0.1322156720873977
1106	46-47	-0.331739704720988
1106	48-49	-0.2612132111954395
1106	50-51	-0.41038449438201496
1106	52-53	-0.3351393524969737
1106	54-55	-0.4083093419948085
1106	56-57	-0.2966111314703781
1106	58-59	-0.2996965282551116
1106	60-61	-0.23395780672037603
1106	62-63	-0.3070424895211801
1106	64-65	-0.485558584400529
1106	66-67	-0.3743286641538859
1106	68-69	-0.3979797494673889
1106	70-71	-0.569347223682044
1106	72-73	-0.44395405055164616
1106	74-75	-0.40568874536220534
1106	76-77	-0.4997279771748424
1106	78-79	-0.47025060279050024
1106	80-81	-0.5205596064264668
1106	82-83	-0.5524181933444794
1106	84-85	-0.6394311255858298
1106	86-87	-0.47780160005873995
1106	88-89	-0.6499883831289281
1106	90-91	-0.5878058128311565
1106	92-93	-0.5199842247000745
1106	94-95	-0.5616047360440106
1106	96-97	-0.38137591633783785
1106	98-99	-0.6494843296873185
1106	100	-0.6434430505173978
1107	1	1.3732248188317229
1107	2	-0.5162712120245718
1107	3	-0.11149370356157995
1107	4	-0.19811056032552976
1107	5	-1.2202937698703167
1107	6	-0.15962580422769435
1107	7	-0.2927217246319884
1107	8	-0.3594437745676089
1107	9	-0.2901955052889065
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2224	40-41	0.2584830930586861
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2224	46-47	0.26151346291392485
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2224	56-57	0.42594676658698205
2224	58-59	0.41640345268650947
2224	60-61	0.4321516212652199
2224	62-63	0.47456124066467
2224	64-65	0.31428977000656033
2224	66-67	0.31225587621045037
2224	68-69	0.3608488827978036
2224	70-71	0.3784461111409101
2224	72-73	0.4605346918578661
2224	74-75	0.2621178646112412
2224	76-77	0.4577010139137201
2224	78-79	0.33138422870709405
2224	80-81	0.3588218504227214
2224	82-83	0.3237521721821892
2224	84-85	0.1487751847109564
2224	86-87	0.4008722321223388
2224	88-89	0.31829057904346314
2224	90-91	0.42958545580005847
2224	92-93	0.3431270401194624
2224	94-95	0.5100982456073737
2224	96-97	0.3113876479743496
2224	98-99	0.26295907019702014
2224	100	0.36783244828652784
2225	1	1.3418212426506724
2225	2	1.4688946591261107
2225	3	0.8142977573736516
2225	4	0.952639897425712
2225	5	3.2205657110251664
2225	6	-0.04348214934761074
2225	7	0.22337011589539202
2225	8	0.3801075342731792
2225	9	0.3025447557015397
2225	10-11	0.15400585624048801
2225	12-13	0.2808114155557391
2225	14-15	0.2703484000389196
2225	16-17	0.29625451718234075
2225	18-19	0.13271022701496094
2225	20-21	0.13373786392144638
2225	22-23	0.20413404255888423
2225	24-25	0.17629362571075546
2225	26-27	0.08470760435058367
2225	28-29	0.12138931339466552
2225	30-31	0.11078303907282816
2225	32-33	0.07250665305676307
2225	34-35	0.153175199029576
2225	36-37	0.13642210192286797
2225	38-39	0.08980479238561401
2225	40-41	0.14740746822493378
2225	42-43	0.3080146776080994
2225	44-45	0.2572643821893337
2225	46-47	0.30790251189401374
2225	48-49	0.1108790657722949
2225	50-51	0.19559734930032846
2225	52-53	0.17966506718013164
2225	54-55	0.19732577889799074
2225	56-57	0.3178154524020087
2225	58-59	0.27645632298394673
2225	60-61	0.29661195240624494
2225	62-63	0.14356201518731382
2225	64-65	0.17982093737125382
2225	66-67	0.3245638102430277
2225	68-69	0.3035211684480075
2225	70-71	0.3464730929539712
2225	72-73	0.19405212644068826
2225	74-75	0.20507521762144876
2225	76-77	0.29470849444633984
2225	78-79	0.45742646040150703
2225	80-81	0.27706537911657136
2225	82-83	0.16925568687203452
2225	84-85	0.11250447861752377
2225	86-87	0.18552967536312792
2225	88-89	0.07755616468168114
2225	90-91	0.12076427534410783
2225	92-93	0.21581757073266772
2225	94-95	0.3530058381458687
2225	96-97	0.37259498403373215
2225	98-99	0.4164803936919128
2225	100	0.45928430661929553
2226	1	1.3670827159348384
2226	2	1.4451923557729707
2226	3	0.7486584879672584
2226	4	1.1891753861451733
2226	5	2.9926421619455468
2226	6	-0.3962118162141621
2226	7	-0.10853497884243524
2226	8	0.02540870554888386
2226	9	-0.10431698729793482
2226	10-11	0.002923182268915525
2226	12-13	-0.03685972321914477
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2226	16-17	-0.0867221540012082
2226	18-19	-0.09358738850962567
2226	20-21	-0.05308289935469901
2226	22-23	-0.0673443750247884
2226	24-25	-0.014975395837907968
2226	26-27	-0.13727308984833542
2226	28-29	-0.0029407789419408914
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2226	72-73	0.14437439275015862
2226	74-75	0.035901954092203425
2226	76-77	0.24239923560530485
2226	78-79	0.09718203252762692
2226	80-81	0.12000042484357465
2226	82-83	-0.04473430199149675
2226	84-85	-0.043924639343330796
2226	86-87	0.05724398026862332
2226	88-89	0.08374473890270906
2226	90-91	0.2597611920490621
2226	92-93	0.05258294372634964
2226	94-95	0.34487357445826916
2226	96-97	0.3101151834776843
2226	98-99	0.16295344098165288
2226	100	0.3541874920634953
2227	1	1.3271810144623348
2227	2	1.5741532469076809
2227	3	0.5246723971431049
2227	4	0.006846915101787587
2227	5	3.2324035544422216
2227	6	0.08010146264046725
2227	7	0.2609209249908133
2227	8	0.27678300976111814
2227	9	0.2248401390163366
2227	10-11	0.17700097561830574
2227	12-13	-0.00954972427187073
2227	14-15	0.14006535999619274
2227	16-17	0.07195208244161932
2227	18-19	0.10962565413903747
2227	20-21	0.08944478411614654
2227	22-23	0.07740497688920556
2227	24-25	0.10293176337412291
2227	26-27	0.14678300742378525
2227	28-29	0.06065800647250086
2227	30-31	0.06011418576509442
2227	32-33	0.06322962081581807
2227	34-35	0.1748411311727054
2227	36-37	0.13786466245553441
2227	38-39	0.18046222644642995
2227	40-41	0.1580388362572691
2227	42-43	0.2675599884262354
2227	44-45	0.2191653837080807
2227	46-47	0.11211346159198143
2227	48-49	0.04998056600248191
2227	50-51	0.21352573079820303
2227	52-53	0.19944649591305819
2227	54-55	0.3562183740815854
2227	56-57	0.26572877753270774
2227	58-59	0.3533488133136373
2227	60-61	0.1668770198322349
2227	62-63	0.20559097125187975
2227	64-65	0.19115916825629853
2227	66-67	0.22079201824043793
2227	68-69	0.06972707860458627
2227	70-71	0.3346964416276492
2227	72-73	0.18113740052719152
2227	74-75	0.08180248111442268
2227	76-77	0.30290118973266544
2227	78-79	0.282493755317887
2227	80-81	0.08111912985587111
2227	82-83	0.14407239730975618
2227	84-85	0.16291357272882578
2227	86-87	0.10811864964605888
2227	88-89	0.2746716170362191
2227	90-91	0.1950781784820279
2227	92-93	0.2148344833418463
2227	94-95	0.39487941381189984
2227	96-97	0.3644722459702834
2227	98-99	0.29288621439498996
2227	100	0.4419429432234736
2228	1	1.4053960720992222
2228	2	1.4907312204471097
2228	3	-0.13456587898998507
2228	4	-0.09865151298309627
2228	5	3.158822371576097
2228	6	0.18533135522626765
2228	7	0.16785045079641492
2228	8	0.3156134066696836
2228	9	0.22846373084237115
2228	10-11	0.20083595727929549
2228	12-13	0.29678623983606656
2228	14-15	0.1920408970671872
2228	16-17	0.0949740892532489
2228	18-19	0.10953436844346243
2228	20-21	0.12079630363409422
2228	22-23	0.2819246422022772
2228	24-25	0.30564454500891713
2228	26-27	0.21327174203211996
2228	28-29	0.29229648236980665
2228	30-31	0.2709726806563708
2228	32-33	0.23113226032328527
2228	34-35	0.2461418090611076
2228	36-37	0.3040119967494732
2228	38-39	0.16358460658789653
2228	40-41	0.22865876028136967
2228	42-43	0.4455724163801307
2228	44-45	0.3361256928513825
2228	46-47	0.3157018490690575
2228	48-49	0.34226303587410456
2228	50-51	0.2241459823046199
2228	52-53	0.2980227665444417
2228	54-55	0.41133078238654264
2228	56-57	0.5327271564952483
2228	58-59	0.5179594368399663
2228	60-61	0.4383872864028362
2228	62-63	0.49821115726183507
2228	64-65	0.47280337509413073
2228	66-67	0.6003579404306336
2228	68-69	0.5176696709545965
2228	70-71	0.4986791178131327
2228	72-73	0.47142918727070793
2228	74-75	0.40506641548696365
2228	76-77	0.6064695189178906
2228	78-79	0.5368419017679855
2228	80-81	0.3790887394969502
2228	82-83	0.32876546450912514
2228	84-85	0.46470121682104804
2228	86-87	0.4707168943408533
2228	88-89	0.4643993154905104
2228	90-91	0.5313955579685299
2228	92-93	0.5143960663913631
2228	94-95	0.6900998094105333
2228	96-97	0.5652463322946204
2228	98-99	0.7036296811227913
2228	100	0.6474847627152158
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	38.0
13	43.0
14	186.0
15	663.0
16	2427.0
17	6127.0
18	10163.0
19	12955.0
20	15527.0
21	17763.0
22	20403.0
23	23325.0
24	26428.0
25	30342.0
26	34157.0
27	38485.0
28	43871.0
29	50104.0
30	56605.0
31	65966.0
32	77362.0
33	92430.0
34	114128.0
35	147248.0
36	206733.0
37	342708.0
38	839302.0
39	1612607.0
40	2022.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.55010980886734	17.995810592388032	20.11283857734096	36.34124102140367
2	19.267293969328634	49.64908961248696	14.912303252386577	16.171313165797834
3	41.000375824075256	15.749496544834887	23.029789841850558	20.2203377892393
4	17.9806627973753	15.902808089626072	22.142284629926394	43.97424448307223
5	17.155572144598185	34.27464154043656	26.794071542302827	21.775714772662422
6	18.292041526760887	28.776067975315918	29.207031766131514	23.724858731791684
7	20.52410749493974	26.25961474690485	35.26887873324151	17.947399024913896
8	21.925453161060922	28.465434724602183	32.10792063376998	17.501191480566913
9	21.72985498126278	28.414896411882623	26.46827165654101	23.38697695031359
10-11	19.810183135832897	30.013742513723233	28.72496412705219	21.45111022339168
12-13	23.819932968614317	26.916265779084338	27.203814897131657	22.059986355169688
14-15	22.12010021289843	27.541850915576337	28.28642987179309	22.051618999732142
16-17	20.81785179781179	29.4244930359439	28.470743046869014	21.286912119375298
18-19	21.47627655510707	29.61633811622167	29.4202386662821	19.48714666238916
20-21	22.880706960560065	28.92392981395423	29.60926892191959	18.586094303566114
22-23	21.632955607002152	29.915249357474504	28.751659975352933	19.700135060170414
24-25	22.31572409885767	28.388663274481647	26.400613040529876	22.894999586130808
26-27	20.3151678175315	26.75264606369267	29.83606152821071	23.096124590565122
28-29	19.854291888190193	27.813817926812277	30.39143406375045	21.94045612124708
30-31	20.286688610803	29.87507730301277	28.173594769091032	21.664639317093197
32-33	21.264496346897445	29.74402061839769	28.40010251617046	20.5913805185344
34-35	21.406792801657947	28.70486190907319	30.253606702932917	19.634738586335942
36-37	22.20854226015766	28.106988014245328	28.48952139755144	21.194948328045577
38-39	21.598347591823305	29.135390531814252	28.926975794842264	20.339286081520175
40-41	20.931511906105378	29.513596566430927	29.720019958545656	19.834871568918036
42-43	21.057920289472275	29.509498887371794	29.016826886927593	20.415753936228338
44-45	20.800320195942643	29.980825774436664	28.450807918937166	20.76804611068353
46-47	20.318391065358792	28.046999667740135	28.732097652265953	22.902511614635117
48-49	21.301207482807396	28.31581565338322	29.843918596289186	20.539058267520197
50-51	22.286894639185753	30.54990876883426	27.872277910335878	19.290918681644104
52-53	20.543344083815718	30.004925376433977	28.27256046053812	21.179170079212188
54-55	22.06184949414778	28.586519126327943	27.767274810335945	21.58435656918833
56-57	21.249676545821742	28.905510759374643	29.045368939580623	20.799443755222992
58-59	21.1502331696169	28.06307566902654	28.91948568349993	21.867205477856633
60-61	20.04792672075346	30.017720599094293	29.423520775978957	20.510831904173294
62-63	20.480434875664695	30.920772452424227	27.119273942334598	21.47951872957648
64-65	20.21529706009863	30.03592311638208	28.640938111445845	21.107841712073437
66-67	20.363765315088127	29.92745978399627	29.282749263647016	20.426025637268587
68-69	21.102136233399605	29.70001167059714	28.92963658171809	20.26821551428517
70-71	19.51840535428488	30.47798293008078	29.686464523698252	20.31714719193608
72-73	20.469931760424746	30.371623688535927	28.30406429830663	20.854380252732692
74-75	20.413378206008147	29.01181917874985	30.04992393546931	20.524878679772694
76-77	19.887751476947486	29.999398475830297	28.772404847359383	21.34044519986283
78-79	19.75956770463004	29.261605946143536	28.26245887656878	22.71636747265764
80-81	21.012872890838903	29.36808005320154	28.27365169083777	21.34539536512178
82-83	20.355462225053326	28.343266193981776	29.816062134876116	21.48520944608878
84-85	20.236018547509353	29.396241964896696	28.25838445003468	22.109355037559272
86-87	21.973369445348446	28.26662327466673	28.975457813876083	20.78454946610874
88-89	21.508242166432996	27.74189626124452	29.160092315965734	21.589769256356746
90-91	21.24247901991662	27.893177019308922	28.77201925494291	22.092324705831544
92-93	21.494527929486974	28.51009917951075	28.75504033553738	21.2403325554649
94-95	21.02121323826167	29.36047184172819	28.393380355043213	21.224934564966922
96-97	20.97512209141214	28.976293264111785	28.88168173818892	21.16690290628716
98-99	22.17896732181389	28.7580479563859	28.234811900307395	20.828172821492817
100	22.547182373388157	30.189212769381292	26.846512110943678	20.417092746286876
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	13.5
2	28.5
3	29.0
4	31.0
5	38.5
6	45.5
7	57.5
8	64.5
9	74.5
10	95.5
11	107.0
12	123.5
13	147.5
14	180.5
15	263.0
16	347.5
17	457.0
18	661.5
19	1045.5
20	1705.5
21	2646.5
22	3968.0
23	6066.5
24	9463.5
25	14121.0
26	20084.5
27	27151.0
28	35184.5
29	44341.5
30	55125.5
31	67277.0
32	80843.0
33	95796.0
34	111864.0
35	129413.0
36	147100.5
37	161084.0
38	170349.0
39	177619.5
40	182446.0
41	183745.5
42	182645.0
43	180778.0
44	179081.0
45	174327.0
46	163411.0
47	152436.0
48	146364.5
49	140066.0
50	131995.0
51	121589.5
52	108152.5
53	94498.5
54	80171.5
55	64155.0
56	48791.5
57	36361.5
58	26365.0
59	18988.0
60	14659.5
61	12546.0
62	10811.0
63	8479.0
64	6280.0
65	5927.5
66	7835.5
67	8114.5
68	5463.5
69	5049.0
70	7430.0
71	7535.5
72	4299.0
73	1500.0
74	623.0
75	420.0
76	300.5
77	229.0
78	171.0
79	120.5
80	96.0
81	70.0
82	47.0
83	33.5
84	30.5
85	30.5
86	27.5
87	26.5
88	25.5
89	25.5
90	19.0
91	13.5
92	16.5
93	17.5
94	19.5
95	22.0
96	22.0
97	29.0
98	56.5
99	134.0
100	136.5
>>END_MODULE
>>Per base N content	warn
#Base	N-Count
1	11.334951793236092
2	0.006066654019235406
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	3.0847393318146133E-4
30-31	0.01008966823114363
32-33	0.0
34-35	0.0
36-37	0.0
38-39	9.768341217412941E-4
40-41	0.001413838860415031
42-43	1.15677724943048E-4
44-45	0.0
46-47	0.0023264075794101878
48-49	1.2853080549227555E-5
50-51	0.0
52-53	0.0015423696659073065
54-55	0.01904826537395524
56-57	0.011914805669133943
58-59	0.014588246423373277
60-61	3.213270137306889E-4
62-63	0.011683450219247847
64-65	1.15677724943048E-4
66-67	0.0
68-69	0.0
70-71	0.0
72-73	0.0
74-75	0.0
76-77	0.0
78-79	0.0
80-81	1.2853080549227555E-4
82-83	0.0
84-85	0.0
86-87	0.0
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3890118.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.31287151726106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.20185713647555	60.759890910055134
2	9.094197306545304	12.970666481485734
3	2.5350738669507344	5.423501908818716
4	1.0714945170139185	3.0564540329305308
5	0.6025101787947718	2.148336548411677
6	0.34619951667541676	1.4813088991207122
7	0.23367222474352678	1.166468613820137
8	0.17483900234543126	0.9974617048372674
9	0.12290286727623323	0.7888100744855707
>10	0.5677909593834264	6.951546073116253
>50	0.0330584691265467	1.604528461451564
>100	0.01526613437503373	1.981872898661618
>500	8.808931619083496E-4	0.3990578198293261
>1k	2.569271320832829E-4	0.27009557297576064
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	2.570616109845511E-5	0.0	0.0	0.0	0.0
14-15	2.570616109845511E-5	0.0	0.0	0.0	0.0
16-17	2.570616109845511E-5	0.0	0.0	0.0	0.0
18-19	2.570616109845511E-5	0.0	0.0	0.0	0.0
20-21	2.570616109845511E-5	0.0	0.0	0.0	0.0
22-23	2.570616109845511E-5	2.570616109845511E-5	0.0	0.0	0.0
24-25	2.570616109845511E-5	2.570616109845511E-5	0.0	0.0	0.0
26-27	2.570616109845511E-5	2.570616109845511E-5	0.0	0.0	0.0
28-29	2.570616109845511E-5	2.570616109845511E-5	0.0	0.0	0.0
30-31	2.570616109845511E-5	2.570616109845511E-5	0.0	0.0	0.0
32-33	2.570616109845511E-5	2.570616109845511E-5	0.0	0.0	0.0
34-35	3.8559241647682666E-5	2.570616109845511E-5	0.0	0.0	0.0
36-37	5.141232219691022E-5	2.570616109845511E-5	0.0	0.0	0.0
38-39	5.141232219691022E-5	2.570616109845511E-5	0.0	0.0	0.0
40-41	5.141232219691022E-5	2.570616109845511E-5	0.0	0.0	0.0
42-43	5.141232219691022E-5	2.570616109845511E-5	0.0	0.0	0.0
44-45	5.141232219691022E-5	2.570616109845511E-5	0.0	0.0	0.0
46-47	5.141232219691022E-5	2.570616109845511E-5	0.0	0.0	1.2853080549227555E-5
48-49	5.141232219691022E-5	2.570616109845511E-5	0.0	0.0	2.570616109845511E-5
50-51	5.141232219691022E-5	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
52-53	1.413838860415031E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
54-55	2.0564928878764088E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
56-57	2.1850236933686844E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
58-59	2.31355449886096E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
60-61	2.6991469153377865E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
62-63	2.827677720830062E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
64-65	3.0847393318146133E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
66-67	3.3418009427991645E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
68-69	3.984454970260542E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
70-71	4.4985781922296444E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
72-73	4.7556398032141955E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
74-75	5.398293830675573E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
76-77	6.169478663629227E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
78-79	7.454786718551982E-4	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
80-81	0.0010925118466843422	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
82-83	0.0012338957327258453	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
84-85	0.0014395450215134863	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
86-87	0.0015423696659073068	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
88	0.0015423696659073068	2.570616109845511E-5	0.0	2.570616109845511E-5	2.570616109845511E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCGG	13830	0.0	79.8253	8
CTTCACC	15505	0.0	73.44199	6
CACCGGC	15040	0.0	71.99879	9
GAGCTGC	60	1.2551027E-10	70.62705	1
CGCTGCA	90	0.0	70.62705	1
CCTTCAC	17195	0.0	69.03544	5
CGGGCCC	1210	0.0	68.661514	7
CGGGGCA	1400	0.0	68.39551	94
TTCACCG	16725	0.0	67.35511	7
GGCGCGC	1135	0.0	66.99534	6
CGTAGTG	2995	0.0	65.82304	4
GGGCCCG	1285	0.0	64.65403	8
CAGCTGC	140	0.0	64.32107	1
AACGGGC	1275	0.0	64.056694	5
AGGCGCG	1210	0.0	62.84274	5
GCGCGCA	1215	0.0	62.197807	7
GTGTGGC	5665	0.0	61.97651	7
GGCCCGA	1380	0.0	60.54334	9
ATGCAGC	110	0.0	59.743233	2
ACGGGCC	1400	0.0	59.34316	6
>>END_MODULE