FastQCFastQC Report
Tue 7 Mar 2017
1NF_14A_2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename1NF_14A_2.fq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2719198
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGG115150.080.586978
CACCGGC115100.078.338299
CGCTGCA850.074.771581
CTTCACC131050.073.889766
CCTTCAC143600.071.517945
GCTGCCG307.5927645E-570.61761
GTGTGGC48600.070.020277
CGGGGCA12200.069.63711594
CGTAGTG21800.069.545354
ACGCAGC353.6670572E-667.058832
TTCACCG142950.066.9835367
ATGCAGC850.066.2699052
ACCTTCA56150.064.116194
CAGCTGC751.2732926E-1163.5558431
TGTGGCA55050.061.7310038
GCCTTCA43750.061.367734
GCGTAGT14750.060.143943
CAGCGTA10200.059.713411
CGGGCCC7350.059.3905947
TGTGTGG53750.058.9450536
Produced by FastQC (version 0.11.5)