FastQCFastQC Report
Tue 7 Mar 2017
1NF_14A_1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_14A_1.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2719198
Sequences flagged as poor quality0
Sequence length95
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACATAA154530.5682925627335706No Hit
CAGCGTAGTGTGATCGTCTATAAATTAGGGACGATTTTAGTACGACTGTG69900.2570610893358998No Hit
CAGCCCTTCACCGGCAATGGTGACGTCAAATATTTTCGTGTGAGACGTAA63490.23348796225946034No Hit
CAGCACTGGAGCCACTTAAGGGTTTACGGAGCACTTGCCCCGACCTAGTT62090.22833938536289008No Hit
CAGCAACGGGCCCGAGCAATTCACATTCCATCCAGAGTTCGTACAAACCA61390.22576509691460497No Hit
CAGCATAAATCCCGATCTCACTATCGTTGGTTACATGCCTATCGTACAAG55020.20233907203521037No Hit
CAGCCCTTCACCGGCAATGGTGACGTCTCCATATGAGTGAAATATTCTCG54460.20027964127658227No Hit
CAGCCCTTCACCGGCATTGGTGACGTCTCCATATGAGTGAAATATTCTCG50980.18748175013367913No Hit
CAGCTCTGTGTATATATAATTAACCGCTTTGGAAACTGCCAGTTCATTTT46810.17214634609175206No Hit
CAGCGTACATTTTGTGGACAAAGAGTGAAAGACAGGTATGTACTGACCAC44830.16486478733803128No Hit
CAGCGTAGTGAGGTCGTGGGAAGAGCGCACGGCGGTCGGGTACTATCGTA43260.15909102610402037No Hit
CAGCATGTGTGGCACGCAAAAGAACCCTAAAAAAAGCCATGAGTGCTCTA42500.15629608436016795No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAAGCCATGAGTGCTCT38410.1412548847123306No Hit
CAGCAGGGTCAGTAGGTAACGATGGGTGTCACGAGTTGCCTAATCAGTGC38210.14051937372710632No Hit
CAGCCTCGGGTTCTGTCTAGGTGGGATCCTCGGTCCAGCGCGGCCGGGGC37310.13720957429359687No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTAAAAAAAGCCATGAGTGCTCTA36990.13603275671723794No Hit
CAGCTGAGTTCAGCTGAAAGAGTACTCTGTCACAGGAAATGTCACAGTGA34510.1269124205004564No Hit
CTGCGGACCTCCATCAGAGTTTCCTCTGACTTCGTCCTACTCAAGCATAG34420.12658144055710543No Hit
CAGCGATCAAATCGCTTACATTCCCTAATTTAACTCGCAATGCCTCGTCA32480.11944698400042954No Hit
CAGCTACTACCCTTGCACAGCAAGGCATTGCTAATAAGTTATTCAAGCGC28890.106244561815653No Hit
CAGCATGTGTGGCACGCAAAAGTACCCTTAAAAAAAAGCCATGAGTGCTC27620.10157406705947856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTGCTT256.8163354E-788.999873
GCTGCTC158.577967E-488.999873
GCTGCCG256.8163354E-788.999873
GCTGCAT500.088.999873
CTGCAGC600.088.999871
AGCTGCT1300.088.999872
CAGCTGC5350.088.999871
GCAGCTA202.4113282E-588.999873
GCAGCAT301.9388608E-888.999873
TGCAGCA158.577967E-488.999872
CAGCAGC4200.087.9403461
AGCTGCC2450.087.1835562
AGCAGCA1500.086.033212
AGCAGCG1150.085.130322
AGCAGCC900.084.0554352
CAGCGTA65050.083.732391
CAGCCCT164200.082.983441
AGCTGCA1300.082.1537252
GCTGCCC1750.081.3713153
AGCCCTT140900.080.630472
Produced by FastQC (version 0.11.5)